Takemura, Yasushi, Aoki, T., Tanaka, H., Yamada, T., Abe, S., Kohno, S., ...
A fast magnetization reversal in twisted Vicalloy wires is utilized in speed sensor, rotation sensor, and other applications. It has the advantage of generating a constant pulse voltage in a pickup...
Gene transfer using nonviral delivery systems (2006)
Miyazaki, M, Obata, Y, Abe, K, Furusu, A, Koji, T, Tabata, Y, ...
Response of biological cell exposed on burst RF fields (2005)
Nomura, Naoyuki, Koga, Y., Abe, S., Uchida, Ichiro, Abe, K., Katsuki, Sunao, ...
Burst RF fields (BRFFs) were applied to Chinese Hamster Ovary (CHO) cells, and the cell response to the BRFF of two different frequencies, 20 kHz and 50 MHz, was investigated by means of the...
Trends in antimicrobial resistance of Streptococcus pneumoniae in Japan.
Yoshida, R, Kaku, M, Kohno, S, Ishida, K, Mizukane, R, Takemura, H, ...
A total of 184 isolates of Streptococcus pneumoniae were recovered from the sputa of patients over a 5-year period in the Nagasaki area and were examined. A total of 30 strains were resistant to...
Ohno, H, Koga, H, Kohno, S, Tashiro, T, Hara, K
We analyzed the relationship between rifampin MICs and rpoB mutations of 40 clinical isolates of Mycobacterium tuberculosis. A point mutation in either codon 516, 526, or 531 was found in 13 strains...
Fujii, T, Kadota, J, Morikawa, T, Matsubara, Y, Kawakami, K, Iida, K, ...
We have recently reported that long-term administration of erythromycin at a low dose reduced the number of neutrophils and concentrations of interleukin 8 (IL-8) in bronchoalveolar lavage fluid in...
Takemura, H, Kaku, M, Kohno, S, Hirakata, Y, Tanaka, H, Yoshida, R, ...
Defensins are small cationic bactericidal peptides present abundantly in the granules of polymorphonuclear neutrophils (PMNs). Human PMNs contain four defensins termed HNP-1 to HNP-4. We used a new...
Sawai, T, Tomono, K, Yanagihara, K, Yamamoto, Y, Kaku, M, Hirakata, Y, ...
We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of...
Kakeya, H, Udono, H, Ikuno, N, Yamamoto, Y, Mitsutake, K, Miyazaki, T, ...
Heat shock proteins (HSPs) from several pathogenic microbes have been shown to be target molecules of humoral responses as well as cellular immune responses. However, little is known about target...
Efficacy of NY-198 against experimental Legionnaires disease.
Kohno, S, Yamaguchi, K, Dohtsu, Y, Koga, H, Hayashi, T, Hirota, M, ...
The in vitro and in vivo effects of NY-198 against Legionella pneumophila were compared with those of ciprofloxacin. The MIC of NY-198 against 15 standard reference strains of Legionella of various...
Accumulation of a newly developed fluoroquinolone, OPC-17116, by human polymorphonuclear leukocytes.
The accumulation of OPC-17116 by human polymorphonuclear leukocytes was studied by comparison with three other new quinolones, ofloxacin, levofloxacin, and DR-3354. The intracellular/extracellular...
Maesaki, S, Kohno, S, Kaku, M, Koga, H, Hara, K
The in vitro effects of antifungal agent combinations administered simultaneously and sequentially against 15 strains of Aspergillus fumigatus were tested by the yeast-malt broth method. The...
Efficacy of intravenous itraconazole against experimental pulmonary aspergillosis.
Miyazaki, H M, Kohno, S, Miyazaki, Y, Mitsutake, K, Tomono, K, Kaku, M, ...
The efficacy of intravenous itraconazole solubilized in hydroxypropyl-beta-cyclodextrin was assessed in a rat model of Aspergillus fumigatus pneumonia. Immunosuppressed rats were infected by...
Ohtsubo, S, Demizu, K, Kohno, S, Miura, I, Ogawa, T, Fukuda, H
The kinetics of acetate utilization by concentrated suspensions of cells was examined in five strains of Methanothrix soehngenii. The rate of acetate utilization by all strains was dependent on the...
Miyazaki, T, Kohno, S, Mitsutake, K, Maesaki, S, Tanaka, K, Ishikawa, N, ...
(1-->3)-beta-D-Glucan is one of the major structural components of fungi, and it seems that it can be detected by the fractionated (1-->3)-beta-D-glucan-sensitive component from a Limulus lysate,...
Yamamoto, Y, Kohno, S, Koga, H, Kakeya, H, Tomono, K, Kaku, M, ...
We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern...
Miyamoto, J, Koga, H, Kohno, S, Tashiro, T, Hara, K
We developed a novel method for early detection of drug-resistant strains of Mycobacterium tuberculosis by using the hybridization protection assay (HPA). The number of viable bacteria during the...
Enolase antigen, mannan antigen, Cand-Tec antigen, and beta-glucan in patients with candidemia.
Mitsutake, K, Miyazaki, T, Tashiro, T, Yamamoto, Y, Kakeya, H, Otsubo, T, ...
We compared the specificities and sensitivities of four tests used for the serodiagnosis of candidemia in 39 patients with candidemia, including 10 patients with superficial Candida colonization, 10...
Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis.
Tanaka, K, Miyazaki, T, Maesaki, S, Mitsutake, K, Kakeya, H, Yamamoto, Y, ...
Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was...
Double-stranded RNA virus in the human pathogenic fungus Blastomyces dermatitidis.
Kohno, S, Fujimura, T, Rulong, S, Kwon-Chung, K J
Double-stranded RNA viruses were detected in a strain of Blastomyces dermatitidis isolated from a patient in Uganda. The viral particles are spherical (mostly 44 to 50 nm in diameter) and consist of...
Matsumura, H, Futaesaku, Y, Kohno, S, Sugiura, A, Kohase, M
A temperature-sensitive mutant (ts3) of Newcastle disease virus was physiologically characterized. All major viral structural proteins were synthesized at the permissive (37 degrees C) and...
Detection of Bacteroides fragilis in clinical specimens by PCR.
Yamashita, Y, Kohno, S, Koga, H, Tomono, K, Kaku, M
The direct detection of Bacteroides fragilis from clinical specimens was examined by using the PCR method for amplifying a specific fragment of the glutamine synthetase gene from B. fragilis. By this...
Rapid and highly reproducible method for antifungal susceptibility testing of Aspergillus species.
Yamada, H, Kohno, S, Maesaki, S, Koga, H, Kaku, M, Hara, K, ...
A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high...
Pneumonia caused by Stenotrophomonas maltophilia with a mucoid phenotype.
Irifune, K, Ishida, T, Shimoguchi, K, Ohtake, J, Tanaka, T, Morikawa, N, ...
We describe the first known case of pneumonia caused by a mucoid Stenotrophomonas maltophilia (Xanthomonas maltophilia) strain in a patient with bronchiectasis. The patient was admitted because of...
Matsuda, H, Kohno, S, Maesaki, S, Yamada, H, Koga, H, Tamura, M, ...
The ubiquinone systems and electrophoretic comparison of enzymes were used to determine the relatedness among 64 isolates of seven Aspergillus spp. These were 31 clinical and 3 nonclinical isolates...
Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.
Miyazaki, Y, Koga, H, Kohno, S, Kaku, M
The nested polymerase chain reaction technique was compared with the conventional smear and culture methods for detection of Mycobacterium tuberculosis. The nested polymerase chain reaction used in...
Mizukane, R, Hirakata, Y, Kaku, M, Ishii, Y, Furuya, N, Ishida, K, ...
The inhibitory effects of azithromycin (AZM), a new 15-membered macrolide antibiotic, on the production of exotoxin A, total protease, elastase, and phospholipase C by Pseudomonas aeruginosa were...
Virulence of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa In Vitro and In Vivo
Aoki, S., Hirakata, Y., Kondoh, A., Gotoh, N., Yanagihara, K., Miyazaki, Y., ...
We evaluated the virulence of Pseudomonas aeruginosa carrying blaIMP, a metallo-β-lactamase gene, and the efficacy of ceftazidime, imipenem-cilastatin, and ciprofloxacin in the endogenous bacteremia...
Matsumoto, K, Tajima, H, Hamanoue, M, Kohno, S, Kinoshita, T, Nakamura, T
The marked and rapid increase of hepatocyte growth factor (HGF) mRNA in the intact lung of rats after partial hepatectomy or unilateral nephrectomy suggests the existence of a humoral factor...
Trends in antimicrobial resistance of Streptococcus pneumoniae in Japan.
Yoshida, R, Kaku, M, Kohno, S, Ishida, K, Mizukane, R, Takemura, H, ...
A total of 184 isolates of Streptococcus pneumoniae were recovered from the sputa of patients over a 5-year period in the Nagasaki area and were examined. A total of 30 strains were resistant to...
Ohno, H, Koga, H, Kohno, S, Tashiro, T, Hara, K
We analyzed the relationship between rifampin MICs and rpoB mutations of 40 clinical isolates of Mycobacterium tuberculosis. A point mutation in either codon 516, 526, or 531 was found in 13 strains...
Fujii, T, Kadota, J, Morikawa, T, Matsubara, Y, Kawakami, K, Iida, K, ...
We have recently reported that long-term administration of erythromycin at a low dose reduced the number of neutrophils and concentrations of interleukin 8 (IL-8) in bronchoalveolar lavage fluid in...
Takemura, H, Kaku, M, Kohno, S, Hirakata, Y, Tanaka, H, Yoshida, R, ...
Defensins are small cationic bactericidal peptides present abundantly in the granules of polymorphonuclear neutrophils (PMNs). Human PMNs contain four defensins termed HNP-1 to HNP-4. We used a new...
Sawai, T, Tomono, K, Yanagihara, K, Yamamoto, Y, Kaku, M, Hirakata, Y, ...
We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of...
Kakeya, H, Udono, H, Ikuno, N, Yamamoto, Y, Mitsutake, K, Miyazaki, T, ...
Heat shock proteins (HSPs) from several pathogenic microbes have been shown to be target molecules of humoral responses as well as cellular immune responses. However, little is known about target...
Efficacy of NY-198 against experimental Legionnaires disease.
Kohno, S, Yamaguchi, K, Dohtsu, Y, Koga, H, Hayashi, T, Hirota, M, ...
The in vitro and in vivo effects of NY-198 against Legionella pneumophila were compared with those of ciprofloxacin. The MIC of NY-198 against 15 standard reference strains of Legionella of various...
Accumulation of a newly developed fluoroquinolone, OPC-17116, by human polymorphonuclear leukocytes.
The accumulation of OPC-17116 by human polymorphonuclear leukocytes was studied by comparison with three other new quinolones, ofloxacin, levofloxacin, and DR-3354. The intracellular/extracellular...
Maesaki, S, Kohno, S, Kaku, M, Koga, H, Hara, K
The in vitro effects of antifungal agent combinations administered simultaneously and sequentially against 15 strains of Aspergillus fumigatus were tested by the yeast-malt broth method. The...
Efficacy of intravenous itraconazole against experimental pulmonary aspergillosis.
Miyazaki, H M, Kohno, S, Miyazaki, Y, Mitsutake, K, Tomono, K, Kaku, M, ...
The efficacy of intravenous itraconazole solubilized in hydroxypropyl-beta-cyclodextrin was assessed in a rat model of Aspergillus fumigatus pneumonia. Immunosuppressed rats were infected by...
Ohtsubo, S, Demizu, K, Kohno, S, Miura, I, Ogawa, T, Fukuda, H
The kinetics of acetate utilization by concentrated suspensions of cells was examined in five strains of Methanothrix soehngenii. The rate of acetate utilization by all strains was dependent on the...
Miyazaki, T, Kohno, S, Mitsutake, K, Maesaki, S, Tanaka, K, Ishikawa, N, ...
(1-->3)-beta-D-Glucan is one of the major structural components of fungi, and it seems that it can be detected by the fractionated (1-->3)-beta-D-glucan-sensitive component from a Limulus lysate,...
Yamamoto, Y, Kohno, S, Koga, H, Kakeya, H, Tomono, K, Kaku, M, ...
We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern...
Miyamoto, J, Koga, H, Kohno, S, Tashiro, T, Hara, K
We developed a novel method for early detection of drug-resistant strains of Mycobacterium tuberculosis by using the hybridization protection assay (HPA). The number of viable bacteria during the...
Enolase antigen, mannan antigen, Cand-Tec antigen, and beta-glucan in patients with candidemia.
Mitsutake, K, Miyazaki, T, Tashiro, T, Yamamoto, Y, Kakeya, H, Otsubo, T, ...
We compared the specificities and sensitivities of four tests used for the serodiagnosis of candidemia in 39 patients with candidemia, including 10 patients with superficial Candida colonization, 10...
Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis.
Tanaka, K, Miyazaki, T, Maesaki, S, Mitsutake, K, Kakeya, H, Yamamoto, Y, ...
Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was...
Double-stranded RNA virus in the human pathogenic fungus Blastomyces dermatitidis.
Kohno, S, Fujimura, T, Rulong, S, Kwon-Chung, K J
Double-stranded RNA viruses were detected in a strain of Blastomyces dermatitidis isolated from a patient in Uganda. The viral particles are spherical (mostly 44 to 50 nm in diameter) and consist of...
Matsumura, H, Futaesaku, Y, Kohno, S, Sugiura, A, Kohase, M
A temperature-sensitive mutant (ts3) of Newcastle disease virus was physiologically characterized. All major viral structural proteins were synthesized at the permissive (37 degrees C) and...
Detection of Bacteroides fragilis in clinical specimens by PCR.
Yamashita, Y, Kohno, S, Koga, H, Tomono, K, Kaku, M
The direct detection of Bacteroides fragilis from clinical specimens was examined by using the PCR method for amplifying a specific fragment of the glutamine synthetase gene from B. fragilis. By this...
Rapid and highly reproducible method for antifungal susceptibility testing of Aspergillus species.
Yamada, H, Kohno, S, Maesaki, S, Koga, H, Kaku, M, Hara, K, ...
A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high...
Pneumonia caused by Stenotrophomonas maltophilia with a mucoid phenotype.
Irifune, K, Ishida, T, Shimoguchi, K, Ohtake, J, Tanaka, T, Morikawa, N, ...
We describe the first known case of pneumonia caused by a mucoid Stenotrophomonas maltophilia (Xanthomonas maltophilia) strain in a patient with bronchiectasis. The patient was admitted because of...
Matsuda, H, Kohno, S, Maesaki, S, Yamada, H, Koga, H, Tamura, M, ...
The ubiquinone systems and electrophoretic comparison of enzymes were used to determine the relatedness among 64 isolates of seven Aspergillus spp. These were 31 clinical and 3 nonclinical isolates...
Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.
Miyazaki, Y, Koga, H, Kohno, S, Kaku, M
The nested polymerase chain reaction technique was compared with the conventional smear and culture methods for detection of Mycobacterium tuberculosis. The nested polymerase chain reaction used in...
Mizukane, R, Hirakata, Y, Kaku, M, Ishii, Y, Furuya, N, Ishida, K, ...
The inhibitory effects of azithromycin (AZM), a new 15-membered macrolide antibiotic, on the production of exotoxin A, total protease, elastase, and phospholipase C by Pseudomonas aeruginosa were...
Virulence of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa In Vitro and In Vivo
Aoki, S., Hirakata, Y., Kondoh, A., Gotoh, N., Yanagihara, K., Miyazaki, Y., ...
We evaluated the virulence of Pseudomonas aeruginosa carrying blaIMP, a metallo-β-lactamase gene, and the efficacy of ceftazidime, imipenem-cilastatin, and ciprofloxacin in the endogenous bacteremia...