Matsushita, K, Fujimaki, W, Kato, H, Uchiyama, T, Igarashi, H, Ohkuni, H, ...
Previously, we prepared extracellular products, fractions F-1 and F-2 of Streptococcus mitis 108, an isolate from the tooth surface of an infant, and showed that F-1 exhibited inflammatory...
Takata, N, Suginaka, H, Kotani, S, Ogawa, M, Kosaki, G
The intrinsic mechanisms of resistance to beta-lactam antibiotics in Serratia marcescens IFO 12648 were investigated, comparing the action of benzylpenicillin, apalcillin, cefazolin, and ceftizoxime....
Ebisu, S, Kato, K, Kotani, S, Misaki, A
Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their...
Hui, G S, Tam, L Q, Chang, S P, Case, S E, Hashiro, C, Siddiqui, W A, ...
The Plasmodium falciparum major merozoite surface protein (gp195) is a protective antigen against lethal malaria. However, increasing evidence indicates that the efficacy of a malaria vaccine will...
Nagao, S, Nakanishi, M, Kutsukake, H, Yagawa, K, Kusumoto, S, Shiba, T, ...
N-Acetylmuramyl-L-alanyl-D-isoglutamine (muramyl dipeptide [MDP]) injected intraperitoneally significantly increased the number of cells entering the peritoneal cavity of guinea pigs primed with...
Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953.
Takada, H, Ogawa, T, Yoshimura, F, Otsuka, K, Kokeguchi, S, Kato, K, ...
From Fusobacterium nucleatum ATCC 10953 cell envelope fraction whose inner membranes had been removed by treatment with sodium N-lauroyl sarcosinate, an outer membrane protein (37,000 Mr in a native...
Immunobiological activities of synthetic lipid A analogs with low endotoxicity.
Kotani, S, Takada, H, Takahashi, I, Ogawa, T, Tsujimoto, M, Shimauchi, H, ...
Synthetic lipid A analogs, beta(1-6)glucosamine disaccharide 1,4'-bisphosphates, which possesses four tetradecanoyl groups at the 2- and 2'-amino, and 3- and 3'-hydroxyl groups (LA-17-PP), and each...
Takahashi, I, Kotani, S, Takada, H, Tsujimoto, M, Ogawa, T, Shiba, T, ...
Several synthetic acylated glucosamine monophosphates, with structures corresponding to the nonreducing or reducing moiety of the lipid A of the Escherichia coli or Salmonella minnesota type, and a...
A new pharmacological action of N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) is described. Pretreatment of male ddY mice with MDP, but not its biologically inactive analogs, significantly decreased...
Tsujimoto, M, Kotani, S, Kinoshita, F, Kanoh, S, Shiba, T, Kusumoto, S
Thirteen 6-O-acyl-N-acetylmuramyl-L-alanyl-D-isoglutamines (6-O-acyl-MDPs), including four inactive D-isoasparagine and L-isoglutamine analogs, were tested for their pyrogenicity and...
Tsujimoto, M, Kotani, S, Shiba, T, Kusumoto, S
6-O-(2-Tetradecylhexadecanoyl)-N-acetylmuramyl-L-alanyl-D-isoglutamine (B3O-MDP) and 6-O-(3-hydroxy-2-docosylhexacosanoyl)-MDP (BH48-MDP) were examined for immunopotentiating activity in stimulating...
Kotani, S, Takada, H, Takahashi, I, Tsujimoto, M, Ogawa, T, Ikeda, T, ...
A synthetic lipid A (Salmonella type, compound 516), beta (1-6)-linked D-glucosamine disaccharide 1,4'-bisphosphate, with three acyloxyacyl groups and one hydroxyacyl group, i.e.,...
Synthetic Salmonella-type lipid A antigen with high serological specificity.
Kasai, N, Arata, S, Mashimo, J, Okuda, K, Aihara, Y, Kotani, S, ...
A synthetic compound (compound 516), beta(1-6)-linked D-glucosamine disaccharide 1,4'-bisphosphate, which is acylated by (R)-3-hexadecanoyloxytetradecanoyl, (R)-3-hydroxytetradecanoyl,...
Takada, H, Kotani, S, Tsujimoto, M, Ogawa, T, Takahashi, I, Harada, K, ...
Synthetic lipid A analogs (compounds 404 through 406) were examined for their immunopharmacological activities. These compounds had two amide-bound and two ester-bound (R)-3-hydroxytetradecanoyl...
Kotani, S, Takada, H, Tsujimoto, M, Ogawa, T, Takahashi, I, Ikeda, T, ...
A synthetic compound (506), beta (1-6) D-glucosamine disaccharide 1,4'-bisphosphate, which is acylated at 2'-amino and 3'-hydroxyl groups with (R)-3-dodecanoyloxytetradecanoyl and...
Emori, K, Nagao, S, Shigematsu, N, Kotani, S, Tsujimoto, M, Shiba, T, ...
Muramyl dipeptide, which does not induce epithelioid granuloma when injected alone dissolved in phosphate-buffered saline, could induce extensive granulomas in guinea pigs when chemically conjugated...
Koga, T, Kakimoto, K, Hirofuji, T, Kotani, S, Ohkuni, H, Watanabe, K, ...
The systemic injection of an aqueous suspension of cell wall or its peptidoglycan (PG)-rich sonicate derived from group A streptococcus and Lactobacillus casei induced acute joint lesions in BALB/c,...
Kotani, S, Takada, H, Tsujimoto, M, Ogawa, T, Harada, K, Mori, Y, ...
Synthetic lipid A analogs which have two amide-bound and two ester-bound (R)-3-hydroxytetradecanoyl groups at the C-2 and -2' and C-3 and -3' positions of beta(1-6)glucosamine disaccharide mono- or...
Hotta, H, Hotta, S, Takada, H, Kotani, S, Tanaka, S, Ohki, M
The effects of bacterial cell walls, peptidoglycans, and a water-soluble polymer of peptidoglycan subunits on dengue virus type 2 replication in cultured mouse peritoneal macrophages were studied....
Kotani, S, Takada, H, Tsujimoto, M, Ogawa, T, Mori, Y, Sakuta, M, ...
Thirteen acylated and phosphorylated derivatives of beta-1,6-linked glucosamine disaccharide (lipid A analogs), which were synthesized after the structural model of Salmonella-type lipid A, and seven...
Novel biological property of pertussis toxin: chemotactic activity on human monocytes.
Ogawa, T, Takada, H, Kotani, S, Sato, H, Sato, Y
Pertussis toxin isolated from Bordetella pertussis phase I strain Tohama enhanced the migration of human blood monocytes in an assay with a multiwell chemotaxis assembly. The monocyte migration...
Isolation and serotyping of Streptococcus mutans from teeth and feces of children.
Hamada, S, Masuda, N, Kotani, S
Streptococcus mutans were detected in the feces from 10 of 29 caries-active patients, aged 4 to 9 years. The percentage of S. mutans to the total counts of facultatively anaerobic streptococci on...
Isolation and immunobiological classification of Streptococcus sanguis from human tooth surfaces.
Hamada, S, Torii, M, Tsuchitani, Y, Kotani, S
A total of 113 pure cultures of Streptococcus sanguis were obtained from dental plaque samples of 64 subjects. All isolates synthesized glucan from sucrose, elaborated peroxide, and were...
Cytokine induction by extracellular products of oral viridans group streptococci.
Takada, H, Kawabata, Y, Tamura, M, Matsushita, K, Igarashi, H, Ohkuni, H, ...
During an etiological study of Kawasaki disease (mucocutaneous lymph node syndrome [MCLS]), we found that dominant viridans streptococcal strains on tooth surfaces and in the throat of both MCLS...
Somnogenic activities of synthetic lipid A.
Cady, A B, Kotani, S, Shiba, T, Kusumoto, S, Krueger, J M
Bacterial infections and various immune response modifiers, including endotoxin and its lipid A moiety, alter sleep duration. The purpose of this study is to amplify our understanding of lipid A...
Liberation of serotonin from rabbit blood platelets by bacterial cell walls and related compounds.
Harada, K, Kotani, S, Takada, H, Tsujimoto, M, Hirachi, Y, Kusumoto, S, ...
A study was made on the activity of various bacterial cell walls and peptidoglycans to liberate serotonin from rabbit blood platelets. All of the test cell walls or peptidoglycans prepared from 27...
Stimulation of migration of human monocytes by bacterial cell walls and muramyl peptides.
Ogawa, T, Kotani, S, Fukuda, K, Tsukamoto, Y, Mori, M, Kusumoto, S, ...
Bacterial cell walls, water-soluble fragments of the wall peptidoglycan, N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), and 6-O-acyl derivatives of MDP were examined for migration-stimulating...
Fukuhara, H, Umemoto, T, Sagawa, H, Kato, K, Kotani, S
Peptidoglycans of Leptotrichia buccalis ATCC 14201 and ATCC 19616 were isolated by extraction with sodium dodecyl sulfate and subsequent digestion of the sodium dodecyl sulfate-insoluble residue with...
Hamada, S, Torii, M, Kotani, S, Tsuchitani, Y
Streptococcus sanguis isolated from human dental plaque were grown in Todd-Hewitt broth. Cells were collected by centrifugation and lyophilized after extensive washing with water. The cell-associated...
Ogawa, T, Kotani, S, Tsujimoto, M, Kusumoto, S, Shiba, T, Kawata, S, ...
Cell walls isolated from four bacterial species (Streptococcus pyogenes, Lactobacillus plantarum, Streptomyces gardneri, and Nocardia corynebacteriodes), which exhibited the adjuvant effect of...
Kohashi, O, Pearson, C M, Tamaoki, N, Tanaka, A, Shimamura, K, Ozawa, A, ...
A synthetic adjuvant, N-acetyl muramyl-L-alanyl-D-isoglutamine (MDP), produced extremely severe polyarthritis with almost 100% incidence in Rowett euthymic rnu/+ rats, but the same dose of MDP (100...
Chemical and biological properties of a peptidoglycan isolated from Treponema pallidum kazan.
Umemoto, T, Ota, T, Sagawa, H, Kato, K, Takada, H, Tsujimoto, M, ...
A peptidoglycan layer of Treponema pallidum kazan was isolated by solubilization of whole cells with 1% warm sodium dodecyl sulfate and subsequent digestion of an insoluble residue with proteases....
Mizoguchi, J, Suginaka, H, Kotani, S
The mechanism of synergistic activity of a combination of ampicillin and dicloxacillin was studied on beta-lactamase-producing Citrobacter freundii GN346 and its derived beta-lactamaseless mutant...
Stimulation of the reticuloendothelial system of mice by muramyl dipeptide.
Tanaka, A, Nagao, S, Nagao, R, Kotani, S, Shiba, T, Kusumoto, S
Muramyl dipeptide (MDP), a part of bacterial cell wall peptidoglycans, was active as adjuvant and stimulated the reticuloendothelial system (RES) of mice to increase its phagocytic function. A series...
Inhibition of macrophage migration by muramyl peptides.
Nagao, S, Tanaka, A, Yamamoto, Y, Koga, T, Onoue, K, Shiba, T, ...
In the capillary tube migration system a synthetic muramyl dipeptide (MDP; N-acetylmuramyl-L-alanyl-D-isoglutamine), a part of bacterial cell wall peptidoglycans, inhibited the migration of...
Macrophage activation by bacterial cell walls and related synthetic compounds.
Takada, H, Tsujimoto, M, Kato, K, Kotani, S, Kusumoto, S, Inage, M, ...
Activation of peritoneal macrophages from guinea pigs by various bacterial cell walls, M-1 endo-N-acetylmuramidase enzymatically digested bacterial cell walls and synthetic muramyl dipeptides was...
Kohashi, O, Kotani, S, Shiba, T, Ozawa, A
Lewis rats developed polyarthritis after a single injection of a water-in-oil emulsion containing various peptidoglycans (PGs) derived from Lactobacillus plantarum. A copolymer of polyriboinosinic...
Kohashi, O, Pearson, C M, Watanabe, Y, Kotani, S
Cell wall lytic enzyme (Kyowa lytic no. 2 enzyme) liberated arthritogenic hydrosoluble peptidoglycans from both arthritogenic and non-arthritogenic bacterial cell walls. From these cell walls,...
Adjuvant activity of synthetic N-acetylmuramyl peptides in rats.
Tanaka, A, Saito, R, Sugiyama, K, Morisaki, I, Kotani, S
The minimal adjuvant-active structure in bacterial cell wall peptidoglycan for the induction of delayed hypersensitivity in rats (WKA) was the N-acetylmuramyl dipeptides (N-acetylmuramyl...
Kohashi, O, Tanaka, A, Kotani, S, Shiba, T, Kusumoto, S, Yokogawa, K, ...
A synthetic adjuvant, N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), failed to produce polyarthritis with a wide dose range in a water-in-oil emulsion of mineral oil such as liquid paraffin, Drakeol,...
Pabst, M J, Cummings, N P, Shiba, T, Kusumoto, S, Kotani, S
Mouse peritoneal macrophages, when treated with a lipophilic derivative of muramyl dipeptide either in vitro or in vivo by intraperitoneal injection, showed a more than fivefold increase in their...
Immunological activities of Capnocytophaga cellular components.
Murayama, Y, Muranishi, K, Okada, H, Kato, K, Kotani, S, Takada, H, ...
Whole cells of a clinical isolate (strain S-3) of the genus Capnocytophaga were divided into cell envelope (CE) and cytoplasm (CP) fractions by mechanical disintegration followed by differential...
Matsushita, K, Fujimaki, W, Kato, H, Uchiyama, T, Igarashi, H, Ohkuni, H, ...
Previously, we prepared extracellular products, fractions F-1 and F-2 of Streptococcus mitis 108, an isolate from the tooth surface of an infant, and showed that F-1 exhibited inflammatory...
Takata, N, Suginaka, H, Kotani, S, Ogawa, M, Kosaki, G
The intrinsic mechanisms of resistance to beta-lactam antibiotics in Serratia marcescens IFO 12648 were investigated, comparing the action of benzylpenicillin, apalcillin, cefazolin, and ceftizoxime....
Ebisu, S, Kato, K, Kotani, S, Misaki, A
Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their...
Hui, G S, Tam, L Q, Chang, S P, Case, S E, Hashiro, C, Siddiqui, W A, ...
The Plasmodium falciparum major merozoite surface protein (gp195) is a protective antigen against lethal malaria. However, increasing evidence indicates that the efficacy of a malaria vaccine will...
Nagao, S, Nakanishi, M, Kutsukake, H, Yagawa, K, Kusumoto, S, Shiba, T, ...
N-Acetylmuramyl-L-alanyl-D-isoglutamine (muramyl dipeptide [MDP]) injected intraperitoneally significantly increased the number of cells entering the peritoneal cavity of guinea pigs primed with...
Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953.
Takada, H, Ogawa, T, Yoshimura, F, Otsuka, K, Kokeguchi, S, Kato, K, ...
From Fusobacterium nucleatum ATCC 10953 cell envelope fraction whose inner membranes had been removed by treatment with sodium N-lauroyl sarcosinate, an outer membrane protein (37,000 Mr in a native...
Immunobiological activities of synthetic lipid A analogs with low endotoxicity.
Kotani, S, Takada, H, Takahashi, I, Ogawa, T, Tsujimoto, M, Shimauchi, H, ...
Synthetic lipid A analogs, beta(1-6)glucosamine disaccharide 1,4'-bisphosphates, which possesses four tetradecanoyl groups at the 2- and 2'-amino, and 3- and 3'-hydroxyl groups (LA-17-PP), and each...
Takahashi, I, Kotani, S, Takada, H, Tsujimoto, M, Ogawa, T, Shiba, T, ...
Several synthetic acylated glucosamine monophosphates, with structures corresponding to the nonreducing or reducing moiety of the lipid A of the Escherichia coli or Salmonella minnesota type, and a...
A new pharmacological action of N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) is described. Pretreatment of male ddY mice with MDP, but not its biologically inactive analogs, significantly decreased...
Tsujimoto, M, Kotani, S, Kinoshita, F, Kanoh, S, Shiba, T, Kusumoto, S
Thirteen 6-O-acyl-N-acetylmuramyl-L-alanyl-D-isoglutamines (6-O-acyl-MDPs), including four inactive D-isoasparagine and L-isoglutamine analogs, were tested for their pyrogenicity and...
Tsujimoto, M, Kotani, S, Shiba, T, Kusumoto, S
6-O-(2-Tetradecylhexadecanoyl)-N-acetylmuramyl-L-alanyl-D-isoglutamine (B3O-MDP) and 6-O-(3-hydroxy-2-docosylhexacosanoyl)-MDP (BH48-MDP) were examined for immunopotentiating activity in stimulating...
Kotani, S, Takada, H, Takahashi, I, Tsujimoto, M, Ogawa, T, Ikeda, T, ...
A synthetic lipid A (Salmonella type, compound 516), beta (1-6)-linked D-glucosamine disaccharide 1,4'-bisphosphate, with three acyloxyacyl groups and one hydroxyacyl group, i.e.,...
Synthetic Salmonella-type lipid A antigen with high serological specificity.
Kasai, N, Arata, S, Mashimo, J, Okuda, K, Aihara, Y, Kotani, S, ...
A synthetic compound (compound 516), beta(1-6)-linked D-glucosamine disaccharide 1,4'-bisphosphate, which is acylated by (R)-3-hexadecanoyloxytetradecanoyl, (R)-3-hydroxytetradecanoyl,...
Takada, H, Kotani, S, Tsujimoto, M, Ogawa, T, Takahashi, I, Harada, K, ...
Synthetic lipid A analogs (compounds 404 through 406) were examined for their immunopharmacological activities. These compounds had two amide-bound and two ester-bound (R)-3-hydroxytetradecanoyl...
Kotani, S, Takada, H, Tsujimoto, M, Ogawa, T, Takahashi, I, Ikeda, T, ...
A synthetic compound (506), beta (1-6) D-glucosamine disaccharide 1,4'-bisphosphate, which is acylated at 2'-amino and 3'-hydroxyl groups with (R)-3-dodecanoyloxytetradecanoyl and...
Emori, K, Nagao, S, Shigematsu, N, Kotani, S, Tsujimoto, M, Shiba, T, ...
Muramyl dipeptide, which does not induce epithelioid granuloma when injected alone dissolved in phosphate-buffered saline, could induce extensive granulomas in guinea pigs when chemically conjugated...