S. L. Hu

Enhanced immunity to human immunodeficiency virus (HIV) envelope elicited by a combined vaccine regimen consisting of priming with a vaccinia recombinant expressing HIV envelope and boosting with gp160 protein.

Cooney, E L, McElrath, M J, Corey, L, Hu, S L, Collier, A C, Arditti, D, ...

Transmission studies have suggested that an optimal human immunodeficiency virus type 1 (HIV-1) vaccine should induce both neutralizing antibodies and cytolytic T cells to eliminate free virus and...

Sulfhydryl oxidation down-regulates T-cell signaling and inhibits tyrosine phosphorylation of phospholipase C gamma 1.

Kanner, S B, Kavanagh, T J, Grossmann, A, Hu, S L, Bolen, J B, Rabinovitch, P S, ...

Early events in both T-cell receptor (CD3)- and CD4-induced signal transduction pathways include tyrosine phosphorylation of protein substrates, the generation of phosphatidylinositol-phosphate...

Human papillomavirus type 16 nucleoprotein E7 is a tumor rejection antigen.

Chen, L P, Thomas, E K, Hu, S L, Hellström, I, Hellström, K E

It has been speculated that immunological mechanisms play an important role in the control of carcinomas associated with human papillomavirus (HPV), such as cervical cancers. We have now demonstrated...

Detection of simian immunodeficiency virus (SIV)-specific CD8+ T cells in macaques protected from SIV challenge by prior SIV subunit vaccination.

Kent, S J, Hu, S L, Corey, L, Morton, W R, Greenberg, P D

Vaccines for lentiviruses would ideally induce in the host complete resistance to infection of host cells. However, such sterilizing immunity may be neither readily achievable nor absolutely...

Plasma viremia in macaques infected with simian immunodeficiency virus: plasma viral load early in infection predicts survival.

Watson, A, Ranchalis, J, Travis, B, McClure, J, Sutton, W, Johnson, P R, ...

A reliable method for the quantitation of plasma viremia in nonhuman primates infected with simian immunodeficiency virus (SIV) and related viruses is described. This method is based on an...

Inhibition of virus production in peripheral blood mononuclear cells from human immunodeficiency virus (HIV) type 1-seropositive donors by treatment with recombinant HIV-like particles.

Haffar, O K, Moran, P A, Smithgall, M D, Diegel, M L, Sridhar, P, Ledbetter, J A, ...

We have previously reported on the assembly of recombinant human immunodeficiency virus (HIV)-like particles that contain gag structural proteins and present env glycoproteins gp120 and gp41 on their...

Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system.

Haffar, O, Garrigues, J, Travis, B, Moran, P, Zarling, J, Hu, S L

We report the assembly of human immunodeficiency virus (HIV)-like particles in African green monkey kidney cells coinfected with two recombinant vaccinia viruses, one carrying the HIV-1 gag and...

Characterization of a recombinant vaccinia virus expressing human melanoma-associated antigen p97.

Hu, S L, Plowman, G D, Sridhar, P, Stevenson, U S, Brown, J P, Estin, C D

p97 is a cell surface glycoprotein expressed at high levels in most human melanomas but present only in trace amounts in normal adult tissues. We are interested in exploring the possibility of using...

Effects of anti-gp120 monoclonal antibodies on CD4 receptor binding by the env protein of human immunodeficiency virus type 1.

Linsley, P S, Ledbetter, J A, Kinney-Thomas, E, Hu, S L

Monoclonal antibodies (MAbs) to defined peptide epitopes on gp120 from human immunodeficiency virus type 1 were used to investigate the involvement of their epitopes in gp120 binding to the CD4...

Sequence homology between bovine and human adenoviruses.

Hu, S L, Hays, W W, Potts, D E

Cross-hybridization has been detected between corresponding regions of the genomes of bovine adenovirus type 3 and human adenovirus type 2. The most conserved region on the viral genomes encodes the...

Restriction analysis and homology studies of the bovine adenovirus 7 genome.

Hu, S L, Battles, J K, Potts, D E

We located the cleavage sites for restriction endonucleases EcoRI, HindIII, and BamHI on the genome of bovine adenovirus 7. Cross-hybridization at reduced stringency revealed two regions of homology...

Recombinant vaccinia virus vaccine against the human melanoma antigen p97 for use in immunotherapy.

Estin, C D, Stevenson, U S, Plowman, G D, Hu, S L, Sridhar, P, Hellström, I, ...

We have constructed a recombinant vaccinia virus, v-p97NY, which expresses the human melanoma-associated glycoprotein p97. Immunization with v-p97NY could induce humoral and cell-mediated immunity to...

Effect of nonprotective vaccination on antibody response to subsequent human immunodeficiency virus infection.

Pincus, S H, Messer, K G, Hu, S L

We have investigated the systemic anti-HIV antibody response in chimpanzees who were immunized with live vaccinia containing either the HIV envelope glycoprotein (gp160IIIB) or a control antigen...

Protection of macaques against simian AIDS by immunization with a recombinant vaccinia virus expressing the envelope glycoproteins of simian type D retrovirus.

Hu, S L, Zarling, J M, Chinn, J, Travis, B M, Moran, P A, Sias, J, ...

Simian AIDS (SAIDS) is an endemic disease of macaques that shares many characteristics with AIDS in humans. SAIDS is etiologically linked to infection by a type D retrovirus, SAIDS retrovirus (SRV)....

DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2.

Hu, S L, Manley, J L

We have identified a region of the viral genome required for the initiation of transcription in vitro from the major late promoter of adenovirus 2. A fragment of the adenovirus genome containing the...

Nucleotide and deduced amino acid sequence of the bovine adenovirus type 7 proteinase.

Cai, F X, Tang, D, Hu, S L, Weber, J M

Nucleotide and deduced amino acid sequence of the bovine adenovirus type 3 proteinase.

Cai, F X, Bourbonnière, M, Tang, D, Hu, S L, Weber, J M

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Kempe, T, Sundquist, W I, Chow, F, Hu, S L

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to...

The genome of Shope fibroma virus, a tumorigenic poxvirus, contains a growth factor gene with sequence similarity to those encoding epidermal growth factor and transforming growth factor alpha.

Chang, W, Upton, C, Hu, S L, Purchio, A F, McFadden, G

Degenerate oligonucleotide probes corresponding to a highly conserved region common to epidermal growth factor, transforming growth factor alpha, and vaccinia growth factor were used to identify a...

Enhanced immunity to human immunodeficiency virus (HIV) envelope elicited by a combined vaccine regimen consisting of priming with a vaccinia recombinant expressing HIV envelope and boosting with gp160 protein.

Cooney, E L, McElrath, M J, Corey, L, Hu, S L, Collier, A C, Arditti, D, ...

Transmission studies have suggested that an optimal human immunodeficiency virus type 1 (HIV-1) vaccine should induce both neutralizing antibodies and cytolytic T cells to eliminate free virus and...

Sulfhydryl oxidation down-regulates T-cell signaling and inhibits tyrosine phosphorylation of phospholipase C gamma 1.

Kanner, S B, Kavanagh, T J, Grossmann, A, Hu, S L, Bolen, J B, Rabinovitch, P S, ...

Early events in both T-cell receptor (CD3)- and CD4-induced signal transduction pathways include tyrosine phosphorylation of protein substrates, the generation of phosphatidylinositol-phosphate...

Human papillomavirus type 16 nucleoprotein E7 is a tumor rejection antigen.

Chen, L P, Thomas, E K, Hu, S L, Hellström, I, Hellström, K E

It has been speculated that immunological mechanisms play an important role in the control of carcinomas associated with human papillomavirus (HPV), such as cervical cancers. We have now demonstrated...

Detection of simian immunodeficiency virus (SIV)-specific CD8+ T cells in macaques protected from SIV challenge by prior SIV subunit vaccination.

Kent, S J, Hu, S L, Corey, L, Morton, W R, Greenberg, P D

Vaccines for lentiviruses would ideally induce in the host complete resistance to infection of host cells. However, such sterilizing immunity may be neither readily achievable nor absolutely...

Plasma viremia in macaques infected with simian immunodeficiency virus: plasma viral load early in infection predicts survival.

Watson, A, Ranchalis, J, Travis, B, McClure, J, Sutton, W, Johnson, P R, ...

A reliable method for the quantitation of plasma viremia in nonhuman primates infected with simian immunodeficiency virus (SIV) and related viruses is described. This method is based on an...

Inhibition of virus production in peripheral blood mononuclear cells from human immunodeficiency virus (HIV) type 1-seropositive donors by treatment with recombinant HIV-like particles.

Haffar, O K, Moran, P A, Smithgall, M D, Diegel, M L, Sridhar, P, Ledbetter, J A, ...

We have previously reported on the assembly of recombinant human immunodeficiency virus (HIV)-like particles that contain gag structural proteins and present env glycoproteins gp120 and gp41 on their...

Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system.

Haffar, O, Garrigues, J, Travis, B, Moran, P, Zarling, J, Hu, S L

We report the assembly of human immunodeficiency virus (HIV)-like particles in African green monkey kidney cells coinfected with two recombinant vaccinia viruses, one carrying the HIV-1 gag and...

Characterization of a recombinant vaccinia virus expressing human melanoma-associated antigen p97.

Hu, S L, Plowman, G D, Sridhar, P, Stevenson, U S, Brown, J P, Estin, C D

p97 is a cell surface glycoprotein expressed at high levels in most human melanomas but present only in trace amounts in normal adult tissues. We are interested in exploring the possibility of using...

Effects of anti-gp120 monoclonal antibodies on CD4 receptor binding by the env protein of human immunodeficiency virus type 1.

Linsley, P S, Ledbetter, J A, Kinney-Thomas, E, Hu, S L

Monoclonal antibodies (MAbs) to defined peptide epitopes on gp120 from human immunodeficiency virus type 1 were used to investigate the involvement of their epitopes in gp120 binding to the CD4...

Sequence homology between bovine and human adenoviruses.

Hu, S L, Hays, W W, Potts, D E

Cross-hybridization has been detected between corresponding regions of the genomes of bovine adenovirus type 3 and human adenovirus type 2. The most conserved region on the viral genomes encodes the...

Restriction analysis and homology studies of the bovine adenovirus 7 genome.

Hu, S L, Battles, J K, Potts, D E

We located the cleavage sites for restriction endonucleases EcoRI, HindIII, and BamHI on the genome of bovine adenovirus 7. Cross-hybridization at reduced stringency revealed two regions of homology...

Recombinant vaccinia virus vaccine against the human melanoma antigen p97 for use in immunotherapy.

Estin, C D, Stevenson, U S, Plowman, G D, Hu, S L, Sridhar, P, Hellström, I, ...

We have constructed a recombinant vaccinia virus, v-p97NY, which expresses the human melanoma-associated glycoprotein p97. Immunization with v-p97NY could induce humoral and cell-mediated immunity to...

Effect of nonprotective vaccination on antibody response to subsequent human immunodeficiency virus infection.

Pincus, S H, Messer, K G, Hu, S L

We have investigated the systemic anti-HIV antibody response in chimpanzees who were immunized with live vaccinia containing either the HIV envelope glycoprotein (gp160IIIB) or a control antigen...

Protection of macaques against simian AIDS by immunization with a recombinant vaccinia virus expressing the envelope glycoproteins of simian type D retrovirus.

Hu, S L, Zarling, J M, Chinn, J, Travis, B M, Moran, P A, Sias, J, ...

Simian AIDS (SAIDS) is an endemic disease of macaques that shares many characteristics with AIDS in humans. SAIDS is etiologically linked to infection by a type D retrovirus, SAIDS retrovirus (SRV)....

DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2.

Hu, S L, Manley, J L

We have identified a region of the viral genome required for the initiation of transcription in vitro from the major late promoter of adenovirus 2. A fragment of the adenovirus genome containing the...

Nucleotide and deduced amino acid sequence of the bovine adenovirus type 7 proteinase.

Cai, F X, Tang, D, Hu, S L, Weber, J M

Nucleotide and deduced amino acid sequence of the bovine adenovirus type 3 proteinase.

Cai, F X, Bourbonnière, M, Tang, D, Hu, S L, Weber, J M

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Kempe, T, Sundquist, W I, Chow, F, Hu, S L

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to...

The genome of Shope fibroma virus, a tumorigenic poxvirus, contains a growth factor gene with sequence similarity to those encoding epidermal growth factor and transforming growth factor alpha.

Chang, W, Upton, C, Hu, S L, Purchio, A F, McFadden, G

Degenerate oligonucleotide probes corresponding to a highly conserved region common to epidermal growth factor, transforming growth factor alpha, and vaccinia growth factor were used to identify a...