Cornick, N. A., Cuchural, G. J., Snydman, D. R., Jacobus, N. V., Iannini, P., Hill, G., ...
A nationwide survey to monitor tbe susceptibility of the Bacteroides fragilis group, which began in 1981, was continued during 1987. In addition to the eleven drugs evaluated in 1986, sulbactam, a...
In-vitro activity of Sch 34343 against anaerobic bacteria (1985)
Wexler, H., Carter, W. T., Finegold, S. M.
The in-vitro activity of Sch 34343, was compared with that of imipenem against 226 strains of anaerobic bacteria. Sch 34343 was identical in activity to imipenem, inhibiting 99.5% of the organisms...
The in-vitro activity of ceftizoxime against anaerobic bacteria, especially Bacteroides fragilis, is reviewed, based on our own experience as well as on other sources. In-vitro data on ceftizoxime...
Rapid {beta}-lactamase testing in bacteroides (1981)
Gabay, E. L., Sutter, V. L., Finegold, S. M.
Ninety-six strains of bacteroides were tested for the presence of β-lactamase activity using the chromogenic cephalosporin nitrocefin, an iodometric technique for the detection of penicillinase, and...
Cefoxitin sodium therapy of anaerobic infections (1978)
Busch, D. F., Heseltine, P. N. R., Meyer, R. D., Sutter, V. L., Finegold, S. M.
Fifteen in-patients with a total of 17 infections involving anaerobic bacteria were treated with cefoxitin i.v., 4 to 12 g per day. Seven airway-related infections yielded 3-6 anaerobic isolates per...
Susceptibility of anaerobes to cefoxitin sodium and cephalothin (1978)
Sutter, V. L., Oberhammer, L, Finegold, S. M.
The susceptibility in vitro of 295 strains of anaerobic bacteria to cefoxitin sodium and cephalothin was determined by use of an agar-dilution technique. The strains were isolated from clinical...
In vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.
Wexler, H M, Molitoris, E, Molitoris, D, Finegold, S M
The antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the National Committee for Clinical Laboratory Standards-approved Wadsworth agar dilution...
Cell-wall-defective variants of Fusobacterium.
Johnson, C C, Wexler, H M, Becker, S, Garcia, M, Finegold, S M
The activity of antimicrobial agents against Fusobacterium species has been reported as variable in the literature. For some strains, the inconsistency arises from difficulty in determining the...
Aldridge, K E, Wexler, H M, Sanders, C V, Finegold, S M
With 120 clinical isolates of the Bacteroides fragilis group, a comparison of rates of resistance to selected antimicrobial agents by using two susceptibility tests was performed in two medical...
Cuchural, G J, Tally, F P, Jacobus, N V, Cleary, T, Finegold, S M, Hill, G, ...
A nationwide susceptibility survey of 557 isolates of the Bacteroides fragilis group was continued in 1986. The most active beta-lactam drugs were imipenem and ticarcillin-clavulanic acid, which had...
Wexler, H M, Lavin, P T, Molitoris, E, Finegold, S M
A pilot study was designed to estimate the variance components in the determination of the MIC of cefoxitin for isolates of the Bacteroides fragilis group. Twenty different organisms were tested, and...
The activity of two cefoperazone-sulbactam combinations against anaerobic bacteria was tested and compared both with that of cefoperazone alone and with that of other commonly used antimicrobial...
The activity of cefotetan against 430 strains of anaerobic bacteria was compared with that of cefoxitin, ceftizoxime, clindamycin, metronidazole, and chloramphenicol. Percent susceptible values for...
Cuchural, G J, Tally, F P, Jacobus, N V, Aldridge, K, Cleary, T, Finegold, S M, ...
An ongoing survey of the susceptibility of the Bacteroides fragilis group of bacteria was continued at New England Medical Center in 1984 and 1985. A total of 1,229 strains were obtained from eight...
Bowel flora changes in humans receiving cefixime (CL 284,635) or cefaclor.
Finegold, S M, Ingram-Drake, L, Gee, R, Reinhardt, J, Edelstein, M A, MacDonald, K, ...
Twelve healthy young male subjects received either cefixime, a new oral cephalosporin (CL 284,635), or cefaclor (six subjects on each drug) orally for 2 weeks. In the case of cefixime, single daily...
Mulligan, M E, Citron, D, Gabay, E, Kirby, B D, George, W L, Finegold, S M
To evaluate the effects of parenteral cefoxitin therapy on human fecal flora, we cultured fecal specimens obtained from six patients before, during, and after therapy and used standard methods to...
Nationwide study of the susceptibility of the Bacteroides fragilis group in the United States.
Tally, F P, Cuchural, G J, Jacobus, N V, Gorbach, S L, Aldridge, K, Cleary, T, ...
A nationwide susceptibility survey of the Bacteroides fragilis group was continued at New England Medical Center in 1983. A total of 555 strains were obtained from eight centers in the United States....
In vitro activity of cefbuperazone against anaerobic bacteria.
Wexler, H, Carter, W T, Harris, B H, Finegold, S M
The in vitro activity of cefbuperazone was compared with that of cefoxitin, moxalactam, and piperacillin against 305 strains of anaerobic bacteria. Piperacillin was the most active overall,...
In vitro efficacy of sulbactam combined with ampicillin against anaerobic bacteria.
Wexler, H M, Harris, B, Carter, W T, Finegold, S M
An ampicillin-sulbactam combination was compared with ampicillin alone, chloramphenicol, clindamycin, and metronidazole against 272 strains of anaerobic bacteria. Chloramphenicol and...
Goldstein, E J, Citron, D M, Vagvolgyi, A E, Finegold, S M
The susceptibility of 93 aerobic and 59 anaerobic bacteria isolated from human and animal bite wounds was determined by agar dilution. No agent tested (penicillin, oxacillin, cephalexin,...
Effect of carbon dioxide on in vitro susceptibility of anaerobic bacteria to erythromycin.
Goldstein, E J, Sutter, V L, Kwok, Y Y, Lewis, R P, Finegold, S M
The activity of erythromycin against 317 strains of anaerobic bacteria, including 133 strains of the Bacteroides fragilis group, was tested by the agar dilution method in an anaerobic atmosphere with...
Intravenous metronidazole for treatment of infections involving anaerobic bacteria.
George, W L, Kirby, B D, Sutter, V L, Wheeler, L A, Mulligan, M E, Finegold, S M
Intravenous metronidazole was administered, either by continuous or intermittent infusion, to 20 patients with infections involving anaerobic bacteria; 14 of the 20 patients were changed to oral...
Clinical evaluation of moxalactam.
Mathisen, G E, Meyer, R D, Thompson, J M, Finegold, S M
Moxalactam was administered intravenously or intramuscularly or both in doses of 1 to 12 g/day to 45 patients with clinically significant infections (17 soft tissue or bone, 9 pleuropulmonary, 9...
Impact of cefoperazone therapy on fecal flora.
Mulligan, M E, Citron, D M, McNamara, B T, Finegold, S M
To evaluate the effects of parenteral cefoperazone therapy upon human fecal flora, fecal specimens obtained from four patients before and during therapy (as well as after therapy for one patient)...
Comparative in vitro activity of ceftriaxone against anaerobic bacteria.
The in vitro activity of ceftriaxone was compared with those of other recently introduced beta-lactam antimicrobial agents (cefoperazone, cefotaxime, and moxalactam) and with those of cefoxitin,...
Susceptibility of the Bacteroides fragilis group in the United States in 1981.
Tally, F P, Cuchural, G J, Jacobus, N V, Gorbach, S L, Aldridge, K E, Cleary, T J, ...
The minimal inhibitory concentrations of nine antimicrobial agents was determined for over 750 clinical isolates of the Bacteroides fragilis group of anaerobic bacteria collected from nine centers in...
The in vitro activities of cefmenoxime, cefotetan, and N-formimidoyl thienamycin were compared with those of other antimicrobial agents (metronidazole, clindamycin, cefoxitin, and moxalactam) against...
Comparative in vitro activities of cefpiramide and apalcillin against anaerobic bacteria.
Wexler, H, Carter, W T, Harris, B, Finegold, S M
The in vitro activities of two new antimicrobial agents, apalcillin and cefpiramide (SM-1652), were evaluated against 324 strains of anaerobic bacteria. Apalcillin (a penicillin derivative) and...
In vitro activities of three of the newer quinolones against anaerobic bacteria.
Wexler, H M, Molitoris, E, Finegold, S M
The antimicrobial activities of three new quinolone compounds, sparfloxacin, temafloxacin, and WIN 57273, against anaerobic bacteria were determined in three separate studies. The Wadsworth agar...
Summanen, P, Wexler, H M, Finegold, S M
Initial susceptibility studies of Bilophila wadsworthia indicated significant resistance to several beta-lactam antibiotics, including imipenem and cefoxitin. NO beta-lactamase production was...
In vitro activity of Bay Y3118 against anaerobic bacteria.
Wexler, H M, Molitoris, E, Finegold, S M
The antimicrobial activity of a new quinolone, Bay Y3118, was determined against 326 strains of anaerobic bacteria and compared with the activities of ampicillin-sulbactam, cefotetan, clindamycin,...
Summanen, P, Wexler, H M, Lee, K, Becker, S A, Garcia, M M, Finegold, S M
The penicillin-binding protein (PBP) patterns of six strains of Bilophila wadsworthia were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and subsequent...
Wexler, H M, Molitoris, E, Murray, P R, Washington, J, Zabransky, R J, Edelstein, P H, ...
A multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (SGE) method for the determination of the antimicrobial susceptibilities of anaerobes and...
New markers for Eubacterium lentum.
Bokkenheuser, V D, Winter, J, Finegold, S M, Sutter, V L, Ritchie, A E, Moore, W E, ...
Of 37 strains of Eubacterium lentum and phenotypically similar organisms, 26 (70%) synthesized a corticoid 21-dehydroxylase and/or a 3 alpha-hydroxysteroid dehydrogenase. It appeared that the...
UV red fluorescence of Eubacterium lentum.
Mosca, A, Strong, C A, Finegold, S M
Twenty-nine clinical isolates of Eubacterium lentum and two type species were evaluated for the ability to fluoresce under UV light. Twenty-one of the 29 isolates and both of the reference strains...
Preliminary study using species-specific oligonucleotide probe for rRNA of Bilophila wadsworthia.
Sapico, F L, Reeves, D, Wexler, H M, Duncan, J, Wilson, K H, Finegold, S M
Portions of the 16S RNA from a urease-positive Bilophila wadsworthia strain were sequenced, and a probe was constructed. The probe was end labeled with [32P]ATP and polynucleotide kinase and...
Bilophila wadsworthia isolates from clinical specimens.
Baron, E J, Curren, M, Henderson, G, Jousimies-Somer, H, Lee, K, Lechowitz, K, ...
Bilophila wadsworthia is an anaerobic, gram-negative, asaccharolytic, bile-resistant, catalase-positive bacillus that is usually urease positive and was originally recognized in specimens of...
Bacteriologic and clinical study of Bacteroides oris and Bacteroides buccae.
Johnston, B L, Edelstein, M A, Holloway, E Y, Finegold, S M
We characterized clinical isolates previously identified in our laboratory as Bacteroides ruminicola, the human strains of which are now classified as Bacteroides oris and Bacteroides buccae. A total...
Selective medium for isolation of Bacteroides gracilis.
Lee, K, Baron, E J, Summanen, P, Finegold, S M
A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium...
Citron, D M, Baron, E J, Finegold, S M, Goldstein, E J
The rapid identification of isolates of bile-resistant Bacteroides species has clinical and therapeutic relevance because of differences in their patterns of susceptibility and virulence. Five...
Bacteroides gracilis, an important anaerobic bacterial pathogen.
Johnson, C C, Reinhardt, J F, Edelstein, M A, Mulligan, M E, George, W L, Finegold, S M
Clinical isolates of agar-pitting, formate-fumarate-requiring, anaerobic gram-negative bacilli were recharacterized in consideration of the species descriptions of Bacteroides ureolyticus and the...
Cellular fatty acids in Fusobacterium species as a tool for identification.
Tunér, K, Baron, E J, Summanen, P, Finegold, S M
Identification of fusobacteria from clinical specimens currently requires analysis of metabolic end products by gas-liquid chromatography in addition to certain biochemical and enzymatic tests...
Antigenic distinctiveness of Mobiluncus curtisii and Mobiluncus mulieris.
Roberts, M C, Baron, E J, Finegold, S M, Kenny, G E
A total of 26 Mobiluncus strains (17 M. curtisii and 9 M. mulieris strains) were compared serologically by double immunodiffusion and immunoblotting against antisera prepared against representative...
George, W L, Rolfe, R D, Finegold, S M
Fecal specimens from 223 subjects were evaluated for the presence of Clostridium difficile by use of a selective medium developed in our laboratory and for the presence of C. difficile cytotoxin. C....
Edelstein, P H, Snitzer, J B, Finegold, S M
Eighty-five potable water samples were analyzed for the presence of Legionella pneumophila by direct plating and by guinea pig inoculation methods. Direct plating methods utilized...
Selective and differential medium for isolation of Clostridium difficile.
George, W L, Sutter, V L, Citron, D, Finegold, S M
Clostridium difficile is a recognized cause of pseudomembranous (antimicrobial agent-associated) colitis and may be one of the causes of antimicrobial agent-induced diarrhea. A selective and...
Isolation of Legionella pneumophila from a transtracheal aspirate.
Isolation of Legionella pneumophila from a transtracheal aspirate was achieved by using simple in vitro culture methods. Clinical microbiologists should routinely culture for this organism from...
Rapid presumptive bacteriological diagnosis of Legionnaires disease.
Faine, S, Edelstein, P, Kirby, B D, Finegold, S M
A simple, relatively rapid silver impregnation stain has been found to stain Legionella pneumophila effectively in paraffin-embedded tissue sections while permitting visualization of histological...
Use of a semiselective medium to culture Legionella pneumophila from contaminated lung specimens.
Legionella pneumophila was successfully isolated, using a semiselective medium, from two of three lung specimens heavily contaminated with other organisms. This medium is composed of charcoal yeast...
Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria.
Halebian, S, Harris, B, Finegold, S M, Rolfe, R D
Several species of anaerobic bacteria display variable Gram stain reactions which often make identification difficult. A simple, rapid method utilizing a 3% solution of potassium hydroxide to...
New medium selective for Fusobacterium species and differential for Fusobacterium necrophorum.
Morgenstein, A A, Citron, D M, Finegold, S M
Fusobacterium egg yolk agar is a new medium selective for Fusobacterium species and differential for Fusobacterium necrophorum. The medium is a Brucella Agar base (Difco Laboratories, Detroit, Mich.)...
Comparison of liquid growth media for Legionella pneumophila.
Saito, A, Rolfe, R D, Edelstein, P H, Finegold, S M
Ten liquid media were compared under standard conditions for their ability to support the growth of Legionella pneumophila. Modified gonococcal-ferric cysteine broth (without sodium chloride)...
Wideman, P A, Vargo, V L, Citronbaum, D, Finegold, S M
The previously reported sodium polyanethol sulfonate disk test for the identification of Peptostreptococcus anaerobius (Graves et al., 1974) was evaluated, with modifications. Three bands of brucella...
Characterization of bacteroides melaninogenicus.
Harding, G K, Sutter, V L, Finegold, S M, Bricknell, K S
Fifty-eight human isolates of Bacteroides melaninogenicus, 42 from a variety of clinical infections and the rest from normal flora, were studied for pigment production and ultraviolet light...
Comparison of three procedures for biochemical testing of anaerobic bacteria.
Moore, H B, Sutter, V L, Finegold, S M
The Analytab Products, Inc. (API), anaerobic multitest microsystem (MICRO) was compared with the Center for Disease Control conventional (CONV) thioglycolate (supplemented with hemin and vitamin K1)...
Practical aerobic membrane filtration blood culture technique: development of procedure.
Sullivan, N M, Sutter, V L, Finegold, S M
The advantages of a membrane filter system for blood culturing have been realized for many years. Lysing of the blood prior to filtration is a convenient way to proceed, but previously described...
Practical aerobic membrane filtration blood culture technique: clinical blood culture trial.
Sullivan, N M, Sutter, V L, Finegold, S M
During the course of preliminary clinical trials of an improved membrane filter blood culture system, filter plugging produced by a gelling of the lysing solution was observed when the patients had...
Bacteriology of human and animal bite wounds.
Goldstein, E J, Citron, D M, Wield, B, Blachman, U, Sutter, V L, Miller, T A, ...
Seventy-three patients with bite wounds (16 patients with clenched-fist injuries, 18 with human bite wounds, and 39 with animal bites) were cultured aerobically and anaerobically. A total of 33 of 34...
Cuchural, G J, Tally, F P, Jacobus, N V, Gorbach, S L, Aldridge, K, Cleary, T, ...
A susceptibility survey of 537 strains of the Bacteroides fragilis group from eight centers in the United States was continued at the Tufts New England Medical Center in 1982. The results were...
Wexler, H M, Molitoris, E, Jashnian, F, Finegold, S M
Antimicrobial susceptibility tests were performed on brucella laked blood agar with 340 isolates and 14 antimicrobial agents by the standard agar dilution technique and the spiral gradient technique...
Wexler, H M, Molitoris, E, Finegold, S M
The in vitro activities of several new beta-lactam-beta-lactamase inhibitor combinations (piperacillin plus tazobactam, ceftizoxime and cefonicid with sulbactam and clavulanic acid, and ampicillin...
In vitro activity of DU-6859a against anaerobic bacteria.
Wexler, H M, Molitoris, E, Reeves, D, Finegold, S M
The activity of a new quinolone agent, DU-6859a, against 330 strains of anaerobic bacteria was determined by using the National Committee for Clinical Laboratory Standards-approved Wadsworth brucella...
Wexler, H M, Molitoris, E, Finegold, S M
The in vitro activities of the N,N-dimethylglycylamido derivatives of minocycline (DMG-MINO) and 6-demethyl-6-deoxytetracycline (DMG-DMDOT) were compared with those of minocycline, tetracycline,...
Vesely, D L, Straub, K D, Nolan, C M, Rolfe, R D, Finegold, S M, Monson, T P
Antibiotic-associated pseudomembranous colitis has been linked with Clostridium difficile toxin. We examined the effect of toxins from four strains of C. difficile isolated from patients with...
Attebery, H. R., Finegold, S. M.
A double-closured method introduced for Hungate tubes eliminates clamps or special racks to hold the rubber stoppers in place while the tubes are being processed. The system prevents stoppers from...
Sullivan, N. M., Sutter, V. L., Carter, W. T., Attebery, H. R., Finegold, S. M.
A total of 300 patients undergoing various types of urological procedures was studied for incidence of bacteremia. An osmotically stabilized anaerobic broth with sodium polyanethol sulfonate...
Purification and characterization of Clostridium difficile toxin.
Recent evidence indicates that toxigenic Clostridium difficile strains are a major cause of antimicrobial-associated ileocecitis in laboratory animals and pseudomembranous colitis in humans. C....
Song, Y. L., Liu, C. X., McTeague, M., Finegold, S. M.
Two groups of unknown bacteria, which phenotypically resemble members of the Bacteroides fragilis group but phylogenetically display >5% 16S rRNA gene sequence divergence from their nearest validly...
Ultrastructure and molecular characterization of Fusobacterium necrophorum biovars.
Garcia, M M, Becker, S A, Brooks, B W, Berg, J N, Finegold, S M
The ultrastructural features and molecular components of 18 strains of Fusobacterium necrophorum biovars A, AB and B, isolated from animal and human infections, were examined by electron microscopy,...
The bacteriology of gangrenous and perforated appendicitis--revisited.
Bennion, R S, Baron, E J, Thompson, J E, Downes, J, Summanen, P, Talan, D A, ...
By using optimum sampling, transport, and culture techniques in patients with gangrenous or perforated appendicitis, we recovered than has previously been reported. Thirty patients older than 12...
In Vitro Activities of DX-619 and Four Comparator Agents against 376 Anaerobic Bacterial Isolates
Molitoris, D., Bolaños, M., Finegold, S. M.
The activity of DX-619 was evaluated against 376 anaerobic isolates using the reference CLSI agar dilution method. Overall, 90% of the strains were susceptible to DX-619 at ≤1 μg/ml. It was more...
Pyogenic liver abscess due to microaerophilic streptococci.
Patterson, D K, Ozeran, R S, Glantz, G J, Miller, A B, Finegold, S M
In vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.
Wexler, H M, Molitoris, E, Molitoris, D, Finegold, S M
The antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the National Committee for Clinical Laboratory Standards-approved Wadsworth agar dilution...
Cell-wall-defective variants of Fusobacterium.
Johnson, C C, Wexler, H M, Becker, S, Garcia, M, Finegold, S M
The activity of antimicrobial agents against Fusobacterium species has been reported as variable in the literature. For some strains, the inconsistency arises from difficulty in determining the...
Aldridge, K E, Wexler, H M, Sanders, C V, Finegold, S M
With 120 clinical isolates of the Bacteroides fragilis group, a comparison of rates of resistance to selected antimicrobial agents by using two susceptibility tests was performed in two medical...
Cuchural, G J, Tally, F P, Jacobus, N V, Cleary, T, Finegold, S M, Hill, G, ...
A nationwide susceptibility survey of 557 isolates of the Bacteroides fragilis group was continued in 1986. The most active beta-lactam drugs were imipenem and ticarcillin-clavulanic acid, which had...
Wexler, H M, Lavin, P T, Molitoris, E, Finegold, S M
A pilot study was designed to estimate the variance components in the determination of the MIC of cefoxitin for isolates of the Bacteroides fragilis group. Twenty different organisms were tested, and...
The activity of two cefoperazone-sulbactam combinations against anaerobic bacteria was tested and compared both with that of cefoperazone alone and with that of other commonly used antimicrobial...
The activity of cefotetan against 430 strains of anaerobic bacteria was compared with that of cefoxitin, ceftizoxime, clindamycin, metronidazole, and chloramphenicol. Percent susceptible values for...
Cuchural, G J, Tally, F P, Jacobus, N V, Aldridge, K, Cleary, T, Finegold, S M, ...
An ongoing survey of the susceptibility of the Bacteroides fragilis group of bacteria was continued at New England Medical Center in 1984 and 1985. A total of 1,229 strains were obtained from eight...
Bowel flora changes in humans receiving cefixime (CL 284,635) or cefaclor.
Finegold, S M, Ingram-Drake, L, Gee, R, Reinhardt, J, Edelstein, M A, MacDonald, K, ...
Twelve healthy young male subjects received either cefixime, a new oral cephalosporin (CL 284,635), or cefaclor (six subjects on each drug) orally for 2 weeks. In the case of cefixime, single daily...
Mulligan, M E, Citron, D, Gabay, E, Kirby, B D, George, W L, Finegold, S M
To evaluate the effects of parenteral cefoxitin therapy on human fecal flora, we cultured fecal specimens obtained from six patients before, during, and after therapy and used standard methods to...
Nationwide study of the susceptibility of the Bacteroides fragilis group in the United States.
Tally, F P, Cuchural, G J, Jacobus, N V, Gorbach, S L, Aldridge, K, Cleary, T, ...
A nationwide susceptibility survey of the Bacteroides fragilis group was continued at New England Medical Center in 1983. A total of 555 strains were obtained from eight centers in the United States....
In vitro activity of cefbuperazone against anaerobic bacteria.
Wexler, H, Carter, W T, Harris, B H, Finegold, S M
The in vitro activity of cefbuperazone was compared with that of cefoxitin, moxalactam, and piperacillin against 305 strains of anaerobic bacteria. Piperacillin was the most active overall,...
In vitro efficacy of sulbactam combined with ampicillin against anaerobic bacteria.
Wexler, H M, Harris, B, Carter, W T, Finegold, S M
An ampicillin-sulbactam combination was compared with ampicillin alone, chloramphenicol, clindamycin, and metronidazole against 272 strains of anaerobic bacteria. Chloramphenicol and...
Goldstein, E J, Citron, D M, Vagvolgyi, A E, Finegold, S M
The susceptibility of 93 aerobic and 59 anaerobic bacteria isolated from human and animal bite wounds was determined by agar dilution. No agent tested (penicillin, oxacillin, cephalexin,...
Effect of carbon dioxide on in vitro susceptibility of anaerobic bacteria to erythromycin.
Goldstein, E J, Sutter, V L, Kwok, Y Y, Lewis, R P, Finegold, S M
The activity of erythromycin against 317 strains of anaerobic bacteria, including 133 strains of the Bacteroides fragilis group, was tested by the agar dilution method in an anaerobic atmosphere with...
Intravenous metronidazole for treatment of infections involving anaerobic bacteria.
George, W L, Kirby, B D, Sutter, V L, Wheeler, L A, Mulligan, M E, Finegold, S M
Intravenous metronidazole was administered, either by continuous or intermittent infusion, to 20 patients with infections involving anaerobic bacteria; 14 of the 20 patients were changed to oral...
Clinical evaluation of moxalactam.
Mathisen, G E, Meyer, R D, Thompson, J M, Finegold, S M
Moxalactam was administered intravenously or intramuscularly or both in doses of 1 to 12 g/day to 45 patients with clinically significant infections (17 soft tissue or bone, 9 pleuropulmonary, 9...
Impact of cefoperazone therapy on fecal flora.
Mulligan, M E, Citron, D M, McNamara, B T, Finegold, S M
To evaluate the effects of parenteral cefoperazone therapy upon human fecal flora, fecal specimens obtained from four patients before and during therapy (as well as after therapy for one patient)...
Comparative in vitro activity of ceftriaxone against anaerobic bacteria.
The in vitro activity of ceftriaxone was compared with those of other recently introduced beta-lactam antimicrobial agents (cefoperazone, cefotaxime, and moxalactam) and with those of cefoxitin,...
Susceptibility of the Bacteroides fragilis group in the United States in 1981.
Tally, F P, Cuchural, G J, Jacobus, N V, Gorbach, S L, Aldridge, K E, Cleary, T J, ...
The minimal inhibitory concentrations of nine antimicrobial agents was determined for over 750 clinical isolates of the Bacteroides fragilis group of anaerobic bacteria collected from nine centers in...
The in vitro activities of cefmenoxime, cefotetan, and N-formimidoyl thienamycin were compared with those of other antimicrobial agents (metronidazole, clindamycin, cefoxitin, and moxalactam) against...
Comparative in vitro activities of cefpiramide and apalcillin against anaerobic bacteria.
Wexler, H, Carter, W T, Harris, B, Finegold, S M
The in vitro activities of two new antimicrobial agents, apalcillin and cefpiramide (SM-1652), were evaluated against 324 strains of anaerobic bacteria. Apalcillin (a penicillin derivative) and...
In vitro activities of three of the newer quinolones against anaerobic bacteria.
Wexler, H M, Molitoris, E, Finegold, S M
The antimicrobial activities of three new quinolone compounds, sparfloxacin, temafloxacin, and WIN 57273, against anaerobic bacteria were determined in three separate studies. The Wadsworth agar...
Summanen, P, Wexler, H M, Finegold, S M
Initial susceptibility studies of Bilophila wadsworthia indicated significant resistance to several beta-lactam antibiotics, including imipenem and cefoxitin. NO beta-lactamase production was...
In vitro activity of Bay Y3118 against anaerobic bacteria.
Wexler, H M, Molitoris, E, Finegold, S M
The antimicrobial activity of a new quinolone, Bay Y3118, was determined against 326 strains of anaerobic bacteria and compared with the activities of ampicillin-sulbactam, cefotetan, clindamycin,...
Summanen, P, Wexler, H M, Lee, K, Becker, S A, Garcia, M M, Finegold, S M
The penicillin-binding protein (PBP) patterns of six strains of Bilophila wadsworthia were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and subsequent...
Wexler, H M, Molitoris, E, Murray, P R, Washington, J, Zabransky, R J, Edelstein, P H, ...
A multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (SGE) method for the determination of the antimicrobial susceptibilities of anaerobes and...
New markers for Eubacterium lentum.
Bokkenheuser, V D, Winter, J, Finegold, S M, Sutter, V L, Ritchie, A E, Moore, W E, ...
Of 37 strains of Eubacterium lentum and phenotypically similar organisms, 26 (70%) synthesized a corticoid 21-dehydroxylase and/or a 3 alpha-hydroxysteroid dehydrogenase. It appeared that the...
UV red fluorescence of Eubacterium lentum.
Mosca, A, Strong, C A, Finegold, S M
Twenty-nine clinical isolates of Eubacterium lentum and two type species were evaluated for the ability to fluoresce under UV light. Twenty-one of the 29 isolates and both of the reference strains...
Preliminary study using species-specific oligonucleotide probe for rRNA of Bilophila wadsworthia.
Sapico, F L, Reeves, D, Wexler, H M, Duncan, J, Wilson, K H, Finegold, S M
Portions of the 16S RNA from a urease-positive Bilophila wadsworthia strain were sequenced, and a probe was constructed. The probe was end labeled with [32P]ATP and polynucleotide kinase and...
Bilophila wadsworthia isolates from clinical specimens.
Baron, E J, Curren, M, Henderson, G, Jousimies-Somer, H, Lee, K, Lechowitz, K, ...
Bilophila wadsworthia is an anaerobic, gram-negative, asaccharolytic, bile-resistant, catalase-positive bacillus that is usually urease positive and was originally recognized in specimens of...
Bacteriologic and clinical study of Bacteroides oris and Bacteroides buccae.
Johnston, B L, Edelstein, M A, Holloway, E Y, Finegold, S M
We characterized clinical isolates previously identified in our laboratory as Bacteroides ruminicola, the human strains of which are now classified as Bacteroides oris and Bacteroides buccae. A total...
Selective medium for isolation of Bacteroides gracilis.
Lee, K, Baron, E J, Summanen, P, Finegold, S M
A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium...
Citron, D M, Baron, E J, Finegold, S M, Goldstein, E J
The rapid identification of isolates of bile-resistant Bacteroides species has clinical and therapeutic relevance because of differences in their patterns of susceptibility and virulence. Five...
Bacteroides gracilis, an important anaerobic bacterial pathogen.
Johnson, C C, Reinhardt, J F, Edelstein, M A, Mulligan, M E, George, W L, Finegold, S M
Clinical isolates of agar-pitting, formate-fumarate-requiring, anaerobic gram-negative bacilli were recharacterized in consideration of the species descriptions of Bacteroides ureolyticus and the...
Cellular fatty acids in Fusobacterium species as a tool for identification.
Tunér, K, Baron, E J, Summanen, P, Finegold, S M
Identification of fusobacteria from clinical specimens currently requires analysis of metabolic end products by gas-liquid chromatography in addition to certain biochemical and enzymatic tests...
Antigenic distinctiveness of Mobiluncus curtisii and Mobiluncus mulieris.
Roberts, M C, Baron, E J, Finegold, S M, Kenny, G E
A total of 26 Mobiluncus strains (17 M. curtisii and 9 M. mulieris strains) were compared serologically by double immunodiffusion and immunoblotting against antisera prepared against representative...
George, W L, Rolfe, R D, Finegold, S M
Fecal specimens from 223 subjects were evaluated for the presence of Clostridium difficile by use of a selective medium developed in our laboratory and for the presence of C. difficile cytotoxin. C....
Edelstein, P H, Snitzer, J B, Finegold, S M
Eighty-five potable water samples were analyzed for the presence of Legionella pneumophila by direct plating and by guinea pig inoculation methods. Direct plating methods utilized...
Selective and differential medium for isolation of Clostridium difficile.
George, W L, Sutter, V L, Citron, D, Finegold, S M
Clostridium difficile is a recognized cause of pseudomembranous (antimicrobial agent-associated) colitis and may be one of the causes of antimicrobial agent-induced diarrhea. A selective and...
Isolation of Legionella pneumophila from a transtracheal aspirate.
Isolation of Legionella pneumophila from a transtracheal aspirate was achieved by using simple in vitro culture methods. Clinical microbiologists should routinely culture for this organism from...
Rapid presumptive bacteriological diagnosis of Legionnaires disease.
Faine, S, Edelstein, P, Kirby, B D, Finegold, S M
A simple, relatively rapid silver impregnation stain has been found to stain Legionella pneumophila effectively in paraffin-embedded tissue sections while permitting visualization of histological...
Use of a semiselective medium to culture Legionella pneumophila from contaminated lung specimens.
Legionella pneumophila was successfully isolated, using a semiselective medium, from two of three lung specimens heavily contaminated with other organisms. This medium is composed of charcoal yeast...
Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria.
Halebian, S, Harris, B, Finegold, S M, Rolfe, R D
Several species of anaerobic bacteria display variable Gram stain reactions which often make identification difficult. A simple, rapid method utilizing a 3% solution of potassium hydroxide to...
New medium selective for Fusobacterium species and differential for Fusobacterium necrophorum.
Morgenstein, A A, Citron, D M, Finegold, S M
Fusobacterium egg yolk agar is a new medium selective for Fusobacterium species and differential for Fusobacterium necrophorum. The medium is a Brucella Agar base (Difco Laboratories, Detroit, Mich.)...
Comparison of liquid growth media for Legionella pneumophila.
Saito, A, Rolfe, R D, Edelstein, P H, Finegold, S M
Ten liquid media were compared under standard conditions for their ability to support the growth of Legionella pneumophila. Modified gonococcal-ferric cysteine broth (without sodium chloride)...
Wideman, P A, Vargo, V L, Citronbaum, D, Finegold, S M
The previously reported sodium polyanethol sulfonate disk test for the identification of Peptostreptococcus anaerobius (Graves et al., 1974) was evaluated, with modifications. Three bands of brucella...
Characterization of bacteroides melaninogenicus.
Harding, G K, Sutter, V L, Finegold, S M, Bricknell, K S
Fifty-eight human isolates of Bacteroides melaninogenicus, 42 from a variety of clinical infections and the rest from normal flora, were studied for pigment production and ultraviolet light...
Comparison of three procedures for biochemical testing of anaerobic bacteria.
Moore, H B, Sutter, V L, Finegold, S M
The Analytab Products, Inc. (API), anaerobic multitest microsystem (MICRO) was compared with the Center for Disease Control conventional (CONV) thioglycolate (supplemented with hemin and vitamin K1)...
Practical aerobic membrane filtration blood culture technique: development of procedure.
Sullivan, N M, Sutter, V L, Finegold, S M
The advantages of a membrane filter system for blood culturing have been realized for many years. Lysing of the blood prior to filtration is a convenient way to proceed, but previously described...
Practical aerobic membrane filtration blood culture technique: clinical blood culture trial.
Sullivan, N M, Sutter, V L, Finegold, S M
During the course of preliminary clinical trials of an improved membrane filter blood culture system, filter plugging produced by a gelling of the lysing solution was observed when the patients had...
Bacteriology of human and animal bite wounds.
Goldstein, E J, Citron, D M, Wield, B, Blachman, U, Sutter, V L, Miller, T A, ...
Seventy-three patients with bite wounds (16 patients with clenched-fist injuries, 18 with human bite wounds, and 39 with animal bites) were cultured aerobically and anaerobically. A total of 33 of 34...
Cuchural, G J, Tally, F P, Jacobus, N V, Gorbach, S L, Aldridge, K, Cleary, T, ...
A susceptibility survey of 537 strains of the Bacteroides fragilis group from eight centers in the United States was continued at the Tufts New England Medical Center in 1982. The results were...
Wexler, H M, Molitoris, E, Jashnian, F, Finegold, S M
Antimicrobial susceptibility tests were performed on brucella laked blood agar with 340 isolates and 14 antimicrobial agents by the standard agar dilution technique and the spiral gradient technique...
Wexler, H M, Molitoris, E, Finegold, S M
The in vitro activities of several new beta-lactam-beta-lactamase inhibitor combinations (piperacillin plus tazobactam, ceftizoxime and cefonicid with sulbactam and clavulanic acid, and ampicillin...
In vitro activity of DU-6859a against anaerobic bacteria.
Wexler, H M, Molitoris, E, Reeves, D, Finegold, S M
The activity of a new quinolone agent, DU-6859a, against 330 strains of anaerobic bacteria was determined by using the National Committee for Clinical Laboratory Standards-approved Wadsworth brucella...
Wexler, H M, Molitoris, E, Finegold, S M
The in vitro activities of the N,N-dimethylglycylamido derivatives of minocycline (DMG-MINO) and 6-demethyl-6-deoxytetracycline (DMG-DMDOT) were compared with those of minocycline, tetracycline,...
Vesely, D L, Straub, K D, Nolan, C M, Rolfe, R D, Finegold, S M, Monson, T P
Antibiotic-associated pseudomembranous colitis has been linked with Clostridium difficile toxin. We examined the effect of toxins from four strains of C. difficile isolated from patients with...
Attebery, H. R., Finegold, S. M.
A double-closured method introduced for Hungate tubes eliminates clamps or special racks to hold the rubber stoppers in place while the tubes are being processed. The system prevents stoppers from...
Sullivan, N. M., Sutter, V. L., Carter, W. T., Attebery, H. R., Finegold, S. M.
A total of 300 patients undergoing various types of urological procedures was studied for incidence of bacteremia. An osmotically stabilized anaerobic broth with sodium polyanethol sulfonate...
Purification and characterization of Clostridium difficile toxin.
Recent evidence indicates that toxigenic Clostridium difficile strains are a major cause of antimicrobial-associated ileocecitis in laboratory animals and pseudomembranous colitis in humans. C....
Song, Y. L., Liu, C. X., McTeague, M., Finegold, S. M.
Two groups of unknown bacteria, which phenotypically resemble members of the Bacteroides fragilis group but phylogenetically display >5% 16S rRNA gene sequence divergence from their nearest validly...
Ultrastructure and molecular characterization of Fusobacterium necrophorum biovars.
Garcia, M M, Becker, S A, Brooks, B W, Berg, J N, Finegold, S M
The ultrastructural features and molecular components of 18 strains of Fusobacterium necrophorum biovars A, AB and B, isolated from animal and human infections, were examined by electron microscopy,...
The bacteriology of gangrenous and perforated appendicitis--revisited.
Bennion, R S, Baron, E J, Thompson, J E, Downes, J, Summanen, P, Talan, D A, ...
By using optimum sampling, transport, and culture techniques in patients with gangrenous or perforated appendicitis, we recovered than has previously been reported. Thirty patients older than 12...
In Vitro Activities of DX-619 and Four Comparator Agents against 376 Anaerobic Bacterial Isolates
Molitoris, D., Bolaños, M., Finegold, S. M.
The activity of DX-619 was evaluated against 376 anaerobic isolates using the reference CLSI agar dilution method. Overall, 90% of the strains were susceptible to DX-619 at ≤1 μg/ml. It was more...
Pyogenic liver abscess due to microaerophilic streptococci.
Patterson, D K, Ozeran, R S, Glantz, G J, Miller, A B, Finegold, S M
Brook, I, Reza, M J, Bricknell, K S, Bluestone, R, Finegold, S M
Gas liquid chromatography was performed on synovial fluid of 94 patients with exudative arthritis. A relatively constant pattern of peaks was obtained in all synovial fluid samples. Lactic acid was...
Snydman, D. R., Jacobus, N. V., McDermott, L. A., Ruthazer, R., Golan, Y., Goldstein, E. J. C., ...
The susceptibility trends for the species of the Bacteroides fragilis group against various antibiotics from 1997 to 2004 were determined by using data for 5,225 isolates referred by 10 medical...
Bacteraemia caused by Anaerotruncus colihominis and emended description of the species
Lau, S K P, Woo, P C Y, Woo, G K S, Fung, A M Y, Ngan, A H Y, Song, Y, ...
Finegold, S. M., Molitoris, D.
Rifaximin, ampicillin-sulbactam, neomycin, nitazoxanide, teicoplanin, and vancomycin were tested against 536 strains of anaerobic bacteria. The overall MIC of rifaximin at which 50% of strains were...
Summanen, P. H., Wooton, J., Finegold, S. M.
The terminology and classification of the Anginosus group streptococci has been inconsistent. We tested the utility of 16S rRNA gene and tuf gene sequencing and conventional biochemical tests for the...