Intracellular trafficking of yeast telomerase components (2007)
Teixeira, M. T., Förstemann, K., Gasser, S. M., Lingner, J.
Telomerase uses an internal RNA moiety as template for the synthesis of telomere repeats. In Saccharomyces cerevisiae, the telomerase holoenzyme contains the telomerase reverse transcriptase subunit...
Sage, D., Neumann, F.R., Hediger, F., Gasser, S.M., Unser, M.
We present a new, robust, computational procedure for tracking fluorescent markers in time-lapse microscopy. The algorithm is optimized for finding the time-trajectory of single particles in very...
Thermal reaction of Pt film with 110 GaN epilayer (1999)
Backscattering spectrometry, x-ray diffractometry, and scanning electron microscopy have been used to study the reaction of a thin Pt film with an epilayer of [110] GaN on [110] sapphire upon...
Instability of Amorphous Ru-Si-O Thin Films under Thermal Oxidation (1999)
Gasser, S. M., Ruiz, R., Kolawa, E.
Ternary films about 200 nm thick of composition Ru20Si15O65 have been synthesized by reactive rf magnetron sputtering of a Ru1Si1 target in an argon-oxygen gas. As-deposited, the films are...
Bilaud, T, Koering, C E, Binet-Brasselet, E, Ancelin, K, Pollice, A, Gasser, S M, ...
The yeast TTAGGG binding factor 1 (Tbf1) was identified and cloned through its ability to interact with vertebrate telomeric repeats in vitro. We show here that a sequence of 60 amino acids located...
Vassetzky, N S, Gaden, F, Brun, C, Gasser, S M, Gilson, E
Band shift assays were used to study proteins from the fission yeast that bind double-stranded telomeric repeat sequences. We also examine general DNA binding properties of the telobox domain, which...
The yeast protein encoded by PUB1 binds T-rich single stranded DNA.
Cockell, M, Frutiger, S, Hughes, G J, Gasser, S M
We have characterized binding activities in yeast which recognise the T-rich strand of the yeast ARS consensus element and have purified two of these to homogeneity. One (ACBP-60) is detectable in...
Gilson, E, Müller, T, Sogo, J, Laroche, T, Gasser, S M
Repressor Activator Protein 1 (RAP1) of Saccharomyces cerevisiae is an abundant nuclear protein implicated in telomere length maintenance, transactivation, and in the establishment of silent...
Verdier, J M, Stalder, R, Roberge, M, Amati, B, Sentenac, A, Gasser, S M
We present a reproducible method for the preparation of nuclear extracts from the yeast Saccharomyces cerevisiae that support efficient RNA polymerase B (II)-dependent transcription. Extracts from...
Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps.
Gasser, S M, Ohashi, A, Daum, G, Böhni, P C, Gibson, J, Reid, G A, ...
Cytochrome b2 of yeast is located in the space between the inner and outer mitochondrial membranes whereas cytochrome c1 is bound to the outer face of the mitochondrial inner membrane. Both proteins...
Histone-depleted nuclei maintain sequence-specific interactions with genomic DNA at sites known as scaffold attachment regions (SARs) or matrix attachment regions. We have previously shown that in...
Cooperation at a distance between silencers and proto-silencers at the yeast HML locus.
Boscheron, C, Maillet, L, Marcand, S, Tsai-Pflugfelder, M, Gasser, S M, Gilson, E
Transcriptional repression at the silent yeast mating type loci is achieved through the formation of a particular nucleoprotein complex at specific cis-acting elements called silencers. This complex...
Amati, B, Pick, L, Laroche, T, Gasser, S M
Nuclei isolated from eukaryotic cells can be depleted of histones and most soluble nuclear proteins to isolate a structural framework called the nuclear scaffold. This structure maintains specific...
Cardenas, M E, Dang, Q, Glover, C V, Gasser, S M
The decatenation activity of DNA topoisomerase II is essential for viability as eukaryotic cells traverse mitosis. Phosphorylation has been shown to stimulate topoisomerase II activity in vitro. Here...
The organisation of chromatin loops: characterization of a scaffold attachment site
Previous experiments have identified a 657-bp restriction fragment in the non-transcribed region of the Drosophila histone gene cluster that is specifically associated with the histone-depleted...
Localization of Sir2p: the nucleolus as a compartment for silent information regulators.
Gotta, M, Strahl-Bolsinger, S, Renauld, H, Laroche, T, Kennedy, B K, Grunstein, M, ...
In wild-type budding yeast strains, the proteins encoded by SIR3, SIR4 and RAP1 co-localize with telomeric DNA in a limited number of foci in interphase nuclei. Immunostaining of Sir2p shows that in...
Vassetzky, Y S, Dang, Q, Benedetti, P, Gasser, S M
We present a novel assay for the study of protein-protein interactions involving DNA topoisomerase II. Under various conditions of incubation we observe that topoisomerase II forms complexes at least...
Analysis of Sir2p domains required for rDNA and telomeric silencing in Saccharomyces cerevisiae.
Cockell, M M, Perrod, S, Gasser, S M
Silent information regulator (Sir) 2 is a limiting component of the Sir2/3/4 complex, which represses transcription at subtelomeric and HM loci. Sir2p also acts independently of Sir3p and Sir4p to...
Bilaud, T, Koering, C E, Binet-Brasselet, E, Ancelin, K, Pollice, A, Gasser, S M, ...
The yeast TTAGGG binding factor 1 (Tbf1) was identified and cloned through its ability to interact with vertebrate telomeric repeats in vitro. We show here that a sequence of 60 amino acids located...
Vassetzky, N S, Gaden, F, Brun, C, Gasser, S M, Gilson, E
Band shift assays were used to study proteins from the fission yeast that bind double-stranded telomeric repeat sequences. We also examine general DNA binding properties of the telobox domain, which...
The yeast protein encoded by PUB1 binds T-rich single stranded DNA.
Cockell, M, Frutiger, S, Hughes, G J, Gasser, S M
We have characterized binding activities in yeast which recognise the T-rich strand of the yeast ARS consensus element and have purified two of these to homogeneity. One (ACBP-60) is detectable in...
Gilson, E, Müller, T, Sogo, J, Laroche, T, Gasser, S M
Repressor Activator Protein 1 (RAP1) of Saccharomyces cerevisiae is an abundant nuclear protein implicated in telomere length maintenance, transactivation, and in the establishment of silent...
Verdier, J M, Stalder, R, Roberge, M, Amati, B, Sentenac, A, Gasser, S M
We present a reproducible method for the preparation of nuclear extracts from the yeast Saccharomyces cerevisiae that support efficient RNA polymerase B (II)-dependent transcription. Extracts from...
Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps.
Gasser, S M, Ohashi, A, Daum, G, Böhni, P C, Gibson, J, Reid, G A, ...
Cytochrome b2 of yeast is located in the space between the inner and outer mitochondrial membranes whereas cytochrome c1 is bound to the outer face of the mitochondrial inner membrane. Both proteins...
Vassetzky, Y S, Dang, Q, Benedetti, P, Gasser, S M
We present a novel assay for the study of protein-protein interactions involving DNA topoisomerase II. Under various conditions of incubation we observe that topoisomerase II forms complexes at least...
Histone-depleted nuclei maintain sequence-specific interactions with genomic DNA at sites known as scaffold attachment regions (SARs) or matrix attachment regions. We have previously shown that in...
Cooperation at a distance between silencers and proto-silencers at the yeast HML locus.
Boscheron, C, Maillet, L, Marcand, S, Tsai-Pflugfelder, M, Gasser, S M, Gilson, E
Transcriptional repression at the silent yeast mating type loci is achieved through the formation of a particular nucleoprotein complex at specific cis-acting elements called silencers. This complex...
Amati, B, Pick, L, Laroche, T, Gasser, S M
Nuclei isolated from eukaryotic cells can be depleted of histones and most soluble nuclear proteins to isolate a structural framework called the nuclear scaffold. This structure maintains specific...
Cardenas, M E, Dang, Q, Glover, C V, Gasser, S M
The decatenation activity of DNA topoisomerase II is essential for viability as eukaryotic cells traverse mitosis. Phosphorylation has been shown to stimulate topoisomerase II activity in vitro. Here...
The organisation of chromatin loops: characterization of a scaffold attachment site
Previous experiments have identified a 657-bp restriction fragment in the non-transcribed region of the Drosophila histone gene cluster that is specifically associated with the histone-depleted...
Localization of Sir2p: the nucleolus as a compartment for silent information regulators.
Gotta, M, Strahl-Bolsinger, S, Renauld, H, Laroche, T, Kennedy, B K, Grunstein, M, ...
In wild-type budding yeast strains, the proteins encoded by SIR3, SIR4 and RAP1 co-localize with telomeric DNA in a limited number of foci in interphase nuclei. Immunostaining of Sir2p shows that in...
Analysis of Sir2p domains required for rDNA and telomeric silencing in Saccharomyces cerevisiae.
Cockell, M M, Perrod, S, Gasser, S M
Silent information regulator (Sir) 2 is a limiting component of the Sir2/3/4 complex, which represses transcription at subtelomeric and HM loci. Sir2p also acts independently of Sir3p and Sir4p to...