S. Nakada

Publication List Details

Period

1994 - 1995

Number

23

Co-Authors

The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis.

Nakagawa, Y, Kimura, N, Toyoda, T, Mizumoto, K, Ishihama, A, Oda, K, ...

An established cell line, clone 64, in which the expression of the RNA polymerase PB1 and PA subunit genes and the nucleoprotein (NP) gene but not the PB2 subunit gene of influenza virus can be...

The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.

Nakagawa, Y, Oda, K, Nakada, S

We indicated that the PB1 and PA subunits of RNA polymerase and nucleoprotein (NP) can support replication of the influenza virus genome as well as transcription to yield uncapped poly(A)(+)-RNA (Y....

Influenza virus NS1 protein stimulates translation of the M1 protein.

Enami, K, Sato, T A, Nakada, S, Enami, M

The influenza virus NS1 protein was shown to stimulate translation of the M1 protein. M-CAT RNA, which contains the chloramphenicol acetyltransferase (CAT) reporter gene and the terminal noncoding...

Induction of E1A-responsive negative factors for transcription of the fibronectin gene in adenovirus E1-transformed rat cells.

Nakamura, T, Nakajima, T, Tsunoda, S, Nakada, S, Oda, K, Tsurui, H, ...

The level of fibronectin (FN) gene expression is very high in resting rat 3Y1 cells but greatly decreased in adenovirus E1-transformed cells. To study the mechanism of this down-regulation, nuclear...

Influenza C virus RNA 7 codes for a nonstructural protein.

Nakada, S, Graves, P N, Desselberger, U, Creager, R S, Krystal, M, Palese, P

The complete nucleotide sequence of RNA segment 7 of influenza C/California/78 virus was determined by using cloned cDNA derived from viral RNA. The gene is 934 nucleotides long and possesses a long...

Expression of influenza virus NS2 nonstructural protein in bacteria and localization of NS2 in infected eucaryotic cells.

Greenspan, D, Krystal, M, Nakada, S, Arnheiter, H, Lyles, D S, Palese, P

The nonstructural NS2 protein of influenza A/PR/8/34 virus was efficiently expressed in bacteria, and monospecific antisera were prepared against the bacterially synthesized polypeptide. These...

Influenza C virus hemagglutinin: comparison with influenza A and B virus hemagglutinins.

Nakada, S, Creager, R S, Krystal, M, Aaronson, R P, Palese, P

The complete nucleotide sequence of the influenza C/California/78 virus RNA 4 was obtained by using cloned cDNA derived from the RNA segment. This gene is 2,071 nucleotides long and can code for a...

Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells.

Hara, E, Kato, T, Nakada, S, Sekiya, S, Oda, K

The human embryonal carcinoma cell line NEC14 can be induced to differentiate by the addition of 10(-2)M N,N'-hexamethylene-bis-acetamide (HMBA). A subtractive cDNA library specific to...

E1A-responsive elements for repression of rat fibronectin gene transcription.

Nakajima, T, Nakamura, T, Tsunoda, S, Nakada, S, Oda, K

The level of fibronectin (FN) gene transcription in resting rat 3Y1 cells is very high but decreases steeply after growth stimulation by serum or by the induction of E1A expression. To study the...

Regulation of influenza virus RNA polymerase activity by cellular and viral factors.

Shimizu, K, Handa, H, Nakada, S, Nagata, K

An in vitro RNA synthesis system mimicking replication of genomic influenza virus RNA was developed with nuclear extracts prepared from influenza virus-infected HeLa cells using exogenously added RNA...

The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis.

Nakagawa, Y, Kimura, N, Toyoda, T, Mizumoto, K, Ishihama, A, Oda, K, ...

An established cell line, clone 64, in which the expression of the RNA polymerase PB1 and PA subunit genes and the nucleoprotein (NP) gene but not the PB2 subunit gene of influenza virus can be...

The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.

Nakagawa, Y, Oda, K, Nakada, S

We indicated that the PB1 and PA subunits of RNA polymerase and nucleoprotein (NP) can support replication of the influenza virus genome as well as transcription to yield uncapped poly(A)(+)-RNA (Y....

Influenza virus NS1 protein stimulates translation of the M1 protein.

Enami, K, Sato, T A, Nakada, S, Enami, M

The influenza virus NS1 protein was shown to stimulate translation of the M1 protein. M-CAT RNA, which contains the chloramphenicol acetyltransferase (CAT) reporter gene and the terminal noncoding...

Induction of E1A-responsive negative factors for transcription of the fibronectin gene in adenovirus E1-transformed rat cells.

Nakamura, T, Nakajima, T, Tsunoda, S, Nakada, S, Oda, K, Tsurui, H, ...

The level of fibronectin (FN) gene expression is very high in resting rat 3Y1 cells but greatly decreased in adenovirus E1-transformed cells. To study the mechanism of this down-regulation, nuclear...

Influenza C virus RNA 7 codes for a nonstructural protein.

Nakada, S, Graves, P N, Desselberger, U, Creager, R S, Krystal, M, Palese, P

The complete nucleotide sequence of RNA segment 7 of influenza C/California/78 virus was determined by using cloned cDNA derived from viral RNA. The gene is 934 nucleotides long and possesses a long...

Expression of influenza virus NS2 nonstructural protein in bacteria and localization of NS2 in infected eucaryotic cells.

Greenspan, D, Krystal, M, Nakada, S, Arnheiter, H, Lyles, D S, Palese, P

The nonstructural NS2 protein of influenza A/PR/8/34 virus was efficiently expressed in bacteria, and monospecific antisera were prepared against the bacterially synthesized polypeptide. These...

Influenza C virus hemagglutinin: comparison with influenza A and B virus hemagglutinins.

Nakada, S, Creager, R S, Krystal, M, Aaronson, R P, Palese, P

The complete nucleotide sequence of the influenza C/California/78 virus RNA 4 was obtained by using cloned cDNA derived from the RNA segment. This gene is 2,071 nucleotides long and can code for a...

Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells.

Hara, E, Kato, T, Nakada, S, Sekiya, S, Oda, K

The human embryonal carcinoma cell line NEC14 can be induced to differentiate by the addition of 10(-2)M N,N'-hexamethylene-bis-acetamide (HMBA). A subtractive cDNA library specific to...

E1A-responsive elements for repression of rat fibronectin gene transcription.

Nakajima, T, Nakamura, T, Tsunoda, S, Nakada, S, Oda, K

The level of fibronectin (FN) gene transcription in resting rat 3Y1 cells is very high but decreases steeply after growth stimulation by serum or by the induction of E1A expression. To study the...

Regulation of influenza virus RNA polymerase activity by cellular and viral factors.

Shimizu, K, Handa, H, Nakada, S, Nagata, K

An in vitro RNA synthesis system mimicking replication of genomic influenza virus RNA was developed with nuclear extracts prepared from influenza virus-infected HeLa cells using exogenously added RNA...

IgG and IgE antibodies to Chironomidae in asthmatic patients.

Yamashita, N, Ito, K, Nakagawa, T, Haida, M, Okudaira, H, Nakada, S, ...

IgG antibodies to Chironomidae and its correlations to radioallergosorbent and skin reactions were examined with the aim of clarifying the relationship between asthma and Chironomidae. The level of...