Tyrosine–heme ligation in heme–peptide complex: design based on conserved motif of catalase (2007)
Rai, Jagdish, Raghothama, S, Sahal, D
On the basis of evolutionary conservation of sequence in catalases, we have designed a heme-binding peptide $(Ac-RLKSYTDTQISR^{12}-(GGGG)-CRIVHC^{22}-NH_2)$ for the 'redox activity modulation' of...
De novo Design of $\Delta F$ -containing Heme-binding Peptides (2007)
Rai, Jagdish, Raghothama, S, Sahal, D
The structural characterization of de novo designed metalloproteins together with determination of chemical reactivity can provide a detailed understanding of the relationship between protein...
Chakraborty, Kausik, Shivakumar, P, Raghothama, S, Varadarajan, Raghavan
gp120 is a subunit of the Env (viral envelope protein) of HIV-1. The protein consists of inner and outer domains linked by a bridging sheet. Several gp120 residues that bind the neutralizing antibody...
Tryptophan-containing peptide helices: interactions involving the indole side chain (2005)
Mahalakshmi, R, Sengupta, A, Raghothama, S, Shamala, N, Balaram, P
TTwo designed peptide sequences containing Trp residues at positions i and i + 5 ( Boc- Leu- Trp- Val- Ala- Aib- Leu- Trp- Val- OMe, 1) as well as i and i + 6 ( Boc- Leu- Trp- Val- Aib- Ala- Aib-...
Roy, Rituparna S, Gopi, Hosahudya N, Raghothama, S, Gilardi, Richard D, Karle, Isabella L, Balaram, Padmanabhan
The incporation of \beta -amino acid residues into the strand segments of designed \beta -hairpin leads to the formation of polar sheets, since in the case of \beta -peptide strands, all adjacent...
Roy, Rituparna S, Karle, Isabella L, Raghothama, S, Balaram, P
Conformational studies on the synthetic 11-aa peptide t-butoxycarbonyl (Boc)-Val-Ala-Phe-$\alpha$-aminoisobutyric acid (Aib)-(R)-$\beta^3$- homovaline ($\beta$Val)-(S)-$\beta^3$-homophenylalanine...
Solvent-induced Beta-hairpin to helix conformational transition in a designed peptide (2001)
Awasthi, Satish K, Shankaramma, SC, Raghothama, S, Balaram, P
An octapeptide containing a central Aib-Gly- segment capable of adopting -turn conformations compatible with both hairpin ( II or I ) and helical ( I) structures has been designed. The effect of...
Das, C, Nayak, V, Balaram, P, Raghothama, S
The characterization of a four-stranded $\beta$-sheet structure in a designed 26-residue peptide Beta-4 is described. The sequence of Beta-4...
A four stranded beta-sheet structure in a designed, synthetic polypeptide (1999)
Das, Chittaranjan, Raghothama, S, Balaram, P
A designed four stranded beta-sheet peptide has been constructed using three internal d-proline residues to nucleate beta-hairpin formation.
A Designed Three Stranded beta-Sheet Peptide as a Multiple beta-Hairpin Model (1998)
Das, Chittaranjan, Raghothama, S, Balaram, P
Beta-Hairpins are important elements in protein structure,1 implicated in the nucleation of beta-sheets in protein folding.2 Multiple hairpin structures, in which successive strands are connected by...
Raghothama, S, Chaddha, Manjula, Banumathi, S, Ravikumar, Krishnan, Velmurugan, D, Balaram, P
The crystal structure of the model tripeptide Boc-Aib-Gly-Leu-OMe (1) reveals two independent molecules in the asymmetric unit that adopt ‘‘enantiomeric’’ type I and type I’ beta-turn...
Peptide design. Helix–helix motifs in synthetic sequences (1997)
Banerjee, Arindam, Raghothama, S, Balaram, P
Two centrally positioned alpha-aminoisobutyryl (Aib) residues have been used to stabilize distinct heptapeptide helical segments in the 15-residue synthetic sequence...
Karle, Isabella L, Kaul, Ramesh, Rao, Balaji R, Raghothama, S, Balaram, P
The peptide Boc-Gly-Dpg-Gly-Gly-Dpg-Gly-NHMe (1) has been synthesized to examine the conformational preferences of Dpg residues in the context of a poor helix promoting sequence. Single crystals of 1...
A Designed beta-Hairpin Peptide (1995)
Awasthi, Satish K, Raghothama, S, Balaram, P
A synthetic octapeptide, Boc-Leu-Val-Val-D-Pro-Gly-Leu-Val-Val-OMe (1) has been designed as a model for a beta-hairpin conformation. Circular dichroism spectra in various organic solvents reveal a...
Raghothama, S, Ramakrishnan, C, Balasubramanian, D, Balaram, P
The conformation of cyclolinopeptide A [cyclo(Pro-Pro-Phe-Phe-Leu-Ile-Ile-Leu-Val)], a naturally occurring cyclic nonapeptide has been investigated in dimethylsulfoxide solution by 270 MHz 'H-nmr. A...
Krishnan, VV, Raghothama, S, Kumar, Anil
Heteronuclear shift correlation two-dimensional NMR spectroscopy (HETCOSY)(1-5) has become a powerful technique for resonance assignments of carbon and proton spectra, and for structural information...
Kishore, R, Raghothama, S, Balaram, P
A 20-membered cyclic peptide disulfide has been synthesized as a conformational model for disulfide loops of limited ring size. 1H-nmr studies at 270 MHz establish the presence of three...
Puranam, Kasturi L, Raghothama, S, Balaram, P
The solubilization of bilirubin IX\alpha (I) in aq. soln. by Na cholate micelles was examd. by 270-MHz 1H NMR spectroscopy. The incorporation of I into the micelles was accompanied by specific shifts...
Kishore, R, Raghothama, S, Balaram, P
The model cyclic pentapeptide disulfide Boc-Cys-Ala-Aib-Gly-Cys-NHMe 1 has been synthesized
Das, Manoj K, Raghothama, S, Balaram, P
The conformations of the 16-residue fungal peptide antiamoebin I were studied in DMSO soln. by 1- and 2-dimensional NMR techniques. A substantial no. of resonances in the 270-MHz 1H NMR spectrum were...
Solvent effects on the structure of benzo[b]selenophene oriented in liquid crystals (1984)
Surya Prakash, N., Raghothama, S., Khetrapal, C.L., Kunwar, A.C., Baiwir, M., Llabres, G.
Restricted Access.
Solvent effects on the structure of benzo[b]selenophene oriented in liquid crystals (1984)
Suryaprakash, N, Raghothama, S, Khetrapal, CL, Kunwar, AC, Baiwir, M, Llabres, G
Proton magnetic resonance spectra of benzo[b]selenophene including $^{77}Se$ satellites have been investigated in thermotropic liquid crystal solvents. Relative proton-proton and proton-selenium...
Roy, Rituparna S., Karle, Isabella L., Raghothama, S., Balaram, P.
Conformational studies on the synthetic 11-aa peptide t-butoxycarbonyl (Boc)-Val-Ala-Phe-α-aminoisobutyric acid (Aib)-(R)-β3-homovaline (βVal)-(S)-β3-homophenylalanine...
Chakraborty, Kausik, Shivakumar, P., Raghothama, S., Varadarajan, Raghavan
gp120 is a subunit of the Env (viral envelope protein) of HIV-1. The protein consists of inner and outer domains linked by a bridging sheet. Several gp120 residues that bind the neutralizing antibody...
Abou Hachem, M, Nordberg Karlsson, E, Bartonek-Roxâ, E, Raghothama, S, Simpson, P J, Gilbert, H J, ...
The two N-terminally repeated carbohydrate-binding modules (CBM4-1 and CBM4-2) encoded by xyn10A from Rhodothermus marinus were produced in Escherichia coli and purified by affinity chromatography....
Roy, Rituparna S., Karle, Isabella L., Raghothama, S., Balaram, P.
Conformational studies on the synthetic 11-aa peptide t-butoxycarbonyl (Boc)-Val-Ala-Phe-α-aminoisobutyric acid (Aib)-(R)-β3-homovaline (βVal)-(S)-β3-homophenylalanine...
Chakraborty, Kausik, Shivakumar, P., Raghothama, S., Varadarajan, Raghavan
gp120 is a subunit of the Env (viral envelope protein) of HIV-1. The protein consists of inner and outer domains linked by a bridging sheet. Several gp120 residues that bind the neutralizing antibody...
Abou Hachem, M, Nordberg Karlsson, E, Bartonek-Roxâ, E, Raghothama, S, Simpson, P J, Gilbert, H J, ...
The two N-terminally repeated carbohydrate-binding modules (CBM4-1 and CBM4-2) encoded by xyn10A from Rhodothermus marinus were produced in Escherichia coli and purified by affinity chromatography....