S. Spiegelman

ACTION OF CHEMOTHERAPEUTIC AGENTS AND SUBSTRATE ANALOGUES (1998)

SPIEGELMAN, S.

TO STUDY THE EFFECT OF SUBSTRATE ANALOGUES ON INDUCED SYNTHESIS OF ENZYMES (1998)

SPIEGELMAN, S.

The effect of tryptophan analogs on the induced synthesis of maltase and protein synthesis in yeast (1955)

Halvorson, Harlyn, Spiegelman, S., Hinman, R. L.

Peer Reviewed

THE INHIBITION OF ENZYME FORMATION BY AMINO ACID ANALOGUES1

Halvorson, Harlyn O., Spiegelman, S.

THE EFFECT OF FREE AMINO ACID POOL LEVELS ON THE INDUCED SYNTHESIS OF ENZYMES1

Halvorson, Harlyn O., Spiegelman, S.

NET UTILIZATION OF FREE AMINO ACIDS DURING THE INDUCED SYNTHESIS OF MALTOZYMASE IN YEAST1

Halvorson, Harlyn O., Spiegelman, S.

Specific template requirments of RNA replicases.

Haruna, I, Spiegelman, S

The synthesis of a self-propagating and infectious nucleic acid with a purified enzyme.

Spiegelman, S, Haruna, I, Holland, I B, Beaudreau, G, Mills, D

Recognition of size and sequence by an RNA replicase.

Haruna, I, Spiegelman, S

The in vitro translation of a monocistronic message.

Clark, J M, Chang, A Y, Spiegelman, S, Reichmann, M E

Selective interference with viral RNA formation in vitro by specific inhibition with synthetic polyribonucleotides.

Haruna, I, Spiegelman, S

THE IN VITRO SYNTHESIS OF A NONINFECTIOUS COMPLEX CONTAINING BIOLOGICALLY ACTIVE VIRAL RNA*

Mills, D. R., Pace, N. R., Spiegelman, S.

THE IDENTIFICATION OF THE RIBOSOMAL RNA CISTRON BY SEQUENCE COMPLEMENTARITY, I SPECIFICITY OF COMPLEX FORMATION*

Yankofsky, S. A., Spiegelman, S.

THE IDENTIFICATION OF THE RIBOSOMAL RNA CISTRON BY SEQUENCE COMPLEMENTARITY, II. SATURATION OF AND COMPETITIVE INTERACTION AT THE RNA CISTRON*

Yankofsky, S. A., Spiegelman, S.

LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER*

Ritossa, F. M., Spiegelman, S.

EVIDENCE FOR A NONRANDOM READING OF THE GENOME*

Kano-Sueoka, T., Spiegelman, S.

RESTRICTION OF IN VIVO GENETIC TRANSCRIPTION TO ONE OF THE COMPLEMENTARY STRANDS OF DNA*

Hayashi, M., Hayashi, M. N., Spiegelman, S.

SEQUENCE COMPLEMENTARITY OF T2-DNA AND T2-SPECIFIC RNA*

Hall, Benjamin D., Spiegelman, S.

AN RNA „REPLICASE” INDUCED BY AND SELECTIVE FOR A VIRAL RNA: ISOLATION AND PROPERTIES*

Haruna, I., Nozu, K., Ohtaka, Y., Spiegelman, S.

THE OCCURRENCE OF NATURAL DNA-RNA COMPLEXES IN E. COLI INFECTED WITH T2*

Spiegelman, S., Hall, Benjamin D., Storck, R.

THE SELECTIVE SYNTHESIS OF INFORMATIONAL RNA IN BACTERIA*

Hayashi, Masaki, Spiegelman, S.

FURTHER EXTRACELLULAR DARWINIAN EXPERIMENTS WITH REPLICATING RNA MOLECULES: DIVERSE VARIANTS ISOLATED UNDER DIFFERENT SELECTIVE CONDITIONS*

Levisohn, Reuben, Spiegelman, S.

Experiments are described which demonstrate that it is possible to isolate in vitro a variety of mutant RNA molecules which exhibit qualitatively distinguishable phenotypes. The results suggest that...

The mechanism of replication: a novel polarity reversal in the in vitro synthesis of Q-beta-RNA and its complement.

Bishop, D H, Pace, N R, Spiegelman, S

A search for an intermediate involving a complement during synchronous synthesis by a purified RNA replicase.

Haruna, I, Spiegelman, S

A rationale for an analysis of RNA replication.

Spiegelman, S, Haruna, I

The synthesis of infectious RNA with a replicase purified according to its size and density.

Pace, N R, Spiegelman, S

An analysis by gel electrophoresis of Q-beta-RNA complexes formed during the latent period of an in vitro synthesis.

Bishop, D H, Claybrook, J R, Pace, N R, Spiegelman, S

The dissociation of Q-beta-replicase and the relation of one of the components to a poly-C-dependent poly-G-polymerase.

Eikhom, T S, Spiegelman, S

Direct participation of a host protein in the replication of viral RNA in vitro.

Eikhom, T S, Stockley, D J, Spiegelman, S

The mechanism and direction of RNA synthesis templated by free minus strands of a "little" variant of Q-beta RNA.

Mills, D R, Bishop, H L, Spiegelman, S

The cloning of a self-replicating RNA molecule.

Levisohn, R, Spiegelman, S

Comparison of Two Serologically Distinct Ribonucleic Acid Bacteriophages II. Properties of the Nucleic Acids and Coat Proteins

Overby, L. R., Barlow, G. H., Doi, R. H., Jacob, Monique, Spiegelman, S.

Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. II. Properties of...

Qβ-Replicase and E. coli Transcriptase: Requirements for Substrate Selection as Revealed by a Study of Base Analogs*

Kapuler, A. M., Spiegelman, S.

Base selection by the Qβ-replicase and E. coli transcriptase has been studied using substrate analogs. Six analogs with normal hydrogen-bonding sites were polymerized by both enzymes. Three analogs...

RIBOSOME-BOUND β-GALACTOSIDASE

Cowie, D. B., Spiegelman, S., Roberts, R. B., Duerksen, J. D.

An estimation of genetic messages in the unfertilized echinoid egg.

Slater, D W, Spiegelman, S

The immediate precursor of viral RNA in the Q-beta-repicase reaction.

Pace, N R, Bishop, D H, Spiegelman, S

ISOLATION AND CHARACTERIZATION OF NUCLEAR BODIES FROM PROTOPLASTS OF BACILLUS MEGATERIUM1

Spiegelman, S., Aronson, A. I., Fitz-James, P. C.

CONSERVATION OF A VIRAL RNA GENOME DURING REPLICATION AND TRANSLATION*

Doi, R. H., Spiegelman, S.

DISTINCT CISTRONS FOR THE TWO RIBOSOMAL RNA COMPONENTS*

Yankofsky, S. A., Spiegelman, S.

SEPARATION OF THE RNA MESSAGE TRANSCRIBED IN RESPONSE TO A SPECIFIC INDUCER*

Hayashi, M., Spiegelman, S., Franklin, N. C., Luria, S. E.

DNA CIRCULARITY AND THE MECHANISM OF STRAND SELECTION IN THE GENERATION OF GENETIC MESSAGES*

Hayashi, M., Hayashi, M. N., Spiegelman, S.

THE ENZYMATIC SYNTHESIS OF A CIRCULAR DNA-RNA HYBRID*

Bassel, Alix, Hayashi, M., Spiegelman, S.

Comparison of Two Serologically Distinct Ribonucleic Acid Bacteriophages I. Properties of the Viral Particles

Overby, L. R., Barlow, G. H., Doi, R. H., Jacob, Monique, Spiegelman, S.

Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of...

THE CONVERSION OF NEGATIVES TO POSITIVES IN “SLOW” ADAPTING POPULATIONS OF YEAST, 12

Rotman, B., Spiegelman, S.

An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule.

Mills, D R, Peterson, R L, Spiegelman, S

The kinetics of product appearance and template involvement in the in vitro replication of viral RNA.

Pace, N R, Bishop, D H, Spiegelman, S

Specific cleavage of Qbeta-RNA and identification of the fragment carrying the 3'-OH terminus.

Bassel, B A, Spiegelman, S

Properties of retrovirus-like particles produced by a human breast carcinoma cell line: immunological relationship with mouse mammary tumor virus proteins.

Keydar, I, Ohno, T, Nayak, R, Sweet, R, Simoni, F, Weiss, F, ...

Clonal derivatives 8 and 11 of the T47D human breast carcinoma cell line release particles that have the biochemical characteristics of a retrovirus. Particles recovered from cultures of...

RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.

Myers, J C, Dobkin, C, Spiegelman, S

When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis...

Improving the anti-tumor activity of 5-fluorouracil by increasing its incorporation into RNA via metabolic modulation.

Spiegelman, S, Sawyer, R, Nayak, R, Ritzi, E, Stolfi, R, Martin, D

The experiments described here illustrate the use of metabolic modulation to improve the therapeutic effectiveness of 5-fluorouracil (5FUra) in two murine tumor systems (CD8F1 mannary carcinoma and...

Serological Analysis of the Deoxyribonucleic Acid Polymerase of Avian Oncornaviruses I. Preparation and Characterization of Monospecific Antiserum with Purified Deoxyribonucleic Acid Polymerase

Watson, K. F., Nowinski, R. C., Yaniv, A., Spiegelman, S.

Monospecific antiserum was prepared against purified deoxyribonucleic acid (DNA) polymerase from avian myeloblastosis virus (AMV). Immunodiffusion assay with purified DNA polymerase revealed that the...

Serological Analysis of the Deoxyribonucleic Acid Polymerase of Avian Oncornaviruses II. Comparison of Avian Deoxyribonucleic Acid Polymerases

Nowinski, Robert C., Watson, K. F., Yaniv, A., Spiegelman, S.

Monospecific antiserum prepared against the isolated deoxyribonucleic acid (DNA) polymerase of avian myeloblastosis virus (AMV) neutralized the endogenous ribonucleic acid-instructed DNA polymerase...

ON THE ROLE OF THE INDUCER IN THE SYNTHESIS OF MALTASE IN YEAST1

Spiegelman, S., Halvorson, Harlyn O.

ON THE ORIGIN OF THE CARBON IN THE INDUCED SYNTHESIS β-GALACTOSIDASE IN ESCHERICHIA COLI12

Rotman, B., Spiegelman, S.

The Relation of Sporulation and the Range of Variation of the Haplophase to Populational Adaptation1

Spiegelman, S., Lindegren, C. C.

Examination of the Qβ-Replicase Reaction by Sucrose Gradient and Gel Electrophoresis

Pace, N. R., Bishop, D. H. L., Spiegelman, S.

The products of an in vitro synthesis with the Qβ replicase purified from Escherichia coli infected by the ribonucleic acid (RNA) bacteriophage Qβ were examined by sucrose gradient centrifugation...

Deoxyribonucleic Acid Polymerase of Rous Sarcoma Virus: Reaction Conditions and Analysis of the Reaction Product Nucleic Acids

Bishop, D. H. L., Ruprecht, Ruth, Simpson, R. W., Spiegelman, S.

Reaction conditions for Rous sarcoma virus ribonucleic acid (RNA)-instructed deoxyribonucleic acid (DNA) polymerase activity are described whereby the viral RNA is relatively protected from...

Human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus.

Ohno, T, Mesa-Tejada, R, Keydar, I, Ramanarayanan, M, Bausch, J, Spiegelman, S

We have shown [Mesa-Tejada, R., Keydar, I., Ramanarayanan, M., Ohno, T., Fenoglio, C. & Spiegelman, S. (1978) Proc. Natl. Acad. Sci. USA 75, 1529--1533] that an antigen immunologically related to...

Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase

Gulati, S. C., Kacian, D. L., Spiegelman, S.

Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The...

The Presence of Unique DNA Sequences after Viral Induction of Leukemia in Mice

Sweet, R. W., Goodman, N. C., Ruprecht, R. M., Redfield, R. R., Spiegelman, S.

From previous studies, lymphocyte DNA from human leukemias and DNA from involved tissues of patients with Hodgkin's disease or Burkitt's lymphoma contain sequences that are absent from their normal...

Evidence of Particle-Associated RNA-Directed DNA Polymerase and High Molecular Weight RNA in Human Gastrointestinal and Lung Malignancies

Cuatico, W., Spiegelman, S.

Previous communications have demonstrated that neoplastic cells of human breast cancers, leukemias, lymphomas, sarcomas, and brain tumors contain particles with similar diagnostic attributes as those...

DNA Polymerase Activities and Nucleic Acid Components of Virions Isolated from a Spontaneous Mammary Carcinoma from a Rhesus Monkey

Schlom, J., Spiegelman, S.

Virions isolated from a spontaneous mammary carcinoma of a rhesus monkey and propagated in human cells possess an RNA-instructed DNA polymerase. They also exhibit DNA polymerase activities that...

Distinguishing Reverse Transcriptase of an RNA Tumor Virus from Other Known DNA Polymerases

Goodman, N. C., Spiegelman, S.

Assays are described that permit one to distinguish the reverse transcriptase of RNA tumor viruses from known normal cellular DNA-instructed DNA polymerases. Template responses of purified reverse...

Synthesis of DNA Complements of Natural RNAs: A General Approach

Spiegelman, S., Watson, K. F., Kacian, D. L.

The availability of a purified RNA-instructed DNA polymerase (reverse transcriptase) from avain myeloblastosis virus provided the opportunity to explore whether this enzyme could be used as a general...

A Replicating RNA Molecule Suitable for a Detailed Analysis of Extracellular Evolution and Replication

Kacian, D. L., Mills, D. R., Kramer, F. R., Spiegelman, S.

The aim of the present study is to make available a replicating molecule of known sequence. Accordingly, we sought a molecule that has the following properties: (a) replicates in vitro in a manner...

Particles Containing RNA-Instructed DNA Polymerase and Virus-Related RNA in Human Breast Cancers

Axel, R., Gulati, S. C., Spiegelman, S.

Human breast cancers contain an RNA related to that of mouse mammary tumor virus. In 79% of the breast malignancies examined, this type of RNA is a 70S-component encapsulated with RNA-instructed DNA...

Nuclear DNA Sequences Present in Human Leukemic Cells and Absent in Normal Leukocytes

Baxt, W. G., Spiegelman, S.

The central purpose of the present study was to test the proposition that the nuclear DNA of every human cell contains whatever information is necessary and sufficient for transformation to...

DNA Polymerase Activities in Virions of Visna Virus, a Causative Agent of a “Slow” Neurological Disease

Schlom, J., Harter, D. H., Burny, A., Spiegelman, S.

The presence is reported of an RNA-instructed DNA polymerase in visna virus, the causative agent of a “slow” neurological disease in sheep. The product synthesized by the RNA-directed reaction...

Purification and characterization of the DNA polymerase of human breast cancer particles.

Ohno, T, Sweet, R W, Dejak, D, Spiegelman, S

Previous studies have identified human breast tumor particles possessing many of the features characteristic of RNA tumor viruses. In addition to the expected size (600 S) and density (1.16 g/ml)...

Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.

Myers, J C, Spiegelman, S

Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) catalyzed reaction. In the presence of...

Detection of viral proteins in mouse mammary tumors by immunoperoxidase staining of paraffin sections.

Keydar, I, Mesa-Tejada, R, Ramanarayanan, M, Ohno, T, Fenoglio, C, Hu, R, ...

An indirect immunoperoxidase method is described, which can readily detect viral antigens in paraffin sections of primary, transplanted, and metastatic mammary tumors of mice. In addition to having...

Detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus

Mesa-Tejada, R., Keydar, I., Ramanarayanan, M., Ohno, T., Fenoglio, C., Spiegelman, S.

An antigen immunologically related to a group-specific antigen (gp52, a 52,000-dalton glycoprotein) of the mouse mammary tumor virus has been identified in paraffin sections of human breast cancers...

RNA in Human Leukemic Cells Related to the RNA of a Mouse Leukemia Virus

Hehlmann, R., Kufe, D., Spiegelman, S.

Molecular hybridization with radioactively labeled DNA complementary to the RNA of the Rauscher leukemia virus was used to probe for homologous RNA in the polysome fraction of human leukemic cells....

Evidence for Translation of Viral-Specific RNA in Cells of a Mouse Mammary Carcinoma

Axel, R., Schlom, J., Spiegelman, S.

A procedure is described permitting the detection of viral-specific RNA in a mouse mammary tumor. The method involves molecular hybridization with radioactively labeled DNA complementary to the RNA...

Viral-Related RNA in Hodgkins' Disease and Other Human Lymphomas

Hehlmann, R., Kufe, D., Spiegelman, S.

Molecular hybridization with radioactively labeled DNA complementary to the RNA of the Rauscher leukemia virus was used to probe for homologous RNA in human lymphomas. 22 of 32 specimens contained...

Detection of RNA-Instructed DNA Polymerase and High Molecular Weight RNA in Malignant Tissue

Gulati, S. C., Axel, R., Spiegelman, S.

An experimental procedure is detailed that permits the detection of 70S RNA-directed DNA synthesis in mouse mammary carcinomas. The DNA synthesized is complementary to the RNA of the mouse mammary...

Leukemia-Specific DNA Sequences in Leukocytes of the Leukemic Member of Identical Twins

Baxt, W., Yates, J. W., Wallace, H. J., Holland, J. F., Spiegelman, S.

The discovery in human leukemic cells of particulate elements encapsulating 70S RNA and RNA-directed DNA polymerase made possible the synthesis of a [3H]DNA probe that could detect leukemia-specific...

Particles with RNA of High Molecular Weight and RNA-Directed DNA Polymerase in Human Brain Tumors

Cuatico, W., Spiegelman, S.

We have previously shown that neoplastic cells of human breast cancers, leukemias, lymphomas, and sarcomas contain particles similar to the viruses that have been established as etiologic agents of...

Biological and Biochemical Evidence for an Interaction Between Marek's Disease Herpesvirus and Avian Leukosis Virus In Vivo

Peters, W. P., Kufe, D., Schlom, J., Frankel, J. W., Prickett, C. O., Groupé, V., ...

The DNA-containing Epstein-Barr herpesvirus has been implicated in the etiology of Burkitt's lymphoma, a malignant tumor of children in Africa. Recently, however, particles possessing four...

Unique Nuclear DNA Sequences in the Involved Tissues of Hodgkin's and Burkitt's Lymphomas

Kufe, D. W., Peters, W. P., Spiegelman, S.

Previous studies have demonstrated that Hodgkin's, Burkitt's, and other human lymphomas contain particulate elements encapsulating 70S RNA and RNA-dependent DNA polymerase. [3H]DNA probes...

Antigenic relatedness of the DNA polymerase of human breast cancer particles to the enzyme of the Mason-Pfizer monkey virus.

Ohno, T, Spiegelman, S

We have previously reported [(Ohno, T., Sweet, R.W., Hu, R., DeJak, D. & Spiegelman, S. (1977) Proc. Natl. Acad. Sci. USA 74, 764-768)] on the purification and characterization of the DNA polymerase...

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.

Myers, J C, Spiegelman, S, Kacian, D L

The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight...

Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model.

Ritzi, E, Martin, D S, Stolfi, R L, Spiegelman, S

We used the mouse mammary tumor and its associated virus (murine mammary tumor virus) to examine the possibility of using plasma levels of a viral protein (gp52, the glycoprotein of 52,000 molecular...

Oncornavirus-like particles in human skin cancers.

Balda, B R, Hehlmann, R, Cho, J R, Spiegelman, S

A high-molecular-weight RNA encapsulated with an RNA-instructed DNA polymerase in particles possessing the density characteristic of the RNA tumor viruses has been detected in 13 out of 14 human...

Purification of RNA-instructed DNA polymerase from human leukemic spleens.

Witkin, S S, Ohno, T, Spiegelman, S

Particles possessing a density of 1.16 g/ml and encapsulating a 70S RNA and a RNA-instructed DNA polymerase (reverse transcriptase) have been prepared from the spleen of a patient with chronic...

RNA Related to That of a Murine Leukemia Virus in Burkitt's Tumors and Nasopharyngeal Carcinomas

Kufe, D., Hehlmann, R., Spiegelman, S.

RNA homologous to that of the Rauscher leukemia virus has been detected in Burkitt's lymphomas and nasopharyngeal carcinomas. Earlier excellent experimental evidence has linked these two human tumors...

Burkitt's Tumors Contain Particles Encapsulating RNA-Instructed DNA Polymerase and High Molecular Weight Virus-Related RNA

Kufe, D., Magrath, I. T., Ziegler, J. L., Spiegelman, S.

Burkitt's lymphomas, linked by previous studies with the DNA-containing Epstein-Barr virus, contain an RNA related in sequence to that of Rauscher leukemia virus. The present study establishes that...

Determination of Natural Host Taxonomy of RNA Tumor Viruses by Molecular Hybridization: Application to RD-114, a Candidate Human Virus*

Ruprecht, Ruth M., Goodman, N. C., Spiegelman, S.

The lysogenic bacteriophages and the RNA tumor viruses have in common the ability to add their genetic information to the genome of their host cells. The biological similarity extends further; data...

Decreased Globin Messenger RNA in Thalassemia Detected by Molecular Hybridization

Kacian, D. L., Gambino, R., Dow, L. W., Grossbard, E., Natta, C., Ramirez, F., ...

In previous studies of patients with β thalassemia, mRNA extracted from reticulocytes in peripheral blood when added to cell-free systems reproduces the deficient β-chain synthesis characteristic...

Further Evidence for Oncornaviruses in Human Milk: The Production of Cores

Feldman, Stuart P., Schlom, J., Spiegelman, S.

Cores, or nucleoids, have been isolated from particles in human milk. The cores have a density of 1.26-1.27 g/ml and contain a 60-70S RNA in association with an RNA-instructed DNA polymerase. The...

Sequence Homology Between the RNA of Mason-Pfizer Monkey Virus and the RNA of Human Malignant Breast Tumors

Colcher, David, Spiegelman, S., Schlom, Jeffrey

Radioactive DNA ([3H]cDNA) complementary to the RNA of Mason-Pfizer monkey virus was used in molecular hybridization experiments to demonstrate sequence homology between its viral RNA and RNA of...

ENZYME FORMATION IN PROTOPLASTS OF BACILLUS MEGATERIUM*

Landman, O. E., Spiegelman, S.

On the Cytoplasmic Nature of “Long-Term Adaptation” in Yeast*

Spiegelman, S., Sussman, Racquel Rotman, Pinska, E.

A Single-Cell Analysis of the Transmission of Enzyme-Forming Capacity in Yeast*

Spiegelman, S., DeLorenzo, W. F., Campbell, A. M.

Substrate Stabilization of Enzyme-Forming Capacity During the Segregation of a Heterozygote*

Spiegelman, S., DeLorenzo, W. F.

Mechanism of Enzymatic Adaptation in Genetically Controlled Yeast Populations

Spiegelman, S., Lindegren, Carl C., Hedgecock, L.

Mendelian Inheritance of Adaptive Enzymes*

Lindegren, Carl C., Spiegelman, S., Lindegren, Gertrude

Maintenance and Increase of a Genetic Character by a Substrate-Cytoplasmic Interaction in the Absence of the Specific Gene*

Spiegelman, S., Lindegren, Carl C., Lindegren, Gertrude

Size Distribution of "Informational" RNA

Sagik, B. P., Green, M. H., Hayashi, M., Spiegelman, S.

Previous investigations suggested that the size of “informational” or “messenger” RNA was confined to sedimentation rates lying between 8 and 14S. These involved procedures permitting...

Chromatographic Separation of Annealed and Enzymatically Synthesized RNA-DNA Hybrids

Hayashi, Marie N., Hayashi, M., Spiegelman, S.

A procedure is described for the chromatographic detection and isolation of DNA-RNA hybrids on columns of methylated albumin coated on kieselguhr (MAK). Its use is illustrated with both annealed and...

A Chemical and Physical Characterization of Echinoid RNA during Early Embryogenesis

Slater, D. W., Spiegelman, S.

The properties of the major RNA components prepared from Arbacia punctulata have been characterized by sedimentation in sucrose gradients and analysis of base composition. Comparison of the...

On the Redundancy of DNA Complementary to Amino Acid Transfer RNA and Its Absence from the Nucleolar Organizer Region of DROSOPHILA MELANOGASTER

Ritossa, F. M., Atwood, K. C., Spiegelman, S.

A Molecular Explanation of the Bobbed Mutants of Drosophila as Partial Deficiencies of "Ribosomal" DNA

Ritossa, F. M., Atwood, K. C., Spiegelman, S.

THE INHIBITION OF ENZYME FORMATION BY AMINO ACID ANALOGUES1

Halvorson, Harlyn O., Spiegelman, S.

THE EFFECT OF FREE AMINO ACID POOL LEVELS ON THE INDUCED SYNTHESIS OF ENZYMES1

Halvorson, Harlyn O., Spiegelman, S.

NET UTILIZATION OF FREE AMINO ACIDS DURING THE INDUCED SYNTHESIS OF MALTOZYMASE IN YEAST1

Halvorson, Harlyn O., Spiegelman, S.

Specific template requirments of RNA replicases.

Haruna, I, Spiegelman, S

The synthesis of a self-propagating and infectious nucleic acid with a purified enzyme.

Spiegelman, S, Haruna, I, Holland, I B, Beaudreau, G, Mills, D

Recognition of size and sequence by an RNA replicase.

Haruna, I, Spiegelman, S

The in vitro translation of a monocistronic message.

Clark, J M, Chang, A Y, Spiegelman, S, Reichmann, M E

Selective interference with viral RNA formation in vitro by specific inhibition with synthetic polyribonucleotides.

Haruna, I, Spiegelman, S

THE IN VITRO SYNTHESIS OF A NONINFECTIOUS COMPLEX CONTAINING BIOLOGICALLY ACTIVE VIRAL RNA*

Mills, D. R., Pace, N. R., Spiegelman, S.

THE IDENTIFICATION OF THE RIBOSOMAL RNA CISTRON BY SEQUENCE COMPLEMENTARITY, I SPECIFICITY OF COMPLEX FORMATION*

Yankofsky, S. A., Spiegelman, S.

THE IDENTIFICATION OF THE RIBOSOMAL RNA CISTRON BY SEQUENCE COMPLEMENTARITY, II. SATURATION OF AND COMPETITIVE INTERACTION AT THE RNA CISTRON*

Yankofsky, S. A., Spiegelman, S.

LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER*

Ritossa, F. M., Spiegelman, S.

EVIDENCE FOR A NONRANDOM READING OF THE GENOME*

Kano-Sueoka, T., Spiegelman, S.

RESTRICTION OF IN VIVO GENETIC TRANSCRIPTION TO ONE OF THE COMPLEMENTARY STRANDS OF DNA*

Hayashi, M., Hayashi, M. N., Spiegelman, S.

SEQUENCE COMPLEMENTARITY OF T2-DNA AND T2-SPECIFIC RNA*

Hall, Benjamin D., Spiegelman, S.

AN RNA „REPLICASE” INDUCED BY AND SELECTIVE FOR A VIRAL RNA: ISOLATION AND PROPERTIES*

Haruna, I., Nozu, K., Ohtaka, Y., Spiegelman, S.

THE OCCURRENCE OF NATURAL DNA-RNA COMPLEXES IN E. COLI INFECTED WITH T2*

Spiegelman, S., Hall, Benjamin D., Storck, R.

THE SELECTIVE SYNTHESIS OF INFORMATIONAL RNA IN BACTERIA*

Hayashi, Masaki, Spiegelman, S.

FURTHER EXTRACELLULAR DARWINIAN EXPERIMENTS WITH REPLICATING RNA MOLECULES: DIVERSE VARIANTS ISOLATED UNDER DIFFERENT SELECTIVE CONDITIONS*

Levisohn, Reuben, Spiegelman, S.

Experiments are described which demonstrate that it is possible to isolate in vitro a variety of mutant RNA molecules which exhibit qualitatively distinguishable phenotypes. The results suggest that...

The mechanism of replication: a novel polarity reversal in the in vitro synthesis of Q-beta-RNA and its complement.

Bishop, D H, Pace, N R, Spiegelman, S

A search for an intermediate involving a complement during synchronous synthesis by a purified RNA replicase.

Haruna, I, Spiegelman, S

A rationale for an analysis of RNA replication.

Spiegelman, S, Haruna, I

The synthesis of infectious RNA with a replicase purified according to its size and density.

Pace, N R, Spiegelman, S

An analysis by gel electrophoresis of Q-beta-RNA complexes formed during the latent period of an in vitro synthesis.

Bishop, D H, Claybrook, J R, Pace, N R, Spiegelman, S

The dissociation of Q-beta-replicase and the relation of one of the components to a poly-C-dependent poly-G-polymerase.

Eikhom, T S, Spiegelman, S

Direct participation of a host protein in the replication of viral RNA in vitro.

Eikhom, T S, Stockley, D J, Spiegelman, S

The mechanism and direction of RNA synthesis templated by free minus strands of a "little" variant of Q-beta RNA.

Mills, D R, Bishop, H L, Spiegelman, S

The cloning of a self-replicating RNA molecule.

Levisohn, R, Spiegelman, S

Comparison of Two Serologically Distinct Ribonucleic Acid Bacteriophages II. Properties of the Nucleic Acids and Coat Proteins

Overby, L. R., Barlow, G. H., Doi, R. H., Jacob, Monique, Spiegelman, S.

Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. II. Properties of...

Qβ-Replicase and E. coli Transcriptase: Requirements for Substrate Selection as Revealed by a Study of Base Analogs*

Kapuler, A. M., Spiegelman, S.

Base selection by the Qβ-replicase and E. coli transcriptase has been studied using substrate analogs. Six analogs with normal hydrogen-bonding sites were polymerized by both enzymes. Three analogs...

RIBOSOME-BOUND β-GALACTOSIDASE

Cowie, D. B., Spiegelman, S., Roberts, R. B., Duerksen, J. D.

An estimation of genetic messages in the unfertilized echinoid egg.

Slater, D W, Spiegelman, S

The immediate precursor of viral RNA in the Q-beta-repicase reaction.

Pace, N R, Bishop, D H, Spiegelman, S

ISOLATION AND CHARACTERIZATION OF NUCLEAR BODIES FROM PROTOPLASTS OF BACILLUS MEGATERIUM1

Spiegelman, S., Aronson, A. I., Fitz-James, P. C.

CONSERVATION OF A VIRAL RNA GENOME DURING REPLICATION AND TRANSLATION*

Doi, R. H., Spiegelman, S.

DISTINCT CISTRONS FOR THE TWO RIBOSOMAL RNA COMPONENTS*

Yankofsky, S. A., Spiegelman, S.

SEPARATION OF THE RNA MESSAGE TRANSCRIBED IN RESPONSE TO A SPECIFIC INDUCER*

Hayashi, M., Spiegelman, S., Franklin, N. C., Luria, S. E.

DNA CIRCULARITY AND THE MECHANISM OF STRAND SELECTION IN THE GENERATION OF GENETIC MESSAGES*

Hayashi, M., Hayashi, M. N., Spiegelman, S.

THE ENZYMATIC SYNTHESIS OF A CIRCULAR DNA-RNA HYBRID*

Bassel, Alix, Hayashi, M., Spiegelman, S.

Comparison of Two Serologically Distinct Ribonucleic Acid Bacteriophages I. Properties of the Viral Particles

Overby, L. R., Barlow, G. H., Doi, R. H., Jacob, Monique, Spiegelman, S.

Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of...

THE CONVERSION OF NEGATIVES TO POSITIVES IN “SLOW” ADAPTING POPULATIONS OF YEAST, 12

Rotman, B., Spiegelman, S.

An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule.

Mills, D R, Peterson, R L, Spiegelman, S

The kinetics of product appearance and template involvement in the in vitro replication of viral RNA.

Pace, N R, Bishop, D H, Spiegelman, S

Specific cleavage of Qbeta-RNA and identification of the fragment carrying the 3'-OH terminus.

Bassel, B A, Spiegelman, S

Properties of retrovirus-like particles produced by a human breast carcinoma cell line: immunological relationship with mouse mammary tumor virus proteins.

Keydar, I, Ohno, T, Nayak, R, Sweet, R, Simoni, F, Weiss, F, ...

Clonal derivatives 8 and 11 of the T47D human breast carcinoma cell line release particles that have the biochemical characteristics of a retrovirus. Particles recovered from cultures of...

RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.

Myers, J C, Dobkin, C, Spiegelman, S

When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis...

Improving the anti-tumor activity of 5-fluorouracil by increasing its incorporation into RNA via metabolic modulation.

Spiegelman, S, Sawyer, R, Nayak, R, Ritzi, E, Stolfi, R, Martin, D

The experiments described here illustrate the use of metabolic modulation to improve the therapeutic effectiveness of 5-fluorouracil (5FUra) in two murine tumor systems (CD8F1 mannary carcinoma and...

Serological Analysis of the Deoxyribonucleic Acid Polymerase of Avian Oncornaviruses I. Preparation and Characterization of Monospecific Antiserum with Purified Deoxyribonucleic Acid Polymerase

Watson, K. F., Nowinski, R. C., Yaniv, A., Spiegelman, S.

Monospecific antiserum was prepared against purified deoxyribonucleic acid (DNA) polymerase from avian myeloblastosis virus (AMV). Immunodiffusion assay with purified DNA polymerase revealed that the...

Serological Analysis of the Deoxyribonucleic Acid Polymerase of Avian Oncornaviruses II. Comparison of Avian Deoxyribonucleic Acid Polymerases

Nowinski, Robert C., Watson, K. F., Yaniv, A., Spiegelman, S.

Monospecific antiserum prepared against the isolated deoxyribonucleic acid (DNA) polymerase of avian myeloblastosis virus (AMV) neutralized the endogenous ribonucleic acid-instructed DNA polymerase...

ON THE ROLE OF THE INDUCER IN THE SYNTHESIS OF MALTASE IN YEAST1

Spiegelman, S., Halvorson, Harlyn O.

ON THE ORIGIN OF THE CARBON IN THE INDUCED SYNTHESIS β-GALACTOSIDASE IN ESCHERICHIA COLI12

Rotman, B., Spiegelman, S.

The Relation of Sporulation and the Range of Variation of the Haplophase to Populational Adaptation1

Spiegelman, S., Lindegren, C. C.

Examination of the Qβ-Replicase Reaction by Sucrose Gradient and Gel Electrophoresis

Pace, N. R., Bishop, D. H. L., Spiegelman, S.

The products of an in vitro synthesis with the Qβ replicase purified from Escherichia coli infected by the ribonucleic acid (RNA) bacteriophage Qβ were examined by sucrose gradient centrifugation...

Deoxyribonucleic Acid Polymerase of Rous Sarcoma Virus: Reaction Conditions and Analysis of the Reaction Product Nucleic Acids

Bishop, D. H. L., Ruprecht, Ruth, Simpson, R. W., Spiegelman, S.

Reaction conditions for Rous sarcoma virus ribonucleic acid (RNA)-instructed deoxyribonucleic acid (DNA) polymerase activity are described whereby the viral RNA is relatively protected from...

Human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus.

Ohno, T, Mesa-Tejada, R, Keydar, I, Ramanarayanan, M, Bausch, J, Spiegelman, S

We have shown [Mesa-Tejada, R., Keydar, I., Ramanarayanan, M., Ohno, T., Fenoglio, C. & Spiegelman, S. (1978) Proc. Natl. Acad. Sci. USA 75, 1529--1533] that an antigen immunologically related to...

Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase

Gulati, S. C., Kacian, D. L., Spiegelman, S.

Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The...

The Presence of Unique DNA Sequences after Viral Induction of Leukemia in Mice

Sweet, R. W., Goodman, N. C., Ruprecht, R. M., Redfield, R. R., Spiegelman, S.

From previous studies, lymphocyte DNA from human leukemias and DNA from involved tissues of patients with Hodgkin's disease or Burkitt's lymphoma contain sequences that are absent from their normal...

Evidence of Particle-Associated RNA-Directed DNA Polymerase and High Molecular Weight RNA in Human Gastrointestinal and Lung Malignancies

Cuatico, W., Spiegelman, S.

Previous communications have demonstrated that neoplastic cells of human breast cancers, leukemias, lymphomas, sarcomas, and brain tumors contain particles with similar diagnostic attributes as those...

DNA Polymerase Activities and Nucleic Acid Components of Virions Isolated from a Spontaneous Mammary Carcinoma from a Rhesus Monkey

Schlom, J., Spiegelman, S.

Virions isolated from a spontaneous mammary carcinoma of a rhesus monkey and propagated in human cells possess an RNA-instructed DNA polymerase. They also exhibit DNA polymerase activities that...

Distinguishing Reverse Transcriptase of an RNA Tumor Virus from Other Known DNA Polymerases

Goodman, N. C., Spiegelman, S.

Assays are described that permit one to distinguish the reverse transcriptase of RNA tumor viruses from known normal cellular DNA-instructed DNA polymerases. Template responses of purified reverse...

Synthesis of DNA Complements of Natural RNAs: A General Approach

Spiegelman, S., Watson, K. F., Kacian, D. L.

The availability of a purified RNA-instructed DNA polymerase (reverse transcriptase) from avain myeloblastosis virus provided the opportunity to explore whether this enzyme could be used as a general...

A Replicating RNA Molecule Suitable for a Detailed Analysis of Extracellular Evolution and Replication

Kacian, D. L., Mills, D. R., Kramer, F. R., Spiegelman, S.

The aim of the present study is to make available a replicating molecule of known sequence. Accordingly, we sought a molecule that has the following properties: (a) replicates in vitro in a manner...

Particles Containing RNA-Instructed DNA Polymerase and Virus-Related RNA in Human Breast Cancers

Axel, R., Gulati, S. C., Spiegelman, S.

Human breast cancers contain an RNA related to that of mouse mammary tumor virus. In 79% of the breast malignancies examined, this type of RNA is a 70S-component encapsulated with RNA-instructed DNA...

Nuclear DNA Sequences Present in Human Leukemic Cells and Absent in Normal Leukocytes

Baxt, W. G., Spiegelman, S.

The central purpose of the present study was to test the proposition that the nuclear DNA of every human cell contains whatever information is necessary and sufficient for transformation to...

DNA Polymerase Activities in Virions of Visna Virus, a Causative Agent of a “Slow” Neurological Disease

Schlom, J., Harter, D. H., Burny, A., Spiegelman, S.

The presence is reported of an RNA-instructed DNA polymerase in visna virus, the causative agent of a “slow” neurological disease in sheep. The product synthesized by the RNA-directed reaction...

Purification and characterization of the DNA polymerase of human breast cancer particles.

Ohno, T, Sweet, R W, Dejak, D, Spiegelman, S

Previous studies have identified human breast tumor particles possessing many of the features characteristic of RNA tumor viruses. In addition to the expected size (600 S) and density (1.16 g/ml)...

Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.

Myers, J C, Spiegelman, S

Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) catalyzed reaction. In the presence of...

Detection of viral proteins in mouse mammary tumors by immunoperoxidase staining of paraffin sections.

Keydar, I, Mesa-Tejada, R, Ramanarayanan, M, Ohno, T, Fenoglio, C, Hu, R, ...

An indirect immunoperoxidase method is described, which can readily detect viral antigens in paraffin sections of primary, transplanted, and metastatic mammary tumors of mice. In addition to having...

Detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus

Mesa-Tejada, R., Keydar, I., Ramanarayanan, M., Ohno, T., Fenoglio, C., Spiegelman, S.

An antigen immunologically related to a group-specific antigen (gp52, a 52,000-dalton glycoprotein) of the mouse mammary tumor virus has been identified in paraffin sections of human breast cancers...

RNA in Human Leukemic Cells Related to the RNA of a Mouse Leukemia Virus

Hehlmann, R., Kufe, D., Spiegelman, S.

Molecular hybridization with radioactively labeled DNA complementary to the RNA of the Rauscher leukemia virus was used to probe for homologous RNA in the polysome fraction of human leukemic cells....

Evidence for Translation of Viral-Specific RNA in Cells of a Mouse Mammary Carcinoma

Axel, R., Schlom, J., Spiegelman, S.

A procedure is described permitting the detection of viral-specific RNA in a mouse mammary tumor. The method involves molecular hybridization with radioactively labeled DNA complementary to the RNA...

Viral-Related RNA in Hodgkins' Disease and Other Human Lymphomas

Hehlmann, R., Kufe, D., Spiegelman, S.

Molecular hybridization with radioactively labeled DNA complementary to the RNA of the Rauscher leukemia virus was used to probe for homologous RNA in human lymphomas. 22 of 32 specimens contained...

Detection of RNA-Instructed DNA Polymerase and High Molecular Weight RNA in Malignant Tissue

Gulati, S. C., Axel, R., Spiegelman, S.

An experimental procedure is detailed that permits the detection of 70S RNA-directed DNA synthesis in mouse mammary carcinomas. The DNA synthesized is complementary to the RNA of the mouse mammary...

Leukemia-Specific DNA Sequences in Leukocytes of the Leukemic Member of Identical Twins

Baxt, W., Yates, J. W., Wallace, H. J., Holland, J. F., Spiegelman, S.

The discovery in human leukemic cells of particulate elements encapsulating 70S RNA and RNA-directed DNA polymerase made possible the synthesis of a [3H]DNA probe that could detect leukemia-specific...

Particles with RNA of High Molecular Weight and RNA-Directed DNA Polymerase in Human Brain Tumors

Cuatico, W., Spiegelman, S.

We have previously shown that neoplastic cells of human breast cancers, leukemias, lymphomas, and sarcomas contain particles similar to the viruses that have been established as etiologic agents of...

Biological and Biochemical Evidence for an Interaction Between Marek's Disease Herpesvirus and Avian Leukosis Virus In Vivo

Peters, W. P., Kufe, D., Schlom, J., Frankel, J. W., Prickett, C. O., Groupé, V., ...

The DNA-containing Epstein-Barr herpesvirus has been implicated in the etiology of Burkitt's lymphoma, a malignant tumor of children in Africa. Recently, however, particles possessing four...

Unique Nuclear DNA Sequences in the Involved Tissues of Hodgkin's and Burkitt's Lymphomas

Kufe, D. W., Peters, W. P., Spiegelman, S.

Previous studies have demonstrated that Hodgkin's, Burkitt's, and other human lymphomas contain particulate elements encapsulating 70S RNA and RNA-dependent DNA polymerase. [3H]DNA probes...

Antigenic relatedness of the DNA polymerase of human breast cancer particles to the enzyme of the Mason-Pfizer monkey virus.

Ohno, T, Spiegelman, S

We have previously reported [(Ohno, T., Sweet, R.W., Hu, R., DeJak, D. & Spiegelman, S. (1977) Proc. Natl. Acad. Sci. USA 74, 764-768)] on the purification and characterization of the DNA polymerase...

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.

Myers, J C, Spiegelman, S, Kacian, D L

The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight...

Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model.

Ritzi, E, Martin, D S, Stolfi, R L, Spiegelman, S

We used the mouse mammary tumor and its associated virus (murine mammary tumor virus) to examine the possibility of using plasma levels of a viral protein (gp52, the glycoprotein of 52,000 molecular...

Oncornavirus-like particles in human skin cancers.

Balda, B R, Hehlmann, R, Cho, J R, Spiegelman, S

A high-molecular-weight RNA encapsulated with an RNA-instructed DNA polymerase in particles possessing the density characteristic of the RNA tumor viruses has been detected in 13 out of 14 human...

Purification of RNA-instructed DNA polymerase from human leukemic spleens.

Witkin, S S, Ohno, T, Spiegelman, S

Particles possessing a density of 1.16 g/ml and encapsulating a 70S RNA and a RNA-instructed DNA polymerase (reverse transcriptase) have been prepared from the spleen of a patient with chronic...

RNA Related to That of a Murine Leukemia Virus in Burkitt's Tumors and Nasopharyngeal Carcinomas

Kufe, D., Hehlmann, R., Spiegelman, S.

RNA homologous to that of the Rauscher leukemia virus has been detected in Burkitt's lymphomas and nasopharyngeal carcinomas. Earlier excellent experimental evidence has linked these two human tumors...

Burkitt's Tumors Contain Particles Encapsulating RNA-Instructed DNA Polymerase and High Molecular Weight Virus-Related RNA

Kufe, D., Magrath, I. T., Ziegler, J. L., Spiegelman, S.

Burkitt's lymphomas, linked by previous studies with the DNA-containing Epstein-Barr virus, contain an RNA related in sequence to that of Rauscher leukemia virus. The present study establishes that...

Determination of Natural Host Taxonomy of RNA Tumor Viruses by Molecular Hybridization: Application to RD-114, a Candidate Human Virus*

Ruprecht, Ruth M., Goodman, N. C., Spiegelman, S.

The lysogenic bacteriophages and the RNA tumor viruses have in common the ability to add their genetic information to the genome of their host cells. The biological similarity extends further; data...

Decreased Globin Messenger RNA in Thalassemia Detected by Molecular Hybridization

Kacian, D. L., Gambino, R., Dow, L. W., Grossbard, E., Natta, C., Ramirez, F., ...

In previous studies of patients with β thalassemia, mRNA extracted from reticulocytes in peripheral blood when added to cell-free systems reproduces the deficient β-chain synthesis characteristic...

Further Evidence for Oncornaviruses in Human Milk: The Production of Cores

Feldman, Stuart P., Schlom, J., Spiegelman, S.

Cores, or nucleoids, have been isolated from particles in human milk. The cores have a density of 1.26-1.27 g/ml and contain a 60-70S RNA in association with an RNA-instructed DNA polymerase. The...

Sequence Homology Between the RNA of Mason-Pfizer Monkey Virus and the RNA of Human Malignant Breast Tumors

Colcher, David, Spiegelman, S., Schlom, Jeffrey

Radioactive DNA ([3H]cDNA) complementary to the RNA of Mason-Pfizer monkey virus was used in molecular hybridization experiments to demonstrate sequence homology between its viral RNA and RNA of...

ENZYME FORMATION IN PROTOPLASTS OF BACILLUS MEGATERIUM*

Landman, O. E., Spiegelman, S.

On the Cytoplasmic Nature of “Long-Term Adaptation” in Yeast*

Spiegelman, S., Sussman, Racquel Rotman, Pinska, E.

A Single-Cell Analysis of the Transmission of Enzyme-Forming Capacity in Yeast*

Spiegelman, S., DeLorenzo, W. F., Campbell, A. M.

Substrate Stabilization of Enzyme-Forming Capacity During the Segregation of a Heterozygote*

Spiegelman, S., DeLorenzo, W. F.

Mechanism of Enzymatic Adaptation in Genetically Controlled Yeast Populations

Spiegelman, S., Lindegren, Carl C., Hedgecock, L.

Mendelian Inheritance of Adaptive Enzymes*

Lindegren, Carl C., Spiegelman, S., Lindegren, Gertrude

Maintenance and Increase of a Genetic Character by a Substrate-Cytoplasmic Interaction in the Absence of the Specific Gene*

Spiegelman, S., Lindegren, Carl C., Lindegren, Gertrude

On the Redundancy of DNA Complementary to Amino Acid Transfer RNA and Its Absence from the Nucleolar Organizer Region of DROSOPHILA MELANOGASTER

Ritossa, F. M., Atwood, K. C., Spiegelman, S.

A Molecular Explanation of the Bobbed Mutants of Drosophila as Partial Deficiencies of "Ribosomal" DNA

Ritossa, F. M., Atwood, K. C., Spiegelman, S.

Size Distribution of "Informational" RNA

Sagik, B. P., Green, M. H., Hayashi, M., Spiegelman, S.

Previous investigations suggested that the size of “informational” or “messenger” RNA was confined to sedimentation rates lying between 8 and 14S. These involved procedures permitting...

Chromatographic Separation of Annealed and Enzymatically Synthesized RNA-DNA Hybrids

Hayashi, Marie N., Hayashi, M., Spiegelman, S.

A procedure is described for the chromatographic detection and isolation of DNA-RNA hybrids on columns of methylated albumin coated on kieselguhr (MAK). Its use is illustrated with both annealed and...

A Chemical and Physical Characterization of Echinoid RNA during Early Embryogenesis

Slater, D. W., Spiegelman, S.

The properties of the major RNA components prepared from Arbacia punctulata have been characterized by sedimentation in sucrose gradients and analysis of base composition. Comparison of the...

MAXIMA IN RATE-CONCENTRATION CURVES AND THEIR RELATION TO THE STRUCTURAL ASPECTS OF CELLULAR METABOLISM

Spiegelman, S., Reiner, J. M.

1. Reproducible maxima are exhibited in the rate-concentration curves obtained by studying the effects of ethyl- and phenylurethanes on regeneration rates of hydranths in Tubularia. 2. The general...

THE RELATION OF ENZYMATIC ADAPTATION TO THE METABOLISM OF ENDOGENOUS AND EXOGENOUS SUBSTRATES

Spiegelman, S., Reiner, John M., Cohnberg, Rosellen

The source of energy for enzymatic adaptation has been investigated. Aerobically, it is found that the endogenous carbohydrate reserves may be used as such a source. In cells depleted of their...

THE PREADAPTIVE OXIDATION OF GALACTOSE BY YEAST

Reiner, John M., Spiegelman, S.

A preadaptive purely aerobic utilization of galactose by yeast cells has been demonstrated. Hence, the adaptation by yeast to galactose is not to its utilization per se, but specifically to its...

THE FORMATION AND STABILIZATION OF AN ADAPTIVE ENZYME IN THE ABSENCE OF ITS SUBSTRATE

Spiegelman, S., Reiner, John M.

It is shown that various substrates accelerate the disappearance of an adaptive enzyme when its own substrate has been removed from the medium. The order of effectiveness of such substrates appears...

INTERACTIONS BETWEEN ENZYME-FORMING SYSTEMS DURING ADAPTATION

Spiegelman, S., Dunn, Reba

Experiments on enzymatic adaptations in yeast to galactose and maltose under various conditions are examined. The pertinent facts established may be summarized as follows:— 1. The presence of...

ENZYME FORMATION IN NON-VIABLE CELLS

Baron, L. S., Spiegelman, S., Quastler, H.

An attempt has been made to find conditions which would completely dissociate viability from capacity to synthesize enzymes in yeast cells. The only lethal agent or condition found which yielded...

Problems of an RNA Genome Operating in a DNA-Dominated Biological Universe

Spiegelman, S., Haruna, I.

A note on steady states and the weber-fechner law

S. Spiegelman, J. Reiner