Samuel L. Stanley

Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) as a target for anti-amoebic agents (2004)

Espinosa, Avelina, Clark, David, Stanley, Samuel L.

Objectives: The current use of metronidazole as an anti-amoebic agent causes significant side-effects. The purpose of this study was to identify alternative compounds with which to treat amoebiasis....

Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) as a target for anti-amoebic agents (2004)

Espinosa, Avelina, Clark, David, Stanley, Samuel L.

Objectives: The current use of metronidazole as an anti-amoebic agent causes significant side-effects. The purpose of this study was to identify alternative compounds with which to treat amoebiasis....

Innate Immunity to Amebic Liver Abscess Is Dependent on Gamma Interferon and Nitric Oxide in a Murine Model of Disease

Seydel, Karl B., Smith, Sara J., Stanley, Samuel L.

Evidence from in vitro studies suggests that gamma interferon (IFN-γ) and nitric oxide (NO) are important in host defense against the protozoan parasite Entamoeba histolytica. We used SCID mice with...

Shigella Infection in a SCID Mouse-Human Intestinal Xenograft Model: Role for Neutrophils in Containing Bacterial Dissemination in Human Intestine

Zhang, Zhi, Jin, Lingling, Champion, Gretchen, Seydel, Karl B., Stanley, Samuel L.

Shigellae infect human intestine and cause intense inflammation and destruction of colonic and rectal mucosa. To model the interactions of shigella with human intestine in vivo, we have studied...

Amebic Infection in the Human Colon Induces Cyclooxygenase-2

Stenson, William F., Zhang, Zhi, Riehl, Terrence, Stanley, Samuel L.

We sought to determine if infection of the colon with Entamoeba histolytica induces the expression of cyclooxygenase-2 and, if it does, to determine the contribution of prostaglandins produced...

Protection of Gerbils from Amebic Liver Abscess by Vaccination with a 25-mer Peptide Derived from the Cysteine-Rich Region of Entamoeba histolytica Galactose-Specific Adherence Lectin

Lotter, Hannelore, Khajawa, Fareed, Stanley, Samuel L., Tannich, Egbert

The protozoan parasite Entamoeba histolytica causes extensive morbidity and mortality through intestinal infection and amebic liver abscess. Here we show that immunization of gerbils with a single...

Blockade of Caspases Inhibits Amebic Liver Abscess Formation in a Mouse Model of Disease

Yan, Le, Stanley, Samuel L.

We looked at the effect of inhibiting caspases on amebic liver abscess in the mouse model of infection. A dose of the pan-caspase inhibitor benzyloxycarbonyl-V-A-D-O-methyl fluoromethyl ketone...

Mucosal Immunogenicity of a Holotoxin-Like Molecule Containing the Serine-Rich Entamoeba histolytica Protein (SREHP) Fused to the A2 Domain of Cholera Toxin

Sultan, Faisal, Jin, Ling-ling, Jobling, Michael G., Holmes, Randall K., Stanley, Samuel L.

One strategy for the induction of mucosal immune responses by oral immunization is to administer the antigen in conjunction with cholera toxin. Cholera toxin consists of one A polypeptide (CTA) which...

Cryptosporidium parvum Infection of Human Intestinal Xenografts in SCID Mice Induces Production of Human Tumor Necrosis Factor Alpha and Interleukin-8

Seydel, Karl B., Zhang, Tonghai, Champion, Gretchen A., Fichtenbaum, Carl, Swanson, Paul E., Tzipori, Saul, ...

The protozoan parasite Cryptosporidium parvum invades intestinal epithelial cells and can cause life-threatening diarrhea in immunocompromised individuals. Despite the clinical importance of this...

Entamoeba histolytica Induces Host Cell Death in Amebic Liver Abscess by a Non-Fas-Dependent, Non-Tumor Necrosis Factor Alpha-Dependent Pathway of Apoptosis

Seydel, Karl B., Stanley, Samuel L.

Amebic liver abscess is characterized by extensive areas of dead hepatocytes that form cavities surrounded by a thin rim of inflammatory cells and few Entamoeba histolytica trophozoites. E....

A Monoclonal Antibody to the Amebic Lipophosphoglycan-Proteophosphoglycan Antigens Can Prevent Disease in Human Intestinal Xenografts Infected with Entamoeba histolytica

Zhang, Zhi, Duchêne, Michael, Stanley, Samuel L.

Entamoeba histolytica trophozoites are covered by lipophosphoglycan-peptidoglycan molecules which may be key virulence factors. We found that pretreatment of severe combined immunodeficient mice...

Tumor Necrosis Factor Alpha Is a Key Mediator of Gut Inflammation Seen in Amebic Colitis in Human Intestine in the SCID Mouse-Human Intestinal Xenograft Model of Disease

Zhang, Zhi, Mahajan, Sajan, Zhang, Xiaochung, Stanley, Samuel L.

We used Entamoeba histolytica infection in human intestinal xenografts to study the roles interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-α) in the pathogenesis of amebic colitis. We found...

Expression of Amoebapores Is Required for Full Expression of Entamoeba histolytica Virulence in Amebic Liver Abscess but Is Not Necessary for the Induction of Inflammation or Tissue Damage in Amebic Colitis

Zhang, Xiaochun, Zhang, Zhi, Alexander, Diane, Bracha, Rivka, Mirelman, David, Stanley, Samuel L.

Entamoeba histolytica trophozoites produce amoebapores, a family of small amphipathic peptides capable of insertion into bacterial or eukaryotic membranes and causing cellular lysis. Recently, E....

Identification of a family of Bsp-A like surface proteins of Entamoeba histolytica with novel leucine rich repeats.

Davis, Paul H., Zhang, Zhi, Chen, Minghe, Zhang, Xiaochun, Chakraborty, Subhra, Stanley, Samuel L.

Leucine rich repeats serve as recognition motifs for surface proteins from bacteria and eukaryotes. The BspA protein from Bacteroides forsythus mediates bacterial binding to fibronectin and contains...

Innate Immunity to Amebic Liver Abscess Is Dependent on Gamma Interferon and Nitric Oxide in a Murine Model of Disease

Seydel, Karl B., Smith, Sara J., Stanley, Samuel L.

Evidence from in vitro studies suggests that gamma interferon (IFN-γ) and nitric oxide (NO) are important in host defense against the protozoan parasite Entamoeba histolytica. We used SCID mice with...

Shigella Infection in a SCID Mouse-Human Intestinal Xenograft Model: Role for Neutrophils in Containing Bacterial Dissemination in Human Intestine

Zhang, Zhi, Jin, Lingling, Champion, Gretchen, Seydel, Karl B., Stanley, Samuel L.

Shigellae infect human intestine and cause intense inflammation and destruction of colonic and rectal mucosa. To model the interactions of shigella with human intestine in vivo, we have studied...

Amebic Infection in the Human Colon Induces Cyclooxygenase-2

Stenson, William F., Zhang, Zhi, Riehl, Terrence, Stanley, Samuel L.

We sought to determine if infection of the colon with Entamoeba histolytica induces the expression of cyclooxygenase-2 and, if it does, to determine the contribution of prostaglandins produced...

Protection of Gerbils from Amebic Liver Abscess by Vaccination with a 25-mer Peptide Derived from the Cysteine-Rich Region of Entamoeba histolytica Galactose-Specific Adherence Lectin

Lotter, Hannelore, Khajawa, Fareed, Stanley, Samuel L., Tannich, Egbert

The protozoan parasite Entamoeba histolytica causes extensive morbidity and mortality through intestinal infection and amebic liver abscess. Here we show that immunization of gerbils with a single...

Blockade of Caspases Inhibits Amebic Liver Abscess Formation in a Mouse Model of Disease

Yan, Le, Stanley, Samuel L.

We looked at the effect of inhibiting caspases on amebic liver abscess in the mouse model of infection. A dose of the pan-caspase inhibitor benzyloxycarbonyl-V-A-D-O-methyl fluoromethyl ketone...

Mucosal Immunogenicity of a Holotoxin-Like Molecule Containing the Serine-Rich Entamoeba histolytica Protein (SREHP) Fused to the A2 Domain of Cholera Toxin

Sultan, Faisal, Jin, Ling-ling, Jobling, Michael G., Holmes, Randall K., Stanley, Samuel L.

One strategy for the induction of mucosal immune responses by oral immunization is to administer the antigen in conjunction with cholera toxin. Cholera toxin consists of one A polypeptide (CTA) which...

Cryptosporidium parvum Infection of Human Intestinal Xenografts in SCID Mice Induces Production of Human Tumor Necrosis Factor Alpha and Interleukin-8

Seydel, Karl B., Zhang, Tonghai, Champion, Gretchen A., Fichtenbaum, Carl, Swanson, Paul E., Tzipori, Saul, ...

The protozoan parasite Cryptosporidium parvum invades intestinal epithelial cells and can cause life-threatening diarrhea in immunocompromised individuals. Despite the clinical importance of this...

Entamoeba histolytica Induces Host Cell Death in Amebic Liver Abscess by a Non-Fas-Dependent, Non-Tumor Necrosis Factor Alpha-Dependent Pathway of Apoptosis

Seydel, Karl B., Stanley, Samuel L.

Amebic liver abscess is characterized by extensive areas of dead hepatocytes that form cavities surrounded by a thin rim of inflammatory cells and few Entamoeba histolytica trophozoites. E....

A Monoclonal Antibody to the Amebic Lipophosphoglycan-Proteophosphoglycan Antigens Can Prevent Disease in Human Intestinal Xenografts Infected with Entamoeba histolytica

Zhang, Zhi, Duchêne, Michael, Stanley, Samuel L.

Entamoeba histolytica trophozoites are covered by lipophosphoglycan-peptidoglycan molecules which may be key virulence factors. We found that pretreatment of severe combined immunodeficient mice...

Tumor Necrosis Factor Alpha Is a Key Mediator of Gut Inflammation Seen in Amebic Colitis in Human Intestine in the SCID Mouse-Human Intestinal Xenograft Model of Disease

Zhang, Zhi, Mahajan, Sajan, Zhang, Xiaochung, Stanley, Samuel L.

We used Entamoeba histolytica infection in human intestinal xenografts to study the roles interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-α) in the pathogenesis of amebic colitis. We found...

Expression of Amoebapores Is Required for Full Expression of Entamoeba histolytica Virulence in Amebic Liver Abscess but Is Not Necessary for the Induction of Inflammation or Tissue Damage in Amebic Colitis

Zhang, Xiaochun, Zhang, Zhi, Alexander, Diane, Bracha, Rivka, Mirelman, David, Stanley, Samuel L.

Entamoeba histolytica trophozoites produce amoebapores, a family of small amphipathic peptides capable of insertion into bacterial or eukaryotic membranes and causing cellular lysis. Recently, E....

Use of Recombinant Entamoeba histolytica Cysteine Proteinase 1 To Identify a Potent Inhibitor of Amebic Invasion in a Human Colonic Model▿

Meléndez-López, Samuel G., Herdman, Scott, Hirata, Ken, Choi, Min-Ho, Choe, Youngchool, Craik, Charles, ...

Cysteine proteinases are key virulence factors of the protozoan parasite Entamoeba histolytica. We have shown that cysteine proteinases play a central role in tissue invasion and disruption of host...

Identification of an Epitope on the Entamoeba histolytica 170-kD Lectin Conferring Antibody-mediated Protection against Invasive Amebiasis

Lotter, Hannelore, Zhang, Tonghai, Seydel, Karl B., Stanley, Samuel L., Tannich, Egbert

The emergence of multidrug-resistant organisms and the failure to eradicate infection by a number of important pathogens has led to increased efforts to develop vaccines to prevent infectious...

Protection against Invasive Amebiasis by a Single Monoclonal Antibody Directed against a Lipophosphoglycan Antigen Localized on the Surface of Entamoeba histolytica

Marinets, Alexandra, Zhang, Tonghai, Guillén, Nancy, Gounon, Pierre, Bohle, Barbara, Vollmann, Ute, ...

A panel of monoclonal antibodies was raised from mice immunized with a membrane preparation from Entamoeba histolytica, the pathogenic species causing invasive amebiasis in humans. Antibody EH5 gave...

Virulent Shigella flexneri subverts the host innate immune response through manipulation of antimicrobial peptide gene expression

Sperandio, Brice, Regnault, Béatrice, Guo, Jianhua, Zhang, Zhi, Stanley, Samuel L., Sansonetti, Philippe J., ...

Antimicrobial factors are efficient defense components of the innate immunity, playing a crucial role in the intestinal homeostasis and protection against pathogens. In this study, we report that...

Proteomic Comparison of Entamoeba histolytica and Entamoeba dispar and the Role of E. histolytica Alcohol Dehydrogenase 3 in Virulence

Davis, Paul H., Chen, Minghe, Zhang, Xiaochun, Clark, C. Graham, Townsend, R. Reid, Stanley, Samuel L.

The protozoan intestinal parasite Entamoeba histolytica infects millions of people worldwide and is capable of causing amebic dysentery and amebic liver abscess. The closely related species Entamoeba...