Samuel W. Cushman

Proteomic characterization of thiazolidinedione regulation of obese adipose secretome in Zucker obese rats (2009)

Chen, Xiaoli, Hunt, Desmond, Cushman, Samuel W., Hess, Sonja

Signaling molecules released by adipose tissue have been implicated in inflammation, adipocyte dysfunction and systemic insulin resistance. In this study, we used 2-D LC-MS/MS and quantitative...

The Export of Major Histocompatibility Complex Class I Molecules from the Endoplasmic Reticulum of Rat Brown Adipose Cells Is Acutely Stimulated by Insulin

Malide, Daniela, Yewdell, Jonathan W., Bennink, Jack R., Cushman, Samuel W.

Major histocompatibility complex class I (MHC-I) molecules have been implicated in several nonimmunological functions including the regulation and intracellular trafficking of the insulin-responsive...

Studies of Human Adipose Tissue ADIPOSE CELL SIZE AND NUMBER IN NONOBESE AND OBESE PATIENTS

Salans, Lester B., Cushman, Samuel W., Weismann, Rodger E.

The cellular character of the adipose tissue of 21 nonobese and 78 obese patients has been examined. Adipose cell size (lipid per cell) was determined in three different subcutaneous and deep fat...

Mechanism of Insulin-resistant Glucose Transport Activity in the Enlarged Adipose Cell of the Aged, Obese Rat: RELATIVE DEPLETION OF INTRACELLULAR GLUCOSE TRANSPORT SYSTEMS

Hissin, Paul J., Foley, James E., Wardzala, Lawrence J., Karnieli, Eddy, Simpson, Ian A., Salans, Lester B., ...

The effects of increasing cell size on glucose transport activity and metabolism and on the concentrations of glucose transport systems in both the plasma and low density microsomal membranes in...

The Export of Major Histocompatibility Complex Class I Molecules from the Endoplasmic Reticulum of Rat Brown Adipose Cells Is Acutely Stimulated by Insulin

Malide, Daniela, Yewdell, Jonathan W., Bennink, Jack R., Cushman, Samuel W.

Major histocompatibility complex class I (MHC-I) molecules have been implicated in several nonimmunological functions including the regulation and intracellular trafficking of the insulin-responsive...

Studies of Human Adipose Tissue ADIPOSE CELL SIZE AND NUMBER IN NONOBESE AND OBESE PATIENTS

Salans, Lester B., Cushman, Samuel W., Weismann, Rodger E.

The cellular character of the adipose tissue of 21 nonobese and 78 obese patients has been examined. Adipose cell size (lipid per cell) was determined in three different subcutaneous and deep fat...

Mechanism of Insulin-resistant Glucose Transport Activity in the Enlarged Adipose Cell of the Aged, Obese Rat: RELATIVE DEPLETION OF INTRACELLULAR GLUCOSE TRANSPORT SYSTEMS

Hissin, Paul J., Foley, James E., Wardzala, Lawrence J., Karnieli, Eddy, Simpson, Ian A., Salans, Lester B., ...

The effects of increasing cell size on glucose transport activity and metabolism and on the concentrations of glucose transport systems in both the plasma and low density microsomal membranes in...

Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Use Different Receptor Complexes To Enter T Cells

Jones, Kathryn S., Fugo, Kazunori, Petrow-Sadowski, Cari, Huang, Ying, Bertolette, Daniel C., Lisinski, Ivonne, ...

Studies using adherent cell lines have shown that glucose transporter-1 (GLUT-1) can function as a receptor for human T-cell leukemia virus type 1 (HTLV). In primary CD4+ T cells, heparan sulfate...

GLUT1 Is Not the Primary Binding Receptor but Is Associated with Cell-to-Cell Transmission of Human T-Cell Leukemia Virus Type 1▿

Takenouchi, Norihiro, Jones, Kathryn S., Lisinski, Ivonne, Fugo, Kazunori, Yao, Karen, Cushman, Samuel W., ...

GLUT1 has recently been suggested to be a binding receptor for human T-cell leukemia virus type 1 (HTLV-1). We used a novel, short-term assay to define the role of GLUT1 in cell-to-cell transmission....

Insulin stimulates the halting, tethering, and fusion of mobile GLUT4 vesicles in rat adipose cells

Lizunov, Vladimir A., Matsumoto, Hideko, Zimmerberg, Joshua, Cushman, Samuel W., Frolov, Vadim A.

Glucose transport in adipose cells is regulated by changing the distribution of glucose transporter 4 (GLUT4) between the cell interior and the plasma membrane (PM). Insulin shifts this distribution...

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ADIPOSE CELL : III. Effects of Bovine Serum Albumin on the Metabolism of Glucose and the Release of Nonesterified Fatty Acids and Glycerol by the Isolated Adipose Cell

Cushman, Samuel W., Rizack, Martin A.

Serum albumin stimulates the uptake of U-glucose-14C and the incorporation of 14C-counts into triglyceride glycerol and inhibits the incorporation of 14C-counts into triglyceride fatty acids by...

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ADIPOSE CELL : I. Ultrastructure of the Isolated Adipose Cell

Cushman, Samuel W.

A method is described for preparing isolated rat adipose cells for electron microscopy. The ultrastructure of such cells and their production of 14CO2 from U-glucose-14C were studied simultaneously...

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ADIPOSE CELL : II. Pinocytosis and Factors Influencing Its Activity in the Isolated Adipose Cell

Cushman, Samuel W.

Pinocytic activity in the adipose cell has been examined by measuring the uptake of colloidal gold. Pinocytic activity occurs in the isolated adipose cell under all experimental conditions; a portion...

Analysis of GLUT4 Distribution in Whole Skeletal Muscle Fibers: Identification of Distinct Storage Compartments That Are Recruited by Insulin and Muscle Contractions

Ploug, Thorkil, Van Deurs, Bo, Ai, Hua, Cushman, Samuel W., Ralston, Evelyn

The effects of insulin stimulation and muscle contractions on the subcellular distribution of GLUT4 in skeletal muscle have been studied on a preparation of single whole fibers from the rat soleus....

Tankyrase 1 and Tankyrase 2 Are Essential but Redundant for Mouse Embryonic Development

Chiang, Y. Jeffrey, Hsiao, Susan J., Yver, Dena, Cushman, Samuel W., Tessarollo, Lino, Smith, Susan, ...

Tankyrases are proteins with poly(ADP-ribose) polymerase activity. Human tankyrases post-translationally modify multiple proteins involved in processes including maintenance of telomere length,...

Hypertrophy and/or Hyperplasia: Dynamics of Adipose Tissue Growth

Jo, Junghyo, Gavrilova, Oksana, Pack, Stephanie, Jou, William, Mullen, Shawn, Sumner, Anne E., ...

Adipose tissue grows by two mechanisms: hyperplasia (cell number increase) and hypertrophy (cell size increase). Genetics and diet affect the relative contributions of these two mechanisms to the...

A common trafficking route for GLUT4 in cardiomyocytes in response to insulin, contraction and energy-status signalling

Fazakerley, Daniel J., Lawrence, Scott P., Lizunov, Vladimir A., Cushman, Samuel W., Holman, Geoffrey D.

A new mouse model has been developed to study the localisation and trafficking of the glucose transporter GLUT4 in muscle. The mouse line has specific expression of a GFP and HA-epitope-tagged...