Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly (2006)
Nolwenn Jouvenet, Cameron Bess, Marc C. Johnson, Cesar A. Virgen, Sanford M. Simon, ...
Recently proposed models that have gained wide acceptance posit that HIV-1 virion morphogenesis is initiated by targeting the major structural protein (Gag) to late endosomal membranes. Thereafter,...
Synaptotagmin VII Restricts Fusion Pore Expansion during Lysosomal Exocytosis (2004)
Jyoti K. Jaiswal, Sabyasachi Chakrabarti, Norma W. Andrews, Sanford M. Simon
Lysosomes only partially release their contents in wild-type mouse embryonic fibroblasts (MEFs); but in MEFs in which synaptotagmin VII has been deleted, lysosomes fuse completely.
Synaptotagmin VII Restricts Fusion Pore Expansion during Lysosomal Exocytosis (2004)
Jyoti K. Jaiswal, Sabyasachi Chakrabarti, Norma W. Andrews, Sanford M. Simon
Synaptotagmin is considered a calcium-dependent trigger for regulated exocytosis. We examined the role of synaptotagmin VII (Syt VII) in the calcium-dependent exocytosis of individual lysosomes in...
Imaging constitutive exocytosis with total internal reflection fluorescence microscopy (2000)
Jan Schmoranzer, Mark Goulian, Dan Axelrod, Sanford M. Simon
Abstract. Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma...
What Drives the Translocation of Proteins? (1992)
Sanford M. Simon, Charles S. Peskin, George F. Oster
We propose that protein translocation across lipid bilayers is driven by biased random thermal motion. This "Brownian Ratchet" mechanism rests on the assumption that there is a chemical...
Tamoxifen inhibits acidification in cells independent of the estrogen receptor
Altan, Nihal, Chen, Yu, Schindler, Melvin, Simon, Sanford M.
Tamoxifen has been reported to have numerous physiological effects that are independent of the estrogen receptor, including sensitization of resistant tumor cells to many chemotherapeutic agents....
Assembling filamentous phage occlude pIV channels
Marciano, Denise K., Russel, Marjorie, Simon, Sanford M.
Filamentous phage f1 is exported from its Escherichia coli host without killing the bacterial cell. Phage-encoded protein pIV, which is required for phage assembly and secretion, forms large highly...
Lampson, Michael A., Schmoranzer, Jan, Zeigerer, Anja, Simon, Sanford M., McGraw, Timothy E.
In several cell types, specific membrane proteins are retained intracellularly and rapidly redistributed to the surface in response to stimulation. In fat and muscle, the GLUT4 glucose transporter is...
Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line
Kahl, Barbara C., Goulian, Mark, Van Wamel, Willem, Herrmann, Mathias, Simon, Sanford M., Kaplan, Gilla, ...
Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may...
Role of Microtubules in Fusion of Post-Golgi Vesicles to the Plasma MembraneV⃞
Schmoranzer, Jan, Simon, Sanford M.
Biosynthetic cargo is transported away from the Golgi in vesicles via microtubules. In the cell periphery the vesicles are believed to engage actin and then dock to fusion sites at the plasma...
Subcellular Localization and Activity of Multidrug Resistance Proteins
Rajagopal, Asha, Simon, Sanford M.
The multidrug resistance (MDR) phenotype is associated with the overexpression of members of the ATP-binding cassette family of proteins. These MDR transporters are expressed at the plasma membrane,...
Imaging single membrane fusion events mediated by SNARE proteins
Fix, Marina, Melia, Thomas J., Jaiswal, Jyoti K., Rappoport, Joshua Z., You, Daoqi, Söllner, Thomas H., ...
Using total internal reflection fluorescence microscopy, we have developed an assay to monitor individual fusion events between proteoliposomes containing vesicle soluble N-ethylmaleimide-sensitive...
Synaptotagmin VII Restricts Fusion Pore Expansion during Lysosomal Exocytosis
Jaiswal, Jyoti K, Chakrabarti, Sabyasachi, Andrews, Norma W, Simon, Sanford M
Synaptotagmin is considered a calcium-dependent trigger for regulated exocytosis. We examined the role of synaptotagmin VII (Syt VII) in the calcium-dependent exocytosis of individual lysosomes in...
Analysis of the AP-2 Adaptor Complex and Cargo During Clathrin-Mediated Endocytosis
Rappoport, Joshua Z., Benmerah, Alexandre, Simon, Sanford M.
Previously, we reported that the hetero-tetrameric adaptor complex AP-2 co-localizes with the static population of clathrin spots, whereas it is excluded from clathrin spots that disappear from the...
Receptor-mediated glutamate release from volume sensitive channels in astrocytes
Takano, Takahiro, Kang, Jian, Jaiswal, Jyoti K., Simon, Sanford M., Lin, Jane H.-C., Yu, Yufei, ...
Several lines of work have shown that astrocytes release glutamate in response to receptor activation, which results in a modulation of local synaptic activity. Astrocytic glutamate release is...
Tamoxifen inhibits acidification in cells independent of the estrogen receptor
Altan, Nihal, Chen, Yu, Schindler, Melvin, Simon, Sanford M.
Tamoxifen has been reported to have numerous physiological effects that are independent of the estrogen receptor, including sensitization of resistant tumor cells to many chemotherapeutic agents....
Assembling filamentous phage occlude pIV channels
Marciano, Denise K., Russel, Marjorie, Simon, Sanford M.
Filamentous phage f1 is exported from its Escherichia coli host without killing the bacterial cell. Phage-encoded protein pIV, which is required for phage assembly and secretion, forms large highly...
Lampson, Michael A., Schmoranzer, Jan, Zeigerer, Anja, Simon, Sanford M., McGraw, Timothy E.
In several cell types, specific membrane proteins are retained intracellularly and rapidly redistributed to the surface in response to stimulation. In fat and muscle, the GLUT4 glucose transporter is...
Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line
Kahl, Barbara C., Goulian, Mark, Van Wamel, Willem, Herrmann, Mathias, Simon, Sanford M., Kaplan, Gilla, ...
Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may...
Role of Microtubules in Fusion of Post-Golgi Vesicles to the Plasma MembraneV⃞
Schmoranzer, Jan, Simon, Sanford M.
Biosynthetic cargo is transported away from the Golgi in vesicles via microtubules. In the cell periphery the vesicles are believed to engage actin and then dock to fusion sites at the plasma...
Subcellular Localization and Activity of Multidrug Resistance Proteins
Rajagopal, Asha, Simon, Sanford M.
The multidrug resistance (MDR) phenotype is associated with the overexpression of members of the ATP-binding cassette family of proteins. These MDR transporters are expressed at the plasma membrane,...
Imaging single membrane fusion events mediated by SNARE proteins
Fix, Marina, Melia, Thomas J., Jaiswal, Jyoti K., Rappoport, Joshua Z., You, Daoqi, Söllner, Thomas H., ...
Using total internal reflection fluorescence microscopy, we have developed an assay to monitor individual fusion events between proteoliposomes containing vesicle soluble N-ethylmaleimide-sensitive...
Synaptotagmin VII Restricts Fusion Pore Expansion during Lysosomal Exocytosis
Jaiswal, Jyoti K, Chakrabarti, Sabyasachi, Andrews, Norma W, Simon, Sanford M
Synaptotagmin is considered a calcium-dependent trigger for regulated exocytosis. We examined the role of synaptotagmin VII (Syt VII) in the calcium-dependent exocytosis of individual lysosomes in...
Receptor-mediated glutamate release from volume sensitive channels in astrocytes
Takano, Takahiro, Kang, Jian, Jaiswal, Jyoti K., Simon, Sanford M., Lin, Jane H.-C., Yu, Yufei, ...
Several lines of work have shown that astrocytes release glutamate in response to receptor activation, which results in a modulation of local synaptic activity. Astrocytic glutamate release is...
Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly
Jouvenet, Nolwenn, Neil, Stuart J. D, Bess, Cameron, Johnson, Marc C, Virgen, Cesar A, Simon, Sanford M, ...
Recently proposed models that have gained wide acceptance posit that HIV-1 virion morphogenesis is initiated by targeting the major structural protein (Gag) to late endosomal membranes. Thereafter,...
Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes
Jaiswal, Jyoti K., Fix, Marina, Takano, Takahiro, Nedergaard, Maiken, Simon, Sanford M.
Optical imaging of individual vesicle exocytosis is providing new insights into the mechanism and regulation of secretion by cells. To study calcium-triggered secretion from astrocytes, we used...
Defective Acidification in Human Breast Tumor Cells and Implications for Chemotherapy
Altan, Nihal, Chen, Yu, Schindler, Melvin, Simon, Sanford M.
Multidrug resistance (MDR) is a significant problem in the treatment of cancer. Chemotherapeutic drugs distribute through the cyto- and nucleoplasm of drug-sensitive cells but are excluded from the...
Jaiswal, Jyoti K., Andrews, Norma W., Simon, Sanford M.
Similar to its role in secretory cells, calcium triggers exocytosis in nonsecretory cells. This calcium-dependent exocytosis is essential for repair of membrane ruptures. Using total internal...
In Situ Biochemical Demonstration That P-Glycoprotein Is a Drug Efflux Pump with Broad Specificity
While P-glycoprotein (Pgp) is the most studied protein involved in resistance to anti-cancer drugs, its mechanism of action is still under debate. Studies of Pgp have used cell lines selected with...
Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy
Schmoranzer, Jan, Goulian, Mark, Axelrod, Dan, Simon, Sanford M.
Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma membrane. The...