Sanjai Kumar

Measuring the Effects of an Ever-Changing Environment on Malaria Control (2006)

McCutchan, Thomas F., Grim, K. C., Li, Jun, Weiss, Walter, Rathore, Darmendar, Sullivan, Margery, ...

The effectiveness of malaria control measures depends not only on the potency of the control measures themselves but also upon the influence of variables associated with the environment....

CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine (2006)

Kumar, Sanjai, Jones, Trevor R., Oakley, Miranda S., Zheng, Hong, Kuppusamy, Shanmuga P., Taye, Alem, ...

Unmethylated CpG dinucleotide motifs present in bacterial genomes or synthetic oligodeoxynucleotides (ODNs) serve as strong immunostimulatory agents in mice, monkeys and humans. We determined the...

Immunogenicity of Well-Characterized Synthetic Plasmodium Falciparum Multiple Antigen Peptide Conjugates (2002)

Joshi, Manju B., Gam, Albert A., Boykins, Robert A., Kumar, Sanjai, Sacci, John

Given the emerging difficulties with malaria drug resistance and vector control, as well as the persistent lack of an effective vaccine, new malaria vaccine development strategies are needed. We used...

Immunogenicity and Efficacy in Aotus Monkeys of Four Recombinant Plasmodium falciparum Vaccines in Multiple Adjuvant Formulations Based on the 19-Kilodalton C Terminus of Merozoite Surface Protein 1

Kumar, Sanjai, Collins, William, Egan, Andrea, Yadava, Anjali, Garraud, Olivier, Blackman, Michael J., ...

The immunogenicity and protective efficacy of four versions of recombinant C-terminal 19-kDa epidermal growth factor-like region of the major surface protein 1 (rMSP119) of Plasmodium falciparum was...

HLA-A*01-Restricted Cytotoxic T-Lymphocyte Epitope from the Plasmodium falciparum Circumsporozoite Protein

Kumar, Anita, Kumar, Sanjai, Le, Thong P., Southwood, Scott, Sidney, John, Cohen, Joe, ...

Here, we report the identification of a novel CD8+ cytotoxic T-lymphocyte epitope on the Plasmodium falciparum circumsporozoite protein (3D7; amino acids 310 to 319 [EPSDKHIKEY]) that is restricted...

Immunogenicity of Well-Characterized Synthetic Plasmodium falciparum Multiple Antigen Peptide Conjugates

Joshi, Manju B., Gam, Albert A., Boykins, Robert A., Kumar, Sanjai, Sacci, John, Hoffman, Stephen L., ...

Given the emerging difficulties with malaria drug resistance and vector control, as well as the persistent lack of an effective vaccine, new malaria vaccine development strategies are needed. We used...

Multistage Multiantigen Heterologous Prime Boost Vaccine for Plasmodium knowlesi Malaria Provides Partial Protection in Rhesus Macaques

Rogers, William O., Baird, J. Kevin, Kumar, Anita, Tine, John A., Weiss, Walter, Aguiar, João C., ...

A nonhuman primate model for malaria vaccine development allowing reliable, stringent sporozoite challenge and evaluation of both cellular and antibody responses is needed. We therefore constructed a...

Codon Optimization of Gene Fragments Encoding Plasmodium falciparum Merzoite Proteins Enhances DNA Vaccine Protein Expression and Immunogenicity in Mice

Narum, David L., Kumar, Sanjai, Rogers, William O., Fuhrmann, Steven R., Liang, Hong, Oakley, Miranda, ...

In contrast to conventional vaccines, DNA and other subunit vaccines exclusively utilize host cell molecules for transcription and translation of proteins. The adenine plus thymine content of...

Protection of Rhesus Macaques against Lethal Plasmodium knowlesi Malaria by a Heterologous DNA Priming and Poxvirus Boosting Immunization Regimen

Rogers, William O., Weiss, Walter R., Kumar, Anita, Aguiar, João C., Tine, John A., Gwadz, Robert, ...

We tested a cytokine-enhanced, multiantigen, DNA priming and poxvirus boosting vaccine regimen for prevention of malaria in the Plasmodium knowlesi-rhesus macaque model system. Animals were primed...

CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine

Kumar, Sanjai, Jones, Trevor R., Oakley, Miranda S., Zheng, Hong, Kuppusamy, Shanmuga P., Taye, Alem, ...

Unmethylated CpG dinucleotide motifs present in bacterial genomes or synthetic oligodeoxynucleotides (ODNs) serve as strong immunostimulatory agents in mice, monkeys and humans. We determined the...

Measuring the Effects of an Ever-Changing Environment on Malaria Control

McCutchan, Thomas F., Grim, K. Christiana, Li, Jun, Weiss, Walter, Rathore, Darmendar, Sullivan, Margery, ...

The effectiveness of malaria control measures depends not only on the potency of the control measures themselves but also upon the influence of variables associated with the environment....

Activity-based probes for protein tyrosine phosphatases

Kumar, Sanjai, Zhou, Bo, Liang, Fubo, Wang, Wei-Qing, Huang, Zhonghui, Zhang, Zhong-Yin

Protein tyrosine phosphatases (PTPs) are involved in the regulation of many aspects of cellular activity including proliferation, differentiation, metabolism, migration, and survival. Given the large...

Genome-Wide Expression Profiling in Malaria Infection Reveals Transcriptional Changes Associated with Lethal and Nonlethal Outcomes†

Schaecher, Kurt, Kumar, Sanjai, Yadava, Anjali, Vahey, Maryanne, Ockenhouse, Christian F.

High-density oligonucleotide microarrays are widely used to study gene expression in cells exposed to a variety of pathogens. This study addressed the global genome-wide transcriptional activation of...

Identification, Cloning, Expression, and Characterization of the Gene for Plasmodium knowlesi Surface Protein Containing an Altered Thrombospondin Repeat Domain

Mahajan, Babita, Jani, Dewal, Chattopadhyay, Rana, Nagarkatti, Rana, Zheng, Hong, Majam, Victoria, ...

Proteins present on the surface of malaria parasites that participate in the process of invasion and adhesion to host cells are considered attractive vaccine targets. Aided by the availability of the...

Immunogenicity and Efficacy in Aotus Monkeys of Four Recombinant Plasmodium falciparum Vaccines in Multiple Adjuvant Formulations Based on the 19-Kilodalton C Terminus of Merozoite Surface Protein 1

Kumar, Sanjai, Collins, William, Egan, Andrea, Yadava, Anjali, Garraud, Olivier, Blackman, Michael J., ...

The immunogenicity and protective efficacy of four versions of recombinant C-terminal 19-kDa epidermal growth factor-like region of the major surface protein 1 (rMSP119) of Plasmodium falciparum was...

HLA-A*01-Restricted Cytotoxic T-Lymphocyte Epitope from the Plasmodium falciparum Circumsporozoite Protein

Kumar, Anita, Kumar, Sanjai, Le, Thong P., Southwood, Scott, Sidney, John, Cohen, Joe, ...

Here, we report the identification of a novel CD8+ cytotoxic T-lymphocyte epitope on the Plasmodium falciparum circumsporozoite protein (3D7; amino acids 310 to 319 [EPSDKHIKEY]) that is restricted...

Immunogenicity of Well-Characterized Synthetic Plasmodium falciparum Multiple Antigen Peptide Conjugates

Joshi, Manju B., Gam, Albert A., Boykins, Robert A., Kumar, Sanjai, Sacci, John, Hoffman, Stephen L., ...

Given the emerging difficulties with malaria drug resistance and vector control, as well as the persistent lack of an effective vaccine, new malaria vaccine development strategies are needed. We used...

Multistage Multiantigen Heterologous Prime Boost Vaccine for Plasmodium knowlesi Malaria Provides Partial Protection in Rhesus Macaques

Rogers, William O., Baird, J. Kevin, Kumar, Anita, Tine, John A., Weiss, Walter, Aguiar, João C., ...

A nonhuman primate model for malaria vaccine development allowing reliable, stringent sporozoite challenge and evaluation of both cellular and antibody responses is needed. We therefore constructed a...

Codon Optimization of Gene Fragments Encoding Plasmodium falciparum Merzoite Proteins Enhances DNA Vaccine Protein Expression and Immunogenicity in Mice

Narum, David L., Kumar, Sanjai, Rogers, William O., Fuhrmann, Steven R., Liang, Hong, Oakley, Miranda, ...

In contrast to conventional vaccines, DNA and other subunit vaccines exclusively utilize host cell molecules for transcription and translation of proteins. The adenine plus thymine content of...

Protection of Rhesus Macaques against Lethal Plasmodium knowlesi Malaria by a Heterologous DNA Priming and Poxvirus Boosting Immunization Regimen

Rogers, William O., Weiss, Walter R., Kumar, Anita, Aguiar, João C., Tine, John A., Gwadz, Robert, ...

We tested a cytokine-enhanced, multiantigen, DNA priming and poxvirus boosting vaccine regimen for prevention of malaria in the Plasmodium knowlesi-rhesus macaque model system. Animals were primed...

CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine

Kumar, Sanjai, Jones, Trevor R., Oakley, Miranda S., Zheng, Hong, Kuppusamy, Shanmuga P., Taye, Alem, ...

Unmethylated CpG dinucleotide motifs present in bacterial genomes or synthetic oligodeoxynucleotides (ODNs) serve as strong immunostimulatory agents in mice, monkeys and humans. We determined the...

Measuring the Effects of an Ever-Changing Environment on Malaria Control

McCutchan, Thomas F., Grim, K. Christiana, Li, Jun, Weiss, Walter, Rathore, Darmendar, Sullivan, Margery, ...

The effectiveness of malaria control measures depends not only on the potency of the control measures themselves but also upon the influence of variables associated with the environment....

Activity-based probes for protein tyrosine phosphatases

Kumar, Sanjai, Zhou, Bo, Liang, Fubo, Wang, Wei-Qing, Huang, Zhonghui, Zhang, Zhong-Yin

Protein tyrosine phosphatases (PTPs) are involved in the regulation of many aspects of cellular activity including proliferation, differentiation, metabolism, migration, and survival. Given the large...

Genome-Wide Expression Profiling in Malaria Infection Reveals Transcriptional Changes Associated with Lethal and Nonlethal Outcomes†

Schaecher, Kurt, Kumar, Sanjai, Yadava, Anjali, Vahey, Maryanne, Ockenhouse, Christian F.

High-density oligonucleotide microarrays are widely used to study gene expression in cells exposed to a variety of pathogens. This study addressed the global genome-wide transcriptional activation of...

Identification, Cloning, Expression, and Characterization of the Gene for Plasmodium knowlesi Surface Protein Containing an Altered Thrombospondin Repeat Domain

Mahajan, Babita, Jani, Dewal, Chattopadhyay, Rana, Nagarkatti, Rana, Zheng, Hong, Majam, Victoria, ...

Proteins present on the surface of malaria parasites that participate in the process of invasion and adhesion to host cells are considered attractive vaccine targets. Aided by the availability of the...

A Novel Chimeric Plasmodium vivax Circumsporozoite Protein Induces Biologically Functional Antibodies That Recognize both VK210 and VK247 Sporozoites▿

Yadava, Anjali, Sattabongkot, Jetsumon, Washington, Michael A., Ware, Lisa A., Majam, Victoria, Zheng, Hong, ...

A successful vaccine against Plasmodium vivax malaria would significantly improve the health and quality of the lives of more than 1 billion people around the world. A subunit vaccine is the only...

Molecular Factors and Biochemical Pathways Induced by Febrile Temperature in Intraerythrocytic Plasmodium falciparum Parasites▿ †

Oakley, Miranda S. M., Kumar, Sanjai, Anantharaman, Vivek, Zheng, Hong, Mahajan, Babita, Haynes, J. David, ...

Intermittent episodes of febrile illness are the most benign and recognized symptom of infection with malaria parasites, although the effects on parasite survival and virulence remain unclear. In...

HDP—A Novel Heme Detoxification Protein from the Malaria Parasite

Jani, Dewal, Nagarkatti, Rana, Beatty, Wandy, Angel, Ross, Slebodnick, Carla, Andersen, John, ...

When malaria parasites infect host red blood cells (RBC) and proteolyze hemoglobin, a unique, albeit poorly understood parasite-specific mechanism, detoxifies released heme into hemozoin (Hz). Here,...

Host Biomarkers and Biological Pathways That Are Associated with the Expression of Experimental Cerebral Malaria in Mice▿ †

Oakley, Miranda S., McCutchan, Thomas F., Anantharaman, Vivek, Ward, Jerrold M., Faucette, Laurence, Erexson, Cindy, ...

Cerebral malaria (CM) is a primary cause of malaria-associated deaths among young African children. Yet no diagnostic tools are available that could be used to predict which of the children infected...

Pathogenic Roles of CD14, Galectin-3, and OX40 during Experimental Cerebral Malaria in Mice

Oakley, Miranda S., Majam, Victoria, Mahajan, Babita, Gerald, Noel, Anantharaman, Vivek, Ward, Jerrold M., ...

An in-depth knowledge of the host molecules and biological pathways that contribute towards the pathogenesis of cerebral malaria would help guide the development of novel prognostics and...