Drosophila PIWI associates with chromatin and interacts directly with HP1a (2007)
Brower-Toland, Brent, Findley, Seth D., Jiang, Ling, Liu, Li, Yin, Hang, Dus, Monica, ...
The interface between cellular systems involving small noncoding RNAs and epigenetic change remains largely unexplored in metazoans. RNA-induced silencing systems have the potential to target...
Shaffer, Christopher D., Cenci, Giovanni, Thompson, Brandi, Stephens, Gena E., Slawson, Elizabeth E., Adu-Wusu, Kwame, ...
Drosophila melanogaster heterochromatin protein 2 (HP2) interacts with heterochromatin protein 1 (HP1). In polytene chromosomes, HP2 and HP1 colocalize at the chromocenter, telomeres, and the small...
Lu, Quinn, Teare, John M., Granok, Howard, Swede, Marci J., Xu, Jenny, Elgin, Sarah C. R.
Previous studies of the Drosophila melanogaster hsp26 gene promoter have demonstrated the importance of a homopurine·homopyrimidine segment [primarily (CT)n·(GA)n] for chromatin structure...
Clark, Robert F., Elgin, Sarah C.R.
The Su(var)205 gene of Drosophila melanogaster encodes heterochromatin protein 1 (HP1), a protein located preferentially within β-heterochromatin. Mutation of this gene has been associated with...
Eissenberg, Joel C., Kimbrell, Deborah A., Fristrom, James W., Elgin, Sarah C.R.
The chromatin structure of the larval cuticle gene cluster at 44D was characterized in embryos from wild-type (Oregon R) and a variant line (27sol;3) of Drosophila melanogaster. A major DNase I...
Cleavage of chromatin with methidiumpropyl-EDTA·iron(II) (1983)
Cartwright, Iain L., Hertzberg, Robert P., Dervan, Peter B., Elgin, Sarah C. R.
Methidiumpropyl-EDTA·iron(II) [MPE·Fe(II)] cleaves double-helical DNA with considerably lower sequence specificity than micrococcal nuclease. Moreover, digestions with MPE·Fe(II) can be performed...
Cartwright, Iain L., Elgin, Sarah C.R.
Limited treatment of Drosophila nuclei with the 1,10-phenanthroline-cuprous complex leads to rapid production of nucleosomal ladders indistinguishable from those obtained by micrococcal nuclease...
Wong, Yuk-Chor, Peter, O'Connell, Rosbash, Michael, Elgin, Sarah C.R.
By using an indirect end-labelling technique for mapping, five DNase I hypersensitive sites have been located in Drosophila melanogaster chromatin at the 5′-end of the gene coding for ribosomal...
Proteins of metaphase chromosomes and interphase chromatin (1980)
Wray, Virginia P., Elgin, Sarah C.R., Wray, Wayne
Metaphase chromosomal and interphase chromatin proteins from cells of two species have been campared by polyacrylamide gel electrophoresis. Consistent, common changes in the quantitative distribution...
Sun, Fang-Lin, Cuaycong, Matthew H., Craig, Carolyn A., Wallrath, Lori L., Locke, John, Elgin, Sarah C. R.
The small fourth chromosome of Drosophila melanogaster (3.5% of the genome) presents a puzzle. Cytological analysis suggests that the bulk of the fourth, including the portion that appears banded in...
Sun, Fang-Lin, Cuaycong, Matthew H., Elgin, Sarah C. R.
We have used line HS-2 of Drosophila melanogaster, carrying a silenced transgene in the pericentric heterochromatin, to investigate in detail the chromatin structure imposed by this environment....
Leibovitch, Boris A., Lu, Quinn, Benjamin, Lawrence R., Liu, Yingyun, Gilmour, David S., Elgin, Sarah C. R.
The upstream regulatory region of the Drosophila melanogaster hsp26 gene includes two DNase I-hypersensitive sites (DH sites) that encompass the critical heat shock elements. This chromatin structure...
Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin
Shaffer, Christopher D., Stephens, Gena E., Thompson, Brandi A., Funches, Levi, Bernat, John A., Craig, Carolyn A., ...
Heterochromatin protein 1 (HP1), first discovered in Drosophila melanogaster, is a highly conserved chromosomal protein implicated in both heterochromatin formation and gene silencing. We report here...
Lu, Quinn, Teare, John M., Granok, Howard, Swede, Marci J., Xu, Jenny, Elgin, Sarah C. R.
Previous studies of the Drosophila melanogaster hsp26 gene promoter have demonstrated the importance of a homopurine·homopyrimidine segment [primarily (CT)n·(GA)n] for chromatin structure formation...
Bednarski, April E, Elgin, Sarah C.R, Pakrasi, Himadri B
This inquiry-based lab is designed around genetic diseases with a focus on protein structure and function. To allow students to work on their own investigatory projects, 10 projects on 10 different...
Flowers, Susan K, Easter, Carla, Holmes, Andrea, Cohen, Brian, Bednarski, April E, Mardis, Elaine R, ...
Sequencing of the human genome has ushered in a new era of biology. The technologies developed to facilitate the sequencing of the human genome are now being applied to the sequencing of other...
Eissenberg, Joel C., Elgin, Sarah C. R.
We have identified and cloned a mutant allele of the small heat shock gene Hsp28 of Drosophila melanogaster. This allele, which we have called Hsp28stl, produces small amounts of a single aberrantly...
Sun, Fang-Lin, Cuaycong, Matthew H., Craig, Carolyn A., Wallrath, Lori L., Locke, John, Elgin, Sarah C. R.
The small fourth chromosome of Drosophila melanogaster (3.5% of the genome) presents a puzzle. Cytological analysis suggests that the bulk of the fourth, including the portion that appears banded in...
Sun, Fang-Lin, Cuaycong, Matthew H., Elgin, Sarah C. R.
We have used line HS-2 of Drosophila melanogaster, carrying a silenced transgene in the pericentric heterochromatin, to investigate in detail the chromatin structure imposed by this environment....
Leibovitch, Boris A., Lu, Quinn, Benjamin, Lawrence R., Liu, Yingyun, Gilmour, David S., Elgin, Sarah C. R.
The upstream regulatory region of the Drosophila melanogaster hsp26 gene includes two DNase I-hypersensitive sites (DH sites) that encompass the critical heat shock elements. This chromatin structure...
Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin
Shaffer, Christopher D., Stephens, Gena E., Thompson, Brandi A., Funches, Levi, Bernat, John A., Craig, Carolyn A., ...
Heterochromatin protein 1 (HP1), first discovered in Drosophila melanogaster, is a highly conserved chromosomal protein implicated in both heterochromatin formation and gene silencing. We report here...
Lu, Quinn, Teare, John M., Granok, Howard, Swede, Marci J., Xu, Jenny, Elgin, Sarah C. R.
Previous studies of the Drosophila melanogaster hsp26 gene promoter have demonstrated the importance of a homopurine·homopyrimidine segment [primarily (CT)n·(GA)n] for chromatin structure formation...
Bednarski, April E, Elgin, Sarah C.R, Pakrasi, Himadri B
This inquiry-based lab is designed around genetic diseases with a focus on protein structure and function. To allow students to work on their own investigatory projects, 10 projects on 10 different...
Eissenberg, Joel C., Elgin, Sarah C. R.
We have identified and cloned a mutant allele of the small heat shock gene Hsp28 of Drosophila melanogaster. This allele, which we have called Hsp28stl, produces small amounts of a single aberrantly...
Flowers, Susan K, Easter, Carla, Holmes, Andrea, Cohen, Brian, Bednarski, April E, Mardis, Elaine R, ...
Sequencing of the human genome has ushered in a new era of biology. The technologies developed to facilitate the sequencing of the human genome are now being applied to the sequencing of other...
Shaffer, Christopher D., Cenci, Giovanni, Thompson, Brandi, Stephens, Gena E., Slawson, Elizabeth E., Adu-Wusu, Kwame, ...
Drosophila melanogaster heterochromatin protein 2 (HP2) interacts with heterochromatin protein 1 (HP1). In polytene chromosomes, HP2 and HP1 colocalize at the chromocenter, telomeres, and the small...
A Distinct Type of Heterochromatin Within Drosophila melanogaster Chromosome 4
Haynes, Karmella A., Gracheva, Elena, Elgin, Sarah C. R.
Studies of transcriptional gene silencing in Drosophila melanogaster suggest that most of chromosome 4 resembles pericentric heterochromatin. However, some modifiers of position-effect variegation,...
Drosophila PIWI associates with chromatin and interacts directly with HP1a
Brower-Toland, Brent, Findley, Seth D., Jiang, Ling, Liu, Li, Yin, Hang, Dus, Monica, ...
The interface between cellular systems involving small noncoding RNAs and epigenetic change remains largely unexplored in metazoans. RNA-induced silencing systems have the potential to target...
An Investigation of Heterochromatin Domains on the Fourth Chromosome of Drosophila melanogaster
Riddle, Nicole C., Leung, Wilson, Haynes, Karmella A., Granok, Howard, Wuller, Jo, Elgin, Sarah C. R.
The banded portion of Drosophila melanogaster chromosome 4 exhibits euchromatic and heterochromatic characteristics. Reminiscent of heterochromatin, it contains a high percentage of repetitive...
Brower-Toland, Brent, Riddle, Nicole C., Jiang, Hongmei, Huisinga, Kathryn L., Elgin, Sarah C. R.
Methylation of histone H3 lysine 9 (H3K9) is a key feature of silent chromatin and plays an important role in stabilizing the interaction of heterochromatin protein 1 (HP1) with chromatin. Genomes of...
Drosophila RISC Component VIG and Its Homolog Vig2 Impact Heterochromatin Formation
Gracheva, Elena, Dus, Monica, Elgin, Sarah C. R.
Heterochromatin formation plays an important role in gene regulation and the maintenance of genome integrity. Here we present results from a study of the D. melanogaster gene vig, encoding an RNAi...