Sarah M. Assmann

Publication List Details

Period

1993 - 2009

Number

54

Co-Authors

Corresponding Author: (2009)

Sarah M. Assmann, Ph. D, Réka Albert, Ph. D, Sarah M. Assmann

A major aim of systems biology is the study of the inter-relationships found within and between large biological datasets. Here we describe one systems biology method, in which the tools of network...

Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivoprotein coupling (2008)

Gookin, Timothy E, Kim, Junhyong, Assmann, Sarah M

Abstract Background The classic paradigm of heterotrimeric G-protein signaling describes a heptahelical, membrane-spanning G-protein coupled receptor that physically interacts with an intracellular...

Predicting essential components of signal transduction networks: a dynamic model of guard cell abscisic acid signaling (2006)

Li, Song, Assmann, Sarah M., Albert, Reka

Plants both lose water and take in carbon dioxide through microscopic stomatal pores, each of which is regulated by a surrounding pair of guard cells. During drought, the plant hormone abscisic acid...

Predicting Essential Components of Signal Transduction Networks: A Dynamic Model of Guard Cell Abscisic Acid Signaling (2006)

Song Li, Sarah M. Assmann, Réka Albert

Plants both lose water and take in carbon dioxide through microscopic stomatal pores, each of which is regulated by a surrounding pair of guard cells. During drought, the plant hormone abscisic acid...

Ca2+-Permeable, Outwardly-Rectifying K+ Channels in Mesophyll Cells of Arabidopsis taliana (1998)

Romano, Lisa A., Miedema, Hendrik, Assmann, Sarah M.

Arabidopsis thaliana has become a powerful tool in genetics and molecular biology. In order to use Arabidopsis as a model system for electrophysiological studies on plant cells, a detailed...

Identification of mRNAs and proteins in higher plants using probes from the Band 3 anion transporter of mammals (1997)

Rosenberg, Laurie A., Padgett, Pamela E., Assmann, Sarah M., Walling, Linda L., Leonard, Robert T.

Band 3 is a well characterized ion transporter in mammalian cells. To determine if Zea mays expressed Band 3-related polypeptides, polyclonal antibodies to a 13-amino acid sequence at the carboxyl...

Photosynthesis by Guard Cell Chloroplasts of Vicia faba L.: Effects of Factors Associated with Stomatal Movement (1993)

Wu, Weihua, Assmann, Sarah M.

The properties of photosynthetic O2 evolution by mesophyll cell chloroplasts (MCC) and guard cell chloroplasts (GCC) isolated from protoplasts of Vicia faba L. have been studied and effects on O2...

Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity

Jacob, Tobias, Ritchie, Sian, Assmann, Sarah M., Gilroy, Simon

In guard cells, the plant hormone abscisic acid (ABA) inhibits stomatal opening and induces stomatal closure through the coordinated regulation of ion transport. Despite this central role of ABA in...

Ozone inhibits guard cell K+ channels implicated in stomatal opening

Torsethaugen, Gro, Pell, Eva J., Assmann, Sarah M.

Ozone (O3) deleteriously affects organisms ranging from humans to crop plants, yet little is understood regarding the underlying mechanisms. In plants, O3 decreases CO2 assimilation, but whether this...

Differential Responses of Abaxial and Adaxial Guard Cells of Broad Bean to Abscisic Acid and Calcium1

Wang, Xi-Qing, Wu, Wei-Hua, Assmann, Sarah M.

Regulation by abscisic acid (ABA) and Ca2+ of broad bean (Vicia faba) abaxial and adaxial guard cell movements and inward K+ currents were compared. One millimolar Ca2+ in the bathing medium...

Guard Cells Possess a Calcium-Dependent Protein Kinase That Phosphorylates the KAT1 Potassium Channel1

Li, Jiaxu, Julie Lee, Yuh-Ru, Assmann, Sarah M.

Increasing evidence suggests that changes in cytosolic Ca2+ levels and phosphorylation play important roles in the regulation of stomatal aperture and as ion transporters of guard cells. However,...

Distinct Abscisic Acid Signaling Pathways for Modulation of Guard Cell versus Mesophyll Cell Potassium Channels Revealed by Expression Studies in Xenopus laevis Oocytes1

Sutton, Fedora, Paul, Sunil S., Wang, Xi-Qing, Assmann, Sarah M.

Regulation of guard cell ion transport by abscisic acid (ABA) and in particular ABA inhibition of a guard cell inward K+ current (IKin) is well documented. However, little is known concerning ABA...

Apparent Absence of a Redox Requirement for Blue Light Activation of Pump Current in Broad Bean Guard Cells1

Taylor, Alison R., Assmann, Sarah M.

In guard cells, membrane hyperpolarization in response to a blue light (BL) stimulus is achieved by the activation of a plasma membrane H+-ATPase. Using the patch clamp technique on broad bean (Vicia...

The Arabidopsis Putative G Protein–Coupled Receptor GCR1 Interacts with the G Protein α Subunit GPA1 and Regulates Abscisic Acid Signaling

Pandey, Sona, Assmann, Sarah M.

Heterotrimeric G proteins composed of α, β, and γ subunits link ligand perception by G protein–coupled receptors (GPCRs) with downstream effectors, providing a ubiquitous signaling mechanism in...

GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination1[w]

Chen, Jin-Gui, Pandey, Sona, Huang, Jirong, Alonso, José M., Ecker, Joseph R., Assmann, Sarah M., ...

Signal recognition by seven-transmembrane (7TM) cell-surface receptors is typically coupled by heterotrimeric G-proteins to downstream effectors in metazoan, fungal, and amoeboid cells. Some...

Enhancement of the Stomatal Response to Blue Light by Red Light, Reduced Intercellular Concentrations of CO2, and Low Vapor Pressure Differences 1

Assmann, Sarah M.

The effects of environmental parameters on the blue light response of stomata were studied by quantifying transient increases in stomatal conductance in Commelina communis following 15 seconds by...

The Magnitude of the Stomatal Response to Blue Light 1: Modulation by Atmospheric Humidity

Assmann, Sarah M., Grantz, David A.

The effect of leaf-air vapor pressure difference (VPD) on the magnitude of the stomatal response to blue light was investigated in soybean (Glycine max) by administering blue light pulses (22 seconds...

Stomatal Limitation to Carbon Gain in Paphiopedilum sp. (Orchidaceae) and Its Reversal by Blue Light 1

Zeiger, Eduardo, Grivet, C., Assmann, Sarah M., Deitzer, Gerald F., Hannegan, M. W.

Leaves from Paphiopedilum sp. (Orchidaceae) having achlorophyllous stomata, show reduced levels of stomatal conductance when irradiated with red light, as compared with either the related,...

Stomatal Responses to CO2 in Paphiopedilum and Phragmipedium1: Role of the Guard Cell Chloroplast

Assmann, Sarah M., Zeiger, Eduardo

A role of the guard cell chloroplasts in the CO2 response of stomata was investigated through a comparison of the leaf gas exchange characteristics of two closely related orchids: Paphiopedilum...

Arabidopsis Sphingosine Kinase and the Effects of Phytosphingosine-1-Phosphate on Stomatal Aperture1[w]

Coursol, Sylvie, Le Stunff, Hervé, Lynch, Daniel V., Gilroy, Simon, Assmann, Sarah M., Spiegel, Sarah

Sphingolipids are a major component of membrane lipids and their metabolite sphingosine-1-phosphate (S1P) is a potent lipid mediator in animal cells. Recently, we have shown that the enzyme...

Cellular Distribution of Calmodulin and Calmodulin-Binding Proteins in Vicia faba L. 1

Ling, Vincent, Assmann, Sarah M.

The distribution of calmodulin (CaM) and CaM-binding proteins within Vicia faba was investigated. Both CaM and CaM-binding proteins were found to be differentially distributed among organs, tissues,...

Rapid and Specific Modulation of Stomatal Conductance by Blue Light in Ivy (Hedera helix) 12: An Approach to Assess the Stomatal Limitation of Carbon Assimilation

Karlsson, Per Erik, Assmann, Sarah M.

Low intensity (0.015 millimole per square meter per second) blue light applied to leaves of Hedera helix under a high intensity red light background (0.50 millimole per square meter per second red...

Synergistic Effect of Light and Fusicoccin on Stomatal Opening 1: Epidermal Peel and Patch Clamp Experiments

Assmann, Sarah M., Schwartz, Amnon

Upon incubation of epidermal peels of Commelina communis in 1 millimolar KCl, a synergistic effect of light and low fusicoccin (FC) concentrations on stomatal opening is observed. In 1 millimolar...

Plants: the latest model system for G-protein research

Jones, Alan M., Assmann, Sarah M.

In humans, heterotrimeric G proteins couple stimulus perception by G-protein-coupled receptors (GPCRs) with numerous downstream effectors. By contrast, despite great complexity in their...

Predicting Essential Components of Signal Transduction Networks: A Dynamic Model of Guard Cell Abscisic Acid Signaling

Li, Song, Assmann, Sarah M, Albert, Réka

Plants both lose water and take in carbon dioxide through microscopic stomatal pores, each of which is regulated by a surrounding pair of guard cells. During drought, the plant hormone abscisic acid...

Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity

Jacob, Tobias, Ritchie, Sian, Assmann, Sarah M., Gilroy, Simon

In guard cells, the plant hormone abscisic acid (ABA) inhibits stomatal opening and induces stomatal closure through the coordinated regulation of ion transport. Despite this central role of ABA in...

Ozone inhibits guard cell K+ channels implicated in stomatal opening

Torsethaugen, Gro, Pell, Eva J., Assmann, Sarah M.

Ozone (O3) deleteriously affects organisms ranging from humans to crop plants, yet little is understood regarding the underlying mechanisms. In plants, O3 decreases CO2 assimilation, but whether this...

Differential Responses of Abaxial and Adaxial Guard Cells of Broad Bean to Abscisic Acid and Calcium1

Wang, Xi-Qing, Wu, Wei-Hua, Assmann, Sarah M.

Regulation by abscisic acid (ABA) and Ca2+ of broad bean (Vicia faba) abaxial and adaxial guard cell movements and inward K+ currents were compared. One millimolar Ca2+ in the bathing medium...

Guard Cells Possess a Calcium-Dependent Protein Kinase That Phosphorylates the KAT1 Potassium Channel1

Li, Jiaxu, Julie Lee, Yuh-Ru, Assmann, Sarah M.

Increasing evidence suggests that changes in cytosolic Ca2+ levels and phosphorylation play important roles in the regulation of stomatal aperture and as ion transporters of guard cells. However,...

Distinct Abscisic Acid Signaling Pathways for Modulation of Guard Cell versus Mesophyll Cell Potassium Channels Revealed by Expression Studies in Xenopus laevis Oocytes1

Sutton, Fedora, Paul, Sunil S., Wang, Xi-Qing, Assmann, Sarah M.

Regulation of guard cell ion transport by abscisic acid (ABA) and in particular ABA inhibition of a guard cell inward K+ current (IKin) is well documented. However, little is known concerning ABA...

Apparent Absence of a Redox Requirement for Blue Light Activation of Pump Current in Broad Bean Guard Cells1

Taylor, Alison R., Assmann, Sarah M.

In guard cells, membrane hyperpolarization in response to a blue light (BL) stimulus is achieved by the activation of a plasma membrane H+-ATPase. Using the patch clamp technique on broad bean (Vicia...

The Arabidopsis Putative G Protein–Coupled Receptor GCR1 Interacts with the G Protein α Subunit GPA1 and Regulates Abscisic Acid Signaling

Pandey, Sona, Assmann, Sarah M.

Heterotrimeric G proteins composed of α, β, and γ subunits link ligand perception by G protein–coupled receptors (GPCRs) with downstream effectors, providing a ubiquitous signaling mechanism in...

GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination1[w]

Chen, Jin-Gui, Pandey, Sona, Huang, Jirong, Alonso, José M., Ecker, Joseph R., Assmann, Sarah M., ...

Signal recognition by seven-transmembrane (7TM) cell-surface receptors is typically coupled by heterotrimeric G-proteins to downstream effectors in metazoan, fungal, and amoeboid cells. Some...

Enhancement of the Stomatal Response to Blue Light by Red Light, Reduced Intercellular Concentrations of CO2, and Low Vapor Pressure Differences 1

Assmann, Sarah M.

The effects of environmental parameters on the blue light response of stomata were studied by quantifying transient increases in stomatal conductance in Commelina communis following 15 seconds by...

The Magnitude of the Stomatal Response to Blue Light 1: Modulation by Atmospheric Humidity

Assmann, Sarah M., Grantz, David A.

The effect of leaf-air vapor pressure difference (VPD) on the magnitude of the stomatal response to blue light was investigated in soybean (Glycine max) by administering blue light pulses (22 seconds...

Stomatal Limitation to Carbon Gain in Paphiopedilum sp. (Orchidaceae) and Its Reversal by Blue Light 1

Zeiger, Eduardo, Grivet, C., Assmann, Sarah M., Deitzer, Gerald F., Hannegan, M. W.

Leaves from Paphiopedilum sp. (Orchidaceae) having achlorophyllous stomata, show reduced levels of stomatal conductance when irradiated with red light, as compared with either the related,...

Stomatal Responses to CO2 in Paphiopedilum and Phragmipedium1: Role of the Guard Cell Chloroplast

Assmann, Sarah M., Zeiger, Eduardo

A role of the guard cell chloroplasts in the CO2 response of stomata was investigated through a comparison of the leaf gas exchange characteristics of two closely related orchids: Paphiopedilum...

Arabidopsis Sphingosine Kinase and the Effects of Phytosphingosine-1-Phosphate on Stomatal Aperture1[w]

Coursol, Sylvie, Le Stunff, Hervé, Lynch, Daniel V., Gilroy, Simon, Assmann, Sarah M., Spiegel, Sarah

Sphingolipids are a major component of membrane lipids and their metabolite sphingosine-1-phosphate (S1P) is a potent lipid mediator in animal cells. Recently, we have shown that the enzyme...

Cellular Distribution of Calmodulin and Calmodulin-Binding Proteins in Vicia faba L. 1

Ling, Vincent, Assmann, Sarah M.

The distribution of calmodulin (CaM) and CaM-binding proteins within Vicia faba was investigated. Both CaM and CaM-binding proteins were found to be differentially distributed among organs, tissues,...

Rapid and Specific Modulation of Stomatal Conductance by Blue Light in Ivy (Hedera helix) 12: An Approach to Assess the Stomatal Limitation of Carbon Assimilation

Karlsson, Per Erik, Assmann, Sarah M.

Low intensity (0.015 millimole per square meter per second) blue light applied to leaves of Hedera helix under a high intensity red light background (0.50 millimole per square meter per second red...

Synergistic Effect of Light and Fusicoccin on Stomatal Opening 1: Epidermal Peel and Patch Clamp Experiments

Assmann, Sarah M., Schwartz, Amnon

Upon incubation of epidermal peels of Commelina communis in 1 millimolar KCl, a synergistic effect of light and low fusicoccin (FC) concentrations on stomatal opening is observed. In 1 millimolar...

Plants: the latest model system for G-protein research

Jones, Alan M., Assmann, Sarah M.

In humans, heterotrimeric G proteins couple stimulus perception by G-protein-coupled receptors (GPCRs) with numerous downstream effectors. By contrast, despite great complexity in their...

Predicting Essential Components of Signal Transduction Networks: A Dynamic Model of Guard Cell Abscisic Acid Signaling

Li, Song, Assmann, Sarah M, Albert, Réka

Plants both lose water and take in carbon dioxide through microscopic stomatal pores, each of which is regulated by a surrounding pair of guard cells. During drought, the plant hormone abscisic acid...

G-Protein Complex Mutants Are Hypersensitive to Abscisic Acid Regulation of Germination and Postgermination Development1[W]

Pandey, Sona, Chen, Jin-Gui, Jones, Alan M., Assmann, Sarah M.

Abscisic acid (ABA) plays regulatory roles in a host of physiological processes throughout plant growth and development. Seed germination, early seedling development, stomatal guard cell functions,...

Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling

Gookin, Timothy E, Kim, Junhyong, Assmann, Sarah M

Computational prediction and in vivo protein coupling experiments identify candidate plant G-protein coupled receptors in Arabidopsis, rice and poplar.

Abscisic acid regulation of guard-cell K+ and anion channels in Gβ- and RGS-deficient Arabidopsis lines

Fan, Liu-Min, Zhang, Wei, Chen, Jin-Gui, Taylor, J. Philip, Jones, Alan M., Assmann, Sarah M.

In mammals, basal currents through G protein-coupled inwardly rectifying K+ (GIRK) channels are repressed by Gαi/oGDP, and the channels are activated by direct binding of free Gβγ subunits...

Gγ1 + Gγ2 ≠ Gβ: Heterotrimeric G Protein Gγ-Deficient Mutants Do Not Recapitulate All Phenotypes of Gβ-Deficient Mutants1[C][W][OA]

Trusov, Yuri, Zhang, Wei, Assmann, Sarah M., Botella, José Ramón

Heterotrimeric G proteins are signaling molecules ubiquitous among all eukaryotes. The Arabidopsis (Arabidopsis thaliana) genome contains one Gα (GPA1), one Gβ (AGB1), and two Gγ subunit (AGG1 and...

Functional Proteomics of Arabidopsis thaliana Guard Cells Uncovers New Stomatal Signaling Pathways[W][OA]

Zhao, Zhixin, Zhang, Wei, Stanley, Bruce A., Assmann, Sarah M.

We isolated a total of 3 × 108 guard cell protoplasts from 22,000 Arabidopsis thaliana plants and identified 1734 unique proteins using three complementary proteomic methods: protein spot...