Characterization of a mitochondrial iron regulated gene (MIR) in rice (2009)
Bashir, Khurram, Ishimaru, Yasuhiro, Fujimoto, Masaru, An, Gynheung, Itai, Reiko N, Tsutsumi, Nobuhiro, ...
Mitochondria utilize iron (Fe), but the proteins involved in mitochondrial Fe regulation have not been characterized in plants. We cloned and characterized a mitochondrial iron-regulated (MIR) gene...
Characterization of a rice Mitochondrial Iron Transporter (OsMIT) (2009)
Bashir, Khurram, Ishimaru, Yasuhiro, Fujimoto, Masaru, An, Gynheung, Tsutsumi, Nobuhiro, Kaplan, Jerry, ...
Mitochondrial iron (Fe) homeostasis is important for various cellular processes including heme synthesis and Fe-S cluster synthesis, however the proteins involved in mitochondrial Fe uptake and...
The novel transcription factor IDEF1 regulates iron-deficiency response and tolerance (2009)
Kobayashi, Takanori, Ogo, Yuko, Nakanishi Itai, Reiko, Nakanishi, Hiromi, Takahashi, Michiko, Mori, Satoshi, ...
Iron is essential for most living organisms and is required for normal plant growth. Plants induce iron utilization systems under conditions of low iron availability, but the molecular mechanisms of...
Kakei, Yusuke, Yamaguchi, Isomaro, Takahashi, Michiko, Nakanishi, Hiromi, Mori, Satoshi, Nishizawa, Naoko K
A highly sensitive quantitative method for assaying nicotianamine (NA) and 2'-deoxymugineic acid (DMA) using liquid chromatography electrospray ionization time-of-flight mass spectrometry...
Ogo, Yuko, Kobayashi, Takanori, Itai, Reiko Nakanishi, Nakanishi, Hiromi, Kakei, Yusuke, Takahashi, Michiko, ...
Iron (Fe) is essential for most living organisms, and thus Fe deficiency poses a major abiotic stress in crop production. Plants induce Fe utilization systems under conditions of low-Fe availability,...
Overexpression of OsIRO2 improves both iron uptake and translocation to seeds in rice (2009)
Ogo, Yuko, Itai, Reiko Nakanishi, Kobayashi, Takanori, Nakanishi, Hiromi, Mori, Satoshi, Nishizawa, Naoko K
Iron deficiency is a worldwide agricultural problem on calcareous soils with low iron (Fe) availability and also poses a major human nutritional problem. Plants induce Fe-acquisition systems under...
Tsukamoto, Takashi, Nakanishi, Hiromi, Uchida, Hiroshi, Watanabe, Satoshi, Matsuhashi, Shinpei, Mori, Satoshi, ...
The real-time translocation of iron (Fe) in barley (Hordeum vulgare L. cv. Ehimehadaka no. 1) was visualized using the positron-emitting tracer 52Fe and a positron-emitting tracer imaging system...
Ishimaru, Yasuhiro, Masuda, Hiroshi, Suzuki, Motofumi, Bashir, Khurram, Takahashi, Michiko, Nakanishi, Hiromi, ...
Zinc (Zn), an essential nutrient in cells, plays a vital role in controlling cellular processes such as growth, development, and differentiation. Although the mechanisms of Zn translocation in rice...
Ogo, Yuko, Itai, Reiko Nakanishi, Nakanishi, Hiromi, Inoue, Haruhiko, Kobayashi, Takanori, Suzuki, Motofumi, ...
To clarify the molecular mechanism that regulates iron (Fe) acquisition in graminaceous plants, a time-course analysis of gene expression during Fe deficiency stress was conducted using a rice 22K...
Ogo, Yuko, Itai, Reiko Nakanishi, Nakanishi, Hiromi, Inoue, Haruhiko, Kobayashi, Takanori, Suzuki, Motofumi, ...
To clarify the molecular mechanism that regulates iron (Fe) acquisition in graminaceous plants, a time-course analysis of gene expression during Fe deficiency stress was conducted using a rice 22K...
Kobayashi, Takanori, Suzuki, Motofumi, Inoue, Haruhiko, Itai, Reiko Nakanishi, Takahashi, Michiko, Nakanishi, Hiromi, ...
Rice plants (Oryza sativa L.) utilize the iron chelators known as mugineic acid family phytosiderophores (MAs) to acquire iron from the rhizosphere. Synthesis of MAs and uptake of MA-chelated iron...
OsZIP4, a novel zinc-regulated zinc transporter in rice (2005)
Ishimaru, Yasuhiro, Suzuki, Motofumi, Kobayashi, Takanori, Takahashi, Michiko, Nakanishi, Hiromi, Mori, Satoshi, ...
Zinc (Zn) is an essential element for the normal growth of plants but information is scarce on the mechanisms whereby Zn is transported in rice (Oryza sativa L.) plants. Four distinct genes, OsZIP4,...
Kim, Suyeon, Takahashi, Michiko, Higuchi, Kyoko, Tsunoda, Kyoko, Nakanishi, Hiromi, Yoshimura, Etsuro, ...
Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco...
OsZIP4, a novel zinc-regulated zinc transporter in rice (2005)
Ishimaru, Yasuhiro, Suzuki, Motofumi, Kobayashi, Takanori, Takahashi, Michiko, Nakanishi, Hiromi, Mori, Satoshi, ...
Zinc (Zn) is an essential element for the normal growth of plants but information is scarce on the mechanisms whereby Zn is transported in rice (Oryza sativa L.) plants. Four distinct genes, OsZIP4,...
Kim, Suyeon, Takahashi, Michiko, Higuchi, Kyoko, Tsunoda, Kyoko, Nakanishi, Hiromi, Yoshimura, Etsuro, ...
Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco...
Gene manipulation of fluorescent protein through site-selective hydrolysis by Ce(IV)/EDTA (2005)
Kitamura, Yoshihito, Mori, Satoshi, Komiyama, Makoto
Previously we reported that gap-site, formed in substrate DNA by using two oligonucleotide additives, was selectively hydrolyzed by Ce(IV)/EDTA. Herein this site-selective scission was used for...
Kobayashi, Takanori, Suzuki, Motofumi, Inoue, Haruhiko, Itai, Reiko Nakanishi, Takahashi, Michiko, Nakanishi, Hiromi, ...
Rice plants (Oryza sativa L.) utilize the iron chelators known as mugineic acid family phytosiderophores (MAs) to acquire iron from the rhizosphere. Synthesis of MAs and uptake of MA-chelated iron...
Kobayashi, Takanori, Suzuki, Motofumi, Inoue, Haruhiko, Itai, Reiko Nakanishi, Takahashi, Michiko, Nakanishi, Hiromi, ...
Rice plants (Oryza sativa L.) utilize the iron chelators known as mugineic acid family phytosiderophores (MAs) to acquire iron from the rhizosphere. Synthesis of MAs and uptake of MA-chelated iron...
OsZIP4, a novel zinc-regulated zinc transporter in rice (2005)
Ishimaru, Yasuhiro, Suzuki, Motofumi, Kobayashi, Takanori, Takahashi, Michiko, Nakanishi, Hiromi, Mori, Satoshi, ...
Zinc (Zn) is an essential element for the normal growth of plants but information is scarce on the mechanisms whereby Zn is transported in rice (Oryza sativa L.) plants. Four distinct genes, OsZIP4,...
Kim, Suyeon, Takahashi, Michiko, Higuchi, Kyoko, Tsunoda, Kyoko, Nakanishi, Hiromi, Yoshimura, Etsuro, ...
Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco...
Effect of c-fos overexpression on development and proliferation of peritoneal B cells (2004)
Mori, Satoshi, Sakamoto, Akemi, Yamashita, Kimihiro, Fujimura, Lisa, Arima, Masafumi, Hatano, Masahiko, ...
We examined effects of c-fos overexpression on the development and property of peritoneal B-1 cells using transgenic (H2-c-fos) mice carrying the c-fos gene under the control of the constitutive...
Cloning an iron-regulated metal transporter from rice (2002)
Bughio, Naimatullah, Yamaguchi, Hirotaka, Nishizawa, Naoko K., Nakanishi, Hiromi, Mori, Satoshi
Rice cDNA and genomic libraries were screened in order to clone an Fe(II) transporter gene. A cDNA clone highly homologous to the Arabidopsis Fe(II) transporter gene IRT1 was isolated from...
IDI7, a new iron-regulated ABC transporter from barley roots, localizes to the tonoplast (2002)
Yamaguchi, Hirotaka, Nishizawa, Naoko-Kishi, Nakanishi, Hiromi, Mori, Satoshi
A new Fe‐deficiency‐induced cDNA, IDI7, was isolated from the roots of Fe‐deficient barley (Hordeum vulgare L. cv. Ehimehadaka no. 1). The transcript levels of IDI7 in...
Itai, Reiko, Suzuki, Kazuya, Yamaguchi, Hirotaka, Nakanishi, Hiromi, Nishizawa, Naoko-Kishi, Yoshimura, Etsuro, ...
To isolate the genes involved in the response of graminaceous plants to Fe‐deficient stress, a protein induced by Fe‐deficiency treatment was isolated from barley (Hordeum vulgare...
Yamaguchi, Hirotaka, Nakanishi, Hiromi, Nishizawa, Naoko K., Mori, Satoshi
A new Fe‐deficiency‐inducible cDNA, IDI2, was isolated from Fe‐deficient barley roots using the cDNA MACRO Array Technique. Accumulation of IDI2 transcripts in barley roots was...
Light-Dependent Iron Transport into Isolated Barley Chloroplasts (1997)
Bughio, Naimatullah, Takahashi, Michiko, Yoshimura, Esturo, Nishizawa, Naoko-Kishi, Mori, Satoshi
Translocation studies of 59Fe(III)-epihydroxymugineic acid in intact barley plants revealed that Fe transport from leaf veins to mesophyll cells is light-regulated. Similarly, Fe absorption studies...
Higuchi, Kiyoko, Nishizawa, Naoko-Kishi, Yamaguchi, Hirotaka, Römheld, Volker, Marschner, Horst, Mori, Satoshi
In vitro nicotianamine synthase activity was measured in tobacco under Fe-deficient or Fe-sufficient conditions. Its activity was not induced by Fe-deficiency, in contrast to barley roots, implying...
Detection and Characterization of Protein Kinases in Rice Phloem Sap (1995)
Nakamura, Shin-ichi, Hayashi, Hiroaki, Mori, Satoshi, Chino, Mitsuo
Calcium-dependent protein kinases were detected and characterized in the phloem sap of rice plants. Protein phosphorylation was activated in the presence of micromolar levels of free Ca2+ ions but...
Ultrastructural Localization of the Basic 7S Globulin in Soybean (Glycine max) Cotyledons (1994)
Nishizawa, Naoko K., Mori, Satoshi, Watanabe, Yoshihiro, Hirano, Hisashi
The basic 7S globulin (Bg) is a cysteine-rich glycoprotein in soybean seeds. Mature Bg is composed of subunits of high and low molecular mass that are linked by disulfide bonds. Bg and Bg-like...
Kanazawa, Kenji, Higuchi, Kyoko, Nishizawa, Naoko-Kishi, Fushiya, Shinji, Chino, Mitsuo, Mori, Satoshi
The activities of nicotianamine aminotransferase, one of the enzymes for the biosynthesis of mugineic acid-family phytosiderophores, were examined in six graminaceous species. The enzyme activities...
Tomoaki Yoshikawa, Satoshi Mori, A. J. Santiesteban, T. C. Sun, Else Hafstad, Jie Chen, ...
There is anecdotal evidence that bone strains may increase to the point that bone becomes susceptible to rapid failure when muscles become fatigued. To determine whether neuromuscular response could...
Expression of a Gene Specific for Iron Deficiency (Ids3) in the Roots of Hordeum vulgare (1993)
Nakanishi, Hiromi, Okumura, Nami, Umehara, Yosuke, Nishizawa, Naoko-Kishi, Chino, Mitsuo, Mori, Satoshi
To clone genes required for the synthesis of mugineic acid (MA) or for the transport of Fe(III)-MA, a λZAPII cDNA library was constructed from poly(A)+-RNA isolated from Fe-deficient barley roots....
Excess Copper Induces A Cytosolic Cu,Zn-Superoxide Dismutase in Soybean Root (1992)
Chongpraditnun, Praphasri, Mori, Satoshi, Chino, Mitsuo
The induction of several proteins in soybean roots in response to copper was investigated. The major Cu2+-induced protein of 16 kDa was purified by SDS-PAGE and 2D-PAGE. This Cu2+-induced protein...
Development and Characterization of a Monoclonal Antibody to Phytosiderophores (1992)
Mihashi, Shuichi, Sikora, Lawrence J., Buyer, Jeffrey S., Fushiya, Shinji, Mori, Satoshi
Mugineic acid-family phytosiderophores (MAs) are low molecular weight chelators that are secreted by graminaceous plants, form complexes with soil Fe(III) and are essential for plant growth. Methods...
Establishment of a Cell-Free System for the Biosynthesis of Nicotianamine (1989)
Shojima, Shinsuke, Nishizawa, Naoko-Kishi, Mori, Satoshi
A cell-free system has been reported for the biosynthesis of nicotianamine, a putative precursor of mugineic acid and its derivatives (MAs). L-[l-14C]methionine was incubated with a crude homogenate...
Nishizawa, Naoko, Mori, Satoshi
The ultrastructure of the chloroplast in tomato leaves was studied by a liquid helium rapid-freezing and substitution-fixation method. Distinct morphological differences were observed between the...
Methionine as a Dominant Precursor of Phytosiderophores in Graminaceae Plants (1987)
Mori, Satoshi, Nishizawa, Naoko
Nine 14C-labeled amino acids and 14C-acetic acid from root tips of Fe-deficient Graminaceae plants (Hordeum vulgare, Oryzae sativa and Avena saliva) were surveyed to determine the precursor amino...
Desiccation-Induced Heterophagy in Corn Root Cells (1984)
NISHIZAWA, Naoko, MORI, Satoshi
Ultrastructural changes induced by water deficiency were investigated in corn root cells. Many vesicles containing fibrous materials appeared in the cortex cells of desiccated corn roots and not in...
To monitor rapidly the specific activity of radioactive organic acids, a high performance liquid chromatography (HPLC)-radioanalyzer was constructed. The instrument is a linear combination of an HPLC...
A Vesicle Containing Fibrous Materials Appearing in Water-Stressed Corn Root Cells (1981)
Nishizawa, Naoko, Mori, Satoshi
Ultrastructural changes induced by water stress were investigated in corn root cells. Desiccation was imposed by withholding water from the soil. Vesicles containing cellwall-like fibrous materials...
Nishizawa, Naoko, Mori, Satoshi
To demonstrate the utilization of exogenous protein by plant cells, we examined the incorporation of 3H-labeled hemoglobin into rice root cells by electronmicroscope-autoradiography. We showed that...
Endocytosis (heterophagy) in plant cells: Involvement of ER and ER-derived vesicles (1978)
Nishizawa, Naoko, Mori, Satoshi
The ultrastructure of rice roots water-cultured with hemoglobin was investigated by electron microscopy. In the cortex cells of these roots, plasmalemma invaginations were found to be surrounded by...
Invagination of plasmalemma: Its role in the absorption of macromolecules in rice roots (1977)
Nishizawa, Naoko, Mori, Satoshi
In order to investigate the mechanism of entry of organic nitrogen into root cells, the ultrastructure of rice roots water-cultured in medium containing hemoglobin as the sole nitrogen source was...
Higuchi, Kyoko, Suzuki, Kazuya, Nakanishi, Hiromi, Yamaguchi, Hirotaka, Nishizawa, Naoko-Kishi, Mori, Satoshi
Nicotianamine synthase (NAS), the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores, catalyzes the trimerization of S-adenosylmethionine to form one molecule of...
Suzuki, Kazuya, Itai, Reiko, Suzuki, Koichiro, Nakanishi, Hiromi, Nishizawa, Naoko-Kishi, Yoshimura, Etsuro, ...
To identify the proteins induced by Fe deficiency, we have compared the proteins of Fe-sufficient and Fe-deficient barley (Hordeum vulgare L.) roots by two-dimensional polyacrylamide gel...
Takahashi, Michiko, Yamaguchi, Hirotaka, Nakanishi, Hiromi, Shioiri, Takayuki, Nishizawa, Naoko-Kishi, Mori, Satoshi
Nicotianamine aminotransferase (NAAT), the key enzyme involved in the biosynthesis of mugineic acid family phytosiderophores (MAs), catalyzes the amino transfer of nicotianamine (NA). MAs are found...
Kiyomiya, Shoichiro, Nakanishi, Hiromi, Uchida, Hiroshi, Tsuji, Atsunori, Nishiyama, Shingo, Futatsubashi, Masami, ...
The ammonium ion is an indispensable nitrogen source for crops, especially paddy rice (Oryza sativa L. cv Nipponbare). Until now, it has been impossible to measure ammonium uptake and nitrogen...
Role of Nicotianamine in the Intracellular Delivery of Metals and Plant Reproductive Development
Takahashi, Michiko, Terada, Yasuko, Nakai, Izumi, Nakanishi, Hiromi, Yoshimura, Etsuro, Mori, Satoshi, ...
Nicotianamine (NA), a chelator of metals, is ubiquitously present in higher plants. Nicotianamine aminotransferase (NAAT) catalyzes the amino group transfer of NA in the biosynthetic pathway of...
Mizuno, Daichi, Higuchi, Kyoko, Sakamoto, Tatsuya, Nakanishi, Hiromi, Mori, Satoshi, Nishizawa, Naoko K.
Nicotianamine synthase (NAS) is an enzyme that is critical for the biosynthesis of the mugineic acid family of phytosiderophores in graminaceous plants, and for the homeostasis of metal ions in...
Shojima, Shinsuke, Nishizawa, Naoko-Kishi, Fushiya, Shinji, Nozoe, Shigeo, Irifune, Tomohiro, Mori, Satoshi
2′ -Deoxymugineic acid (DMA), one of mugineic acid-family phytosiderophores (MAs), was synthesized in vitro both from l-methionine and from nicotianamine (NA) with a cell-free system derived from...
Higuchi, Kyoko, Suzuki, Kazuya, Nakanishi, Hiromi, Yamaguchi, Hirotaka, Nishizawa, Naoko-Kishi, Mori, Satoshi
Nicotianamine synthase (NAS), the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores, catalyzes the trimerization of S-adenosylmethionine to form one molecule of...
Suzuki, Kazuya, Itai, Reiko, Suzuki, Koichiro, Nakanishi, Hiromi, Nishizawa, Naoko-Kishi, Yoshimura, Etsuro, ...
To identify the proteins induced by Fe deficiency, we have compared the proteins of Fe-sufficient and Fe-deficient barley (Hordeum vulgare L.) roots by two-dimensional polyacrylamide gel...
Takahashi, Michiko, Yamaguchi, Hirotaka, Nakanishi, Hiromi, Shioiri, Takayuki, Nishizawa, Naoko-Kishi, Mori, Satoshi
Nicotianamine aminotransferase (NAAT), the key enzyme involved in the biosynthesis of mugineic acid family phytosiderophores (MAs), catalyzes the amino transfer of nicotianamine (NA). MAs are found...
Kiyomiya, Shoichiro, Nakanishi, Hiromi, Uchida, Hiroshi, Tsuji, Atsunori, Nishiyama, Shingo, Futatsubashi, Masami, ...
The ammonium ion is an indispensable nitrogen source for crops, especially paddy rice (Oryza sativa L. cv Nipponbare). Until now, it has been impossible to measure ammonium uptake and nitrogen...
Role of Nicotianamine in the Intracellular Delivery of Metals and Plant Reproductive Development
Takahashi, Michiko, Terada, Yasuko, Nakai, Izumi, Nakanishi, Hiromi, Yoshimura, Etsuro, Mori, Satoshi, ...
Nicotianamine (NA), a chelator of metals, is ubiquitously present in higher plants. Nicotianamine aminotransferase (NAAT) catalyzes the amino group transfer of NA in the biosynthetic pathway of...
Mizuno, Daichi, Higuchi, Kyoko, Sakamoto, Tatsuya, Nakanishi, Hiromi, Mori, Satoshi, Nishizawa, Naoko K.
Nicotianamine synthase (NAS) is an enzyme that is critical for the biosynthesis of the mugineic acid family of phytosiderophores in graminaceous plants, and for the homeostasis of metal ions in...
Shojima, Shinsuke, Nishizawa, Naoko-Kishi, Fushiya, Shinji, Nozoe, Shigeo, Irifune, Tomohiro, Mori, Satoshi
2′ -Deoxymugineic acid (DMA), one of mugineic acid-family phytosiderophores (MAs), was synthesized in vitro both from l-methionine and from nicotianamine (NA) with a cell-free system derived from...
Ishimaru, Yasuhiro, Kim, Suyeon, Tsukamoto, Takashi, Oki, Hiroyuki, Kobayashi, Takanori, Watanabe, Satoshi, ...
Iron (Fe) deficiency is a worldwide agricultural problem on calcareous soils with low-Fe availability due to high soil pH. Rice plants use a well documented phytosiderophore-based system (Strategy...
Deoxymugineic acid increases Zn translocation in Zn-deficient rice plants
Suzuki, Motofumi, Tsukamoto, Takashi, Inoue, Haruhiko, Watanabe, Satoshi, Matsuhashi, Shinpei, Takahashi, Michiko, ...
Deoxymugineic acid (DMA) is a member of the mugineic acid family phytosiderophores (MAs), which are natural metal chelators produced by graminaceous plants. Rice secretes DMA in response to Fe...
The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants
Kobayashi, Takanori, Ogo, Yuko, Itai, Reiko Nakanishi, Nakanishi, Hiromi, Takahashi, Michiko, Mori, Satoshi, ...
Iron is essential for most living organisms and is often the major limiting nutrient for normal growth. Plants induce iron utilization systems under conditions of low iron availability, but the...
Tsukamoto, Takashi, Nakanishi, Hiromi, Uchida, Hiroshi, Watanabe, Satoshi, Matsuhashi, Shinpei, Mori, Satoshi, ...
The real-time translocation of iron (Fe) in barley (Hordeum vulgare L. cv. Ehimehadaka no. 1) was visualized using the positron-emitting tracer 52Fe and a positron-emitting tracer imaging system...
Aoyama, Takahiro, Kobayashi, Takanori, Takahashi, Michiko, Nagasaka, Seiji, Usuda, Kanako, Kakei, Yusuke, ...
Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the...