Sayyed K. Zaidi

Altered Runx1 subnuclear targeting enhances myeloid cell proliferation and blocks differentiation by activating a miR-24/MKP-7/MAPK network (2009)

Zaidi, Sayyed K., Dowdy, Christopher R., Van Wijnen, Andre J., Lian, Jane B., Raza, Azra, Stein, Janet L., ...

Disruption of Runx1/AML1 subnuclear localization, either by a single amino acid substitution or by a chromosomal translocation [e.g., t(8;21)], is linked to the etiology of acute myeloid leukemia...

Organization, integration, and assembly of genetic and epigenetic regulatory machinery in nuclear microenvironments: implications for biological control in cancer (2009)

Stein, Gary S., Zaidi, Sayyed K., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., ...

There is growing awareness that the fidelity of gene expression necessitates coordination of transcription factor metabolism and organization of genes and regulatory proteins within the...

Transcription-factor-mediated epigenetic control of cell fate and lineage commitment (2009)

Stein, Gary S., Zaidi, Sayyed K., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., ...

Epigenetic control is required to maintain competency for the activation and suppression of genes during cell division. The association between regulatory proteins and target gene loci during mitosis...

Runx2 induces acute myeloid leukemia in cooperation with Cbfbeta-SMMHC in mice (2009)

Kuo, Ya-Huei, Zaidi, Sayyed K., Gornostaeva, Svetlana, Komori, Toshihisa, Stein, Gary S., Castilla, Lucio H.

The core-binding factor (CBF) is a master regulator of developmental and differentiation programs, and CBF alterations are frequently associated with acute leukemia. The role of the CBF member RUNX2...

Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes (2008)

Pande, Sandhya, Ali, Syed A., Dowdy, Christopher, Zaidi, Sayyed K., Ito, Kosei, Ito, Yoshiaki, ...

Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation...

Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes (2008)

Pande, Sandhya, Ali, Syed A., Dowdy, Christopher, Zaidi, Sayyed K., Ito, Kosei, Ito, Yoshiaki, ...

Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation...

The leukemogenic t(8;21) fusion protein AML1-ETO controls rRNA genes and associates with nucleolar-organizing regions at mitotic chromosomes (2008)

Bakshi, Rachit, Zaidi, Sayyed K., Pande, Sandhya, Hassan, Mohammad Q., Young, Daniel W., Montecino, Martin A., ...

RUNX1/AML1 is required for definitive hematopoiesis and is frequently targeted by chromosomal translocations in acute myeloid leukemia (AML). The t(8;21)-related AML1-ETO fusion protein blocks...

The leukemogenic t(8;21) fusion protein AML1-ETO controls rRNA genes and associates with nucleolar-organizing regions at mitotic chromosomes (2008)

Bakshi, Rachit, Zaidi, Sayyed K., Pande, Sandhya, Hassan, Mohammad Q., Young, Daniel W., Montecino, Martin A., ...

RUNX1/AML1 is required for definitive hematopoiesis and is frequently targeted by chromosomal translocations in acute myeloid leukemia (AML). The t(8;21)-related AML1-ETO fusion protein blocks...

The histone gene cell cycle regulator HiNF-P is a unique zinc finger transcription factor with a novel conserved auxiliary DNA-binding motif (2008)

Medina, Ricardo F., Buck, Timothy F., Zaidi, Sayyed K., Miele, Angela, Lian, Jane B., Stein, Janet L., ...

Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of...

The histone gene cell cycle regulator HiNF-P is a unique zinc finger transcription factor with a novel conserved auxiliary DNA-binding motif (2008)

Medina, Ricardo F., Buck, Timothy F., Zaidi, Sayyed K., Miele, Angela, Lian, Jane B., Stein, Janet L., ...

Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of...

Runx2 transcriptional activation of Indian Hedgehog and a downstream bone metastatic pathway in breast cancer cells (2008)

Pratap, Jitesh, Wixted, John J., Gaur, Tripti, Zaidi, Sayyed K., Dobson, Jason, Gokul, Karthiga Devi, ...

Runx2, required for bone formation, is ectopically expressed in breast cancer cells. To address the mechanism by which Runx2 contributes to the osteolytic disease induced by MDA-MB-231 cells, we...

Runx2 transcriptional activation of Indian Hedgehog and a downstream bone metastatic pathway in breast cancer cells (2008)

Pratap, Jitesh, Wixted, John J., Gaur, Tripti, Zaidi, Sayyed K., Dobson, Jason, Gokul, Karthiga Devi, ...

Runx2, required for bone formation, is ectopically expressed in breast cancer cells. To address the mechanism by which Runx2 contributes to the osteolytic disease induced by MDA-MB-231 cells, we...

Nuclear microenvironments and cancer (2008)

Stein, Gary S., Davie, James R., Knowlton, J. Randy, Zaidi, Sayyed K.

Nucleic acids and regulatory proteins are architecturally organized in nuclear microenvironments. The compartmentalization of regulatory machinery for gene expression, replication and repair, is...

Genetic and epigenetic regulation in nuclear microenvironments for biological control in cancer (2008)

Stein, Gary S., Zaidi, Sayyed K., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., ...

The regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex...

Chromatin immunoprecipitation assays: application of ChIP-on-chip for defining dynamic transcriptional mechanisms in bone cells (2008)

Van Der Deen, Margaretha, Hassan, Mohammad Q., Pratap, Jitesh, Teplyuk, Nadiya M., Young, Daniel W., Javed, Amjad, ...

Normal cell growth and differentiation of bone cells requires the sequential expression of cell type specific genes to permit lineage specification and development of cellular phenotypes....

In situ nuclear organization of regulatory machinery (2008)

Pockwinse, Shirwin M., Zaidi, Sayyed K., Medina, Ricardo F., Bakshi, Rachit, Kota, Krishna P., Ali, Syed A., ...

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of...

Phenotypic transcription factors epigenetically mediate cell growth control (2008)

Ali, Syed A., Zaidi, Sayyed K., Dacwag, Caroline S., Salma, Nunciada, Young, Daniel W., Shakoori, A. Rauf, ...

Ribosomal RNA (rRNA) genes are down-regulated during osteogenesis, myogenesis, and adipogenesis, necessitating a mechanistic understanding of interrelationships between growth control and phenotype...

Phenotypic transcription factors epigenetically mediate cell growth control (2008)

Ali, Syed A., Zaidi, Sayyed K., Dacwag, Caroline S., Salma, Nunciada, Young, Daniel W., Shakoori, A. Rauf, ...

Ribosomal RNA (rRNA) genes are down-regulated during osteogenesis, myogenesis, and adipogenesis, necessitating a mechanistic understanding of interrelationships between growth control and phenotype...

MicroRNAs 221 and 222 bypass quiescence and compromise cell survival (2008)

Medina, Ricardo F., Zaidi, Sayyed K., Liu, Chang-Gong, Stein, Janet L., Van Wijnen, Andre J., Croce, Carlo M., ...

MicroRNAs (miRNA) have tumor suppressive and oncogenic potential in human cancer, but whether and how miRNAs control cell cycle progression is not understood. To address this question, we carried out...

MicroRNAs 221 and 222 bypass quiescence and compromise cell survival (2008)

Medina, Ricardo F., Zaidi, Sayyed K., Liu, Chang-Gong, Stein, Janet L., Van Wijnen, Andre J., Croce, Carlo M., ...

MicroRNAs (miRNA) have tumor suppressive and oncogenic potential in human cancer, but whether and how miRNAs control cell cycle progression is not understood. To address this question, we carried out...

Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal (2008)

Javed, Amjad, Bae, Jong-Sup, Afzal, Faiza, Gutierrez, Soraya E., Pratap, Jitesh, Zaidi, Sayyed K., ...

Two regulatory pathways, bone morphogenetic protein (BMP)/transforming growth factor-beta (TGFbeta) and the transcription factor RUNX2, are required for bone formation in vivo. Here we show the...

Runx2 deficiency and defective subnuclear targeting bypass senescence to promote immortalization and tumorigenic potential (2007)

Zaidi, Sayyed K., Pande, Sandhya, Pratap, Jitesh, Gaur, Tripti, Grigoriu, Simina R., Ali, Syed A., ...

The osteogenic Runt-related (Runx2) transcription factor negatively regulates proliferation and ribosomal gene expression in normal diploid osteoblasts, but is up-regulated in metastatic breast and...

Runx2 deficiency and defective subnuclear targeting bypass senescence to promote immortalization and tumorigenic potential (2007)

Zaidi, Sayyed K., Pande, Sandhya, Pratap, Jitesh, Gaur, Tripti, Grigoriu, Simina R., Ali, Syed A., ...

The osteogenic Runt-related (Runx2) transcription factor negatively regulates proliferation and ribosomal gene expression in normal diploid osteoblasts, but is up-regulated in metastatic breast and...

Nuclear microenvironments in biological control and cancer (2007)

Zaidi, Sayyed K., Young, Daniel W., Javed, Amjad, Pratap, Jitesh, Montecino, Martin A., Van Wijnen, Andre J., ...

Nucleic acids and regulatory proteins are compartmentalized in microenvironments within the nucleus. This subnuclear organization may support convergence and the integration of physiological signals...

Organization of transcriptional regulatory machinery in nuclear microenvironments: implications for biological control and cancer (2007)

Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Javed, Amjad, Montecino, Martin A., ...

Mitotic retention of gene expression patterns by the cell fate-determining transcription factor Runx2 (2007)

Young, Daniel W., Hassan, Mohammad Q., Yang, Xiaoqing, Galindo, Mario, Javed, Amjad, Zaidi, Sayyed K., ...

During cell division, cessation of transcription is coupled with mitotic chromosome condensation. A fundamental biological question is how gene expression patterns are retained during mitosis to...

Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2 (2007)

Young, Daniel W., Hassan, Mohammad Q., Pratap, Jitesh, Galindo, Mario, Zaidi, Sayyed K., Lee, Suk Hee, ...

Regulation of ribosomal RNA genes is a fundamental process that supports the growth of cells and is tightly coupled with cell differentiation. Although rRNA transcriptional control by RNA polymerase...

An architectural perspective of cell-cycle control at the G1/S phase cell-cycle transition (2006)

Stein, Gary S., Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., Montecino, Martin A., Zaidi, Sayyed K., ...

A prominent role for the execution of cell cycle and growth regulatory mechanisms within the three-dimensional context of nuclear architecture is becoming increasingly evident. Signaling pathways and...

Alterations in intranuclear localization of Runx2 affect biological activity (2006)

Zaidi, Sayyed K., Javed, Amjad, Pratap, Jitesh, Schroeder, Tania M., Westendorf, Jennifer J., Lian, Jane B., ...

The transcription factor Runx2 controls osteoblast proliferation and differentiation. Runx2 organizes and assembles gene-regulatory complexes in nuclear microenvironments where target genes are...

Combinatorial organization of the transcriptional regulatory machinery in biological control and cancer (2005)

Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Javed, Amjad, Montecino, Martin A., ...

The architecturally associated subnuclear organization of nucleic acids and cognate regulatory factors suggests functional interrelationships between nuclear structure and gene expression. Mechanisms...

HiNF-P directly links the cyclin E/CDK2/p220NPAT pathway to histone H4 gene regulation at the G1/S phase cell cycle transition (2005)

Miele, Angela, Braastad, Corey D., Holmes, William F., Mitra, Partha, Medina, Ricardo F., Xie, Ronglin, ...

Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the...

Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype (2005)

Vradii, Diana, Zaidi, Sayyed K., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.

The multifunctional C terminus of the hematopoietic AML1 transcription factor interacts with coregulatory proteins, supports the convergence and integration of physiological signals, and contains the...

The dynamic organization of gene-regulatory machinery in nuclear microenvironments (2005)

Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin A., ...

Nuclear components are functionally linked with the dynamic temporal and spatial compartmentalization, sorting and integration of regulatory information to facilitate its selective use. For example,...

Quantitative signature for architectural organization of regulatory factors using intranuclear informatics (2004)

Young, Daniel W., Zaidi, Sayyed K., Furcinitti, Paul S., Javed, Amjad, Van Wijnen, Andre J., Stein, Janet L., ...

Regulatory machinery for replication and gene expression is punctately organized in supramolecular complexes that are compartmentalized in nuclear microenvironments. Quantitative approaches are...

Intranuclear trafficking: organization and assembly of regulatory machinery for combinatorial biological control (2004)

Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin A., ...

The molecular logistics of nuclear regulatory processes necessitate temporal and spatial regulation of protein-protein and protein-DNA interactions in response to physiological cues. Biochemical, in...

Protein-deoxyribonucleic acid interactions linked to gene expression: ligation-mediated polymerase chain reaction (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

Chromatin immunoprecipitation (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

In situ immunofluorescence analysis: immunofluorescence microscopy (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

Analysis of in vivo gene expression using epitope-tagged proteins (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

Protein-deoxyribonucleic acid interactions linked to gene expression: DNase I digestion (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

Immunofluorescence analysis using epitope-tagged proteins: in vitro system (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

Protein-deoxyribonucleic acid interactions linked to gene expression: electrophoretic mobility shift assay (2004)

Javed, Amjad, Zaidi, Sayyed K., Gutierrez, Soraya E., Lengner, Christopher J., Harrington, Kimberly Stacy, Hovhannisyan, Hayk, ...

Runx2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression (2004)

Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Montecino, Martin A., Javed, Amjad, ...

We present an overview of Runx involvement in regulatory mechanisms that are requisite for fidelity of bone cell growth and differentiation, as well as for skeletal homeostasis and the structural and...

Nuclear microenvironments: an architectural platform for the convergence and integration of transcriptional regulatory signals (2004)

Stein, Gary S., Stein, Janet L., Lian, Jane B., Van Wijnen, Andre J., Montecino, Martin A., Javed, Amjad, ...

Functional interrelationships between the intranuclear organization of nucleic acids and regulatory proteins are obligatory for fidelity of transcriptional activation and repression. In this article,...

Regulatory controls for osteoblast growth and differentiation: role of Runx/Cbfa/AML factors (2004)

Lian, Jane B., Javed, Amjad, Zaidi, Sayyed K., Lengner, Christopher J., Montecino, Martin, Van Wijnen, Andre J., ...

Formation of skeletal elements during embryogenesis and the dynamic remodeling of bone in the adult involve an exquisite interplay of developmental cues, signaling proteins, transcription factors,...

Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription (2004)

Zaidi, Sayyed K., Sullivan, Andrew J., Medina, Ricardo F., Ito, Yoshiaki, Van Wijnen, Andre J., Stein, Janet L., ...

Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of...

Nuclear microenvironments support assembly and organization of the transcriptional regulatory machinery for cell proliferation and differentiation (2004)

Stein, Gary S., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Javed, Amjad, Montecino, Martin A., ...

The temporal and spatial organization of transcriptional regulatory machinery provides microenvironments within the nucleus where threshold concentrations of genes and cognate factors facilitate...

Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells (2003)

Zaidi, Sayyed K., Young, Daniel W., Pockwinse, Shirwin M., Javed, Amjad, Lian, Jane B., Stein, Janet L., ...

Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately...

Identification of HiNF-P, a key activator of cell cycle-controlled histone H4 genes at the onset of S phase (2003)

Mitra, Partha, Xie, Ronglin, Medina, Ricardo F., Hovhannisyan, Hayk, Zaidi, Sayyed K., Wei, Yue, ...

At the G(1)/S phase cell cycle transition, multiple histone genes are expressed to ensure that newly synthesized DNA is immediately packaged as chromatin. Here we have purified and functionally...

Functional architecture of the nucleus: organizing the regulatory machinery for gene expression, replication and repair (2003)

Stein, Gary S., Zaidi, Sayyed K., Braastad, Corey D., Montecino, Martin A., Van Wijnen, Andre J., Choi, Je-Yong, ...

The organization and sorting of regulatory information for transcription, replication and repair depends on components of nuclear architecture. It is necessary, therefore, to understand cellular...

Cell growth regulatory role of Runx2 during proliferative expansion of preosteoblasts (2003)

Pratap, Jitesh, Galindo, Mario, Zaidi, Sayyed K., Vradii, Diana, Bhat, Bheem M., Robinson, John A., ...

The Runx2 (CBFA1/AML3/PEBP2alphaA) transcription factor promotes lineage commitment and differentiation by activating bone phenotypic genes in postproliferative osteoblasts. However, the presence of...

Nuclear microenvironments support physiological control of gene expression (2003)

Stein, Gary S., Lian, Jane B., Montecino, Martin A., Stein, Janet L., Van Wijnen, Andre J., Javed, Amjad, ...

There is growing recognition that the organization of nucleic acids and regulatory proteins is functionally linked to the assembly, localization and activity of gene regulatory machinery. Cellular,...

Runx2/Cbfa1 functions: diverse regulation of gene transcription by chromatin remodeling and co-regulatory protein interactions (2003)

Lian, Jane B., Stein, Janet L., Stein, Gary S., Van Wijnen, Andre J., Montecino, Martin, Javed, Amjad, ...

Development of the osteoblast phenotype requires transcriptional mechanisms that regulate induction of a program of temporally expressed genes. Key components of gene activation, repression, and...

Temporal and spatial parameters of skeletal gene expression: targeting RUNX factors and their coregulatory proteins to subnuclear domains (2003)

Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Choi, Je-Yong, Pratap, Jitesh, ...

Key components of the basal transcription machinery and several tissue-specific transcription factor complexes are functionally compartmentalized as specialized subnuclear domains. We have identified...

Intranuclear organization of RUNX transcriptional regulatory machinery in biological control of skeletogenesis and cancer (2003)

Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Montecino, Martin A., Pratap, Jitesh, ...

RUNX (AML/CBFA/PEBP2) transcription factors serve as paradigms for obligatory relationships between nuclear structure and physiological control of phenotypic gene expression. The RUNX proteins...

Intranuclear trafficking of transcription factors: Requirements for vitamin D-mediated biological control of gene expression (2003)

Stein, Gary S., Lian, Jane B., Stein, Janet L., Van Wijnen, Andre J., Montecino, Martin A., Javed, Amjad, ...

The architecturally associated subnuclear organization of nucleic acids and cognate regulatory factors suggest functional interrelationships between nuclear structure and gene expression. Mechanisms...

Runx2 (Cbfa1, AML-3) interacts with histone deacetylase 6 and represses the p21(CIP1/WAF1) promoter (2002)

Westendorf, Jennifer J., Zaidi, Sayyed K., Cascino, Jonathan E., Kahler, Rachel A., Van Wijnen, Andre J., Lian, Jane B., ...

Runx2 (Cbfa1, AML-3) is multifunctional transcription factor that is essential for osteoblast development. Runx2 binds specific DNA sequences and interacts with transcriptional coactivators and...

Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites (2002)

Zaidi, Sayyed K., Sullivan, Andrew J., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.

Runx factors control lineage commitment and are transcriptional effectors of Smad signaling. Genetic defects in these pathways interfere with normal development. The in situ localization of Runx and...

A specific targeting signal directs Runx2/Cbfa1 to subnuclear domains and contributes to transactivation of the osteocalcin gene (2001)

Zaidi, Sayyed K., Javed, Amjad, Choi, Je-Yong, Van Wijnen, Andre J., Stein, Janet L., Lian, Jane B., ...

Key components of DNA replication and the basal transcriptional machinery as well as several tissue-specific transcription factors are compartmentalized in specialized nuclear domains. In the present...

Subnuclear targeting of Runx/Cbfa/AML factors is essential for tissue-specific differentiation during embryonic development (2001)

Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Zaidi, Sayyed K., Xing, Lianping, Balint, Eva, ...

Runx (Cbfa/AML) transcription factors are critical for tissue-specific gene expression. A unique targeting signal in the C terminus directs Runx factors to discrete foci within the nucleus. Using...

Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites

Zaidi, Sayyed K., Sullivan, Andrew J., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.

Runx factors control lineage commitment and are transcriptional effectors of Smad signaling. Genetic defects in these pathways interfere with normal development. The in situ localization of Runx and...

Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells

Zaidi, Sayyed K., Young, Daniel W., Pockwinse, Shirwin M., Javed, Amjad, Lian, Jane B., Stein, Janet L., ...

Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately...

Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription

Zaidi, Sayyed K, Sullivan, Andrew J, Medina, Ricardo, Ito, Yoshiaki, Van Wijnen, Andre J, Stein, Janet L, ...

Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of...

Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype

Vradii, Diana, Zaidi, Sayyed K., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.

The multifunctional C terminus of the hematopoietic AML1 transcription factor interacts with coregulatory proteins, supports the convergence and integration of physiological signals, and contains the...

The dynamic organization of gene-regulatory machinery in nuclear microenvironments

Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin, ...

Nuclear components are functionally linked with the dynamic temporal and spatial compartmentalization, sorting and integration of regulatory information to facilitate its selective use. For example,...

HiNF-P Directly Links the Cyclin E/CDK2/p220NPAT Pathway to Histone H4 Gene Regulation at the G1/S Phase Cell Cycle Transition

Miele, Angela, Braastad, Corey D., Holmes, William F., Mitra, Partha, Medina, Ricardo, Xie, Ronglin, ...

Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the...

Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites

Zaidi, Sayyed K., Sullivan, Andrew J., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S., Lian, Jane B.

Runx factors control lineage commitment and are transcriptional effectors of Smad signaling. Genetic defects in these pathways interfere with normal development. The in situ localization of Runx and...

Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells

Zaidi, Sayyed K., Young, Daniel W., Pockwinse, Shirwin M., Javed, Amjad, Lian, Jane B., Stein, Janet L., ...

Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately...

Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription

Zaidi, Sayyed K, Sullivan, Andrew J, Medina, Ricardo, Ito, Yoshiaki, Van Wijnen, Andre J, Stein, Janet L, ...

Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of...

Point mutation in AML1 disrupts subnuclear targeting, prevents myeloid differentiation, and effects a transformation-like phenotype

Vradii, Diana, Zaidi, Sayyed K., Lian, Jane B., Van Wijnen, Andre J., Stein, Janet L., Stein, Gary S.

The multifunctional C terminus of the hematopoietic AML1 transcription factor interacts with coregulatory proteins, supports the convergence and integration of physiological signals, and contains the...

HiNF-P Directly Links the Cyclin E/CDK2/p220NPAT Pathway to Histone H4 Gene Regulation at the G1/S Phase Cell Cycle Transition

Miele, Angela, Braastad, Corey D., Holmes, William F., Mitra, Partha, Medina, Ricardo, Xie, Ronglin, ...

Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the...

The dynamic organization of gene-regulatory machinery in nuclear microenvironments

Zaidi, Sayyed K., Young, Daniel W., Choi, Je-Yong, Pratap, Jitesh, Javed, Amjad, Montecino, Martin, ...

Nuclear components are functionally linked with the dynamic temporal and spatial compartmentalization, sorting and integration of regulatory information to facilitate its selective use. For example,...

Mitotic retention of gene expression patterns by the cell fate-determining transcription factor Runx2

Young, Daniel W., Hassan, Mohammad Q., Yang, Xiao-Qing, Galindo, Mario, Javed, Amjad, Zaidi, Sayyed K., ...

During cell division, cessation of transcription is coupled with mitotic chromosome condensation. A fundamental biological question is how gene expression patterns are retained during mitosis to...

Runx2 deficiency and defective subnuclear targeting bypass senescence to promote immortalization and tumorigenic potential

Zaidi, Sayyed K., Pande, Sandhya, Pratap, Jitesh, Gaur, Tripti, Grigoriu, Simina, Ali, Syed A., ...

The osteogenic Runt-related (Runx2) transcription factor negatively regulates proliferation and ribosomal gene expression in normal diploid osteoblasts, but is up-regulated in metastatic breast and...

Phenotypic transcription factors epigenetically mediate cell growth control

Ali, Syed A., Zaidi, Sayyed K., Dacwag, Caroline S., Salma, Nunciada, Young, Daniel W., Shakoori, Abdul R., ...

Ribosomal RNA (rRNA) genes are down-regulated during osteogenesis, myogenesis, and adipogenesis, necessitating a mechanistic understanding of interrelationships between growth control and phenotype...

Structural Coupling of Smad and Runx2 for Execution of the BMP2 Osteogenic Signal*

Javed, Amjad, Bae, Jong-Sup, Afzal, Faiza, Gutierrez, Soraya, Pratap, Jitesh, Zaidi, Sayyed K., ...

Two regulatory pathways, bone morphogenetic protein (BMP)/transforming growth factor-β (TGFβ) and the transcription factor RUNX2, are required for bone formation in vivo. Here we show the...