Scot A. Wolfe

Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites (2008)

Noyes, Marcus B., Christensen, Ryan G., Wakabayashi, Atsuya, Stormo, Gary D., Brodsky, Michael H., Wolfe, Scot A.

We describe the comprehensive characterization of homeodomain DNA-binding specificities from a metazoan genome. The analysis of all 84 independent homeodomains from D. melanogaster reveals the...

Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases (2008)

Meng, Xiangdong, Noyes, Marcus B., Zhu, Lihua J., Lawson, Nathan D., Wolfe, Scot A.

Direct genomic manipulation at a specific locus is still not feasible in most vertebrate model organisms. In vertebrate cell lines, genomic lesions at a specific site have been introduced using...

A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system (2008)

Noyes, Marcus B., Meng, Xiangdong, Wakabayashi, Atsuya, Sinha, Saurabh, Brodsky, Michael H., Wolfe, Scot A.

Specificity data for groups of transcription factors (TFs) in a common regulatory network can be used to computationally identify the location of cis-regulatory modules in a genome. The primary...

A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system (2008)

Noyes, Marcus B., Meng, Xiangdong, Wakabayashi, Atsuya, Sinha, Saurabh, Brodsky, Michael H., Wolfe, Scot A.

Specificity data for groups of transcription factors (TFs) in a common regulatory network can be used to computationally identify the location of cis-regulatory modules in a genome. The primary...

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method (2007)

Meng, Xiangdong, Thibodeau-Beganny, Stacey, Jiang, Tao, Joung, J. Keith, Wolfe, Scot A.

The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify...

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method (2007)

Meng, Xiangdong, Thibodeau-Beganny, Stacey, Jiang, Tao, Joung, J. Keith, Wolfe, Scot A.

The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify...

A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors (2005)

Meng, Xiangdong, Brodsky, Michael H., Wolfe, Scot A.

The DNA-binding specificities of transcription factors can be used to computationally predict cis-regulatory modules (CRMs) that regulate gene expression. However, the absence of specificity data for...

A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors

Meng, Xiangdong, Brodsky, Michael H., Wolfe, Scot A.

The DNA-binding specificities of transcription factors can be used to computationally predict cis-regulatory modules (CRMs) that regulate gene expression1. However, the absence of specificity data...

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method

Meng, Xiangdong, Thibodeau-Beganny, Stacey, Jiang, Tao, Joung, J. Keith, Wolfe, Scot A.

The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify...

A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system

Noyes, Marcus B., Meng, Xiangdong, Wakabayashi, Atsuya, Sinha, Saurabh, Brodsky, Michael H., Wolfe, Scot A.

Specificity data for groups of transcription factors (TFs) in a common regulatory network can be used to computationally identify the location of cis-regulatory modules in a genome. The primary...