Sean Doyle

Nonribosomal peptide synthesis in Aspergillus fumigatus and other fungi (2007)

Stack, Deirdre, Neville, Claire, Doyle, Sean

In fungi, nonribosomal peptide synthetases (NRP synthetases) are large multi-functional enzymes containing adenylation, thiolation (or peptidyl carrier protein, PCP) and condensation domains. These...

Analysis of major intracellular proteins of Aspergillus fumigatus by MALDI mass spectrometry: Identification and characterisation of an elongation factor 1B protein with glutathione transferase activity (2006)

Carberry, Stephen, Neville, Claire M., Kavanagh, Kevin A., Doyle, Sean

Aspergillus fumigatus is a recognised human pathogen, especially in immunocompromised individuals. The availability of the annotated A. fumigatus genome sequence will significantly accelerate our...

The Immune Response to Parvovirus B19 Exposure in previously Seronegative and Seropositive Individuals (2006)

Doyle, Sean, Corcoran, Amanda

Little information is available on the immune response to parvovirus B19 after the administration of contaminated blood products. In the present study, we found that levels of B19 IgG in...

Human Parvovirus B19: Molecular Virology, Clinical Features, Prevalence, Diagnosis and Control (2006)

Corcoran, A., Doyle, Sean

Parvovirus B19 (B19) is an erythrovirus and recent studies have classified B19 as a genotype 1 erythrovirus with genotypes 2 (erythrovirus K71 or A6) and 3 (erythrovirus V9) also present in the human...

Analysis of major intracellular proteins of Aspergillus fumigatus by MALDI mass spectrometry: Identification and characterisation of an elongation factor 1B protein with glutathione transferase activity (2006)

Carberry, Stephen, Neville, Claire M., Kavanagh, Kevin A., Doyle, Sean

Aspergillus fumigatus is a recognised human pathogen, especially in immunocompromised individuals. The availability of the annotated A. fumigatus genome sequence will significantly accelerate our...

The Immune Response to Parvovirus B19 Exposure in previously Seronegative and Seropositive Individuals (2006)

Doyle, Sean, Corcoran, Amanda

Little information is available on the immune response to parvovirus B19 after the administration of contaminated blood products. In the present study, we found that levels of B19 IgG in...

Human Parvovirus B19: Molecular Virology, Clinical Features, Prevalence, Diagnosis and Control (2006)

Corcoran, A., Doyle, Sean

Parvovirus B19 (B19) is an erythrovirus and recent studies have classified B19 as a genotype 1 erythrovirus with genotypes 2 (erythrovirus K71 or A6) and 3 (erythrovirus V9) also present in the human...

The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron (2005)

Reiber, Kathrin, Reeves, Emer P., Neville, Claire M., Winkler, Robert, Gebhardt, Peter, Kavanagh, Kevin, ...

Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C...

The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron (2005)

Reiber, Kathrin, Reeves, Emer P., Neville, Claire M., Winkler, Robert, Gebhardt, Peter, Kavanagh, Kevin, ...

Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C...

Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus (2005)

Burns, Claire, Geraghty, Rachel, Neville, Claire, Murphy, Alan, Kavanagh, Kevin, Doyle, Sean

Analysis of the genome of the human pathogen, Aspergillus fumigatus, revealed the presence of several putative glutathione transferase (GST) open reading frames. Three A. fumigatus GST genes, termed...

A 4’-Phosphopantetheinyl Transferase Mediates Non-Ribosomal Peptide Synthetase Activation in Aspergillus fumigatus (2005)

Neville, Claire, Murphy, Alan, Kavanagh, Kevin, Doyle, Sean

Aspergillus fumigatus is a significant human pathogen. Non-ribosomal peptide (NRP) synthesis is thought to be responsible for a significant proportion of toxin and siderophore production in the...

Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus (2005)

Burns, Claire, Geraghty, Rachel, Neville, Claire, Murphy, Alan, Kavanagh, Kevin, Doyle, Sean

Analysis of the genome of the human pathogen, Aspergillus fumigatus, revealed the presence of several putative glutathione transferase (GST) open reading frames. Three A. fumigatus GST genes, termed...

A 4'-Phosphopantetheinyl Transferase Mediates Non-Ribosomal Peptide Synthetase Activation in Aspergillus fumigatus (2005)

Neville, Claire, Murphy, Alan, Kavanagh, Kevin, Doyle, Sean

Aspergillus fumigatus is a significant human pathogen. Non-ribosomal peptide (NRP) synthesis is thought to be responsible for a significant proportion of toxin and siderophore production in the...

Leucine aminopeptidase of the human blood flukes, Schistosoma mansoni and Schistosoma japonicum (2005)

McCarthy, Elaine, Stack, Colin, Donnelly, Sheila M., Doyle, Sean, Mann, Victoria H., Brindley, Paul J., ...

An array of schistosome endoproteases involved in the digestion of host hemoglobin to absorbable peptides has been described, but the exoprotease responsible for catabolising these peptides to amino...

Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus (2005)

Burns, Claire, Geraghty, Rachel, Neville, Claire, Murphy, Alan, Kavanagh, Kevin, Doyle, Sean

Analysis of the genome of the human pathogen, Aspergillus fumigatus, revealed the presence of several putative glutathione transferase (GST) open reading frames. Three A. fumigatus GST genes, termed...

Leucine aminopeptidase of the human blood flukes, Schistosoma mansoni and Schistosoma japonicum (2005)

McCarthy, Elaine, Stack, Colin, Donnelly, Sheila M., Doyle, Sean, Mann, Victoria H., Brindley, Paul J., ...

An array of schistosome endoproteases involved in the digestion of host hemoglobin to absorbable peptides has been described, but the exoprotease responsible for catabolising these peptides to amino...

B Cell Memory is directed toward conformational epitopes of parvovirus B19 capsid proteins and the unique region of VP1 (2004)

Mahon, Dr. Bernard, Doyle, Dr. Sean, Corcoran, Dr. Amanda

Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established...

Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19 (2004)

Doyle, Dr. Sean, Corcoran, Dr. Amanda

Increased recognition of parvovirus B19(B19), an erythrovirus, as a significant human pathogen that causes fetal loss and severe disease in immunocompromised patients has resulted in intensive...

Detection of Aspergillus fumigatus mycotoxins: immunogen synthesis and immunoassay development (2004)

Doyle, Dr. Sean, Fox, Dr. M., Gray, Dr. G., Kavanagh, Dr. K.

Immunological detection of secreted low molecular weight toxins represents a potentially novel means of diagnosing infection by the fungus Aspergillus fumigatus. Two such metabolites, gliotoxin and...

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1 (2004)

Corcoran, Amanda, Mahon, Bernard P., Doyle, Sean

Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established...

Cathepsin L1, the Major Protease Involved in Liver Fluke (Fasciola hepatica) Virulence (2004)

Collins, Peter R., Stack, Colin M., O'Neill, Sandra M., Doyle, Sean, Ryan, Thecla, Brennan, Gerard P., ...

The secretion and activation of the major cathepsin L1 cysteine protease involved in the virulence of the helminth pathogen Fasciola hepatica was investigated. Only the fully processed and active...

B Cell Memory is directed toward conformational epitopes of parvovirus B19 capsid proteins and the unique region of VP1 (2004)

Mahon, Dr. Bernard, Doyle, Dr. Sean, Corcoran, Dr. Amanda

Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established...

Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19 (2004)

Doyle, Dr. Sean, Corcoran, Dr. Amanda

Increased recognition of parvovirus B19(B19), an erythrovirus, as a significant human pathogen that causes fetal loss and severe disease in immunocompromised patients has resulted in intensive...

Detection of Aspergillus fumigatus mycotoxins: immunogen synthesis and immunoassay development (2004)

Doyle, Dr. Sean, Fox, Dr. M., Gray, Dr. G., Kavanagh, Dr. K.

Immunological detection of secreted low molecular weight toxins represents a potentially novel means of diagnosing infection by the fungus Aspergillus fumigatus. Two such metabolites, gliotoxin and...

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1 (2004)

Corcoran, Amanda, Mahon, Bernard P., Doyle, Sean

Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established...

Cathepsin L1, the Major Protease Involved in Liver Fluke (Fasciola hepatica) Virulence (2004)

Collins, Peter R., Stack, Colin M., O'Neill, Sandra M., Doyle, Sean, Ryan, Thecla, Brennan, Gerard P., ...

The secretion and activation of the major cathepsin L1 cysteine protease involved in the virulence of the helminth pathogen Fasciola hepatica was investigated. Only the fully processed and active...

Cathepsin L1, the major protease involved in liver fluke (fasciola hepatica) virulence : propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells (2004)

Collins, Peter, Dublin City University, Stack, Colin M., Dublin City University, O'Neill, Sandra M., Dublin City University, ...

The secretion and activation of the major cathepsin L1 cysteine protease involved in the virulence of the helminth pathogen Fasciola hepatica was investigated. Only the fully processed and active...

Cathepsin L1, the major protease involved in liver fluke (fasciola hepatica) virulence : propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells (2004)

Collins, Peter, Dublin City University, Stack, Colin M., Dublin City University, O'Neill, Sandra M., Dublin City University, ...

The secretion and activation of the major cathepsin L1 cysteine protease involved in the virulence of the helminth pathogen Fasciola hepatica was investigated. Only the fully processed and active...

Cathepsin L1, the major protease involved in liver fluke (fasciola hepatica) virulence : propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells (2004)

Collins, Peter, Dublin City University, Stack, Colin M., Dublin City University, O'Neill, Sandra M., Dublin City University, ...

The secretion and activation of the major cathepsin L1 cysteine protease involved in the virulence of the helminth pathogen Fasciola hepatica was investigated. Only the fully processed and active...

Cathepsin L1, the major protease involved in liver fluke (fasciola hepatica) virulence : propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells (2004)

Collins, Peter, Dublin City University, University Of Western Sydney, College Of Health And Science, School Of Biomedical And Health Sciences, Stack, Colin M., ...

The secretion and activation of the major cathepsin L1 cysteine protease involved in the virulence of the helminth pathogen Fasciola hepatica was investigated. Only the fully processed and active...

Exposure of the yeast Candida albicans to the anti-neoplastic agent adriamycin increases the tolerance to amphotericin B (2003)

O'Keeffe, Joseph, Doyle, Sean, Kavanagh, Kevin

Cancer patients experience a high incidence of fungal infections due to their immuno-suppressed condition. This work has investigated the interaction of an anti-neoplastic agent, adriamycin...

Exposure of the yeast Candida albicans to the anti-neoplastic agent adriamycin increases the tolerance to amphotericin B (2003)

O'Keeffe, Joseph, Doyle, Sean, Kavanagh, Kevin

Cancer patients experience a high incidence of fungal infections due to their immuno-suppressed condition. This work has investigated the interaction of an anti-neoplastic agent, adriamycin...

Ex Vivo Cytokine responses against Parvovirus B19 Antigens in previously infected pregnant women (2003)

Mahon, Dr. Bernard, Corcoran, Dr. Amanda, McParland, Dr. Peter, Davoren, Dr. Anne, Doyle, Dr. Sean

Parvovirus B19 infection is a significant cause of fetal death. The aim of this study was to investigate the role of maternal immune status in modulating susceptibility to fetal B19 infection....

Ex Vivo Cytokine responses against Parvovirus B19 Antigens in previously infected pregnant women (2003)

Mahon, Dr. Bernard, Corcoran, Dr. Amanda, McParland, Dr. Peter, Davoren, Dr. Anne, Doyle, Dr. Sean

Parvovirus B19 infection is a significant cause of fetal death. The aim of this study was to investigate the role of maternal immune status in modulating susceptibility to fetal B19 infection....

The development of a competitive PCR-ELISA for the detection of equine herpesvirus-1 (2003)

Doyle, Dr. Sean, Daly, Dr. P.

Equine herpesvirus-1 (EHV-1) infection is of significant animal welfare and economic importance. Yet, no standardised molecular techniques are available for diagnosis or confirmation of viral...

The development of a competitive PCR-ELISA for the detection of equine herpesvirus-1 (2003)

Doyle, Dr. Sean, Daly, Dr. P.

Equine herpesvirus-1 (EHV-1) infection is of significant animal welfare and economic importance. Yet, no standardised molecular techniques are available for diagnosis or confirmation of viral...

High-Sensitivity PCR Detection of Parvovirus B19 Plasma (2002)

Doyle, Dr. Sean, Mahon, Dr. Bernard, Corcoran, Dr. A., Daly, Dr. P.

Parvovirus B19 (B19) is a human pathogen transmitted to susceptible individuals via respiratory secretions and contaminated blood or blood products. B19 levels in pooled plasma of less than 104...

High-Sensitivity PCR Detection of Parvovirus B19 Plasma (2002)

Doyle, Dr. Sean, Mahon, Dr. Bernard, Corcoran, Dr. A., Daly, Dr. P.

Parvovirus B19 (B19) is a human pathogen transmitted to susceptible individuals via respiratory secretions and contaminated blood or blood products. B19 levels in pooled plasma of less than 104...

PCR-ELISA detection of Escherichia coli in milk (2002)

Doyle, Dr. Sean, Daly, Dr. P., Collier, Dr. T.

Aims: The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk. Methods and Results: Robust and expeditious DNA extraction and PCR...

PCR-ELISA detection of Escherichia coli in milk (2002)

Doyle, Dr. Sean, Daly, Dr. P., Collier, Dr. T.

Aims: The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk. Methods and Results: Robust and expeditious DNA extraction and PCR...

Baculovirus expression of parvovirus B19 (B19V) NS1: utility in confirming recent infection (2001)

Mahon, Dr. Bernard, Doyle, Dr. Sean, Kavanagh, Dr. Kevin, Corcoran, Dr. A., Ennis, Dr. O.

Background :The presence of anti-parvovirus B19 (B19V) IgM against viral capsid proteins (VP1 and VP2) has long been used to detect recent infection. The utility of antibodies directed against B19V...

Baculovirus expression of parvovirus B19 (B19V) NS1: utility in confirming recent infection (2001)

Mahon, Dr. Bernard, Doyle, Dr. Sean, Kavanagh, Dr. Kevin, Corcoran, Dr. A., Ennis, Dr. O.

Background :The presence of anti-parvovirus B19 (B19V) IgM against viral capsid proteins (VP1 and VP2) has long been used to detect recent infection. The utility of antibodies directed against B19V...

Progressive word hypotheses reduction for very large vocabulary, continuous speech recognition (1997)

Doyle, Sean.

Building on a small vocabulary, speaker independent, continuous speech recognition system, new methods for word hypotheses generation and word lattice pruning are suggested for very large vocabulary...

Progressive word hypotheses reduction for very large vocabulary, continuous speech recognition (1997)

Doyle, Sean.

Building on a small vocabulary, speaker independent, continuous speech recognition system, new methods for word hypotheses generation and word lattice pruning are suggested for very large vocabulary...

Impaired Gamma Interferon Responses against Parvovirus B19 by Recently Infected Children

Corcoran, Amanda, Doyle, Sean, Waldron, David, Nicholson, Alfred, Mahon, Bernard P.

Parvovirus B19 is the causative agent of “fifth disease” of childhood. It has been implicated in a variety of conditions, including unsuccessful pregnancy and rheumatoid arthritis, and is a...

Impaired Gamma Interferon Responses against Parvovirus B19 by Recently Infected Children

Corcoran, Amanda, Doyle, Sean, Waldron, David, Nicholson, Alfred, Mahon, Bernard P.

Parvovirus B19 is the causative agent of “fifth disease” of childhood. It has been implicated in a variety of conditions, including unsuccessful pregnancy and rheumatoid arthritis, and is a...

Proteomic studies in biomedically and industrially relevant fungi

Carberry, Stephen, Doyle, Sean

Historically, the proteomic investigation of filamentous fungi has been restrained by difficulties associated with efficient protein extraction and the lack of extensive fungal genome sequence...