Yoon, Seon-Joo, Ikeda, Shoko, Sadilek, Martin, Hakomori, Sen-itiroh, Ishida, Hideharu, Kiso, Makoto
Aminoceramide mimetic was synthesized and conjugated to N-linked oligosaccharides having multivalent GlcNAc by reductive amination. Ceramide mimetic conjugates with “complex-type” glycan having...
Usui, Hiroko, Hakomori, Sen-Itiroh
Immunotoxins composed of monoclonal antibodies (mAbs) and various toxins have been developed for the treatment of malignancies. We investigated the efficacy of three ricin toxin A-chain...
Characterization of Mouse Integrin {alpha}3 Subunit Gene (1999)
Tsuji, Tsutomu, Han, Seon Ae, Takeuchi, Ken-ichi, Takahashi, Naoki, Hakomori, Sen-itiroh, Irimura, Tatsuro
Integrin α3β1 (VLA-3) is an adhesion receptor for extracellular matrix proteins including various isoforms of laminin. We have isolated mouse genomic clones encoding the integrin α3 subunit and...
Yatomi, Yutaka, Igarashi, Yasuyuki, Hakomori, Sen-itiroh
Gangliosides, highly expressed in the outer leaflet of plasma membranes, mediate a variety of biological processes, including cell-cell and cell-matrix interactions. We examined the effects of...
Functional Role of Glycosphingolipids in Cell Recognition and Signaling (1995)
Hakomori, Sen-itiroh, Igarashi, Yasuyuki
Glycosphingolipids (GSLs), cell type-specific markers which change dramatically during ontogenesis and oncogenesis, have been implicated as playing major roles in cellular interactions and control of...
Genomic organization of human histo-blood group ABO genes (1995)
Yamamoto, Fumi-ichiro, McNeill, Patricia D., Hakomori, Sen-itiroh
We have isolated human genomic DNA clones encompassing 30 kbp of the histo-blood group ABO locus. The locations of the exons have been mapped and the nucleotide sequences of the exon-intron...
Ribonuclease activity of sialic acid-binding lectin from Rana catesbeiana eggs (1993)
Nitta, Kazuo, Oyama, Fumitaka, Oyama, Rieko, Sekiguchi, Kiyotoshi, Kawauchi, Hiroaki, Takayanagi, Yoshio, ...
Sialic acid-binding lectin (SBL) isolated from Rana catesbeiana eggs is a basic protein which agglutinates a large variety of tumour cells and has an amino acid sequence homologous to that of human...
Hozawa, Koji, Wataya, Hideya, Takasaka, Tomonori, Fenderson, Bruce A., Hakomori, Sen-itiroh
Immunohistological examination of guinea pig cochleas was performed using a panel of 25 monoclonal antibodies directed to various lacto-, ganglio- and globo-series carbohydrate epitopes as well as...
Ley antigen expression is correlated with apoptosis (programmed cell death) (1993)
Hiraishi, Katsuya, Suzuki, Keiji, Hakomori, Sen-itiroh, Adachi, Masakazu
Apoptosis (programmed cell death) is a basic physiological process which determines specific patterns of tissue size and shape, and balance of cell number, during morphogenesis, and seems to play an...
A ceramide analogue (PDMP) inhibits glycolipid synthesis in fish embryos (1992)
Fenderson, Bruce A., Ostrander, Gary K., Hausken, Zach, Radin, Norman S., Hakomori, Sen-itiroh
Glycolipids were depleted from medaka embryos using 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an inhibitor of glucosylceramide synthetase. Embryos cultured in the presence of 20 [mu]M...
Kojima, Naoya, Hakomori, Sen-itiroh
GM3-expressing cells adhere, spread and migrate on plastic plates coated with Gg3, LacCer and Gb4, but not with other glycosphingolipids (GSLs). Thus, cell adhesion, spreading and migration through...
Tsuji, Tsutomu, Hakomori, Sen-itiroh, Osawa, Toshiaki
Galactoprotein b3 is one of the cell membrane glycoproteins of fibroblasts showing enhanced expression in association with oncogenic transformation. Analysis of cDNA for this glycoprotein from...
Metoki, Rikiya, Kakudo, Kennichi, Tsuji, Yoshiyuki, Teng, Nelson, Clausen, Henrik, Hakomori, Sen-itiroh
Expression of histo-blood group ABH antigens in 53 cases of ovarian cancer was examined by immunoperoxidase staining of Formalin-fixed as well as frozen histological sections with various monoclonal...
Okada, Yoshio, Radin, Norman S., Hakomori, Sen-itiroh
A culture of BALB/c 3T3 cells grown in the presence of 40 [mu]M of the ceramide analog compound RV538 (2-decanoylamino-3-morpholino-1-phenylpropanol) for several passages caused a substantial...
HOLMES, Eric H., HAKOMORI, Sen-itiroh
The enzyme GDPFuc: GM1 αl↑2fucosyltransferase, induced by chemical carcinogens in precancerous rat liver as well as rat hepatoma cells, was found previously to be membrane bound, and was...
On the Physically Homogeneous, Metal-bound Lipid Complex of Ox Brain (1963)
HAKOMORI, SEN-ITIROH, ISHIMODA, TAIKO, KAWAUCHI, HIROAKI, NAKAMURA, KIYOSHI
Physically distinguishable microdomains associated with various functional membrane proteins are one of the major current topics in cell biology. Glycosphingolipids present in such microdomains have...
Yu, Song, Kojima, Naoya, Hakomori, Sen-itiroh, Kudo, Shigeharu, Inoue, Sadako, Inoue, Yasuo
KDNα2→3Galβ4Glcβ1Cer [(KDN)GM3] is a major (≈90%) component of total gangliosides found in sperm of rainbow trout (Oncorhynchus mykiss) and was shown to be present prominently at the sperm...
The glycolipids of normal fibroblasts cells at different stages of growth and with differing degrees of susceptibility to contact inhibition have been analyzed, as well as the glycolipids of virally...
Fukuda, Minoru, Fukuda, Michiko N., Papayannopoulou, Thalia, Hakomori, Sen-itiroh
Human erythroblasts in culture, irrespective of the ontogenic stage of their progenitors, are characterized by: (i) the barely detectable amount of band 3 glycoprotein, (ii) the presence of two...
Gahmberg, Carl Gustav, Hakomori, Sen-Itiroh
By use of galactose oxidase (EC 1.1.3.9), followed by reduction with tritiated sodium borohydride, the surface structures of transformed 3T3 and NIL cells, under ordinary growth conditions, were...
Physically distinguishable microdomains associated with various functional membrane proteins are one of the major current topics in cell biology. Glycosphingolipids present in such microdomains have...
Yu, Song, Kojima, Naoya, Hakomori, Sen-itiroh, Kudo, Shigeharu, Inoue, Sadako, Inoue, Yasuo
KDNα2→3Galβ4Glcβ1Cer [(KDN)GM3] is a major (≈90%) component of total gangliosides found in sperm of rainbow trout (Oncorhynchus mykiss) and was shown to be present prominently at the sperm...
The glycolipids of normal fibroblasts cells at different stages of growth and with differing degrees of susceptibility to contact inhibition have been analyzed, as well as the glycolipids of virally...
Fukuda, Minoru, Fukuda, Michiko N., Papayannopoulou, Thalia, Hakomori, Sen-itiroh
Human erythroblasts in culture, irrespective of the ontogenic stage of their progenitors, are characterized by: (i) the barely detectable amount of band 3 glycoprotein, (ii) the presence of two...
Gahmberg, Carl Gustav, Hakomori, Sen-Itiroh
By use of galactose oxidase (EC 1.1.3.9), followed by reduction with tritiated sodium borohydride, the surface structures of transformed 3T3 and NIL cells, under ordinary growth conditions, were...
Yoon, Seon-Joo, Nakayama, Ken-ichi, Hikita, Toshiyuki, Handa, Kazuko, Hakomori, Sen-itiroh
Epidermal growth factor receptor (EGFR) at membrane microdomains plays an essential role in the growth control of epidermal cells, including cancer cells derived therefrom. Ligand-dependent...
Todeschini, Adriane Regina, Dos Santos, Jose Nilson, Handa, Kazuko, Hakomori, Sen-itiroh
Ganglioside GM2 complexed with tetraspanin CD82 in glycosynaptic microdomain of HCV29 and other epithelial cells inhibits hepatocyte growth factor-induced cMet tyrosine kinase. In addition, adhesion...
Guan, Feng, Handa, Kazuko, Hakomori, Sen-itiroh
Epithelial-to-mesenchymal cell transition (EMT) is a basic process in embryonic development and cancer progression. The present study demonstrates involvement of glycosphingolipids (GSLs) in the EMT...