Seng Gee Lim

Cellular Characterization of SARS Coronavirus Nucleocapsid (2004)

Goh, Phuay-Yee, Choi, Yook-Wah, Shuo, Shen, Tan, Yee-Joo, Fielding, Burtram, Tan, Timothy H. P., ...

The Severe and Acute Respiratory Syndrome coronavirus (SARS CoV) is a newly-emerged virus that caused an outbreak of atypical pneumonia in the winter of 2002-2003. Polyclonal antibodies raised...

Histone H4 histidine kinase displays the expression pattern of a liver oncodevelopmental marker (2004)

Tan, Eiling, Besant, Paul G., Zu, Xin Lin, Turck, Christoph W., Bogoyevitch, Marie A., Lim, Seng Gee, ...

Protein phosphorylation is a vital process in the regulation of mammalian cell division and the protein kinases that catalyze the phosphorylation of proteins on serine, threonine and tyrosine...

The gut in HIV infection (1993)

Lim, Seng Gee.

Submitted in fulfilment for the degree of Doctor of Philosophy, Faculty of Medicine. Summary: leaves xiv-xvii. Typescript. Includes bibliographical references (leaves 214-255)

Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase

Khu, Yee-Ling, Koh, Esther, Lim, Siew Pheng, Tan, Yin Hwee, Brenner, Sydney, Lim, Seng Gee, ...

Interaction between viral proteins is necessary for viral replication and viral particle assembly. We used the yeast two-hybrid assay to identify interactions among all the mature proteins of the...

Cellular RNA Helicase p68 Relocalization and Interaction with the Hepatitis C Virus (HCV) NS5B Protein and the Potential Role of p68 in HCV RNA Replication

Goh, Phuay-Yee, Tan, Yee-Joo, Lim, Siew Pheng, Tan, Y. H., Lim, Seng Gee, Fuller-Pace, Frances, ...

Chronic infection by hepatitis C virus (HCV) can lead to severe hepatitis and cirrhosis and is closely associated with hepatocellular carcinoma. The replication cycle of HCV is poorly understood but...

Characterization of a Unique Group-Specific Protein (U122) of the Severe Acute Respiratory Syndrome Coronavirus

Fielding, Burtram C., Tan, Yee-Joo, Shuo, Shen, Tan, Timothy H. P., Ooi, Eng-Eong, Lim, Seng Gee, ...

A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and...

Overexpression of 7a, a Protein Specifically Encoded by the Severe Acute Respiratory Syndrome Coronavirus, Induces Apoptosis via a Caspase-Dependent Pathway

Tan, Yee-Joo, Fielding, Burtram C., Goh, Phuay-Yee, Shen, Shuo, Tan, Timothy H. P., Lim, Seng Gee, ...

Besides genes that are homologous to proteins found in other coronaviruses, the severe acute respiratory syndrome coronavirus genome also contains nine other potential open reading frames....

Amino Acids 1055 to 1192 in the S2 Region of Severe Acute Respiratory Syndrome Coronavirus S Protein Induce Neutralizing Antibodies: Implications for the Development of Vaccines and Antiviral Agents

Keng, Choong-Tat, Zhang, Aihua, Shen, Shuo, Lip, Kuo-Ming, Fielding, Burtram C., Tan, Timothy H. P., ...

The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) interacts with cellular receptors to mediate membrane fusion, allowing viral entry into host cells; hence it is...

The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells

Tan, Yee-Joo, Tham, Puay-Yoke, Chan, Daphne Z. L., Chou, Chih-Fong, Shen, Shuo, Fielding, Burtram C., ...

Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung...

Genetic lesions within the 3a gene of SARS-CoV

Tan, Timothy HP, Barkham, Timothy, Fielding, Burtram C, Chou, Chih-Fong, Shen, Shuo, Lim, Seng Gee, ...

A series of frameshift mutations within the 3a gene has been observed in culture-derived severe acute respiratory syndrome coronavirus (SARS-CoV). We report here that viral RNA from clinical samples...

Monoclonal Antibodies Targeting the HR2 Domain and the Region Immediately Upstream of the HR2 of the S Protein Neutralize In Vitro Infection of Severe Acute Respiratory Syndrome Coronavirus

Lip, Kuo-Ming, Shen, Shuo, Yang, Xiaoming, Keng, Choong-Tat, Zhang, Aihua, Oh, Hsueh-Ling Janice, ...

We have previously shown that an Escherichia coli-expressed, denatured spike (S) protein fragment of the severe acute respiratory coronavirus, containing residues 1029 to 1192 which include the...

Randomized, Double-Blind Study of Emtricitabine (FTC) plus Clevudine versus FTC Alone in Treatment of Chronic Hepatitis B

Lim, Seng Gee, Krastev, Zahary, Ng, Tay Meng, Mechkov, Grigor, Kotzev, Iskren Andreev, Chan, Sing, ...

Emtricitabine (FTC) is approved for the treatment of human immunodeficiency virus. FTC and clevudine (CLV) have activity against hepatitis B virus (HBV). This report summarizes the results of a...

Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase

Khu, Yee-Ling, Koh, Esther, Lim, Siew Pheng, Tan, Yin Hwee, Brenner, Sydney, Lim, Seng Gee, ...

Interaction between viral proteins is necessary for viral replication and viral particle assembly. We used the yeast two-hybrid assay to identify interactions among all the mature proteins of the...

Cellular RNA Helicase p68 Relocalization and Interaction with the Hepatitis C Virus (HCV) NS5B Protein and the Potential Role of p68 in HCV RNA Replication

Goh, Phuay-Yee, Tan, Yee-Joo, Lim, Siew Pheng, Tan, Y. H., Lim, Seng Gee, Fuller-Pace, Frances, ...

Chronic infection by hepatitis C virus (HCV) can lead to severe hepatitis and cirrhosis and is closely associated with hepatocellular carcinoma. The replication cycle of HCV is poorly understood but...

Characterization of a Unique Group-Specific Protein (U122) of the Severe Acute Respiratory Syndrome Coronavirus

Fielding, Burtram C., Tan, Yee-Joo, Shuo, Shen, Tan, Timothy H. P., Ooi, Eng-Eong, Lim, Seng Gee, ...

A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and...

Overexpression of 7a, a Protein Specifically Encoded by the Severe Acute Respiratory Syndrome Coronavirus, Induces Apoptosis via a Caspase-Dependent Pathway

Tan, Yee-Joo, Fielding, Burtram C., Goh, Phuay-Yee, Shen, Shuo, Tan, Timothy H. P., Lim, Seng Gee, ...

Besides genes that are homologous to proteins found in other coronaviruses, the severe acute respiratory syndrome coronavirus genome also contains nine other potential open reading frames....

Amino Acids 1055 to 1192 in the S2 Region of Severe Acute Respiratory Syndrome Coronavirus S Protein Induce Neutralizing Antibodies: Implications for the Development of Vaccines and Antiviral Agents

Keng, Choong-Tat, Zhang, Aihua, Shen, Shuo, Lip, Kuo-Ming, Fielding, Burtram C., Tan, Timothy H. P., ...

The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) interacts with cellular receptors to mediate membrane fusion, allowing viral entry into host cells; hence it is...

The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells

Tan, Yee-Joo, Tham, Puay-Yoke, Chan, Daphne Z. L., Chou, Chih-Fong, Shen, Shuo, Fielding, Burtram C., ...

Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung...

Genetic lesions within the 3a gene of SARS-CoV

Tan, Timothy HP, Barkham, Timothy, Fielding, Burtram C, Chou, Chih-Fong, Shen, Shuo, Lim, Seng Gee, ...

A series of frameshift mutations within the 3a gene has been observed in culture-derived severe acute respiratory syndrome coronavirus (SARS-CoV). We report here that viral RNA from clinical samples...

Monoclonal Antibodies Targeting the HR2 Domain and the Region Immediately Upstream of the HR2 of the S Protein Neutralize In Vitro Infection of Severe Acute Respiratory Syndrome Coronavirus

Lip, Kuo-Ming, Shen, Shuo, Yang, Xiaoming, Keng, Choong-Tat, Zhang, Aihua, Oh, Hsueh-Ling Janice, ...

We have previously shown that an Escherichia coli-expressed, denatured spike (S) protein fragment of the severe acute respiratory coronavirus, containing residues 1029 to 1192 which include the...

Randomized, Double-Blind Study of Emtricitabine (FTC) plus Clevudine versus FTC Alone in Treatment of Chronic Hepatitis B

Lim, Seng Gee, Krastev, Zahary, Ng, Tay Meng, Mechkov, Grigor, Kotzev, Iskren Andreev, Chan, Sing, ...

Emtricitabine (FTC) is approved for the treatment of human immunodeficiency virus. FTC and clevudine (CLV) have activity against hepatitis B virus (HBV). This report summarizes the results of a...

The Level of Viral Antigen Presented by Hepatocytes Influences CD8 T-Cell Function▿

Gehring, Adam J., Sun, Dianxing, Kennedy, Patrick T. F., Lim, Seng Gee, Wasser, Shanthi, ...

CD8 T cells exert their antiviral function through cytokines and lysis of infected cells. Because hepatocytes are susceptible to noncytolytic mechanisms of viral clearance, CD8 T-cell antiviral...

Host Ethnicity and Virus Genotype Shape the Hepatitis B Virus-Specific T-Cell Repertoire ▿ †

Tan, Anthony Tanoto, Loggi, Elisabetta, Boni, Carolina, Chia, Adeline, Gehring, Adam J., Sastry, Konduru S. R., ...

Repertoire composition, quantity, and qualitative functional ability are the parameters that define virus-specific T-cell responses and are linked with their potential to control infection. We took...

The economics of treating chronic hepatitis B in Asia

Dan, Yock Young, Aung, Myat Oo, Lim, Seng Gee

Chronic hepatitis B constitutes a significant health and economic burden to Asian countries. Six medications are now approved for the treatment of chronic hepatitis B, but there is still significant...