Seppo Parkkila

Expression of iron-related genes in human brain and brain tumors (2009)

Hänninen, Milla M, Haapasalo, Joonas, Haapasalo, Hannu, Fleming, Robert E, Britton, Robert S, Bacon, Bruce R, ...

Abstract Background Defective iron homeostasis may be involved in the development of some diseases within the central nervous system. Although the expression of genes involved in normal iron balance...

Modification of carbonic anhydrase II with acetaldehyde, the first metabolite of ethanol, leads to decreased enzyme activity (2008)

Bootorabi, Fatemeh, Jänis, Janne, Valjakka, Jarkko, Isoniemi, Sari, Vainiotalo, Pirjo, Vullo, Daniela, ...

Abstract Background Acetaldehyde, the first metabolite of ethanol, can generate covalent modifications of proteins and cellular constituents. However, functional consequences of such modification...

The calcium-binding protein S100P in normal and malignant human tissues (2008)

Parkkila, Seppo, Pan, Pei-wen, Ward, Aoife, Gibadulinova, Adriana, Oveckova, Ingrid, Pastorekova, Silvia, ...

Abstract Background S100P is a Ca 2+ binding protein overexpressed in a variety of cancers, and thus, has been considered a potential tumor biomarker. Very little has been studied about its normal...

Carbonic anhydrase IX in oligodendroglial brain tumors (2008)

Järvelä, Sally, Parkkila, Seppo, Bragge, Helena, Kähkönen, Marketta, Parkkila, Anna-Kaisa, Soini, Ylermi, ...

Abstract Background Carbonic anhydrase IX is a hypoxia-induced enzyme that has many biologically important functions, including its role in cell adhesion and invasion. Methods This study was set out...

Identification of an alternatively spliced isoform of carbonic anhydrase XII in diffusely infiltrating astrocytic gliomas (2008)

Haapasalo, Joonas, Hilvo, Mika, Nordfors, Kristiina, Haapasalo, Hannu, Parkkila, Seppo, Hyrskyluoto, Alise, ...

Carbonic anhydrase XII (CA XII) is a transmembrane enzyme that is associated with neoplastic growth. CA XII has been proposed to be involved in acidification of the extracellular milieu, creating an...

Effects of iron loading on muscle: genome-wide mRNA expression profiling in the mouse (2007)

Rodriguez, Alejandra, Hilvo, Mika, Kytömäki, Leena, Fleming, Robert E, Britton, Robert S, Bacon, Bruce R, ...

Abstract Background Hereditary hemochromatosis (HH) encompasses genetic disorders of iron overload characterized by deficient expression or function of the iron-regulatory hormone hepcidin. Mutations...

Carbonic anhydrase II in the endothelium of glial tumors: A potential target for therapy (2007)

Haapasalo, Joonas, Nordfors, Kristiina, Järvelä, Sally, Bragge, Helena, Rantala, Immo, Parkkila, Anna-Kaisa, ...

Carbonic anhydrase isozyme II (CA II) is a cytosolic enzyme that is highly expressed in most organs, including the brain, where it is mainly located in the oligodendrocytes. Recent studies have shown...

A systematic quantification of carbonic anhydrase transcripts in the mouse digestive system (2007)

Pan, Pei-wen, Rodriguez, Alejandra, Parkkila, Seppo

Abstract Background Carbonic anhydrases (CAs) are physiologically important enzymes which participate in many gastrointestinal processes such as acid and bicarbonate secretion and metabolic pathways...

Expression of carbonic anhydrases IX and XII during mouse embryonic development (2006)

Kallio, Heini, Pastorekova, Silvia, Pastorek, Jaromir, Waheed, Abdul, Sly, William S, Mannisto, Susanna, ...

Abstract Background Of the thirteen active carbonic anhydrase (CA) isozymes, CA IX and XII have been linked to carcinogenesis. It has been suggested that these membrane-bound CAs participate in...

Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa (2005)

Kummola, Laura, Hämäläinen, Jonna M, Kivelä, Jyrki, Kivelä, Antti J, Saarnio, Juha, Karttunen, Tuomo, ...

Abstract Background Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer...

Transmembrane carbonic anhydrase isozymes IX and XII in the female mouse reproductive organs (2004)

Hynninen, Piritta, Hämäläinen, Jonna M, Pastorekova, Silvia, Pastorek, Jaromir, Waheed, Abdul, Sly, William S, ...

Abstract Background Carbonic anhydrase (CA) classically catalyses the reversible hydration of dissolved CO 2 to form bicarbonate ions and protons. The twelve active CA isozymes are thought to...

The plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV (2002)

Parkkila, Seppo, Kivelä, Antti J, Kaunisto, Kari, Parkkila, Anna-Kaisa, Hakkola, Jukka, Rajaniemi, Hannu, ...

Abstract Background Biochemical and histochemical studies have both previously indicated plasma membrane-associated carbonic anhydrase (CA) activity in hepatocytes which has been assumed to be CA IV....

Expression of the transmembrane carbonic anhydrases, CA IX and CA XII, in the human male excurrent ducts (2001)

Karhumaa, Pepe, Kaunisto, Kari, Parkkila, Seppo, Waheed, Abdul, Pastoreková, Silvia, Pastorek, Jaromir, ...

Testicular fluid is concentrated and acidified during its passage through the excurrent ducts. These processes involve bicarbonate absorption, in which carbonic anhydrases are implicated. In this...

Recent advances in the pathology of alcoholic myopathy (2001)

Preedy, Victor R., Adachi, Junko, Peters, Timothy J., Worrall, Simon, Parkkila, Seppo, Niemela, Onni, ...

This article represents the proceedings of a symposium at the 2000 ISBRA Meeting in Yokohama, Japan. The chairs were Victor R. Preedy and Junko Adachi. The presentations were (1) Alcoholic myopathy:...

EVIDENCE OF ACETALDEHYDE-PROTEIN ADDUCT FORMATION IN RAT BRAIN AFTER LIFELONG CONSUMPTION OF ETHANOL (2000)

Rintala, Jyrki, Jaatinen, Pia, Parkkila, Seppo, Sarviharju, Maija, Kiianmaa, Kalervo, Hervonen, Antti, ...

Acetaldehyde, the first metabolite of ethanol, has been shown to be capable of binding covalently to liver proteins in vivo, which may be responsible for a variety of toxic effects of ethanol....

The impact of Active Networks on established Network Operators. (1999)

Juhola , Arto, Marshall, Ian, Covaci, Stefan, Thomas , Velte, Donohoe, Mike, Parkkila, Seppo

A collaborative case based study has established that Active Networks will have a very significant impact on Network operators. Active Networking appears to be the only route to adding integrated...

Association of HFE protein with transferrin receptor in crypt enterocytes of human duodenum

Waheed, Abdul, Parkkila, Seppo, Saarnio, Juha, Fleming, Robert E., Zhou, Xiao Yan, Tomatsu, Shunji, ...

In hereditary hemochromatosis (HH), intestinal absorption of dietary iron is increased, leading to excessive iron accumulation in tissues and resultant organ damage. The HFE protein, which is...

Carbonic anhydrase inhibitor suppresses invasion of renal cancer cells in vitro

Parkkila, Seppo, Rajaniemi, Hannu, Parkkila, Anna-Kaisa, Kivelä, Jyrki, Waheed, Abdul, Pastoreková, Silvia, ...

Acidification of the extracellular milieu of malignant tumors is reported to increase the invasive behavior of cancer cells. In normal tissues, production of acid is catalyzed by carbonic anhydrases...

HFE gene knockout produces mouse model of hereditary hemochromatosis

Zhou, Xiao Yan, Tomatsu, Shunji, Fleming, Robert E., Parkkila, Seppo, Waheed, Abdul, Jiang, Jinxing, ...

Hereditary hemochromatosis (HH) is a common autosomal recessive disease characterized by increased iron absorption and progressive iron storage that results in damage to major organs in the body....

Immunohistochemistry of HLA-H, the protein defective in patients with hereditary hemochromatosis, reveals unique pattern of expression in gastrointestinal tract

Parkkila, Seppo, Waheed, Abdul, Britton, Robert S., Feder, John N., Tsuchihashi, Zenta, Schatzman, Randall C., ...

Hereditary hemochromatosis (HH) is a common autosomal recessive disorder of iron metabolism that leads to excessive iron storage in the liver and other organs. Recently, between 83 and 100% of HH...

Association of the transferrin receptor in human placenta with HFE, the protein defective in hereditary hemochromatosis

Parkkila, Seppo, Waheed, Abdul, Britton, Robert S., Bacon, Bruce R., Zhou, Xiao Yan, Tomatsu, Shunji, ...

Hereditary hemochromatosis (HH) is a common autosomal recessive disease associated with loss of regulation of dietary iron absorption and excessive iron deposition in major organs of the body....

Hereditary hemochromatosis: Effects of C282Y and H63D mutations on association with β2-microglobulin, intracellular processing, and cell surface expression of the HFE protein in COS-7 cells

Waheed, Abdul, Parkkila, Seppo, Zhou, Xiao Yan, Tomatsu, Shunji, Tsuchihashi, Zenta, Feder, John N., ...

Hereditary hemochromatosis (HH) is the most common autosomal recessive disorder known in humans. A candidate gene for HH called HFE has recently been cloned that encodes a novel member of the major...

Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain

Parkkila, Seppo, Parkkila, Anna-Kaisa, Rajaniemi, Hannu, Shah, Gul N., Grubb, Jeffrey H., Waheed, Abdul, ...

Although long suspected from histochemical evidence for carbonic anhydrase (CA) activity on neurons and observations that CA inhibitors enhance the extracellular alkaline shifts associated with...

The identification of secreted carbonic anhydrase VI as a constitutive glycoprotein of human and rat milk

Karhumaa, Pepe, Leinonen, Jukka, Parkkila, Seppo, Kaunisto, Kari, Tapanainen, Juha, Rajaniemi, Hannu

In addition to essential nutrients, human milk contains several classes of bioactive factors such as enzymes, hormones, and growth factors, many of which are implicated in infantile growth and...

Folate deficiency disturbs hepatic methionine metabolism and promotes liver injury in the ethanol-fed micropig

Halsted, Charles H., Villanueva, Jesus A., Devlin, Angela M., Niemelä, Onni, Parkkila, Seppo, Garrow, Timothy A., ...

Alcoholic liver disease is associated with abnormal hepatic methionine metabolism and folate deficiency. Because folate is integral to the methionine cycle, its deficiency could promote alcoholic...

Carbonic anhydrase isozyme-II-deficient mice lack the duodenal bicarbonate secretory response to prostaglandin E2

Leppilampi, Mari, Parkkila, Seppo, Karttunen, Tuomo, Gut, Marta Ortova, Gros, Gerolf, Sjöblom, Markus

Duodenal bicarbonate secretion (DBS) is accepted as the primary mucosal defense against acid discharged from the stomach and is impaired in patients with duodenal ulcer disease. The secretory...

Characterization of CA XV, a new GPI-anchored form of carbonic anhydrase

Hilvo, Mika, Tolvanen, Martti, Clark, Amy, Shen, Bairong, Shah, Gul N., Waheed, Abdul, ...

The main function of CAs (carbonic anhydrases) is to participate in the regulation of acid–base balance. Although 12 active isoenzymes of this family had already been described, analyses of genomic...

Association of HFE protein with transferrin receptor in crypt enterocytes of human duodenum

Waheed, Abdul, Parkkila, Seppo, Saarnio, Juha, Fleming, Robert E., Zhou, Xiao Yan, Tomatsu, Shunji, ...

In hereditary hemochromatosis (HH), intestinal absorption of dietary iron is increased, leading to excessive iron accumulation in tissues and resultant organ damage. The HFE protein, which is...

Carbonic anhydrase inhibitor suppresses invasion of renal cancer cells in vitro

Parkkila, Seppo, Rajaniemi, Hannu, Parkkila, Anna-Kaisa, Kivelä, Jyrki, Waheed, Abdul, Pastoreková, Silvia, ...

Acidification of the extracellular milieu of malignant tumors is reported to increase the invasive behavior of cancer cells. In normal tissues, production of acid is catalyzed by carbonic anhydrases...

HFE gene knockout produces mouse model of hereditary hemochromatosis

Zhou, Xiao Yan, Tomatsu, Shunji, Fleming, Robert E., Parkkila, Seppo, Waheed, Abdul, Jiang, Jinxing, ...

Hereditary hemochromatosis (HH) is a common autosomal recessive disease characterized by increased iron absorption and progressive iron storage that results in damage to major organs in the body....

Immunohistochemistry of HLA-H, the protein defective in patients with hereditary hemochromatosis, reveals unique pattern of expression in gastrointestinal tract

Parkkila, Seppo, Waheed, Abdul, Britton, Robert S., Feder, John N., Tsuchihashi, Zenta, Schatzman, Randall C., ...

Hereditary hemochromatosis (HH) is a common autosomal recessive disorder of iron metabolism that leads to excessive iron storage in the liver and other organs. Recently, between 83 and 100% of HH...

Association of the transferrin receptor in human placenta with HFE, the protein defective in hereditary hemochromatosis

Parkkila, Seppo, Waheed, Abdul, Britton, Robert S., Bacon, Bruce R., Zhou, Xiao Yan, Tomatsu, Shunji, ...

Hereditary hemochromatosis (HH) is a common autosomal recessive disease associated with loss of regulation of dietary iron absorption and excessive iron deposition in major organs of the body....

Hereditary hemochromatosis: Effects of C282Y and H63D mutations on association with β2-microglobulin, intracellular processing, and cell surface expression of the HFE protein in COS-7 cells

Waheed, Abdul, Parkkila, Seppo, Zhou, Xiao Yan, Tomatsu, Shunji, Tsuchihashi, Zenta, Feder, John N., ...

Hereditary hemochromatosis (HH) is the most common autosomal recessive disorder known in humans. A candidate gene for HH called HFE has recently been cloned that encodes a novel member of the major...

Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain

Parkkila, Seppo, Parkkila, Anna-Kaisa, Rajaniemi, Hannu, Shah, Gul N., Grubb, Jeffrey H., Waheed, Abdul, ...

Although long suspected from histochemical evidence for carbonic anhydrase (CA) activity on neurons and observations that CA inhibitors enhance the extracellular alkaline shifts associated with...

The identification of secreted carbonic anhydrase VI as a constitutive glycoprotein of human and rat milk

Karhumaa, Pepe, Leinonen, Jukka, Parkkila, Seppo, Kaunisto, Kari, Tapanainen, Juha, Rajaniemi, Hannu

In addition to essential nutrients, human milk contains several classes of bioactive factors such as enzymes, hormones, and growth factors, many of which are implicated in infantile growth and...

Folate deficiency disturbs hepatic methionine metabolism and promotes liver injury in the ethanol-fed micropig

Halsted, Charles H., Villanueva, Jesus A., Devlin, Angela M., Niemelä, Onni, Parkkila, Seppo, Garrow, Timothy A., ...

Alcoholic liver disease is associated with abnormal hepatic methionine metabolism and folate deficiency. Because folate is integral to the methionine cycle, its deficiency could promote alcoholic...

Carbonic anhydrase isozyme-II-deficient mice lack the duodenal bicarbonate secretory response to prostaglandin E2

Leppilampi, Mari, Parkkila, Seppo, Karttunen, Tuomo, Gut, Marta Ortova, Gros, Gerolf, Sjöblom, Markus

Duodenal bicarbonate secretion (DBS) is accepted as the primary mucosal defense against acid discharged from the stomach and is impaired in patients with duodenal ulcer disease. The secretory...

Characterization of CA XV, a new GPI-anchored form of carbonic anhydrase

Hilvo, Mika, Tolvanen, Martti, Clark, Amy, Shen, Bairong, Shah, Gul N., Waheed, Abdul, ...

The main function of CAs (carbonic anhydrases) is to participate in the regulation of acid–base balance. Although 12 active isoenzymes of this family had already been described, analyses of genomic...

Carbonic anhydrase gene expression in CA II-deficient (Car2−/−) and CA IX-deficient (Car9−/−) mice

Pan, Peiwen, Leppilampi, Mari, Pastorekova, Silvia, Pastorek, Jaromir, Waheed, Abdul, Sly, William S, ...

Using real-time PCR and immunohistochemistry, we have examined the expression of carbonic anhydrase isozymes (CA) I, II, III, IV, IX, XII, XIII and XIV in the brain, kidney, stomach and colon of the...

Immunohistochemical Study of Colorectal Tumors for Expression of a Novel Transmembrane Carbonic Anhydrase, MN/CA IX, with Potential Value as a Marker of Cell Proliferation

Saarnio, Juha, Parkkila, Seppo, Parkkila, Anna-Kaisa, Haukipuro, Kari, Pastoreková, Silvia, Pastorek, Jaromir, ...

Carbonic anhydrase isoenzyme IX, MN/CA IX, is a recently discovered member of the carbonic anhydrase (CA) gene family with a suggested function in acid-base balance, intercellular communication, and...

Expression of a Novel Transmembrane Carbonic Anhydrase Isozyme XII in Normal Human Gut and Colorectal Tumors

Kivelä, Antti, Parkkila, Seppo, Saarnio, Juha, Karttunen, Tuomo J., Kivelä, Jyrki, Parkkila, Anna-Kaisa, ...

Carbonic anhydrase isozyme XII is a recently discovered member of the α-carbonic anhydrase gene family with a suggested role in von Hippel-Lindau gene-mediated carcinogenesis. Increased expression...

Salivary carbonic anhydrase isoenzyme VI

Kivelä, Jyrki, Parkkila, Seppo, Parkkila, Anna-Kaisa, Leinonen, Jukka, Rajaniemi, Hannu

The carbonic anhydrases (CAs) participate in the maintenance of pH homeostasis in various tissues and biological fluids of the human body by catalysing the reversible reaction CO2+ H2O ⇌ HCO3−+...

Carbonic anhydrase II in the endothelium of glial tumors: A potential target for therapy

Haapasalo, Joonas, Nordfors, Kristiina, Järvelä, Sally, Bragge, Helena, Rantala, Immo, Parkkila, Anna-Kaisa, ...

Carbonic anhydrase isozyme II (CA II) is a cytosolic enzyme that is highly expressed in most organs, including the brain, where it is mainly located in the oligodendrocytes. Recent studies have shown...

Identification of an alternatively spliced isoform of carbonic anhydrase XII in diffusely infiltrating astrocytic gliomas

Haapasalo, Joonas, Hilvo, Mika, Nordfors, Kristiina, Haapasalo, Hannu, Parkkila, Seppo, Hyrskyluoto, Alise, ...

Carbonic anhydrase XII (CA XII) is a transmembrane enzyme that is associated with neoplastic growth. CA XII has been proposed to be involved in acidification of the extracellular milieu, creating an...

Global Transcriptional Response to Hfe Deficiency and Dietary Iron Overload in Mouse Liver and Duodenum

Rodriguez, Alejandra, Luukkaala, Tiina, Fleming, Robert E., Britton, Robert S., Bacon, Bruce R., Parkkila, Seppo

Iron is an essential trace element whose absorption is usually tightly regulated in the duodenum. HFE-related hereditary hemochromatosis (HH) is characterized by abnormally low expression of the...