Sergey A. Lukyanov

Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crab (2008)

Anisimova, Veronika E, Rebrikov, Denis V, Shagin, Dmitry A, Kozhemyako, Valery B, Menzorova, Natalia I, Staroverov, Dmitry B, ...

Abstract Background Nucleases, which are key components of biologically diverse processes such as DNA replication, repair and recombination, antiviral defense, apoptosis and digestion, have...

GFP-Like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity (2004)

Shagin, Dmitry A., Barsova, Ekaterina V., Yanushevich, Yurii G., Fradkov, Arkady F., Lukyanov, Konstantin A., Labas, Yulii A., ...

Homologs of the green fluorescent protein (GFP), including the recently described GFP-like domains of certain extracellular matrix proteins in Bilaterian organisms, are remarkably similar at the...

GFP-like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity (2004)

Shagin, Dmitry A., Barsova, Ekaterina V., Yanushevich, Yurii G., Fradkov, Arkady F., Lukyanov, Konstantin A., Labas, Yulii A., ...

Homologs of the green fluorescent protein (GFP), including the recently described GFP-like domains of certain extracellular matrix proteins in Bilaterian organisms, are remarkably similar at the...

Simple cDNA normalization using kamchatka crab duplex-specific nuclease (2004)

Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., ...

We developed a novel simple cDNA normalization method [termed duplex‐specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full‐length cDNA...

GFP-Like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity (2004)

Shagin, Dmitry A., Barsova, Ekaterina V., Yanushevich, Yurii G., Fradkov, Arkady F., Lukyanov, Konstantin A., Labas, Yulii A., ...

Homologs of the green fluorescent protein (GFP), including the recently described GFP-like domains of certain extracellular matrix proteins in Bilaterian organisms, are remarkably similar at the...

Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina (2002)

Rebrikov, Denis V, Bulina, Maria E, Bogdanova, Ekaterina A, Vagner, Loura L, Lukyanov, Sergey A

Abstract Background Freshwater planarians are widely used as models for investigation of pattern formation and studies on genetic variation in populations. Despite extensive information on the...

Identification and characterization of a new family of C-type lectin-like genes from planaria Girardia tigrina (2002)

Shagin, Dmitry A., Barsova, Ekaterina V., Bogdanova, Ekaterina A., Britanova, Olga V., Gurskaya, Nadia G., Lukyanov, Konstantin A., ...

A novel family of C-type lectin–like genes, denoted multidomain free lectin (MDFL), was identified in the freshwater planaria Girardia (Dugesia) tigrina. We cloned several genes that encode...

Color transitions in coral's fluorescent proteins by site-directed mutagenesis (2001)

Gurskaya, Nadya G, Savitsky, Alexander P, Yanushevich, Yurii G, Lukyanov, Sergey A, Lukyanov, Konstantin A

Abstract Background Green Fluorescent Protein (GFP) cloned from jellyfish Aequorea victoria and its homologs from corals Anthozoa have a great practical significance as in vivo markers of gene...

Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization (2000)

Rebrikov, Denis V., Britanova, Olga V., Gurskaya, Nadya G., Lukyanov, Konstantin A., Tarabykin, Victor S., Lukyanov, Sergey A.

Suppression subtractive hybridization (SSH) is one of the most powerful and popular methods for isolating differentially expressed transcripts. However, SSH-generated libraries typically contain some...

PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori

Akopyants, Natalia S., Fradkov, Arkady, Diatchenko, Luda, Hill, Jason E., Siebert, Paul D., Lukyanov, Sergey A., ...

Genes that are characteristic of only certain strains of a bacterial species can be of great biologic interest. Here we describe a PCR-based subtractive hybridization method for efficiently detecting...

Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization

Rebrikov, Denis V., Britanova, Olga V., Gurskaya, Nadya G., Lukyanov, Konstantin A., Tarabykin, Victor S., Lukyanov, Sergey A.

Suppression subtractive hybridization (SSH) is one of the most powerful and popular methods for isolating differentially expressed transcripts. However, SSH-generated libraries typically contain some...

Simple cDNA normalization using kamchatka crab duplex-specific nuclease

Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., ...

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN...

PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori

Akopyants, Natalia S., Fradkov, Arkady, Diatchenko, Luda, Hill, Jason E., Siebert, Paul D., Lukyanov, Sergey A., ...

Genes that are characteristic of only certain strains of a bacterial species can be of great biologic interest. Here we describe a PCR-based subtractive hybridization method for efficiently detecting...

Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization

Rebrikov, Denis V., Britanova, Olga V., Gurskaya, Nadya G., Lukyanov, Konstantin A., Tarabykin, Victor S., Lukyanov, Sergey A.

Suppression subtractive hybridization (SSH) is one of the most powerful and popular methods for isolating differentially expressed transcripts. However, SSH-generated libraries typically contain some...

Simple cDNA normalization using kamchatka crab duplex-specific nuclease

Zhulidov, Pavel A., Bogdanova, Ekaterina A., Shcheglov, Alex S., Vagner, Laura L., Khaspekov, George L., Kozhemyako, Valery B., ...

We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN...