Servaas A. Morré

Multi locus sequence typing of Chlamydiales: clonal groupings within the obligate intracellular bacteria Chlamydia trachomatis (2008)

Pannekoek, Yvonne, Morelli, Giovanna, Kusecek, Barica, Morré, Servaas A, Ossewaarde, Jacobus M, Langerak, Ankie A, ...

Abstract Background The obligate intracellular growing bacterium Chlamydia trachomatis causes diseases like trachoma, urogenital infection and lymphogranuloma venereum with severe morbidity. Several...

Do host genetic traits in the bacterial sensing system play a role in the development of Chlamydia trachomatis-associated tubal pathology in subfertile women? (2006)

Den Hartog, Janneke E, Ouburg, Sander, Land, Jolande A, Lyons, Joseph M, Ito, James I, Peña, A Salvador, ...

Abstract Background In women, Chlamydia (C.) trachomatis upper genital tract infection can cause distal tubal damage and occlusion, increasing the risk of tubal factor subfertility and ectopic...

Efficacy of an Immune Modulator in Experimental Chlamydia trachomatis Infection of the Female Genital Tract (2006)

Joseph M. Lyons, James I. Ito, Servaas A. Morré

Objective. The aim of this study was to determine if vaginal application of the immune response modifier imiquimod (Aldara cream, 3M Pharmaceuticals, St Paul, Minn) would alter the course and/or...

Efficacy of an Immune Modulator in Experimental Chlamydia trachomatis Infection of the Female Genital Tract (2006)

Joseph M. Lyons, James I. Ito, Servaas A. Morré

Objective. The aim of this study was to determine if vaginal application of the immune response modifier imiquimod (Aldara cream, 3M Pharmaceuticals, St Paul, Minn) would alter the course and/or...

The CD14functional gene polymorphism -260 C>T is not involved in either the susceptibility to Chlamydia trachomatisinfection or the development of tubal pathology (2005)

Ouburg, Sander, Spaargaren, Joke, Den Hartog, Janneke E, Land, Jolande A, Fennema, Johan SA, Pleijster, Jolein, ...

Abstract Background The functional polymorphism -260 C>T in the LPS sensing TLR4 co-receptor CD14 gene enhances the transcriptional activity and results in a higher CD14 receptor density. Individuals...

Acquired homotypic and heterotypic immunity against oculogenital Chlamydia trachomatisserovars following female genital tract infection in mice (2005)

Lyons, Joseph M, Morré, Servaas A, Airo-Brown, Lucy P, Peña, A Salvador, Ito, James I

Abstract Background Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen causing female genital tract infection throughout the world. Reinfection with the same serovar, as...

Specific-pathogen-free pigs as an animal model for studying Chlamydia trachomatis genital infection (2005)

Hoang, Thi Q T, De Vos, Liselotte, Verminnen, Kristel, Harkinezhad, Taher, Chiers, Koen, ...

The purpose of the present study was to evaluate pigs as a large-animal model for female genital infection with two Chlamydia trachomatis human serovar E strains. Sixteen-week-old...

Overestimation of complication rates in evaluations of Chlamydia trachomatis screening programmes--implications for cost-effectiveness analyses (2004)

Van Valkengoed, Irene GM, Morré, Servaas A, Van Den Brule, Adriaan JC, Meijer, Chris JLM, Bouter, Lex M, Boeke, A Joan P

Background Cost-effectiveness analyses of screening programmes for asymptomatic Chlamydia trachomatis infection suggest that screening at low prevalences in the population is cost-effective. However,...

Interleukin-1B (IL-1B) and interleukin-1 receptor antagonist (IL-1RN) gene polymorphisms are not associated with tubal pathology and Chlamydia trachomatis-related tubal factor subfertility (2003)

Murillo, Laura S., Land, Jolande A., Pleijster, Jolein, Bruggeman, Cathrien A., Peña, A. Salvador, Morré, Servaas A.

BACKGROUND: Chlamydia trachomatis infections have been associated with tubal pathology. However, not all C.trachomatis‐infected women actually develop tubal pathology. Recently, host genetic...

Determination of Chlamydia trachomatis Prevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens

Morré, Servaas A., Moes, Robert M., Boeke, A. Joan P.

This study determined the performances of the LCx (Abbott) and COBAS Amplicor (Roche) tests with urine specimens for the detection of Chlamydia trachomatis in an asymptomatic screening population....

Pooling of Urine Specimens for Detection of Asymptomatic Chlamydia trachomatis Infections by PCR in a Low-Prevalence Population: Cost-Saving Strategy for Epidemiological Studies and Screening Programs

Morré, Servaas A., Munk, Christian, Krüger-Kjaer, Susanne, Winther, Jeanette F., Jørgensens, Hans O., ...

Pooling, in groups of five, of urine specimens from asymptomatically infected men in a population with 4% prevalence, as determined by case finding, is 100% sensitive and specific and results in a...

Analysis of Genetic Heterogeneity in Chlamydia trachomatis Clinical Isolates of Serovars D, E, and F by Amplified Fragment Length Polymorphism

Morré, Servaas A., Ossewaarde, Jacobus M., Savelkoul, Paul H. M., Stoof, Jeroen

Amplified fragment length polymorphism (AFLP) fingerprinting of clinical isolates of Chlamydia trachomatis serovars D, E, and F showed a low percentage of genetic heterogeneity, but clear differences...

Mailed, Home-Obtained Urine Specimens: a Reliable Screening Approach for Detecting Asymptomatic Chlamydia trachomatis Infections

Morré, Servaas A., De Jong, Afke, Boeke, A. Joan P., ...

The use of mailed, home-obtained urine specimens could facilitate screening programs for the detection of asymptomatic Chlamydia trachomatis infections. Since transport time could have an adverse...

Normal IncA Expression and Fusogenicity of Inclusions in Chlamydia trachomatis Isolates with the incA I47T Mutation

Pannekoek, Yvonne, Van Der Ende, Arie, Eijk, Paul P., Van Marle, Jan, De Witte, Moniek A., Ossewaarde, Jacobus M., ...

To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were...

Genomic Relatedness of Chlamydia Isolates Determined by Amplified Fragment Length Polymorphism Analysis

Meijer, Adam, Morré, Servaas A., Savelkoul, Paul H. M., Ossewaarde, Jacobus M.

The genomic relatedness of 19 Chlamydia pneumoniae isolates (17 from respiratory origin and 2 from atherosclerotic origin), 21 Chlamydia trachomatis isolates (all serovars from the human biovar, an...

Serotyping and Genotyping of Genital Chlamydia trachomatis Isolates Reveal Variants of Serovars Ba, G, and J as Confirmed by omp1 Nucleotide Sequence Analysis

Morré, Servaas A., Ossewaarde, Jacobus M., Lan, Jar, Walboomers, Jan M. M., MacLaren, David M., ...

Urogenital isolates (n = 93) of Chlamydia trachomatis were differentiated into serovars and variants by serotyping with monoclonal antibodies and genotyping by restriction fragment length...

Genotyping of Chlamydia trachomatis in Urine Specimens Will Facilitate Large Epidemiological Studies

Morré, Servaas A., Moes, Robert, Van Valkengoed, Irene, Boeke, Joan P., ...

The serovars of Chlamydia trachomatis-positive urine specimens (n = 81; as detected by PCR and ligase chain reaction) were successfully analyzed in 94% of cases by omp1 PCR-based RFLP analysis. The...

Comparison of Three Commercially Available Peptide-Based Immunoglobulin G (IgG) and IgA Assays to Microimmunofluorescence Assay for Detection of Chlamydia trachomatis Antibodies

Morré, Servaas A., Munk, Christian, Persson, Kenneth, Krüger-Kjaer, Susanne, Van Dijk, Rogier, ...

Three commercially available, peptide-based enzyme-linked immunosorbent assay (ELISA) systems (Chlamydia trachomatis IgG and IgA EIA [CT-EIA; Labsystems OY, Helsinki, Finland], SeroCT IgG and IgA...

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

Molano, Monica, Morré, Servaas A., Pol, Rene

In this study we developed and evaluated a new PCR-based typing assay, directed to the VD2 region of the omp1 gene, for the detection and typing of urogenital Chlamydia trachomatis infections. A...

Interrelationship between Polymorphisms of incA, Fusogenic Properties of Chlamydia trachomatis Strains, and Clinical Manifestations in Patients in The Netherlands

Pannekoek, Yvonne, Spaargaren, Joke, Langerak, Ankie A. J., Merks, Judith, Morré, Servaas A., Van Der Ende, Arie

IncA variation among Dutch Chlamydia trachomatis isolates was investigated. Of 98 strains, two carried an incA with a premature stop codon, lacked IncA, and were nonfusogenic, while 96 contained an...

Specific-Pathogen-Free Pigs as an Animal Model for Studying Chlamydia trachomatis Genital Infection

Vanrompay, Daisy, Hoang, Thi Q. T., De Vos, Liselotte, Verminnen, Kristel, Harkinezhad, Taher, Chiers, Koen, ...

The purpose of the present study was to evaluate pigs as a large-animal model for female genital infection with two Chlamydia trachomatis human serovar E strains. Sixteen-week-old...

Follow-up, treatment, and reinfection rates among asymptomatic chlamydia trachomatis cases in general practice.

Morré, Servaas A, Bouter, Lex M, ...

BACKGROUND: Adequate treatment and follow-up of patients is essential to the success of a screening programme for Chlamydia trachomatis. There has been a lack of data on follow-up, confirmation of...

Partner notification among asymptomatic Chlamydia trachomatis cases, by means of mailed specimens.

Morré, Servaas A, Bouter, Lex M, ...

The objective of the study was to evaluate the prevalence of Chlamydia trachomatis infection and the participation, among partners of asymptomatic cases in general practice. Index cases were...

Efficacy of an Immune Modulator in Experimental Chlamydia trachomatis Infection of the Female Genital Tract

Lyons, Joseph M., Ito, James I., Morré, Servaas A.

Objective. The aim of this study was to determine if vaginal application of the immune response modifier imiquimod (Aldara cream, 3M Pharmaceuticals, St Paul, Minn) would alter the course and/or...

Determination of Chlamydia trachomatis Prevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens

Morré, Servaas A., Moes, Robert M., Boeke, A. Joan P.

This study determined the performances of the LCx (Abbott) and COBAS Amplicor (Roche) tests with urine specimens for the detection of Chlamydia trachomatis in an asymptomatic screening population....

Pooling of Urine Specimens for Detection of Asymptomatic Chlamydia trachomatis Infections by PCR in a Low-Prevalence Population: Cost-Saving Strategy for Epidemiological Studies and Screening Programs

Morré, Servaas A., Munk, Christian, Krüger-Kjaer, Susanne, Winther, Jeanette F., Jørgensens, Hans O., ...

Pooling, in groups of five, of urine specimens from asymptomatically infected men in a population with 4% prevalence, as determined by case finding, is 100% sensitive and specific and results in a...

Analysis of Genetic Heterogeneity in Chlamydia trachomatis Clinical Isolates of Serovars D, E, and F by Amplified Fragment Length Polymorphism

Morré, Servaas A., Ossewaarde, Jacobus M., Savelkoul, Paul H. M., Stoof, Jeroen

Amplified fragment length polymorphism (AFLP) fingerprinting of clinical isolates of Chlamydia trachomatis serovars D, E, and F showed a low percentage of genetic heterogeneity, but clear differences...

Mailed, Home-Obtained Urine Specimens: a Reliable Screening Approach for Detecting Asymptomatic Chlamydia trachomatis Infections

Morré, Servaas A., De Jong, Afke, Boeke, A. Joan P., ...

The use of mailed, home-obtained urine specimens could facilitate screening programs for the detection of asymptomatic Chlamydia trachomatis infections. Since transport time could have an adverse...

Normal IncA Expression and Fusogenicity of Inclusions in Chlamydia trachomatis Isolates with the incA I47T Mutation

Pannekoek, Yvonne, Van Der Ende, Arie, Eijk, Paul P., Van Marle, Jan, De Witte, Moniek A., Ossewaarde, Jacobus M., ...

To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were...

Genomic Relatedness of Chlamydia Isolates Determined by Amplified Fragment Length Polymorphism Analysis

Meijer, Adam, Morré, Servaas A., Savelkoul, Paul H. M., Ossewaarde, Jacobus M.

The genomic relatedness of 19 Chlamydia pneumoniae isolates (17 from respiratory origin and 2 from atherosclerotic origin), 21 Chlamydia trachomatis isolates (all serovars from the human biovar, an...

Serotyping and Genotyping of Genital Chlamydia trachomatis Isolates Reveal Variants of Serovars Ba, G, and J as Confirmed by omp1 Nucleotide Sequence Analysis

Morré, Servaas A., Ossewaarde, Jacobus M., Lan, Jar, Walboomers, Jan M. M., MacLaren, David M., ...

Urogenital isolates (n = 93) of Chlamydia trachomatis were differentiated into serovars and variants by serotyping with monoclonal antibodies and genotyping by restriction fragment length...

Genotyping of Chlamydia trachomatis in Urine Specimens Will Facilitate Large Epidemiological Studies

Morré, Servaas A., Moes, Robert, Van Valkengoed, Irene, Boeke, Joan P., ...

The serovars of Chlamydia trachomatis-positive urine specimens (n = 81; as detected by PCR and ligase chain reaction) were successfully analyzed in 94% of cases by omp1 PCR-based RFLP analysis. The...

Comparison of Three Commercially Available Peptide-Based Immunoglobulin G (IgG) and IgA Assays to Microimmunofluorescence Assay for Detection of Chlamydia trachomatis Antibodies

Morré, Servaas A., Munk, Christian, Persson, Kenneth, Krüger-Kjaer, Susanne, Van Dijk, Rogier, ...

Three commercially available, peptide-based enzyme-linked immunosorbent assay (ELISA) systems (Chlamydia trachomatis IgG and IgA EIA [CT-EIA; Labsystems OY, Helsinki, Finland], SeroCT IgG and IgA...

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

Molano, Monica, Morré, Servaas A., Pol, Rene

In this study we developed and evaluated a new PCR-based typing assay, directed to the VD2 region of the omp1 gene, for the detection and typing of urogenital Chlamydia trachomatis infections. A...

Interrelationship between Polymorphisms of incA, Fusogenic Properties of Chlamydia trachomatis Strains, and Clinical Manifestations in Patients in The Netherlands

Pannekoek, Yvonne, Spaargaren, Joke, Langerak, Ankie A. J., Merks, Judith, Morré, Servaas A., Van Der Ende, Arie

IncA variation among Dutch Chlamydia trachomatis isolates was investigated. Of 98 strains, two carried an incA with a premature stop codon, lacked IncA, and were nonfusogenic, while 96 contained an...

Specific-Pathogen-Free Pigs as an Animal Model for Studying Chlamydia trachomatis Genital Infection

Vanrompay, Daisy, Hoang, Thi Q. T., De Vos, Liselotte, Verminnen, Kristel, Harkinezhad, Taher, Chiers, Koen, ...

The purpose of the present study was to evaluate pigs as a large-animal model for female genital infection with two Chlamydia trachomatis human serovar E strains. Sixteen-week-old...

Follow-up, treatment, and reinfection rates among asymptomatic chlamydia trachomatis cases in general practice.

Morré, Servaas A, Bouter, Lex M, ...

BACKGROUND: Adequate treatment and follow-up of patients is essential to the success of a screening programme for Chlamydia trachomatis. There has been a lack of data on follow-up, confirmation of...

Partner notification among asymptomatic Chlamydia trachomatis cases, by means of mailed specimens.

Morré, Servaas A, Bouter, Lex M, ...

The objective of the study was to evaluate the prevalence of Chlamydia trachomatis infection and the participation, among partners of asymptomatic cases in general practice. Index cases were...

Efficacy of an Immune Modulator in Experimental Chlamydia trachomatis Infection of the Female Genital Tract

Lyons, Joseph M., Ito, James I., Morré, Servaas A.

Objective. The aim of this study was to determine if vaginal application of the immune response modifier imiquimod (Aldara cream, 3M Pharmaceuticals, St Paul, Minn) would alter the course and/or...