Sharon J. Peacock

Burkholderia pseudomalleigenome plasticity associated with genomic island variation (2008)

Tumapa, Sarinna, Holden, Matthew TG, Vesaratchavest, Mongkol, Wuthiekanun, Vanaporn, Limmathurotsakul, Direk, Chierakul, Wirongrong, ...

Abstract Background Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene transfer contributes to the genetic diversity of this pathogen and may be an...

Toll-Like Receptor 2 Impairs Host Defense in Gram-Negative Sepsis Caused by Burkholderia pseudomallei (Melioidosis) (2007)

W. Joost Wiersinga, Catharina W. Wieland, Mark C. Dessing, Narisara Chantratita, Allen C. Cheng, Direk Limmathurotsakul, ...

BackgroundToll-like receptors (TLRs) are essential in host defense against pathogens by virtue of their capacity to detect microbes and initiate the immune response. TLR2 is seen as the most...

BurkholderiaHep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis (2007)

Tiyawisutsri, Rachaneeporn, Holden, Matthew TG, Tumapa, Sarinna, Rengpipat, Sirirat, Clarke, Simon R, Foster, Simon J, ...

Abstract Background The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have...

BurkholderiaHep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis (2007)

Tiyawisutsri, Rachaneeporn, Holden, Matthew TG, Tumapa, Sarinna, Rengpipat, Sirirat, Clarke, Simon R, Foster, Simon J, ...

Abstract Background The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have...

Multilocus Sequence Typing for Characterization of Methicillin-Resistant and Methicillin-Susceptible Clones of Staphylococcus aureus

Enright, Mark C., Day, Nicholas P. J., Davies, Catrin E., Peacock, Sharon J., Spratt, Brian G.

A multilocus sequence typing (MLST) scheme has been developed for Staphylococcus aureus. The sequences of internal fragments of seven housekeeping genes were obtained for 155 S. aureus isolates from...

Virulent Combinations of Adhesin and Toxin Genes in Natural Populations of Staphylococcus aureus

Peacock, Sharon J., Moore, Catrin E., Justice, Anita, Kantzanou, Maria, Story, Lisa, Mackie, Kathryn, ...

Most cases of severe Staphylococcus aureus disease cannot be explained by the action of a single virulence determinant, and it is likely that a number of factors act in combination during the...

Functional Blocking of Staphylococcus aureus Adhesins following Growth in Ex Vivo Media

Massey, Ruth C., Dissanayeke, Shobana R., Cameron, Brian, Ferguson, David, Foster, Timothy J., Peacock, Sharon J.

Defining the role of Staphylococcus aureus adhesins in disease pathogenesis may depend on the use of bacteria grown in culture media that more closely reflect the human milieu than conventional...

Determinants of Acquisition and Carriage of Staphylococcus aureus in Infancy

Peacock, Sharon J., Justice, Anita, Griffiths, D., Kantzanou, M. N., Crook, Derrick, ...

Nasal carriage of Staphylococcus aureus is a major risk factor for invasive S. aureus disease. The aim of this study was to define factors associated with carriage. We conducted a prospective,...

Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the rapid evolution of virulence and drug resistance

Holden, Matthew T. G., Feil, Edward J., Lindsay, Jodi A., Peacock, Sharon J., Day, Nicholas P. J., Enright, Mark C., ...

Staphylococcus aureus is an important nosocomial and community-acquired pathogen. Its genetic plasticity has facilitated the evolution of many virulent and drug-resistant strains, presenting a major...

Evaluation of Immunoglobulin M (IgM) and IgG Rapid Cassette Test Kits for Diagnosis of Melioidosis in an Area of Endemicity

Wuthiekanun, Vanaporn, Amornchai, Premjit, Chierakul, Wirongrong, Cheng, Allen C., White, Nicholas J., Peacock, Sharon J., ...

An enzyme-linked immunosorbent assay-based rapid cassette immunoglobulin G (IgG) and IgM immunochromogenic test kit was compared to the indirect hemagglutination test (IHA) for the diagnosis of acute...

Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei

Holden, Matthew T. G., Titball, Richard W., Peacock, Sharon J., Cerdeño-Tárraga, Ana M., Atkins, Timothy, Crossman, Lisa C., ...

Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world...

Detection of Burkholderia pseudomallei in Soil within the Lao People's Democratic Republic

Wuthiekanun, Vanaporn, Mayxay, Mayfong, Chierakul, Wirongrong, Phetsouvanh, Rattanaphone, Cheng, Allen C., White, Nicholas J, ...

Clinical cases of melioidosis caused by the saprophyte Burkholderia pseudomallei were first noted in the Lao People's Democratic Republic (PDR) in 1999. In this study, 36% of 110 soil samples in...

Rapid Immunofluorescence Microscopy for Diagnosis of Melioidosis

Wuthiekanun, Vanaporn, Desakorn, Varunee, Wongsuvan, Gumphol, Amornchai, Premjit, Cheng, Allen C., Maharjan, Bina, ...

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were...

Role and Significance of Quantitative Urine Cultures in Diagnosis of Melioidosis

Limmathurotsakul, Direk, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Cheng, Allen C., Maharjan, Bina, Chaowagul, Wipada, ...

Melioidosis is associated with significant mortality in countries in which it is endemic. Previous studies have demonstrated that quantitative Burkholderia pseudomallei counts in blood are predictive...

Antibodies from Patients with Melioidosis Recognize Burkholderia mallei but Not Burkholderia thailandensis Antigens in the Indirect Hemagglutination Assay

Tiyawisutsri, Rachaneeporn, Peacock, Sharon J., Langa, Sayan, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, ...

The indirect hemagglutination assay routinely used to detect antibodies to Burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to B. pseudomallei, B. mallei, and B....

Comparison of Ashdown's Medium, Burkholderia cepacia Medium, and Burkholderia pseudomallei Selective Agar for Clinical Isolation of Burkholderia pseudomallei

Peacock, Sharon J., Chieng, Grace, Cheng, Allen C., Dance, David A. B., Amornchai, Premjit, Wongsuvan, Gumphol, ...

Ashdown's medium, Burkholderia pseudomallei selective agar (BPSA), and a commercial Burkholderia cepacia medium were compared for their abilities to grow B. pseudomallei from 155 clinical specimens...

Open-Label Randomized Trial of Oral Trimethoprim-Sulfamethoxazole, Doxycycline, and Chloramphenicol Compared with Trimethoprim-Sulfamethoxazole and Doxycycline for Maintenance Therapy of Melioidosis

Chaowagul, Wipada, Chierakul, Wirongrong, Simpson, Andrew J., Short, Jennifer M., Stepniewska, Kasia, Maharjan, Bina, ...

Melioidosis (infection caused by Burkholderia pseudomallei) requires a prolonged course of oral antibiotics following initial intravenous therapy to reduce the risk of relapse after cessation of...

Recurrent Melioidosis in Patients in Northeast Thailand Is Frequently Due to Reinfection Rather than Relapse

Maharjan, Bina, Chantratita, Narisara, Vesaratchavest, Mongkol, Cheng, Allen, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, ...

Human melioidosis is associated with a high rate of recurrent disease, despite adequate antimicrobial treatment. Here, we define the rate of relapse versus the rate of reinfection in 116 patients...

Microarrays Reveal that Each of the Ten Dominant Lineages of Staphylococcus aureus Has a Unique Combination of Surface-Associated and Regulatory Genes†

Lindsay, Jodi A., Moore, Catrin E., Day, Nicholas P., Peacock, Sharon J., Witney, Adam A., Stabler, Richard A., ...

Staphylococcus aureus is the most common cause of hospital-acquired infection. In healthy hosts outside of the health care setting, S. aureus is a frequent colonizer of the human nose but rarely...

Serological Evidence for Increased Human Exposure to Burkholderia pseudomallei following the Tsunami in Southern Thailand

Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Rattanalertnavee, Jurairat, Langa, Sayan, Sirodom, Damrong, Wattanawaitunechai, Charnkij, ...

A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December...

How Clonal Is Staphylococcus aureus?

Feil, Edward J., Cooper, Jessica E., Grundmann, Hajo, Robinson, D. Ashley, Enright, Mark C., Berendt, Tony, ...

Staphylococcus aureus is an important human pathogen and represents a growing public health burden owing to the emergence and spread of antibiotic-resistant clones, particularly within the hospital...

Nonrandom Distribution of Burkholderia pseudomallei Clones in Relation to Geographical Location and Virulence

Vesaratchavest, Mongkol, Tumapa, Sarinna, Day, Nicholas P. J., Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Holden, Matthew T. G., ...

Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern...

Multilocus Sequence Typing for Characterization of Methicillin-Resistant and Methicillin-Susceptible Clones of Staphylococcus aureus

Enright, Mark C., Day, Nicholas P. J., Davies, Catrin E., Peacock, Sharon J., Spratt, Brian G.

A multilocus sequence typing (MLST) scheme has been developed for Staphylococcus aureus. The sequences of internal fragments of seven housekeeping genes were obtained for 155 S. aureus isolates from...

Virulent Combinations of Adhesin and Toxin Genes in Natural Populations of Staphylococcus aureus

Peacock, Sharon J., Moore, Catrin E., Justice, Anita, Kantzanou, Maria, Story, Lisa, Mackie, Kathryn, ...

Most cases of severe Staphylococcus aureus disease cannot be explained by the action of a single virulence determinant, and it is likely that a number of factors act in combination during the...

Functional Blocking of Staphylococcus aureus Adhesins following Growth in Ex Vivo Media

Massey, Ruth C., Dissanayeke, Shobana R., Cameron, Brian, Ferguson, David, Foster, Timothy J., Peacock, Sharon J.

Defining the role of Staphylococcus aureus adhesins in disease pathogenesis may depend on the use of bacteria grown in culture media that more closely reflect the human milieu than conventional...

How Clonal Is Staphylococcus aureus?

Feil, Edward J., Cooper, Jessica E., Grundmann, Hajo, Robinson, D. Ashley, Enright, Mark C., Berendt, Tony, ...

Staphylococcus aureus is an important human pathogen and represents a growing public health burden owing to the emergence and spread of antibiotic-resistant clones, particularly within the hospital...

Determinants of Acquisition and Carriage of Staphylococcus aureus in Infancy

Peacock, Sharon J., Justice, Anita, Griffiths, D., Kantzanou, M. N., Crook, Derrick, ...

Nasal carriage of Staphylococcus aureus is a major risk factor for invasive S. aureus disease. The aim of this study was to define factors associated with carriage. We conducted a prospective,...

Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the rapid evolution of virulence and drug resistance

Holden, Matthew T. G., Feil, Edward J., Lindsay, Jodi A., Peacock, Sharon J., Day, Nicholas P. J., Enright, Mark C., ...

Staphylococcus aureus is an important nosocomial and community-acquired pathogen. Its genetic plasticity has facilitated the evolution of many virulent and drug-resistant strains, presenting a major...

Evaluation of Immunoglobulin M (IgM) and IgG Rapid Cassette Test Kits for Diagnosis of Melioidosis in an Area of Endemicity

Wuthiekanun, Vanaporn, Amornchai, Premjit, Chierakul, Wirongrong, Cheng, Allen C., White, Nicholas J., Peacock, Sharon J., ...

An enzyme-linked immunosorbent assay-based rapid cassette immunoglobulin G (IgG) and IgM immunochromogenic test kit was compared to the indirect hemagglutination test (IHA) for the diagnosis of acute...

Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei

Holden, Matthew T. G., Titball, Richard W., Peacock, Sharon J., Cerdeño-Tárraga, Ana M., Atkins, Timothy, Crossman, Lisa C., ...

Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world...

Detection of Burkholderia pseudomallei in Soil within the Lao People's Democratic Republic

Wuthiekanun, Vanaporn, Mayxay, Mayfong, Chierakul, Wirongrong, Phetsouvanh, Rattanaphone, Cheng, Allen C., White, Nicholas J, ...

Clinical cases of melioidosis caused by the saprophyte Burkholderia pseudomallei were first noted in the Lao People's Democratic Republic (PDR) in 1999. In this study, 36% of 110 soil samples in...

Rapid Immunofluorescence Microscopy for Diagnosis of Melioidosis

Wuthiekanun, Vanaporn, Desakorn, Varunee, Wongsuvan, Gumphol, Amornchai, Premjit, Cheng, Allen C., Maharjan, Bina, ...

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were...

Role and Significance of Quantitative Urine Cultures in Diagnosis of Melioidosis

Limmathurotsakul, Direk, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Cheng, Allen C., Maharjan, Bina, Chaowagul, Wipada, ...

Melioidosis is associated with significant mortality in countries in which it is endemic. Previous studies have demonstrated that quantitative Burkholderia pseudomallei counts in blood are predictive...

Antibodies from Patients with Melioidosis Recognize Burkholderia mallei but Not Burkholderia thailandensis Antigens in the Indirect Hemagglutination Assay

Tiyawisutsri, Rachaneeporn, Peacock, Sharon J., Langa, Sayan, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, ...

The indirect hemagglutination assay routinely used to detect antibodies to Burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to B. pseudomallei, B. mallei, and B....

Comparison of Ashdown's Medium, Burkholderia cepacia Medium, and Burkholderia pseudomallei Selective Agar for Clinical Isolation of Burkholderia pseudomallei

Peacock, Sharon J., Chieng, Grace, Cheng, Allen C., Dance, David A. B., Amornchai, Premjit, Wongsuvan, Gumphol, ...

Ashdown's medium, Burkholderia pseudomallei selective agar (BPSA), and a commercial Burkholderia cepacia medium were compared for their abilities to grow B. pseudomallei from 155 clinical specimens...

Open-Label Randomized Trial of Oral Trimethoprim-Sulfamethoxazole, Doxycycline, and Chloramphenicol Compared with Trimethoprim-Sulfamethoxazole and Doxycycline for Maintenance Therapy of Melioidosis

Chaowagul, Wipada, Chierakul, Wirongrong, Simpson, Andrew J., Short, Jennifer M., Stepniewska, Kasia, Maharjan, Bina, ...

Melioidosis (infection caused by Burkholderia pseudomallei) requires a prolonged course of oral antibiotics following initial intravenous therapy to reduce the risk of relapse after cessation of...

Recurrent Melioidosis in Patients in Northeast Thailand Is Frequently Due to Reinfection Rather than Relapse

Maharjan, Bina, Chantratita, Narisara, Vesaratchavest, Mongkol, Cheng, Allen, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, ...

Human melioidosis is associated with a high rate of recurrent disease, despite adequate antimicrobial treatment. Here, we define the rate of relapse versus the rate of reinfection in 116 patients...

Microarrays Reveal that Each of the Ten Dominant Lineages of Staphylococcus aureus Has a Unique Combination of Surface-Associated and Regulatory Genes†

Lindsay, Jodi A., Moore, Catrin E., Day, Nicholas P., Peacock, Sharon J., Witney, Adam A., Stabler, Richard A., ...

Staphylococcus aureus is the most common cause of hospital-acquired infection. In healthy hosts outside of the health care setting, S. aureus is a frequent colonizer of the human nose but rarely...

Serological Evidence for Increased Human Exposure to Burkholderia pseudomallei following the Tsunami in Southern Thailand

Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Rattanalertnavee, Jurairat, Langa, Sayan, Sirodom, Damrong, Wattanawaitunechai, Charnkij, ...

A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December...

Nonrandom Distribution of Burkholderia pseudomallei Clones in Relation to Geographical Location and Virulence

Vesaratchavest, Mongkol, Tumapa, Sarinna, Day, Nicholas P. J., Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Holden, Matthew T. G., ...

Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern...

Accuracy of Enzyme-Linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis▿

Chantratita, Narisara, Wuthiekanun, Vanaporn, Thanwisai, Aunchalee, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, ...

Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast...

Biological Relevance of Colony Morphology and Phenotypic Switching by Burkholderia pseudomallei▿

Chantratita, Narisara, Wuthiekanun, Vanaporn, Boonbumrung, Khaemaporn, Tiyawisutsri, Rachaneeporn, Vesaratchavest, Mongkol, Limmathurotsakul, Direk, ...

Melioidosis is a notoriously protracted illness and is difficult to cure. We hypothesize that the causative organism, Burkholderia pseudomallei, undergoes a process of adaptation involving altered...

Improved Multilocus Sequence Typing Scheme for Staphylococcus epidermidis▿

Thomas, Jonathan C., Vargas, Miguel R., Miragaia, Maria, Peacock, Sharon J., Archer, Gordon L., Enright, Mark C.

We evaluated three multilocus sequence typing (MLST) schemes for Staphylococcus epidermidis and selected the seven most discriminatory loci for the formation of a new, more powerful MLST scheme. This...

Optimization of Culture of Leptospira from Humans with Leptospirosis▿

Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Limmathurotsakul, Direk, Smythe, Lee D., Symonds, Meegan L., Dohnt, Michael F., ...

A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison...

Toll-Like Receptor 2 Impairs Host Defense in Gram-Negative Sepsis Caused by Burkholderia pseudomallei (Melioidosis)

Wiersinga, W. Joost, Wieland, Catharina W, Dessing, Mark C, Chantratita, Narisara, Cheng, Allen C, Limmathurotsakul, Direk, ...

Willem Wiersinga and colleagues find up-regulation of multiple Toll-like receptors (TLRs) in peripheral blood cells of patients with melioidosis. However, only TLR2 had an effect on the immune...

High-Throughput mRNA Profiling Characterizes the Expression of Inflammatory Molecules in Sepsis Caused by Burkholderia pseudomallei▿

Wiersinga, W. Joost, Dessing, Mark C., Kager, Piet A., Cheng, Allen C., Limmathurotsakul, Direk, Day, Nicholas P., ...

Sepsis is characterized by an uncontrolled inflammatory response to invading microorganisms. We describe the inflammatory mRNA profiles in whole-blood leukocytes, monocytes, and granulocytes using a...

A Dominant Clone of Leptospira interrogans Associated with an Outbreak of Human Leptospirosis in Thailand

Thaipadungpanit, Janjira, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Smythe, Lee D., Petkanchanapong, Wimol, Limpaiboon, Roongrueng, ...

A sustained outbreak of human leptospirosis occurred in northeast Thailand between 1999 and 2003, the basis for which was unknown. Leptospirosis is a potentially serious infection cause by bacteria...

Endogenous Interleukin-18 Improves the Early Antimicrobial Host Response in Severe Melioidosis▿

Wiersinga, W. Joost, Wieland, Catharina W., De Boer, Anita, Florquin, Sandrine, Dondorp, Arjen, ...

Melioidosis is caused by the soil saprophyte Burkholderia pseudomallei and is endemic in Southeast Asia. The pathogenesis of melioidosis is still largely unknown, although gamma interferon (IFN-γ)...

Doxycycline versus Azithromycin for Treatment of Leptospirosis and Scrub Typhus▿

Phimda, Kriangsak, Hoontrakul, Siriwan, Suttinont, Chuanpit, Chareonwat, Sompong, Losuwanaluk, Kitti, Chueasuwanchai, Sunee, ...

Leptospirosis and scrub typhus are important causes of acute fever in Southeast Asia. Options for empirical therapy include doxycycline and azithromycin, but it is unclear whether their efficacies...