Hiroyuki Nakai, Xiaolin Wu, Sally Fuess, Theresa A. Storm, David Munroe, Eugenio Montini, ...
Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low frequency of chromosomal integration of vector genomes, recent studies have raised concerns about the...
Zeng, Jue, Yan, Jizhou, Wang, Ting, Mosbrook-Davis, Deborah, Dolan, Kyle T., Christensen, Ryan, ...
Knowledge of all binding sites for transcriptional activators and repressors is essential for computationally aided identification of transcriptional networks. The techniques developed for defining...
Using retroviruses as a mutagenesis tool to explore the zebrafish genome (2008)
Jao, Li-En, Maddison, Lisette, Chen, Wenbiao, Burgess, Shawn M.
We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen...
High-Resolution Genome-Wide Mapping of Transposon Integration in Mammals (2004)
Stephen R. Yant, Xiaolin Wu, Yong Huang, Brian Garrison, Shawn M. Burgess, Mark A. Kay
The Sleeping Beauty (SB) transposon is an emerging tool for transgenesis, gene discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions in primary and cultured...
Transgenic zebrafish produced by retroviral infection of in vitro-cultured sperm
Kurita, Kayoko, Burgess, Shawn M., Sakai, Noriyoshi
Transgenic modification of sperm before fertilization has distinct advantages over conventional transgenic methods. The primary advantage is that the mosaicism inherent in those other techniques is...
High-Resolution Genome-Wide Mapping of Transposon Integration in Mammals
Yant, Stephen R., Wu, Xiaolin, Huang, Yong, Garrison, Brian, Burgess, Shawn M., Kay, Mark A.
The Sleeping Beauty (SB) transposon is an emerging tool for transgenesis, gene discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions in primary and cultured...
Wu, Xiaolin, Li, Yuan, Crise, Bruce, Burgess, Shawn M., Munroe, David J.
Integration into the host genome is one of the hallmarks of the retroviral life cycle and is catalyzed by virus-encoded integrases. While integrase has strict sequence requirements for the viral DNA...
Large-Scale Molecular Characterization of Adeno-Associated Virus Vector Integration in Mouse Liver
Nakai, Hiroyuki, Wu, Xiaolin, Fuess, Sally, Storm, Theresa A., Munroe, David, Montini, Eugenio, ...
Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low frequency of chromosomal integration of vector genomes, recent studies have raised concerns about the...
Yan, Jizhou, Xu, Lisha, Crawford, Gregory, Wang, Zenfeng, Burgess, Shawn M.
All forkhead (Fox) proteins contain a highly conserved DNA binding domain whose structure is remarkably similar to the winged-helix structures of histones H1 and H5. Little is known about Fox protein...
Transgenic zebrafish produced by retroviral infection of in vitro-cultured sperm
Kurita, Kayoko, Burgess, Shawn M., Sakai, Noriyoshi
Transgenic modification of sperm before fertilization has distinct advantages over conventional transgenic methods. The primary advantage is that the mosaicism inherent in those other techniques is...
High-Resolution Genome-Wide Mapping of Transposon Integration in Mammals
Yant, Stephen R., Wu, Xiaolin, Huang, Yong, Garrison, Brian, Burgess, Shawn M., Kay, Mark A.
The Sleeping Beauty (SB) transposon is an emerging tool for transgenesis, gene discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions in primary and cultured...
Wu, Xiaolin, Li, Yuan, Crise, Bruce, Burgess, Shawn M., Munroe, David J.
Integration into the host genome is one of the hallmarks of the retroviral life cycle and is catalyzed by virus-encoded integrases. While integrase has strict sequence requirements for the viral DNA...
Large-Scale Molecular Characterization of Adeno-Associated Virus Vector Integration in Mouse Liver
Nakai, Hiroyuki, Wu, Xiaolin, Fuess, Sally, Storm, Theresa A., Munroe, David, Montini, Eugenio, ...
Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low frequency of chromosomal integration of vector genomes, recent studies have raised concerns about the...
Yan, Jizhou, Xu, Lisha, Crawford, Gregory, Wang, Zenfeng, Burgess, Shawn M.
All forkhead (Fox) proteins contain a highly conserved DNA binding domain whose structure is remarkably similar to the winged-helix structures of histones H1 and H5. Little is known about Fox protein...
Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions
Wang, Dongmei, Jao, Li-En, Zheng, Naizhong, Dolan, Kyle, Ivey, Jessica, Zonies, Seth, ...
Using a combination of techniques we developed, we infected zebrafish embryos using pseudotyped retroviruses and mapped the genomic locations of the proviral integrations in the F1 offspring of the...
Zeng, Jue, Yan, Jizhou, Wang, Ting, Mosbrook-Davis, Deborah, Dolan, Kyle T., Christensen, Ryan, ...
Knowledge of all binding sites for transcriptional activators and repressors is essential for computationally aided identification of transcriptional networks. The techniques developed for defining...
Youngman, Matthew J., Hobbs, Alyson E. Aiken, Burgess, Shawn M., Srinivasan, Maithreyan, Jensen, Robert E.
The mitochondrial outer membrane protein, Mmm1p, is required for normal mitochondrial shape in yeast. To identify new morphology proteins, we isolated mutations incompatible with the mmm1-1 mutant....
Wang, Ting, Zeng, Jue, Lowe, Craig B., Sellers, Robert G., Salama, Sofie R., Yang, Min, ...
The evolutionary forces that establish and hone target gene networks of transcription factors are largely unknown. Transposition of retroelements may play a role, but its global importance, beyond a...
Phoenix Is Required for Mechanosensory Hair Cell Regeneration in the Zebrafish Lateral Line
Behra, Martine, Bradsher, John, Sougrat, Rachid, Gallardo, Viviana, Allende, Miguel L., Burgess, Shawn M.
In humans, the absence or irreversible loss of hair cells, the sensory mechanoreceptors in the cochlea, accounts for a large majority of acquired and congenital hearing disorders. In the auditory and...
Using retroviruses as a mutagenesis tool to explore the zebrafish genome
Jao, Li-En, Maddison, Lisette, Chen, Wenbiao, Burgess, Shawn M.
We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen...