Shelley Sazer

Disruption of the YRB2 Gene Retards Nuclear Protein Export, Causing a Profound Mitotic Delay, and Can Be Rescued by Overexpression of XPO1/CRM1 (1999)

Noguchi, Eishi, Saitoh, Yoh-hei, Sazer, Shelley, Nishimoto, Takeharu

Disruption of the YRB2 gene encoding a nuclear Ran-binding protein homologous to Yrb1p/RanBP1 makes Saccharomyces cerevisiae cold sensitive for colony-formation, but not for growth in liquid...

The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex

He, Xiangwei, Patterson, Thomas E., Sazer, Shelley

The spindle checkpoint monitors mitotic spindle integrity and the attachment of kinetochores to the spindle. Upon sensing a defect the checkpoint blocks cell cycle progression and thereby prevents...

Effects of Genome Position and the DNA Damage Checkpoint on the Structure and Frequency of sod2 Gene Amplification in Fission Yeast

Patterson, Thomas E., Albrecht, Elizabeth B., Nurse, Paul, Sazer, Shelley, Stark, George R.

The Schizosaccharomyces pombe sod2 gene, located near the telomere on the long arm of chromosome I, encodes a Na+ (or Li+)/H+ antiporter. Amplification of sod2 has previously been shown to confer...

Redundant control of rereplication in fission yeast

Gopalakrishnan, Vidya, Simancek, Pamela, Houchens, Christopher, Snaith, Hilary A., Frattini, Mark G., Sazer, Shelley, ...

The initiation of DNA replication at replication origins in eukaryotic cells is tightly controlled to ensure that the genome is duplicated only once each cell cycle. We present evidence that in...

The Role of fnx1, a Fission Yeast Multidrug Resistance Protein, in the Transition of Cells to a Quiescent G0 State

Dimitrov, Krassen, Sazer, Shelley

Most microorganisms live in conditions of nutrient limitation in their natural habitats. When exposed to these conditions they respond with physiological and morphological changes that enable them to...

The Ran GTPase System in Fission Yeast Affects Microtubules and Cytokinesis in Cells That Are Competent for Nucleocytoplasmic Protein Transport

Salus, Sandra S., Demeter, Janos, Sazer, Shelley

Misregulation of the evolutionarily conserved GTPase Ran in fission yeast results in defects in several cellular processes in cells that are competent for nucleocytoplasmic protein transport. These...

HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes

Chang, Fred, Re, Fabio, Sebastian, Sarah, Sazer, Shelley, Luban, Jeremy

Human immunodeficiency virus type 1 (HIV-1) Vpr is a 15-kDa accessory protein that contributes to several steps in the viral replication cycle and promotes virus-associated pathology. Previous...

The Fission Yeast Nup107-120 Complex Functionally Interacts with the Small GTPase Ran/Spi1 and Is Required for mRNA Export, Nuclear Pore Distribution, and Proper Cell Division

Baï, Siau Wei, Rouquette, Jacques, Umeda, Makoto, Faigle, Wolfgang, Loew, Damarys, Sazer, Shelley, ...

We have characterized Schizosaccharomyces pombe open reading frames encoding potential orthologues of constituents of the evolutionarily conserved Saccharomyces cerevisiae Nup84 vertebrate Nup107-160...

The A78V Mutation in the Mad3-like Domain of Schizosaccharomyces pombe Bub1p Perturbs Nuclear Accumulation and Kinetochore Targeting of Bub1p, Bub3p, and Mad3p and Spindle Assembly Checkpoint Function

Kadura, Sheila, He, Xiangwei, Vanoosthuyse, Vincent, Hardwick, Kevin G., Sazer, Shelley

During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby...

The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression

Umeda, Makoto, Izaddoost, Shahed, Cushman, Ian, Moore, Mary Shannon, Sazer, Shelley

The nuclear import of classical nuclear localization signal-containing proteins depends on importin-α transport receptors. In budding yeast there is a single importin-α gene and in higher...

The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex

He, Xiangwei, Patterson, Thomas E., Sazer, Shelley

The spindle checkpoint monitors mitotic spindle integrity and the attachment of kinetochores to the spindle. Upon sensing a defect the checkpoint blocks cell cycle progression and thereby prevents...

Effects of Genome Position and the DNA Damage Checkpoint on the Structure and Frequency of sod2 Gene Amplification in Fission Yeast

Patterson, Thomas E., Albrecht, Elizabeth B., Nurse, Paul, Sazer, Shelley, Stark, George R.

The Schizosaccharomyces pombe sod2 gene, located near the telomere on the long arm of chromosome I, encodes a Na+ (or Li+)/H+ antiporter. Amplification of sod2 has previously been shown to confer...

Redundant control of rereplication in fission yeast

Gopalakrishnan, Vidya, Simancek, Pamela, Houchens, Christopher, Snaith, Hilary A., Frattini, Mark G., Sazer, Shelley, ...

The initiation of DNA replication at replication origins in eukaryotic cells is tightly controlled to ensure that the genome is duplicated only once each cell cycle. We present evidence that in...

The Role of fnx1, a Fission Yeast Multidrug Resistance Protein, in the Transition of Cells to a Quiescent G0 State

Dimitrov, Krassen, Sazer, Shelley

Most microorganisms live in conditions of nutrient limitation in their natural habitats. When exposed to these conditions they respond with physiological and morphological changes that enable them to...

The Ran GTPase System in Fission Yeast Affects Microtubules and Cytokinesis in Cells That Are Competent for Nucleocytoplasmic Protein Transport

Salus, Sandra S., Demeter, Janos, Sazer, Shelley

Misregulation of the evolutionarily conserved GTPase Ran in fission yeast results in defects in several cellular processes in cells that are competent for nucleocytoplasmic protein transport. These...

HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes

Chang, Fred, Re, Fabio, Sebastian, Sarah, Sazer, Shelley, Luban, Jeremy

Human immunodeficiency virus type 1 (HIV-1) Vpr is a 15-kDa accessory protein that contributes to several steps in the viral replication cycle and promotes virus-associated pathology. Previous...

The Fission Yeast Nup107-120 Complex Functionally Interacts with the Small GTPase Ran/Spi1 and Is Required for mRNA Export, Nuclear Pore Distribution, and Proper Cell Division

Baï, Siau Wei, Rouquette, Jacques, Umeda, Makoto, Faigle, Wolfgang, Loew, Damarys, Sazer, Shelley, ...

We have characterized Schizosaccharomyces pombe open reading frames encoding potential orthologues of constituents of the evolutionarily conserved Saccharomyces cerevisiae Nup84 vertebrate Nup107-160...

The A78V Mutation in the Mad3-like Domain of Schizosaccharomyces pombe Bub1p Perturbs Nuclear Accumulation and Kinetochore Targeting of Bub1p, Bub3p, and Mad3p and Spindle Assembly Checkpoint Function

Kadura, Sheila, He, Xiangwei, Vanoosthuyse, Vincent, Hardwick, Kevin G., Sazer, Shelley

During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby...

The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression

Umeda, Makoto, Izaddoost, Shahed, Cushman, Ian, Moore, Mary Shannon, Sazer, Shelley

The nuclear import of classical nuclear localization signal-containing proteins depends on importin-α transport receptors. In budding yeast there is a single importin-α gene and in higher...

Vesicle-Like Biomechanics Governs Important Aspects of Nuclear Geometry in Fission Yeast

Lim H. W., Gerald, Huber, Greg, Torii, Yoshihiro, Hirata, Aiko, Miller, Jonathan, Sazer, Shelley

It has long been known that during the closed mitosis of many unicellular eukaryotes, including the fission yeast (Schizosaccharomyces pombe), the nuclear envelope remains intact while the nucleus...

imp2, a New Component of the Actin Ring in the Fission Yeast Schizosaccharomyces pombe

Demeter, Janos, Sazer, Shelley

Cytokinesis is the part of the cell cycle in which the cell is cleaved to form two daughter cells. The unicellular yeast, Schizosaccharomyces pombe is an excellent model organism in which to study...

The Fission Yeast Ran Gtpase Is Required for Microtubule Integrity

Fleig, Ursula, Salus, Sandra S., Karig, Inga, Sazer, Shelley

The microtubule cytoskeleton plays a pivotal role in cytoplasmic organization, cell division, and the correct transmission of genetic information. In a screen designed to identify fission yeast genes...