Embryonic hair follicle fate change by augmented β-catenin through Shh and Bmp signaling (2009)
Suzuki, Kentaro, Yamaguchi, Yuji, Villacorte, Mylah, Mihara, Kenichiro, Akiyama, Masashi, Shimizu, Hitoshi, ...
β-catenin signaling is one of the key factors regulating the fate of hair follicles (HFs). To elucidate the regulatory mechanism of embryonic HF fate determination during epidermal...
Study of Androgen Receptor Functions by Genetic Models (2005)
Matsumoto, Takahiro, Takeyama, Ken-ichi, Sato, Takashi, Kato, Shigeaki
Androgens exert most of their biological activities through binding to the androgen receptor (AR). The AR belongs to the nuclear receptor superfamily and acts as a ligand-inducible transcriptional...
Colon-specific regulation of vitamin D hydroxylases--a possible approach for tumor prevention (2005)
Kállay, Enikö, Bises, Giovanna, Bajna, Erika, Bieglmayer, Christian, Gerdenitsch, Waltraud, Steffan, Ilse, ...
Epidemiological data suggest a protective role of calcium and vitamin D against colorectal tumor pathogenesis. 1,25-dihydroxyvitamin D3 (1,25-D3) is a key determinant of calcium homeostasis, cell...
1{alpha},25-Dihydroxyvitamin D3 is a preventive factor in the metastasis of lung cancer (2005)
Nakagawa, Kimie, Kawaura, Akihiko, Kato, Shigeaki, Takeda, Eiji, Okano, Toshio
1α,25-Dihydroxyvitamin D3 [1α,25(OH)2D3], the major regulator of calcium homeostasis, has potent antiproliferative and anti-invasive properties in vitro in cancer cells. Studies in vivo...
22-oxa-1{alpha},25-dihydroxyvitamin D3 inhibits metastasis and angiogenesis in lung cancer (2005)
Nakagawa, Kimie, Sasaki, Yuko, Kato, Shigeaki, Kubodera, Noboru, Okano, Toshio
1α,25-Dihydroxyvitamin D3 (1α,25-D3) has potent antiproliferative and anti-invasive properties in vitro in cancer cells. However, its calcemic effect in vivo limits its therapeutic applications....
Kállay, Enikö, Bises, Giovanna, Bajna, Erika, Bieglmayer, Christian, Gerdenitsch, Waltraud, Steffan, Ilse, ...
Epidemiological data suggest a protective role of calcium and vitamin D against colorectal tumor pathogenesis. 1,25-dihydroxyvitamin D3 (1,25-D3) is a key determinant of calcium homeostasis, cell...
22-Oxa-1{alpha},25-dihydroxyvitamin D3 inhibits metastasis and angiogenesis in lung cancer (2005)
Nakagawa, Kimie, Sasaki, Yuko, Kato, Shigeaki, Kubodera, Noboru, Okano, Toshio
1α,25-Dihydroxyvitamin D3 (1α,25-D3) has potent antiproliferative and anti-invasive properties in vitro in cancer cells. However, its calcemic effect in vivo limits its therapeutic applications....
1{alpha},25-dihydroxyvitamin D3 is a preventive factor in the metastasis of lung cancer (2004)
Nakagawa, Kimie, Kawaura, Akihiko, Kato, Shigeaki, Takeda, Eiji, Okano, Toshio
1α,25-Dihydroxyvitamin D3 [1α,25(OH)2D3], the major regulator of calcium homeostasis, has potent antiproliferative and anti-invasive properties in vitro in cancer cells. Studies in vivo...
Requirements for FGF3 and FGF10 during inner ear formation (2003)
Alvarez, Yolanda, Alonso, Maria Teresa, Vendrell, Victor, Zelarayan, Laura Cecilia, Chamero, Pablo, Theil, Thomas, ...
Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be...
Requirement of fibroblast growth factor 10 in development of white adipose tissue (2002)
Sakaue, Hiroshi, Konishi, Morichika, Ogawa, Wataru, Asaki, Toshiyuki, Mori, Toshiyuki, Yamasaki, Masahiro, ...
Matsui, Daisuke, Sakari, Matomo, Sato, Takashi, Murayama, Akiko, Takada, Ichiro, Kim, Misun, ...
The steroid 5α-reductase (5α-R) plays an important physiological role in the conversion of steroid hormones such as androgen and progesterone to their 5α-reduced derivatives. 5α-R type II...
Lee, Haeng-Seog, Miyauchi, Keisuke, Nagata, Yuji, Fukuda, Ryouichi, Sasagawa, Shin-ichi, Endoh, Hideki, ...
To screen a wide variety of chemicals for endocrine disrupters, and to develop an effective microbial degradation system for them, a good system is needed for the rapid and accurate evaluation of the...
In vitro and in vivo analysis of the immune system of vitamin D receptor knockout mice (2001)
Kato, Shigeaki, ...
Immune cells carry receptors for 1,25-dihydroxyvitamin D3 [1,25(OH)2D3; vitamin D receptor (VDR)] and individuals with severe vitamin D deficiency have immune abnormalities. The aim of this study was...
Kallay, Enikö, Pietschmann, Peter, Toyokuni, Shinya, Bajna, Erika, Hahn, Philipp, Mazzucco, Karl, ...
The vitamin D receptor knockout (VDR-KO) mouse presents with a skeletal phenotype typical for complete lack of genomic 1,25-dihydroxycholecalciferol effects. Our previous data from human colorectal...
The Function of Vitamin D Receptor in Vitamin D Action (2000)
Vitamin D has roles in a variety of biological actions such as calcium homeostasis, cell proliferation and cell differentiation to many target tissues. Most of these biological actions of vitamin D...
Arao, Yukitomo, Miyatake, Naoto, Yamamoto, Etsuko, Usiku, Hiroaki, Matsuura, Akiko, Ninomiya, Yuichi, ...
The stabilization of chicken ovalbumin (OVA) mRNA by different classes of steroid hormones (estrogen, progesterone, glucocorticoid, and androgen) was studied in the oviducts of chicks treated with...
Kato, Ko, Makino, Yasutaka, Kishimoto, Toshihiko, Yamauchi, Jun, Kato, Shigeaki, Muramatsu, Masami, ...
The conformatioal states of the mouse TATA-binding protein (TBP) in solution were studied. A histldlne tag and a factor Xa recognition site-carrying mouse TBP was expressed in E.coli, highly...
Kato, Shigeaki, Yamagishi, Kozo, Tatsuzawa, Fumi, Suzuki, Kazuo, Takano, Saburo, Eguchi, Masahiro, ...
Several low-molecular-weight heat-shock proteins were detected by two-dimensional electro-phoresis in substantial amounts in both nuclear and cytosolic extracts of tomato fruit.
Ligand-induced transrepression by VDR through association of WSTF with acetylated histones
Fujiki, Ryoji, Kim, Mi-sun, Sasaki, Yasumasa, Yoshimura, Kimihiro, Kitagawa, Hirochika, Kato, Shigeaki
We have previously shown that the novel ATP-dependent chromatin-remodeling complex WINAC is required for the ligand-bound vitamin D receptor (VDR)-mediated transrepression of the 25(OH)D3...
Duodenal calcium absorption in vitamin D receptor–knockout mice: Functional and molecular aspects
Van Cromphaut, Sophie J., Dewerchin, Mieke, Hoenderop, Joost G. J., Stockmans, Ingrid, Van Herck, Erik, Kato, Shigeaki, ...
Rickets and hyperparathyroidism caused by a defective vitamin D receptor (VDR) can be prevented in humans and animals by high calcium intake, suggesting that intestinal calcium absorption is critical...
Endoh, Hideki, Maruyama, Kazunori, Masuhiro, Yoshikazu, Kobayashi, Yoko, Goto, Masahide, Tai, Hitoshi, ...
The estrogen receptor (ER) regulates the expression of target genes in a ligand-dependent manner. The ligand-dependent activation function AF-2 of the ER is located in the ligand binding domain...
Um, Moonkyoung, Yamauchi, Jun, Kato, Shigeaki, Manley, James L.
TATA-binding protein (TBP) is a key general transcription factor required for transcription by all three nuclear RNA polymerases. Although it has been intensively analyzed in vitro and in...
Transcriptional regulation of the mouse steroid 5α-reductase type II gene by progesterone in brain
Matsui, Daisuke, Sakari, Matomo, Sato, Takashi, Murayama, Akiko, Takada, Ichiro, Kim, Misun, ...
The steroid 5α-reductase (5α-R) plays an important physiological role in the conversion of steroid hormones such as androgen and progesterone to their 5α-reduced derivatives. 5α-R type II...
Selective Interaction of Vitamin D Receptor with Transcriptional Coactivators by a Vitamin D Analog
Takeyama, Ken-Ichi, Masuhiro, Yoshikazu, Fuse, Hiroaki, Endoh, Hideki, Murayama, Akiko, Kitanaka, Sachiko, ...
The nuclear vitamin D receptor (VDR) is a member of a nuclear receptor superfamily and acts as a ligand-dependent transcription factor. A family of cotranscriptional activators (SRC-1, TIF2, and...
Kitagawa, Hirochika, Yanagisawa, Junn, Fuse, Hiroaki, Ogawa, Satoko, Yogiashi, Yoshiko, Okuno, Atsuro, ...
The rat mineralocorticoid receptor (MR) has two activation functions in distinct regions of the A/B domain, designated activation function 1a (AF-1a; amino acids 1 to 169) and AF-1b (amino acids 451...
Watanabe, Michiko, Yanagisawa, Junn, Kitagawa, Hirochika, Takeyama, Ken-ichi, Ogawa, Satoko, Arao, Yukitomo, ...
One class of the nuclear receptor AF-2 coactivator complexes contains the SRC-1/TIF2 family, CBP/p300 and an RNA coactivator, SRA. We identified a subfamily of RNA-binding DEAD-box proteins (p72/p68)...
Chondromodulin I Is a Bone Remodeling Factor
Nakamichi, Yuko, Shukunami, Chisa, Yamada, Takashi, Aihara, Ken-ichi, Kawano, Hirotaka, Sato, Takashi, ...
Chondromodulin I (ChM-I) was supposed from its limited expression in cartilage and its functions in cultured chondrocytes as a major regulator in cartilage development. Here, we generated mice...
Requirement of fibroblast growth factor 10 in development of white adipose tissue
Sakaue, Hiroshi, Konishi, Morichika, Ogawa, Wataru, Asaki, Toshiyuki, Mori, Toshiyuki, Yamasaki, Masahiro, ...
Fibroblast growth factors (FGFs) are important intercellular signaling molecules in developmental processes. Here, we show that FGF10 is secreted by cultured preadipocytes and that prevention of...
Suppressive function of androgen receptor in bone resorption
Kawano, Hirotaka, Sato, Takashi, Yamada, Takashi, Matsumoto, Takahiro, Sekine, Keisuke, Watanabe, Tomoyuki, ...
As locally converted estrogen from testicular testosterone contributes to apparent androgen activity, the physiological significance of androgen receptor (AR) function in the beneficial effects of...
Inhibition of RXR and PPARγ ameliorates diet-induced obesity and type 2 diabetes
Yamauchi, Toshimasa, Waki, Hironori, Kamon, Junji, Murakami, Koji, Motojima, Kiyoto, Komeda, Kajuro, ...
PPARγ is a ligand-activated transcription factor and functions as a heterodimer with a retinoid X receptor (RXR). Supraphysiological activation of PPARγ by thiazolidinediones can reduce insulin...
Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein
Yahata, Tetsuro, Shao, Wenlin, Endoh, Hideaki, Hur, Jingyung, Coser, Kathryn R., Sun, Huiping, ...
CITED1, a CBP/p300-binding nuclear protein that does not bind directly to DNA, is a transcriptional coregulator. Here, we show evidence that CITED1 functions as a selective coactivator for...
Brain masculinization requires androgen receptor function
Sato, Takashi, Matsumoto, Takahiro, Kawano, Hirotaka, Watanabe, Tomoyuki, Uematsu, Yoshikatsu, Sekine, Keisuke, ...
Testicular testosterone produced during a critical perinatal period is thought to masculinize and defeminize the male brain from the inherent feminization program and induce male-typical behaviors in...
Transrepression by a liganded nuclear receptor via a bHLH activator through co-regulator switching
Murayama, Akiko, Kim, Mi-sun, Yanagisawa, Junn, Takeyama, Ken-ichi, Kato, Shigeaki
Vitamin D receptor (VDR) is essential for ligand-induced gene repression of 25(OH)D3 1α-hydroxylase (1α(OH)ase) in mammalian kidney, while this gene expression is activated by protein kinase A...
Ligand-dependent switching of ubiquitin–proteasome pathways for estrogen receptor
Tateishi, Yukiyo, Kawabe, Yoh-ichi, Chiba, Tomoki, Murata, Shigeo, Ichikawa, Ken, Murayama, Akiko, ...
Recent evidence indicates that the transactivation of estrogen receptor α (ERα) requires estrogen-dependent receptor ubiquitination and degradation. Here we show that estrogen-unbound (unliganded)...
Neuronal Leucine-Rich Repeat Protein 4 Functions in Hippocampus-Dependent Long-Lasting Memory
Bando, Takayoshi, Sekine, Keisuke, Kobayashi, Shizuka, Watabe, Ayako M., Rump, Armin, Tanaka, Minoru, ...
Neuronal leucine-rich repeat proteins (NLRRs) are type I transmembrane proteins and expressed in neuronal tissues, but their function remains unknown. Here, we describe the identification and...
Splicing potentiation by growth factor signals via estrogen receptor phosphorylation
Masuhiro, Yoshikazu, Mezaki, Yoshihiro, Sakari, Matomo, Takeyama, Ken-ichi, Yoshida, Tasuku, Inoue, Kunio, ...
Mitogen-activated protein kinase-mediated growth factor signals are known to augment the ligand-induced transactivation function of nuclear estrogen receptor α (ERα) through phosphorylation of...
Role of the vitamin D receptor in FGF23 action on phosphate metabolism
Inoue, Yoshio, Segawa, Hiroko, Kaneko, Ichiro, Yamanaka, Setsuko, Kusano, Kenichiro, Kawakami, Eri, ...
FGF23 (fibroblast growth factor 23) is a novel phosphaturic factor that influences vitamin D metabolism and renal re-absorption of Pi. The goal of the present study was to characterize the role of...
Ligand-induced transrepression by VDR through association of WSTF with acetylated histones
Fujiki, Ryoji, Kim, Mi-sun, Sasaki, Yasumasa, Yoshimura, Kimihiro, Kitagawa, Hirochika, Kato, Shigeaki
We have previously shown that the novel ATP-dependent chromatin-remodeling complex WINAC is required for the ligand-bound vitamin D receptor (VDR)-mediated transrepression of the 25(OH)D3...
Premature ovarian failure in androgen receptor-deficient mice
Shiina, Hiroko, Matsumoto, Takahiro, Sato, Takashi, Igarashi, Katsuhide, Miyamoto, Junko, Takemasa, Sayuri, ...
Premature ovarian failure (POF) syndrome, an early decline of ovarian function in women, is frequently associated with X chromosome abnormalities ranging from various Xq deletions to complete loss of...
Ma, Yanfei, Khalifa, Berket, Yee, Ying K., Lu, Jianfen, Memezawa, Ai, Savkur, Rajesh S., ...
Vitamin D receptor (VDR) ligands are therapeutic agents for the treatment of psoriasis, osteoporosis, and secondary hyperparathyroidism. VDR ligands also show immense potential as therapeutic agents...
Li, Mei, Hener, Pierre, Zhang, Zhikun, Kato, Shigeaki, Metzger, Daniel, Chambon, Pierre
We have demonstrated that cytokine thymic stromal lymphopoietin (TSLP), whose expression is rapidly induced upon keratinocyte-selective ablation of retinoid X receptors (RXRs) -α and -β in the...
Tateishi, Yukiyo, Sonoo, Raku, Sekiya, Yu-ichi, Sunahara, Nanae, Kawano, Miwako, Wayama, Mitsutoshi, ...
Recent studies have shed light on the ligand-dependent transactivation mechanisms of nuclear receptors (NRs). When the ligand dose is reduced, the transcriptional activity of NRs should be...
Aihara, Ken-ichi, Azuma, Hiroyuki, Akaike, Masashi, Ikeda, Yasumasa, Sata, Masataka, Takamori, Nobuyuki, ...
Heparin cofactor II (HCII) specifically inhibits thrombin action at sites of injured arterial wall, and patients with HCII deficiency exhibit advanced atherosclerosis. However, the in vivo effects...
The Pituitary Function of Androgen Receptor Constitutes a Glucocorticoid Production Circuit▿
Miyamoto, Junko, Matsumoto, Takahiro, Shiina, Hiroko, Inoue, Kazuki, Takada, Ichiro, Ito, Saya, ...
Androgen receptor (AR) mediates diverse androgen actions, particularly reproductive processes in males and females. AR-mediated androgen signaling is considered to also control metabolic processes;...
A cell cycle-dependent co-repressor mediates photoreceptor cell-specific nuclear receptor function
Takezawa, Shinichiro, Yokoyama, Atsushi, Okada, Maiko, Fujiki, Ryoji, Iriyama, Aya, Yanagi, Yasuo, ...
Photoreceptor cell-specific nuclear receptor (PNR) (NR2E3) acts as a sequence-specific repressor that controls neuronal differentiation in the developing retina. We identified a novel PNR...
Kitagawa , Hirochika, Ray , William J., Glantschnig, Helmut, Nantermet, Pascale V., Yu, Yuanjiang, Leu, Chih-Tai, ...
The orphan nuclear receptor Nurr1 is essential for the development and maintenance of midbrain dopaminergic neurons, the cells that degenerate during Parkinson's disease, by promoting the...
Igarashi, Mamoru, Yogiashi, Yoshiko, Mihara, Masatomo, Takada, Ichiro, Kitagawa, Hirochika, Kato, Shigeaki
Vitamin K is a fat-soluble vitamin that serves as a coenzyme for vitamin K-dependent carboxylase. Besides its canonical action, vitamin K binds to the steroid and xenobiotic receptor (SXR)/pregnane X...
Fujita, Hiroki, Sugimoto, Kotaro, Inatomi, Shuichiro, Maeda, Toshihiro, Osanai, Makoto, Uchiyama, Yasushi, ...
Ca2+ is absorbed across intestinal epithelial monolayers via transcellular and paracellular pathways, and an active form of vitamin D3, 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], is known to promote...
Transrepressive Function of TLX Requires the Histone Demethylase LSD1 ▿ †
Yokoyama, Atsushi, Takezawa, Shinichiro, Schüle, Roland, Kitagawa, Hirochika, Kato, Shigeaki
TLX is an orphan nuclear receptor (also called NR2E1) that regulates the expression of target genes by functioning as a constitutive transrepressor. The physiological significance of TLX in the...
Switching of chromatin-remodelling complexes for oestrogen receptor-α
Okada, Maiko, Takezawa, Shin-ichiro, Mezaki, Yoshihiro, Yamaoka, Ikuko, Takada, Ichiro, Kitagawa, Hirochika, ...
The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen...
Coactivation of Estrogen Receptor β by Gonadotropin-Induced Cofactor GIOT-4 ▿
Kouzu-Fujita, Madoka, Mezaki, Yoshihiro, Sawatsubashi, Shun, Matsumoto, Takahiro, Yamaoka, Ikuko, Yano, Tetsu, ...
Estrogen exerts its diverse effects through two subtypes of estrogen receptors (ER), ERα and ERβ. Each subtype has its own distinct function and expression pattern in its target tissues. Little,...
Zhang, Zhikun, Hener, Pierre, Frossard, Nelly, Kato, Shigeaki, Metzger, Daniel, Li, Mei, ...
Atopic dermatitis (AD) is often the initial step in the “atopic march,” given that more than half of AD patients with moderate to severe AD develop asthma later in life. Both AD and asthma share...
Zhao, Yue, Takeyama, Ken-ichi, Sawatsubashi, Shun, Ito, Saya, Suzuki, Eriko, Yamagata, Kaoru, ...
Ligand-bound nuclear receptors (NR) activate transcription of the target genes. This activation is coupled with histone modifications and chromatin remodeling through the function of various...
Aberrant E2F activation by polyglutamine expansion of androgen receptor in SBMA neurotoxicity
Suzuki, Eriko, Zhao, Yue, Ito, Saya, Sawatsubashi, Shun, Murata, Takuya, Furutani, Takashi, ...
Spinal and bulbar muscular atrophy (SBMA) is a neurodegenerative disorder caused by a polyglutamine repeat (polyQ) expansion within the human androgen receptor (AR). Unlike other neurodegenerative...
Iwasawa, Mitsuyasu, Miyazaki, Tsuyoshi, Nagase, Yuichi, Akiyama, Toru, Kadono, Yuho, Nakamura, Masaki, ...
The B cell lymphoma 2 (Bcl-2) family member Bcl-xL has a well-characterized antiapoptotic function in lymphoid cells. However, its functions in other cells — including osteoclasts, which are of...