Mimida, Naozumi, Kotoda, Nobuhiro, Ueda, Takanori, Igarashi, Megumi, Hatsuyama, Yoshimichi, Iwanami, Hiroshi, ...
Recent molecular analyses in several plant species revealed that TERMINAL FLOWER1 (TFL1) and CENTRORADIALIS (CEN) homologs are involved in regulating the flowering time and/or maintaining the...
Mechanism of Anucleate Cell Production in the oriC-Deleted Mutants of Bacillus subtilis (1997)
Moriya, Shigeki, Hassan, Anwarul K. M., Kadoya, Ryosuke, Ogasawara, Naotake
We constructed oriC-deleted mutants of Bacillus subtilis by integrating the minimal replication region of plasmid pLS32 into the proA (115°), spoIIIJ (360°) and thrS (256°) loci of the chromosome,...
Hassan, Anwarul KM, Moriya, Shigeki, Baumann, Paul, Yoshikawa, Hiroshi, Ogasawara, Naotake
Buchnera aphidicola is an intracellular prokaryote (endosymbiont) that lives in the body cavity of the aphid. Phylogenetic studies indicated that it is closely related to Escherichia coli and members...
Fukuoka, Takuya, Moriya, Shigeki, Yoshikawa, Hiroshi, Ogasawara, Naotake
Bacillus subtilis DnaA protein was overproduced by a recombinant plasmid containing B. subtilis dnaA gene in a mutant Escherichia coli strain which is deficient in its own DnaA and RNaseH. The...
Ogasawara, Naotake, Moriya, Shigeki, Mazza, Pier Giorgio, Yoshikawa, Hiroshi
A region of the Bacillus subtilis chromosome containing dnaB, a gene essential for the initiation of chromosomal replication in B. subtilis, was cloned and nucleotide sequence of some 5000bp...
Moriya, Shigeki, Ogasawara, Naotake, Yoshikawa, Hiroshi
Approximately 10,000 nucleotides were sequenced in the oriC region of the Bacillue subtilis chromosome. The first replicating DNA strands are hybridized with a SalI-EcoRI fragment (nucleotide...
Ogasawara, Naotake, Moriya, Shigeki, Yoshikawa, Hiroshi
We have determined nucleotide sequence of some 10,000 base pairs (bp) in the oriC region of the Bacillus subtilis chromosome. Initiation sites of transcription from this region were determined in...
Ogasawara, Naotake, Moriya, Shigeki, Yoshikawa, Hiroshi
Structure and organization of two complete ribosomal RNA (rRNA) gene sets, rrn0 and rrnA, were determined for the first time in Bacillus subtilis. They are located at the region of the replication...
DnaD Protein of Bacillus subtilis Interacts with DnaA, the Initiator Protein of Replication
Ishigo-oka, Daisuke, Ogasawara, Naotake, Moriya, Shigeki
The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23...
Ogura, Yoshitoshi, Imai, Yukiho, Ogasawara, Naotake, Moriya, Shigeki
In Escherichia coli, the DnaA protein level appears to play a pivotal role in determining the timing of replication initiation. To examine the effects on replication initiation in B. subtilis, we...
Lee, Philina S., Lin, Daniel Chi-Hong, Moriya, Shigeki, Grossman, Alan D.
Spo0J (ParB) of Bacillus subtilis is a DNA-binding protein that belongs to a conserved family of proteins required for efficient plasmid and chromosome partitioning in many bacterial species. We...
Increasing the Ratio of Soj to Spo0J Promotes Replication Initiation in Bacillus subtilis
Ogura, Yoshitoshi, Ogasawara, Naotake, Harry, Elizabeth J., Moriya, Shigeki
The ParA and ParB protein families are well conserved in bacteria. However, their functions are still unclear. In Bacillus subtilis, Soj and Spo0J are members of these two protein families,...
Kitagawa, Risa, Ozaki, Toru, Moriya, Shigeki, Ogawa, Tohru
Replication of the Escherichia coli chromosome is initiated at a unique site, oriC. Concurrent initiation occurs at all oriC sites present in a cell once, and only once, per cell cycle. A mechanism...
DnaD Protein of Bacillus subtilis Interacts with DnaA, the Initiator Protein of Replication
Ishigo-oka, Daisuke, Ogasawara, Naotake, Moriya, Shigeki
The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23...
Ogura, Yoshitoshi, Imai, Yukiho, Ogasawara, Naotake, Moriya, Shigeki
In Escherichia coli, the DnaA protein level appears to play a pivotal role in determining the timing of replication initiation. To examine the effects on replication initiation in B. subtilis, we...
Lee, Philina S., Lin, Daniel Chi-Hong, Moriya, Shigeki, Grossman, Alan D.
Spo0J (ParB) of Bacillus subtilis is a DNA-binding protein that belongs to a conserved family of proteins required for efficient plasmid and chromosome partitioning in many bacterial species. We...
Increasing the Ratio of Soj to Spo0J Promotes Replication Initiation in Bacillus subtilis
Ogura, Yoshitoshi, Ogasawara, Naotake, Harry, Elizabeth J., Moriya, Shigeki
The ParA and ParB protein families are well conserved in bacteria. However, their functions are still unclear. In Bacillus subtilis, Soj and Spo0J are members of these two protein families,...
Kitagawa, Risa, Ozaki, Toru, Moriya, Shigeki, Ogawa, Tohru
Replication of the Escherichia coli chromosome is initiated at a unique site, oriC. Concurrent initiation occurs at all oriC sites present in a cell once, and only once, per cell cycle. A mechanism...