Yamashiro, Shigeko, Yamakita, Yoshihiko, Ono, Shoichiro, Matsumura, Fumio
Fascin is an actin-bundling protein that is found in membrane ruffles, microspikes, and stress fibers. The expression of fascin is greatly increased in many transformed cells, as well as in...
Cell-Matrix Adhesions Differentially Regulate Fascin Phosphorylation
Adams, Josephine C., Clelland, James D., Collett, Georgina D.M., Matsumura, Fumio, Yamashiro, Shigeko, Zhang, Linglan
Cell adhesion to individual macromolecules of the extracellular matrix has dramatic effects on the subcellular localization of the actin-bundling protein fascin and on the ability of cells to form...
Mutant Caldesmon Lacking cdc2 Phosphorylation Sites Delays M-Phase Entry and Inhibits Cytokinesis
Yamashiro, Shigeko, Chern, Hueylan, Yamakita, Yoshihiko, Matsumura, Fumio
Caldesmon is phosphorylated by cdc2 kinase during mitosis, resulting in the dissociation of caldesmon from microfilaments. To understand the physiological significance of phosphorylation, we...
Yamashiro, Shigeko, Totsukawa, Go, Yamakita, Yoshihiko, Sasaki, Yasuharu, Madaule, Pascal, Ishizaki, Toshimaa, ...
Citron kinase is a Rho-effector protein kinase that is related to Rho-associated kinases of ROCK/ROK/Rho-kinase family. Both ROCK and citron kinase are suggested to play a role in cytokinesis....
Yamashiro, Shigeko, Yamakita, Yoshihiko, Ono, Shoichiro, Matsumura, Fumio
Fascin is an actin-bundling protein that is found in membrane ruffles, microspikes, and stress fibers. The expression of fascin is greatly increased in many transformed cells, as well as in...
Cell-Matrix Adhesions Differentially Regulate Fascin Phosphorylation
Adams, Josephine C., Clelland, James D., Collett, Georgina D.M., Matsumura, Fumio, Yamashiro, Shigeko, Zhang, Linglan
Cell adhesion to individual macromolecules of the extracellular matrix has dramatic effects on the subcellular localization of the actin-bundling protein fascin and on the ability of cells to form...
Mutant Caldesmon Lacking cdc2 Phosphorylation Sites Delays M-Phase Entry and Inhibits Cytokinesis
Yamashiro, Shigeko, Chern, Hueylan, Yamakita, Yoshihiko, Matsumura, Fumio
Caldesmon is phosphorylated by cdc2 kinase during mitosis, resulting in the dissociation of caldesmon from microfilaments. To understand the physiological significance of phosphorylation, we...
Yamashiro, Shigeko, Totsukawa, Go, Yamakita, Yoshihiko, Sasaki, Yasuharu, Madaule, Pascal, Ishizaki, Toshimaa, ...
Citron kinase is a Rho-effector protein kinase that is related to Rho-associated kinases of ROCK/ROK/Rho-kinase family. Both ROCK and citron kinase are suggested to play a role in cytokinesis....
Totsukawa, Go, Wu, Yue, Sasaki, Yasuharu, Hartshorne, David J., Yamakita, Yoshihiko, Yamashiro, Shigeko, ...
We examined the role of regulatory myosin light chain (MLC) phosphorylation of myosin II in cell migration of fibroblasts. Myosin light chain kinase (MLCK) inhibition blocked MLC phosphorylation at...
Matsumura, Fumio, Ono, Shoichiro, Yamakita, Yoshihiko, Totsukawa, Go, Yamashiro, Shigeko
Phosphorylation of the regulatory light chain of myosin II (RMLC) at Serine 19 by a specific enzyme, MLC kinase, is believed to control the contractility of actomyosin in smooth muscle and vertebrate...
Yamakita, Yoshihiko, Totsukawa, Go, Yamashiro, Shigeko, Fry, David, Zhang, Xiaoe, Hanks, Steven K., ...
At mitosis, focal adhesions disassemble and the signal transduction from focal adhesions is inactivated. We have found that components of focal adhesions including focal adhesion kinase (FAK),...
Activation of Myosin Phosphatase Targeting Subunit by Mitosis-specific Phosphorylation
Totsukawa, Go, Yamakita, Yoshihiko, Yamashiro, Shigeko, Hosoya, Hiroshi, Hartshorne, David J., Matsumura, Fumio
It has been demonstrated previously that during mitosis the sites of myosin phosphorylation are switched between the inhibitory sites, Ser 1/2, and the activation sites, Ser 19/Thr 18 (Yamakita, Y.,...
Totsukawa, Go, Yamakita, Yoshihiko, Yamashiro, Shigeko, Hartshorne, David J., Sasaki, Yasuharu, Matsumura, Fumio
ROCK (Rho-kinase), an effector molecule of RhoA, phosphorylates the myosin binding subunit (MBS) of myosin phosphatase and inhibits the phosphatase activity. This inhibition increases phosphorylation...