Shigeo Yoshida

Plant Cells Without Detectable Plastids are Generated in the crumpled leaf Mutant of Arabidopsis thaliana (2009)

Chen, Yuling, Asano, Tomoya, Fujiwara, Makoto T., Yoshida, Shigeo, Machida, Yasunori, Yoshioka, Yasushi

Plastids are maintained in cells by proliferating prior to cell division and being partitioned to each daughter cell during cell division. It is unclear, however, whether cells without plastids are...

Shooting control by brassinosteroids: metabolomic analysis and effect of brassinazole on Malus prunifolia, the Marubakaido apple rootstock (2009)

Pereira-Netto, Adaucto B., Roessner, Ute, Fujioka, Shozo, Bacic, Antony, Asami, Tadao, Yoshida, Shigeo, ...

To help unravel the role of brassinosteroids (BRs) in the control of shooting, we treated the shoots of Marubakaido apple rootstock (Malus prunifolia (Willd.) Borkh cv. Marubakaido) with brassinolide...

Involvement of C-22-Hydroxylated Brassinosteroids in Auxin-Induced Lamina Joint Bending in Rice (2009)

Nakamura, Ayako, Fujioka, Shozo, Takatsuto, Suguru, Tsujimoto, Masafumi, Kitano, Hidemi, Yoshida, Shigeo, ...

The rice lamina joint is ideal material for investigating the activity of brassinosteroids (BRs) and auxin because of its high sensitivity to these compounds. Using a series of rice BR biosynthetic...

A Double Mutant for the CYP85A1 and CYP85A2 Genes of Arabidopsis Exhibits a Brassinosteroid Dwarf Phenotype (2008)

Kwon, Mi, Fujioka, Shozo, Jeon, Ji Hyun, Kim, Ho Bang, Takatsuto, Suguru, Yoshida, Shigeo, ...

Brassinosteroid (BR)-6-oxidases mediate the bridge reactions that connect the late and early C-6 oxidation pathways by converting 6-deoxoBR to 6-oxoBRs. Two similar genes of Arabidopsis, CYP85A1...

An Arabidopsis short root and dwarfism Mutant Defines a Novel Locus That Mediates Both Cell Division and Elongation (2008)

Lee, Hyun-Kyung, Kwon, Mi, Jeon, Ji-Hyun, Fujioka, Shozo, Kim, Ho-Bang, Park, So-Young, ...

Arabidopsis leaf morphology is determined by the coordinated action of cell division and elongation. Of all the hormones that control leaf shape, the brassinosteroids (BRs) are active components in...

The Assembly of the FtsZ Ring at the Mid-Chloroplast Division Site Depends on a Balance Between the Activities of AtMinE1 and ARC11/AtMinD1 (2008)

Fujiwara, Makoto T., Hashimoto, Haruki, Kazama, Yusuke, Abe, Tomoko, Yoshida, Shigeo, Sato, Naoki, ...

Chloroplast division comprises a sequence of events that facilitate symmetric binary fission and that involve prokaryotic-like stromal division factors such as tubulin-like GTPase FtsZ and the...

LOVASTATIN INSENSITIVE 1, a Novel Pentatricopeptide Repeat Protein, is a Potential Regulatory Factor of Isoprenoid Biosynthesis in Arabidopsis (2007)

Kobayashi, Keiko, Suzuki, Masashi, Tang, Jianwei, Nagata, Noriko, Ohyama, Kiyoshi, Seki, Hikaru, ...

Higher plants have two metabolic pathways for isoprenoid biosynthesis: the cytosolic mevalonate (MVA) pathway and the plastidal non-mevalonate (MEP) pathway. Despite the compartmentalization of these...

Co-Regulation of Brassinosteroid Biosynthesis-Related Genes During Xylem Cell Differentiation (2007)

Yamamoto, Ryo, Fujioka, Shozo, Iwamoto, Kuninori, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, ...

To understand the regulatory mechanisms of brassinosteroid (BR) biosynthesis in specific plant developmental processes, we first investigated the accumulation profiles of BRs and sterols in xylem...

吉田 茂生 (2005)

吉田,茂生, ヨシダ,シゲオ, Yoshida,Shigeo

Shigeo Yoshida's Homepage,三島由紀夫,近代能楽集 弱法師,Go to the,page,過去のおことば集

Mechanisms of aging in senescence-accelerated mice (2005)

Carter, Todd A, Greenhall, Jennifer A, Yoshida, Shigeo, Fuchs, Sebastian, Helton, Robert, Swaroop, Anand, ...

Abstract Background Progressive neurological dysfunction is a key aspect of human aging. Because of underlying differences in the aging of mice and humans, useful mouse models have been difficult to...

Ectopic endoreduplication caused by sterol alteration results in serrated petals in Arabidopsis (2005)

Hase, Yoshihiro, Fujioka, Shozo, Yoshida, Shigeo, Sun, Guoqing, Umeda, Masaaki, Tanaka, Atsushi

The Arabidopsis frill1 (frl1) mutant, that has serrated petals and sepals but no other large changes in plant morphology, was studied. The frl1 had a mutation in STEROL METHYLTRANSFERASE 2 and an...

Identification of a Novel Factor, Vanillyl Benzyl Ether, Which Inhibits Somatic Embryogenesis of Japanese Larch (Larix leptolepis Gordon) (2005)

Umehara, Mikihisa, Ogita, Shinjiro, Sasamoto, Hamako, Koshino, Hiroyuki, Asami, Tadao, Fujioka, Shozo, ...

In contrast to angiosperms, some gymnosperms form well-developed suspensors in somatic embryogenesis. This characteristic makes it easy to study suspensor biology. In cultures with high cell...

Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules (2005)

Sun, Yan, Veerabomma, Suresh, Abdel-Mageed, Haggag A., Fokar, Mohamed, Asami, Tadao, Yoshida, Shigeo, ...

Our current understanding of the role of phytohormones in the development of cotton fibers is derived largely from an amenable culture system in which cotton ovules, collected on the day of anthesis,...

Ectopic endoreduplication caused by sterol alteration results in serrated petals in Arabidopsis (2005)

Hase, Yoshihiro, Fujioka, Shozo, Yoshida, Shigeo, Sun, Guoqing, Umeda, Masaaki, Tanaka, Atsushi

The Arabidopsis frill1 (frl1) mutant, that has serrated petals and sepals but no other large changes in plant morphology, was studied. The frl1 had a mutation in STEROL METHYLTRANSFERASE 2 and an...

Identification of a Novel factor, Vanillyl Benzyl Ether, Which Inhibits Somatic Embryogenesis of Japanese Larch (Larix leptolepis Gordon) (2005)

Umehara, Mikihisa, Ogita, Shinjiro, Sasamoto, Hamako, Koshino, Hiroyuki, Asami, Tadao, Fujioka, Shozo, ...

In contrast to angiosperms, some gymnosperms form well-developed suspensors in somatic embryogenesis. This characteristic makes it easy to study suspensor biology. In cultures with high cell...

Ectopic endoreduplication caused by sterol alteration results in serrated petals in Arabidopsis (2005)

Hase, Yoshihiro, Fujioka, Shozo, Yoshida, Shigeo, Sun, Guoqing, Umeda, Masaaki, Tanaka, Atsushi

The Arabidopsis frill1 (frl1) mutant, that has serrated petals and sepals but no other large changes in plant morphology, was studied. The frl1 had a mutation in STEROL METHYLTRANSFERASE 2 and an...

Identification of a Novel Factor, Vanillyl Benzyl Ether, Which Inhibits Somatic Embryogenesis of Japanese Larch (Larix leptolepis Gordon) (2005)

Umehara, Mikihisa, Ogita, Shinjiro, Sasamoto, Hamako, Koshino, Hiroyuki, Asami, Tadao, Fujioka, Shozo, ...

In contrast to angiosperms, some gymnosperms form well-developed suspensors in somatic embryogenesis. This characteristic makes it easy to study suspensor biology. In cultures with high cell...

Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules (2005)

Sun, Yan, Veerabomma, Suresh, Abdel-Mageed, Haggag A., Fokar, Mohamed, Asami, Tadao, Yoshida, Shigeo, ...

Our current understanding of the role of phytohormones in the development of cotton fibers is derived largely from an amenable culture system in which cotton ovules, collected on the day of anthesis,...

Expression profiling of the developing and mature Nrl-/- mouse retina: Identification of retinal disease candidates and transcriptional regulatory targets of Nrl (2004)

Yoshida, Shigeo, Mears, Alan J., Friedman, James S., Carter, Todd, He, Shirley, Oh, Edwin, ...

The rod photoreceptor-specific neural retina leucine zipper protein Nrl is essential for rod differentiation and plays a critical role in regulating gene expression. In the mouse retina, rods account...

Control of Nodule Number by the Phytohormone Abscisic Acid in the Roots of Two Leguminous Species (2004)

Suzuki, Akihiro, Akune, Mitsumi, Kogiso, Mari, Imagama, Yoshihiro, Osuki, Ken-ichi, Uchiumi, Toshiki, ...

The effects of the phytohormone abscisic acid (ABA) on plant growth and root nodule formation were analyzed in Trifolium repense (white clover) and Lotus japonicus, which form indeterminate and...

Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid (2004)

Arai, Yuko, Shikanai, Toshiharu, Doi, Yoshiharu, Yoshida, Shigeo, Yamaguchi, Isamu, Nakashita, Hideo

Transgenic techniques are used to enhance and improve crop production, and their application to the production of chemical resources in plants has been under investigation. To achieve this latter...

Expression profiling of the developing and mature Nrl-/- mouse retina: identification of retinal disease candidates and transcriptional regulatory targets of Nrl (2004)

Yoshida, Shigeo, Mears, Alan J., Friedman, James S., Carter, Todd, He, Shirley, Oh, Edwin, ...

The rod photoreceptor-specific neural retina leucine zipper protein Nrl is essential for rod differentiation and plays a critical role in regulating gene expression. In the mouse retina, rods account...

Function Analysis of Phototropin2 using Fern Mutants Deficient in Blue Light-Induced Chloroplast Avoidance Movement (2004)

Kagawa, Takatoshi, Kasahara, Masahiro, Abe, Tomoko, Yoshida, Shigeo, Wada, Masamitsu

Gametophytes of the fern Adiantum capillus-veneris L. were mutagenized by heavy ion beam irradiation and screened for mutants lacking chloroplast avoidance movement under high intensity blue light....

Preference of DNA Methyltransferases for CpG Islands in Mouse Embryonic Stem Cells (2004)

Hattori, Naka, Abe, Tetsuya, Hattori, Naoko, Suzuki, Masako, Matsuyama, Tomoki, Yoshida, Shigeo, ...

Many CpG islands have tissue-dependent and differentially methylated regions (T-DMRs) in normal cells and tissues. To elucidate how DNA methyltransferases (Dnmts) participate in methylation of the...

Expression profiling of the developing and mature Nrl-/- mouse retina: Identification of retinal disease candidates and transcriptional regulatory targets of Nrl (2004)

Yoshida, Shigeo, Mears, Alan J., Friedman, James S., Carter, Todd, He, Shirley, Oh, Edwin, ...

The rod photoreceptor-specific neural retina leucine zipper protein Nrl is essential for rod differentiation and plays a critical role in regulating gene expression. In the mouse retina, rods account...

Analysis of Hydrogen Peroxide–independent Expression of the High-light–inducible ELIP2 Gene with the Aid of the ELIP2 Promoter–Luciferase Fusion¶ (2003)

Mitsuhiro Kimura, Katsushi Manabe, Tomoko Abe, Shigeo Yoshida, Minami Matsui, Yoshiharu Y. Yamamoto

Intense and excessive light triggers the evolution of reactive oxygen species in chloroplasts, and these have the potential to cause damage. However, plants are able to respond to light stress and...

Identification of Arabidopsis Genes Regulated by High Light–Stress Using cDNA Microarray¶ (2003)

Mitsuhiro Kimura, Yoshiharu Y. Yamamoto, Motoaki Seki, Tetsuya Sakurai, Masakazu Sato, Tomoko Abe, ...

In plants, excess light has the potential to damage the photosynthetic apparatus. The damage is caused in part by reactive oxygen species (ROS) generated by electrons leaking from the photosynthetic...

Activation of cell proliferation by brassinolide application in tobacco BY-2 cells: effects of brassinolide on cell multiplication, cell-cycle-related gene expression, and organellar DNA contents (2003)

Miyazawa, Yutaka, Nakajima, Naoko, Abe, Tomoko, Sakai, Atsushi, Fujioka, Shozo, Kawano, Shigeyuki, ...

Brassinosteroids (BRs) are steroidal phytohormones that are essential for many processes in plant growth and development, such as cell expansion, vascular differentiation, and responses to stress....

Amyloplast Formation in Cultured Tobacco BY-2 Cells Requires a High Cytokinin Content (2002)

Miyazawa, Yutaka, Kato, Hisashi, Muranaka, Toshiya, Yoshida, Shigeo

When cytokinin-autonomous tobacco BY-2 cell cultures are transferred into 2,4-dichlorophenoxyacetic acid (2,4-D)-deprived medium, amyloplast development is initiated. Using this in vitro...

Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2 (2002)

Miyazawa, Yutaka, Sakai, Atsushi, Matsunaga, Sachihiro, Asami, Tadao, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi, ...

In cultured Bright Yellow‐2 tobacco (Nicotiana tabacum) cells, the depletion of 2,4‐dichlorophenoxyacetic acid (2,4‐D) in the culture medium induces amyloplast development. This...

A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls (2002)

Kuroha, Takeshi, Kato, Hisashi, Asami, Tadao, Yoshida, Shigeo, Kamada, Hiroshi, Satoh, Shinobu

Shoot cultures of cucumber were used to analyse the roles of root‐derived substances in adventitious root formation on hypocotyl tissues. Xylem sap collected from the roots of squash had a...

Chloroisonicotinamide Derivative Induces a Broad Range of Disease Resistance in Rice and Tobacco (2002)

Nakashita, Hideo, Yasuda, Michiko, Nishioka, Masanori, Hasegawa, Satoru, Arai, Yuko, Uramoto, Masakazu, ...

Systemic acquired resistance (SAR) is a potent innate immunity system in plants that is effective against a broad range of pathogens. SAR in dicotyledonous plants such as tobacco and Arabidopsis has...

Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis (2001)

Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...

Brassinazole, an Inhibitor of Brassinosteroid Biosynthesis, Inhibits Development of Secondary Xylem in Cress Plants (Lepidium sativum) (2001)

Nagata, Noriko, Asami, Tadao, Yoshida, Shigeo

Brassinazole (Brz) is a specific brassinosteroid biosynthesis inhibitor. Cress plants (Lepidium sativum) grown in medium containing Brz exhibited a slight predominance of phloem differentiation at...

Regulation of Chloroplast Gene Expression is Affected in ali, a Novel Tobacco Albino Mutant (2001)

Bae, Chang-Hyu, Abe, Tomoko, Matsuyama, Tomoki, Fukunishi, Nobuhisa, Nagata, Noriko, Nakano, Takeshi, ...

An albino mutant designated ali was isolated from an M2population derived from a tobacco (Nicotiana tabacum L. ‘BY-4’) plant which had been irradiated with a14N-ion beam at an early...

Isolation and Initial Characterization of 132-Hydroxychlorophyll a Induced by Cyclohexanedione Derivatives in Tobacco Cell Suspension Cultures (2000)

Jing-Ming Wang, Tadao Asami, Noboru Murofushi, Shigeo Yoshida

This paper reports that a new photobleaching compound, 2-(2-chloro-5-propoxycarbonylphenyl)aminomethylidene-5,5-dimethyl-cyclohexane-1,3-dione (RWH-21), stimulates accumulation of...

Partial Characterization of a 17 kDa Acidic Protein, EFP, Induced by Thiocarbamate in the Early Flowering Phase in Asparagus Seedlings (1996)

Yeo, Dennis, Abe, Tomoko, Abe, Hiroshi, Sakurai, Akira, Takio, Koji, Dohmae, Naoshi, ...

Asparagus officinalis seedlings treated with either thiocarbamates or carbamates are induced to flower within 2-3 weeks of application. SDS-PAGE and 2-D PAGE resolutions of total soluble proteins of...

Differential Effects of Urea/Triazine-type and Phenol-type Photosystem II Inhibitors on Inactivation of the Electron Transport and Degradation of the D1 Protein during Photoinhibition (1996)

Nakajima, Yoshihiro, Yoshida, Shigeo, Ono, Taka-aki

The loss of PSII activity (water → silicomolybdate) and the degradation of the D1 protein during photoinhibitory illumination were studied in the presence of the various PSII inhibitors that bind...

Effects of Gibberellins and Prohexadione on the Activities of Oryzain and {alpha}-Amylase in Rice Seeds (1994)

Soh, Chang-Ho, Kamiya, Yuji, Yoshida, Shigeo, Yamane, Hisakazu, Takahashi, Nobutaka

Oryzains, cysteine proteinases of rice seeds, are induced by GA3 in germinating rice seeds [Abe et al. (1987) Agric. Biol. Chem. 51: 1509]. The effects of GA1, GA3, GA4, GA9, and GA20 on the...

Sympathetically induced myocardial ischaemia causes the heart to release plasma kinin (1987)

MATSUKI, TAKAYUKI, SHOJI, TETSURO, YOSHIDA, SHIGEO, KUDOH, YASUO, MOTOE, MASAOMI, INOUE, MEGUMI, ...

A recently developed highly sensitive radioimmunoassay method for detecting plasma kinin was used to re-evaluate the results of previous studies, in which plasma kinin had been measured with a...

Infarct size limitation by the xanthine oxidase inhibitor, allopurinol, in closed-chest dogs with small infarcts (1985)

AKIZUKI, SATOSHI, YOSHIDA, SHIGEO, CHAMBERS, DAVID E, EDDY, LYNNE J, PARMLEY, LOREN F, YELLON, DEREK M, ...

The present study was designed to evaluate the ability of allopurinol to limit infarct size following permanent coronary occlusion in the greyhound. Coronary occlusion was produced by injecting 2.5...

BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis

Neff, Michael M., Nguyen, Serena M., Malancharuvil, Elizabeth J., Fujioka, Shozo, Noguchi, Takahiro, Seto, Hideharu, ...

The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in the photoreceptor phytochrome B (phyB). The adult phenotype of bas1-D phyB-4 double mutants mimics that...

The Arabidopsis dwarf1 Mutant Is Defective in the Conversion of 24-Methylenecholesterol to Campesterol in Brassinosteroid Biosynthesis1

Choe, Sunghwa, Dilkes, Brian P., Gregory, Brian D., Ross, Amanda S., Yuan, Heng, Noguchi, Takahiro, ...

Since the isolation and characterization of dwarf1-1 (dwf1-1) from a T-DNA insertion mutant population, phenotypically similar mutants, including deetiolated2 (det2), constitutive photomorphogenesis...

Molecular Characterization of Photomixotrophic Tobacco Cells Resistant to Protoporphyrinogen Oxidase-Inhibiting Herbicides1

Watanabe, Naohide, Che, Fang-Sik, Iwano, Megumi, Takayama, Seiji, Nakano, Takeshi, Yoshida, Shigeo, ...

Peroxidizing herbicides inhibit protoporphyrinogen oxidase (Protox), the last enzyme of the common branch of the chlorophyll- and heme-synthesis pathways. There are two isoenzymes of Protox, one of...

An essential role of a TatC homologue of a ΔpH- dependent protein transporter in thylakoid membrane formation during chloroplast development in Arabidopsis thaliana

Motohashi, Reiko, Nagata, Noriko, Ito, Takuya, Takahashi, Seiji, Hobo, Tokunori, Yoshida, Shigeo, ...

At least three transport systems function in targeting nuclear-encoded chloroplast proteins to the chloroplast thylakoid membrane. One of these systems requires a thylakoid pH gradient and is named...

A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response1

Koka, Chala V., Cerny, R. Eric, Gardner, Randy G., Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...

The dumpy (dpy) mutant of tomato (Lycopersicon esculentum Mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. Application of brassinolide and castasterone rescued...

Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor1

Asami, Tadao, Min, Yong Ki, Nagata, Noriko, Yamagishi, Kazutoshi, Takatsuto, Suguru, Fujioka, Shozo, ...

Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by...

Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts1

Che, Fang-Sik, Watanabe, Naohide, Iwano, Megumi, Inokuchi, Hachiro, Takayama, Seiji, Yoshida, Shigeo, ...

Protoporphyrinogen oxidase (Protox) is the last common enzyme in the biosynthesis of chlorophylls and heme. In plants, there are two isoenzymes of Protox, one located in plastids and other in the...

Biosynthetic Pathways of Brassinolide in Arabidopsis1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Tax, Frans E., Yoshida, Shigeo, ...

Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading...

Arabidopsis det2 Is Defective in the Conversion of (24R)-24-Methylcholest-4-En-3-One to (24R)-24-Methyl-5α-Cholestan-3-One in Brassinosteroid Biosynthesis1

Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Sakurai, Akira, Yoshida, Shigeo, Li, Jianming, ...

Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5α-reductase) acts early in the proposed...

Brassinosteroid-Insensitive Dwarf Mutants of Arabidopsis Accumulate Brassinosteroids1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Yoshida, Shigeo, Yuan, Heng, ...

Seven dwarf mutants resembling brassinosteroid (BR)-biosynthetic dwarfs were isolated that did not respond significantly to the application of exogenous BRs. Genetic and molecular analyses revealed...

Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements

Yamamoto, Ryo, Fujioka, Shozo, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, Fukuda, Hiroo

As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary...

Involvement of a Nuclear-Encoded Basic Helix-Loop-Helix Protein in Transcription of the Light-Responsive Promoter of psbD1

Baba, Kyoko, Nakano, Takeshi, Yamagishi, Kazutoshi, Yoshida, Shigeo

In the chloroplast psbD light-responsive promoter (LRP), a highly conserved sequence exists upstream from the bacterial −10/−35 elements. Multiple sequence-specific DNA binding proteins are...

Brassinosteroid-6-Oxidases from Arabidopsis and Tomato Catalyze Multiple C-6 Oxidations in Brassinosteroid Biosynthesis1

Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...

A Chloroplast Protein Homologous to the Eubacterial Topological Specificity Factor MinE Plays a Role in Chloroplast Division1

Itoh, Ryuuichi, Fujiwara, Makoto, Nagata, Noriko, Yoshida, Shigeo

We report the identification of a nucleus-encoded minE gene, designated AtMinE1, of Arabidopsis. The encoded AtMinE1 protein possesses both N- and C-terminal extensions, relative to the eubacterial...

The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning

Carland, Francine M., Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Nelson, Timothy

Vascular cell axialization refers to the uniform alignment of vascular strands. In the Arabidopsis cotyledon vascular pattern1 (cvp1) mutant, vascular cells are not arranged in parallel files and are...

A Diterpene as an Endogenous Signal for the Activation of Defense Responses to Infection with Tobacco mosaic virus and Wounding in Tobacco

Seo, Shigemi, Seto, Hideharu, Koshino, Hiroyuki, Yoshida, Shigeo, Ohashi, Yuko

In pathogen-infected or wounded tobacco plants, the activation of wound-induced protein kinase (WIPK), a tobacco mitogen-activated protein kinase, has been implicated in the defense response....

Control of in vitro organogenesis by cyclin-dependent kinase activities in plants

Yamaguchi, Masatoshi, Kato, Hisashi, Yoshida, Shigeo, Yamamura, Saburo, Uchimiya, Hirofumi, Umeda, Masaaki

Little is known concerning the mechanisms by which auxin and cytokinin exert their effects on proliferation and differentiation. Cyclin-dependent kinases (CDKs) are major regulators of the eukaryotic...

An Early C-22 Oxidation Branch in the Brassinosteroid Biosynthetic Pathway

Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo

The natural occurrence of 22-hydroxylated steroids in cultured Catharanthus roseus cells and in Arabidopsis seedlings was investigated. Using full-scan gas chromatography-mass spectrometry analysis,...

Microarray Analysis of Brassinosteroid-Regulated Genes in Arabidopsis

Goda, Hideki, Shimada, Yukihisa, Asami, Tadao, Fujioka, Shozo, Yoshida, Shigeo

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. Although insights into the functions of BRs have been provided by recent studies of biosynthesis and...

Arabidopsis Brassinosteroid-Insensitive dwarf12 Mutants Are Semidominant and Defective in a Glycogen Synthase Kinase 3β-Like Kinase1

Choe, Sunghwa, Schmitz, Robert J., Fujioka, Shozo, Takatsuto, Suguru, Lee, Mi-Ok, Yoshida, Shigeo, ...

Mutants defective in the biosynthesis or signaling of brassinosteroids (BRs), plant steroid hormones, display dwarfism. Loss-of-function mutants for the gene encoding the plasma membrane-located BR...

Organ-Specific Expression of Brassinosteroid-Biosynthetic Genes and Distribution of Endogenous Brassinosteroids in Arabidopsis

Shimada, Yukihisa, Goda, Hideki, Nakamura, Ayako, Takatsuto, Suguru, Fujioka, Shozo, Yoshida, Shigeo

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. There is only limited information on where BRs are synthesized and used. We studied the organ...

Sterols Regulate Development and Gene Expression in Arabidopsis1

He, Jun-Xian, Fujioka, Shozo, Li, Tsai-Chi, Kang, Shin Gene, Seto, Hideharu, Takatsuto, Suguru, ...

Sterols are important not only for structural components of eukaryotic cell membranes but also for biosynthetic precursors of steroid hormones. In plants, the diverse functions of sterol-derived...

Global methylation screening in the Arabidopsis thaliana and Mus musculus genome: applications of virtual image restriction landmark genomic scanning (Vi-RLGS)

Matsuyama, Tomoki, Kimura, Makoto T., Koike, Kuniaki, Abe, Tomoko, Nakano, Takeshi, Asami, Tadao, ...

Understanding the role of ‘epigenetic’ changes such as DNA methylation and chromatin remodeling has now become critical in understanding many biological processes. In order to delineate the...

A Rice Brassinosteroid-Deficient Mutant, ebisu dwarf (d2), Is Caused by a Loss of Function of a New Member of Cytochrome P450

Hong, Zhi, Ueguchi-Tanaka, Miyako, Umemura, Kazuto, Uozu, Sakurako, Fujioka, Shozo, Takatsuto, Suguru, ...

We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of...

CYP72B1 Inactivates Brassinosteroid Hormones: An Intersection between Photomorphogenesis and Plant Steroid Signal Transduction1

Turk, Edward M., Fujioka, Shozo, Seto, Hideharu, Shimada, Yukihisa, Takatsuto, Suguru, Yoshida, Shigeo, ...

Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase encoded by CYP72B1 has been implicated in...

Brassinolide Induces IAA5, IAA19, and DR5, a Synthetic Auxin Response Element in Arabidopsis, Implying a Cross Talk Point of Brassinosteroid and Auxin Signaling

Nakamura, Ayako, Higuchi, Kanako, Goda, Hideki, Fujiwara, Makoto T., Sawa, Shinichiro, Koshiba, Tomokazu, ...

Despite numerous physiological studies addressing the interactions between brassinosteroids (BRs) and auxins, little is known about the underlying molecular mechanisms. We studied the expression of...

A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana

Jang, Jyan-Chyun, Fujioka, Shozo, Tasaka, Masao, Seto, Hideharu, Takatsuto, Suguru, Ishii, Akira, ...

Here we report a novel Arabidopsis dwarf mutant, fackel-J79, whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons,...

Comprehensive Comparison of Auxin-Regulated and Brassinosteroid-Regulated Genes in Arabidopsis[w]

Goda, Hideki, Sawa, Shinichiro, Asami, Tadao, Fujioka, Shozo, Shimada, Yukihisa, Yoshida, Shigeo

Although numerous physiological studies have addressed the interactions between brassinosteroids and auxins, little is known about the underlying molecular mechanisms. Using an Affymetrix GeneChip...

Preference of DNA Methyltransferases for CpG Islands in Mouse Embryonic Stem Cells

Hattori, Naka, Abe, Tetsuya, Hattori, Naoko, Suzuki, Masako, Matsuyama, Tomoki, Yoshida, Shigeo, ...

Many CpG islands have tissue-dependent and differentially methylated regions (T-DMRs) in normal cells and tissues. To elucidate how DNA methyltransferases (Dnmts) participate in methylation of the...

A Novel Inhibitor of 9-cis-Epoxycarotenoid Dioxygenase in Abscisic Acid Biosynthesis in Higher Plants1

Han, Sun-Young, Kitahata, Nobutaka, Sekimata, Katsuhiko, Saito, Tamio, Kobayashi, Masatomo, Nakashima, Kazuo, ...

Abscisic acid (ABA) is a major regulator in the adaptation of plants to environmental stresses, plant growth, and development. In higher plants, the ABA biosynthesis pathway involves the oxidative...

A Novel Cytochrome P450 Is Implicated in Brassinosteroid Biosynthesis via the Characterization of a Rice Dwarf Mutant, dwarf11, with Reduced Seed Length

Tanabe, Sumiyo, Ashikari, Motoyuki, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Yano, Masahiro, ...

We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, the D11 gene was isolated...

Specific Binding of Protoporphyrin IX to a Membrane-Bound 63 Kilodalton Polypeptide in Cucumber Cotyledons Treated with Diphenyl Ether-Type Herbicides

Sato, Ryo, Oshio, Hiromichi, Koike, Hiroyuki, Inoue, Yorinao, Yoshida, Shigeo, Takahashi, Nobutaka

Porphyrin accumulation in excised cucumber cotyledons (Cucumis sativus L.) treated with a N-phenylimide S-23142 (N-[4-chloro-2-fluoro-5-propargyloxyphenyl]-3,4,5,6- tetrahydrophthalimide) and a...

Mechanisms of aging in senescence-accelerated mice

Carter, Todd A, Greenhall, Jennifer A, Yoshida, Shigeo, Fuchs, Sebastian, Helton, Robert, Swaroop, Anand, ...

Gene-expression analysis and polymorphism screening to study molecular senescence of the retina and hippocampus in two rare inbred mouse models of accelerated neurological senescence as well as a...

Triadimefon, a fungicidal triazole-type P450 inhibitor, induces brassinosteroid deficiency-like phenotypes in plants and binds to DWF4 protein in the brassinosteroid biosynthesis pathway.

Asami, Tadao, Mizutani, Masaharu, Shimada, Yukihisa, Goda, Hideki, Kitahata, Nobutaka, Sekimata, Katsuhiko, ...

Triadimefon (Bayleton), a widely used triazole-type fungicide, affects gibberellin (GA) biosynthesis and 14 alpha-demethylase in sterol biosynthesis. The present study revealed that the phenotype of...

The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids

Poppenberger, Brigitte, Fujioka, Shozo, Soeno, Kazuo, George, Gilu L., Vaistij, Fabián E., Hiranuma, Sayoko, ...

Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important...

Identification and Functional Analysis of in Vivo Phosphorylation Sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 Receptor Kinase

Wang, Xiaofeng, Goshe, Michael B., Soderblom, Erik J., Phinney, Brett S., Kuchar, Jason A., Li, Jia, ...

Brassinosteroids (BRs) regulate multiple aspects of plant growth and development and require an active BRASSINOSTEROID-INSENSITIVE1 (BRI1) and BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) for hormone...

Brassinosteroid Homeostasis in Arabidopsis Is Ensured by Feedback Expressions of Multiple Genes Involved in Its Metabolism

Tanaka, Kiwamu, Asami, Tadao, Yoshida, Shigeo, Nakamura, Yasushi, Matsuo, Tomoaki, Okamoto, Shigehisa

Homeostasis of brassinosteroids (BRs) is essential for normal growth and development in higher plants. We examined responsiveness of 11 BR metabolic gene expressions to the decrease or increase of...

The Rice brassinosteroid-deficient dwarf2 Mutant, Defective in the Rice Homolog of Arabidopsis DIMINUTO/DWARF1, Is Rescued by the Endogenously Accumulated Alternative Bioactive Brassinosteroid, Dolichosterone

Hong, Zhi, Ueguchi-Tanaka, Miyako, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Hasegawa, Yasuko, ...

We have identified a rice (Oryza sativa) brassinosteroid (BR)-deficient mutant, BR-deficient dwarf2 (brd2). The brd2 locus contains a single base deletion in the coding region of Dim/dwf1, a homolog...

TANMEI/EMB2757 Encodes a WD Repeat Protein Required for Embryo Development in Arabidopsis1

Yamagishi, Kazutoshi, Nagata, Noriko, Yee, Kelly Matsudaira, Braybrook, Siobhan A., Pelletier, Julie, Fujioka, Shozo, ...

We identified the Arabidopsis (Arabidopsis thaliana) tanmei/emb2757 (tan) mutation that causes defects in both embryo and seedling development. tan mutant embryos share many characteristics with the...

BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis

Neff, Michael M., Nguyen, Serena M., Malancharuvil, Elizabeth J., Fujioka, Shozo, Noguchi, Takahiro, Seto, Hideharu, ...

The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in the photoreceptor phytochrome B (phyB). The adult phenotype of bas1-D phyB-4 double mutants mimics that...

The Arabidopsis dwarf1 Mutant Is Defective in the Conversion of 24-Methylenecholesterol to Campesterol in Brassinosteroid Biosynthesis1

Choe, Sunghwa, Dilkes, Brian P., Gregory, Brian D., Ross, Amanda S., Yuan, Heng, Noguchi, Takahiro, ...

Since the isolation and characterization of dwarf1-1 (dwf1-1) from a T-DNA insertion mutant population, phenotypically similar mutants, including deetiolated2 (det2), constitutive photomorphogenesis...

Molecular Characterization of Photomixotrophic Tobacco Cells Resistant to Protoporphyrinogen Oxidase-Inhibiting Herbicides1

Watanabe, Naohide, Che, Fang-Sik, Iwano, Megumi, Takayama, Seiji, Nakano, Takeshi, Yoshida, Shigeo, ...

Peroxidizing herbicides inhibit protoporphyrinogen oxidase (Protox), the last enzyme of the common branch of the chlorophyll- and heme-synthesis pathways. There are two isoenzymes of Protox, one of...

An essential role of a TatC homologue of a ΔpH- dependent protein transporter in thylakoid membrane formation during chloroplast development in Arabidopsis thaliana

Motohashi, Reiko, Nagata, Noriko, Ito, Takuya, Takahashi, Seiji, Hobo, Tokunori, Yoshida, Shigeo, ...

At least three transport systems function in targeting nuclear-encoded chloroplast proteins to the chloroplast thylakoid membrane. One of these systems requires a thylakoid pH gradient and is named...

A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response1

Koka, Chala V., Cerny, R. Eric, Gardner, Randy G., Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...

The dumpy (dpy) mutant of tomato (Lycopersicon esculentum Mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. Application of brassinolide and castasterone rescued...

Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor1

Asami, Tadao, Min, Yong Ki, Nagata, Noriko, Yamagishi, Kazutoshi, Takatsuto, Suguru, Fujioka, Shozo, ...

Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by...

Molecular Characterization and Subcellular Localization of Protoporphyrinogen Oxidase in Spinach Chloroplasts1

Che, Fang-Sik, Watanabe, Naohide, Iwano, Megumi, Inokuchi, Hachiro, Takayama, Seiji, Yoshida, Shigeo, ...

Protoporphyrinogen oxidase (Protox) is the last common enzyme in the biosynthesis of chlorophylls and heme. In plants, there are two isoenzymes of Protox, one located in plastids and other in the...

Biosynthetic Pathways of Brassinolide in Arabidopsis1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Tax, Frans E., Yoshida, Shigeo, ...

Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading...

Arabidopsis det2 Is Defective in the Conversion of (24R)-24-Methylcholest-4-En-3-One to (24R)-24-Methyl-5α-Cholestan-3-One in Brassinosteroid Biosynthesis1

Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Sakurai, Akira, Yoshida, Shigeo, Li, Jianming, ...

Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5α-reductase) acts early in the proposed...

Brassinosteroid-Insensitive Dwarf Mutants of Arabidopsis Accumulate Brassinosteroids1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Yoshida, Shigeo, Yuan, Heng, ...

Seven dwarf mutants resembling brassinosteroid (BR)-biosynthetic dwarfs were isolated that did not respond significantly to the application of exogenous BRs. Genetic and molecular analyses revealed...

Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements

Yamamoto, Ryo, Fujioka, Shozo, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, Fukuda, Hiroo

As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary...

Involvement of a Nuclear-Encoded Basic Helix-Loop-Helix Protein in Transcription of the Light-Responsive Promoter of psbD1

Baba, Kyoko, Nakano, Takeshi, Yamagishi, Kazutoshi, Yoshida, Shigeo

In the chloroplast psbD light-responsive promoter (LRP), a highly conserved sequence exists upstream from the bacterial −10/−35 elements. Multiple sequence-specific DNA binding proteins are...

Brassinosteroid-6-Oxidases from Arabidopsis and Tomato Catalyze Multiple C-6 Oxidations in Brassinosteroid Biosynthesis1

Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...

A Chloroplast Protein Homologous to the Eubacterial Topological Specificity Factor MinE Plays a Role in Chloroplast Division1

Itoh, Ryuuichi, Fujiwara, Makoto, Nagata, Noriko, Yoshida, Shigeo

We report the identification of a nucleus-encoded minE gene, designated AtMinE1, of Arabidopsis. The encoded AtMinE1 protein possesses both N- and C-terminal extensions, relative to the eubacterial...

The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning

Carland, Francine M., Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Nelson, Timothy

Vascular cell axialization refers to the uniform alignment of vascular strands. In the Arabidopsis cotyledon vascular pattern1 (cvp1) mutant, vascular cells are not arranged in parallel files and are...

A Diterpene as an Endogenous Signal for the Activation of Defense Responses to Infection with Tobacco mosaic virus and Wounding in Tobacco

Seo, Shigemi, Seto, Hideharu, Koshino, Hiroyuki, Yoshida, Shigeo, Ohashi, Yuko

In pathogen-infected or wounded tobacco plants, the activation of wound-induced protein kinase (WIPK), a tobacco mitogen-activated protein kinase, has been implicated in the defense response....

Control of in vitro organogenesis by cyclin-dependent kinase activities in plants

Yamaguchi, Masatoshi, Kato, Hisashi, Yoshida, Shigeo, Yamamura, Saburo, Uchimiya, Hirofumi, Umeda, Masaaki

Little is known concerning the mechanisms by which auxin and cytokinin exert their effects on proliferation and differentiation. Cyclin-dependent kinases (CDKs) are major regulators of the eukaryotic...

An Early C-22 Oxidation Branch in the Brassinosteroid Biosynthetic Pathway

Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo

The natural occurrence of 22-hydroxylated steroids in cultured Catharanthus roseus cells and in Arabidopsis seedlings was investigated. Using full-scan gas chromatography-mass spectrometry analysis,...

Microarray Analysis of Brassinosteroid-Regulated Genes in Arabidopsis

Goda, Hideki, Shimada, Yukihisa, Asami, Tadao, Fujioka, Shozo, Yoshida, Shigeo

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. Although insights into the functions of BRs have been provided by recent studies of biosynthesis and...

Arabidopsis Brassinosteroid-Insensitive dwarf12 Mutants Are Semidominant and Defective in a Glycogen Synthase Kinase 3β-Like Kinase1

Choe, Sunghwa, Schmitz, Robert J., Fujioka, Shozo, Takatsuto, Suguru, Lee, Mi-Ok, Yoshida, Shigeo, ...

Mutants defective in the biosynthesis or signaling of brassinosteroids (BRs), plant steroid hormones, display dwarfism. Loss-of-function mutants for the gene encoding the plasma membrane-located BR...

Organ-Specific Expression of Brassinosteroid-Biosynthetic Genes and Distribution of Endogenous Brassinosteroids in Arabidopsis

Shimada, Yukihisa, Goda, Hideki, Nakamura, Ayako, Takatsuto, Suguru, Fujioka, Shozo, Yoshida, Shigeo

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. There is only limited information on where BRs are synthesized and used. We studied the organ...

Sterols Regulate Development and Gene Expression in Arabidopsis1

He, Jun-Xian, Fujioka, Shozo, Li, Tsai-Chi, Kang, Shin Gene, Seto, Hideharu, Takatsuto, Suguru, ...

Sterols are important not only for structural components of eukaryotic cell membranes but also for biosynthetic precursors of steroid hormones. In plants, the diverse functions of sterol-derived...

Global methylation screening in the Arabidopsis thaliana and Mus musculus genome: applications of virtual image restriction landmark genomic scanning (Vi-RLGS)

Matsuyama, Tomoki, Kimura, Makoto T., Koike, Kuniaki, Abe, Tomoko, Nakano, Takeshi, Asami, Tadao, ...

Understanding the role of ‘epigenetic’ changes such as DNA methylation and chromatin remodeling has now become critical in understanding many biological processes. In order to delineate the...

A Rice Brassinosteroid-Deficient Mutant, ebisu dwarf (d2), Is Caused by a Loss of Function of a New Member of Cytochrome P450

Hong, Zhi, Ueguchi-Tanaka, Miyako, Umemura, Kazuto, Uozu, Sakurako, Fujioka, Shozo, Takatsuto, Suguru, ...

We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of...

CYP72B1 Inactivates Brassinosteroid Hormones: An Intersection between Photomorphogenesis and Plant Steroid Signal Transduction1

Turk, Edward M., Fujioka, Shozo, Seto, Hideharu, Shimada, Yukihisa, Takatsuto, Suguru, Yoshida, Shigeo, ...

Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase encoded by CYP72B1 has been implicated in...

Brassinolide Induces IAA5, IAA19, and DR5, a Synthetic Auxin Response Element in Arabidopsis, Implying a Cross Talk Point of Brassinosteroid and Auxin Signaling

Nakamura, Ayako, Higuchi, Kanako, Goda, Hideki, Fujiwara, Makoto T., Sawa, Shinichiro, Koshiba, Tomokazu, ...

Despite numerous physiological studies addressing the interactions between brassinosteroids (BRs) and auxins, little is known about the underlying molecular mechanisms. We studied the expression of...

A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana

Jang, Jyan-Chyun, Fujioka, Shozo, Tasaka, Masao, Seto, Hideharu, Takatsuto, Suguru, Ishii, Akira, ...

Here we report a novel Arabidopsis dwarf mutant, fackel-J79, whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons,...

Comprehensive Comparison of Auxin-Regulated and Brassinosteroid-Regulated Genes in Arabidopsis[w]

Goda, Hideki, Sawa, Shinichiro, Asami, Tadao, Fujioka, Shozo, Shimada, Yukihisa, Yoshida, Shigeo

Although numerous physiological studies have addressed the interactions between brassinosteroids and auxins, little is known about the underlying molecular mechanisms. Using an Affymetrix GeneChip...

Preference of DNA Methyltransferases for CpG Islands in Mouse Embryonic Stem Cells

Hattori, Naka, Abe, Tetsuya, Hattori, Naoko, Suzuki, Masako, Matsuyama, Tomoki, Yoshida, Shigeo, ...

Many CpG islands have tissue-dependent and differentially methylated regions (T-DMRs) in normal cells and tissues. To elucidate how DNA methyltransferases (Dnmts) participate in methylation of the...

A Novel Inhibitor of 9-cis-Epoxycarotenoid Dioxygenase in Abscisic Acid Biosynthesis in Higher Plants1

Han, Sun-Young, Kitahata, Nobutaka, Sekimata, Katsuhiko, Saito, Tamio, Kobayashi, Masatomo, Nakashima, Kazuo, ...

Abscisic acid (ABA) is a major regulator in the adaptation of plants to environmental stresses, plant growth, and development. In higher plants, the ABA biosynthesis pathway involves the oxidative...

A Novel Cytochrome P450 Is Implicated in Brassinosteroid Biosynthesis via the Characterization of a Rice Dwarf Mutant, dwarf11, with Reduced Seed Length

Tanabe, Sumiyo, Ashikari, Motoyuki, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Yano, Masahiro, ...

We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, the D11 gene was isolated...

Specific Binding of Protoporphyrin IX to a Membrane-Bound 63 Kilodalton Polypeptide in Cucumber Cotyledons Treated with Diphenyl Ether-Type Herbicides

Sato, Ryo, Oshio, Hiromichi, Koike, Hiroyuki, Inoue, Yorinao, Yoshida, Shigeo, Takahashi, Nobutaka

Porphyrin accumulation in excised cucumber cotyledons (Cucumis sativus L.) treated with a N-phenylimide S-23142 (N-[4-chloro-2-fluoro-5-propargyloxyphenyl]-3,4,5,6- tetrahydrophthalimide) and a...

Identification and Functional Analysis of in Vivo Phosphorylation Sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 Receptor Kinase

Wang, Xiaofeng, Goshe, Michael B., Soderblom, Erik J., Phinney, Brett S., Kuchar, Jason A., Li, Jia, ...

Brassinosteroids (BRs) regulate multiple aspects of plant growth and development and require an active BRASSINOSTEROID-INSENSITIVE1 (BRI1) and BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) for hormone...

Brassinosteroid Homeostasis in Arabidopsis Is Ensured by Feedback Expressions of Multiple Genes Involved in Its Metabolism

Tanaka, Kiwamu, Asami, Tadao, Yoshida, Shigeo, Nakamura, Yasushi, Matsuo, Tomoaki, Okamoto, Shigehisa

Homeostasis of brassinosteroids (BRs) is essential for normal growth and development in higher plants. We examined responsiveness of 11 BR metabolic gene expressions to the decrease or increase of...

Mechanisms of aging in senescence-accelerated mice

Carter, Todd A, Greenhall, Jennifer A, Yoshida, Shigeo, Fuchs, Sebastian, Helton, Robert, Swaroop, Anand, ...

Gene-expression analysis and polymorphism screening to study molecular senescence of the retina and hippocampus in two rare inbred mouse models of accelerated neurological senescence as well as a...

Age-Related Macular Degeneration: A High-Resolution Genome Scan for Susceptibility Loci in a Population Enriched for Late-Stage Disease

Abecasis, Gonçalo R., Yashar, Beverly M., Zhao, Yu, Ghiasvand, Noor M., Zareparsi, Sepideh, Branham, Kari E. H., ...

Age-related macular degeneration (AMD) is a complex multifactorial disease that affects the central region of the retina. AMD is clinically heterogeneous, leading to geographic atrophy (GA) and/or...

The Rice brassinosteroid-deficient dwarf2 Mutant, Defective in the Rice Homolog of Arabidopsis DIMINUTO/DWARF1, Is Rescued by the Endogenously Accumulated Alternative Bioactive Brassinosteroid, Dolichosterone

Hong, Zhi, Ueguchi-Tanaka, Miyako, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Hasegawa, Yasuko, ...

We have identified a rice (Oryza sativa) brassinosteroid (BR)-deficient mutant, BR-deficient dwarf2 (brd2). The brd2 locus contains a single base deletion in the coding region of Dim/dwf1, a homolog...

TANMEI/EMB2757 Encodes a WD Repeat Protein Required for Embryo Development in Arabidopsis1

Yamagishi, Kazutoshi, Nagata, Noriko, Yee, Kelly Matsudaira, Braybrook, Siobhan A., Pelletier, Julie, Fujioka, Shozo, ...

We identified the Arabidopsis (Arabidopsis thaliana) tanmei/emb2757 (tan) mutation that causes defects in both embryo and seedling development. tan mutant embryos share many characteristics with the...

Triadimefon, a fungicidal triazole-type P450 inhibitor, induces brassinosteroid deficiency-like phenotypes in plants and binds to DWF4 protein in the brassinosteroid biosynthesis pathway.

Asami, Tadao, Mizutani, Masaharu, Shimada, Yukihisa, Goda, Hideki, Kitahata, Nobutaka, Sekimata, Katsuhiko, ...

Triadimefon (Bayleton), a widely used triazole-type fungicide, affects gibberellin (GA) biosynthesis and 14 alpha-demethylase in sterol biosynthesis. The present study revealed that the phenotype of...

The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids

Poppenberger, Brigitte, Fujioka, Shozo, Soeno, Kazuo, George, Gilu L., Vaistij, Fabián E., Hiranuma, Sayoko, ...

Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important...

The Regulation of DWARF4 Expression Is Likely a Critical Mechanism in Maintaining the Homeostasis of Bioactive Brassinosteroids in Arabidopsis1

Kim, Ho Bang, Kwon, Mi, Ryu, Hojin, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, ...

Mutants that are defective in brassinosteroid (BR) biosynthesis or signaling display severely retarded growth patterns due to absence of growth-promoting effects by BRs. Arabidopsis (Arabidopsis...

The Role of OsBRI1 and Its Homologous Genes, OsBRL1 and OsBRL3, in Rice1

Nakamura, Ayako, Fujioka, Shozo, Sunohara, Hidehiko, Kamiya, Noriko, Hong, Zhi, Inukai, Yoshiaki, ...

Since first identifying two alleles of a rice (Oryza sativa) brassinosteroid (BR)-insensitive mutant, d61, that were also defective in an orthologous gene in Arabidopsis (Arabidopsis thaliana)...

Antagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in Arabidopsis[W]

Yasuda, Michiko, Ishikawa, Atsushi, Jikumaru, Yusuke, Seki, Motoaki, Umezawa, Taishi, Asami, Tadao, ...

Systemic acquired resistance (SAR) is a potent innate immunity system in plants that is effective against a broad range of pathogens. SAR development in dicotyledonous plants, such as tobacco...