Shihui Liu

Identification of a Receptor-Binding Region within Domain 4 of the Protective Antigen Component of Anthrax Toxin

Varughese, Mini, Teixeira, Avelino V., Liu, Shihui, Leppla, Stephen H.

Anthrax toxin from Bacillus anthracis is a three-component toxin consisting of lethal factor (LF), edema factor (EF), and protective antigen (PA). LF and EF are the catalytic components of the toxin,...

Potent antitumor activity of a urokinase-activated engineered anthrax toxin

Liu, Shihui, Aaronson, Hannah, Mitola, David J., Leppla, Stephen H., Bugge, Thomas H.

The acquisition of cell-surface urokinase plasminogen activator activity is a hallmark of malignancy. We generated an engineered anthrax toxin that is activated by cell-surface urokinase in vivo and...

Identification of the Proteins Required for Biosynthesis of Diphthamide, the Target of Bacterial ADP-Ribosylating Toxins on Translation Elongation Factor 2†

Liu, Shihui, Milne, G. Todd, Kuremsky, Jeffrey G., Fink, Gerald R., Leppla, Stephen H.

Diphthamide, a posttranslational modification of translation elongation factor 2 that is conserved in all eukaryotes and archaebacteria and is the target of diphtheria toxin, is formed in yeast by...

Dph3, a Small Protein Required for Diphthamide Biosynthesis, Is Essential in Mouse Development

Liu, Shihui, Wiggins, Jason F., Sreenath, Taduru, Kulkarni, Ashok B., Ward, Jerrold M., Leppla, Stephen H.

The translation elongation factor 2 in eukaryotes (eEF-2) contains a unique posttranslationally modified histidine residue, termed diphthamide, which serves as the only target for diphtheria toxin...

Identification of a Receptor-Binding Region within Domain 4 of the Protective Antigen Component of Anthrax Toxin

Varughese, Mini, Teixeira, Avelino V., Liu, Shihui, Leppla, Stephen H.

Anthrax toxin from Bacillus anthracis is a three-component toxin consisting of lethal factor (LF), edema factor (EF), and protective antigen (PA). LF and EF are the catalytic components of the toxin,...

Potent antitumor activity of a urokinase-activated engineered anthrax toxin

Liu, Shihui, Aaronson, Hannah, Mitola, David J., Leppla, Stephen H., Bugge, Thomas H.

The acquisition of cell-surface urokinase plasminogen activator activity is a hallmark of malignancy. We generated an engineered anthrax toxin that is activated by cell-surface urokinase in vivo and...

Identification of the Proteins Required for Biosynthesis of Diphthamide, the Target of Bacterial ADP-Ribosylating Toxins on Translation Elongation Factor 2†

Liu, Shihui, Milne, G. Todd, Kuremsky, Jeffrey G., Fink, Gerald R., Leppla, Stephen H.

Diphthamide, a posttranslational modification of translation elongation factor 2 that is conserved in all eukaryotes and archaebacteria and is the target of diphtheria toxin, is formed in yeast by...

Dph3, a Small Protein Required for Diphthamide Biosynthesis, Is Essential in Mouse Development

Liu, Shihui, Wiggins, Jason F., Sreenath, Taduru, Kulkarni, Ashok B., Ward, Jerrold M., Leppla, Stephen H.

The translation elongation factor 2 in eukaryotes (eEF-2) contains a unique posttranslationally modified histidine residue, termed diphthamide, which serves as the only target for diphtheria toxin...

Anthrax toxin triggers endocytosis of its receptor via a lipid raft–mediated clathrin-dependent process

Abrami, Laurence, Liu, Shihui, Cosson, Pierre, Leppla, Stephen H., Van Der Goot, F. Gisou

The protective antigen (PA) of the anthrax toxin binds to a cell surface receptor and thereby allows lethal factor (LF) to be taken up and exert its toxic effect in the cytoplasm. Here, we report...