Noriko Nagahori, Midori Abe, Shin-Ichiro Nishimura
Glycosphingolipids (GSLs) synthesized in Golgi apparatus by sequential transfer of sugar residues to a ceramide lipid anchor are ubiquitously distributing on vertebrate plasma membranes. Standardized...
Detection of Carcinoembryonic Antigens Using a Surface Plasmon Resonance Biosensor (2008)
Fengyu Su, Chunye Xu, Minoru Taya, Kimie Murayama, Yasuro Shinohara, Shin-Ichiro Nishimura
Carcinoembryonic antigen (CEA) is an oncofoetal cell-surface glycoprotein that serves as an important tumor marker for colorectal and some other carcinomas. In this work, a CEA immunoassay using a...
Nakahara, Taku, Hashimoto, Ryo, Nakagawa, Hiroaki, Monde, Kenji, Miura, Nobuaki, Nishimura, Shin-Ichiro
Glycobiology has been brought to public attention as a frontier in the post-genomic era. Structural information about glycans has been accumulating in the Protein Data Bank (PDB) for years. It has...
Hamaguchi, Jun, Nakagawa, Hiroaki, Takahashi, Masato, Kudo, Takeaki, Kamiyama, Naoya, Sun, Bailong, ...
Abstract Background Drug resistance is a major problem in cancer chemotherapy. Acquisition of chemo-resistance not only reduces the effectiveness of drugs, but also promotes side effects and markedly...
Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients (2007)
Nakagawa, Hiroaki, Hato, Megumi, Takegawa, Yasuhiro, Deguchi, Kisaburo, Ito, Hiroki, Takahata, Masahiko, ...
Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have...
Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients. (2007)
Nakagawa, Hiroaki, Hato, Megumi, Takegawa, Yasuhiro, Deguchi, Kisaburo, Ito, Hiroki, Takahata, Masahiko, ...
Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have...
N-glycan alterations are associated with drug resistance in human hepatocellular carcinoma (2007)
Kudo, Takeaki, Nakagawa, Hiroaki, Takahashi, Masato, Hamaguchi, Jun, Kamiyama, Naoya, Yokoo, Hideki, ...
Abstract Background Correlations of disease phenotypes with glycosylation changes have been analysed intensively in the tumor biology field. Glycoforms potentially associated with carcinogenesis,...
N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin (2007)
Ito, Hiroko, Akiyama, Masashi, Nakagawa, Hiroaki, Uematsu, Rie, Deguchi, Kisaburo, McMillan, James R., ...
N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in...
N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin. (2007)
Ito, Hiroko, Akiyama, Masashi, Nakagawa, Hiroaki, Uematsu, Rie, Deguchi, Kisaburo, McMillan, James R., ...
N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in...
N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin. (2007)
Ito, Hiroko, Akiyama, Masashi, Nakagawa, Hiroaki, Uematsu, Rie, Deguchi, Kisaburo, McMillan, James R., ...
N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in...
Kosaka, Akemi, Wakita, Daiko, Matsubara, Naoki, Togashi, Yuji, Nishimura, Shin-Ichiro, Kitamura, Hidemitsu, ...
In unimmunized specific pathogen-free mice, there are unique memory-type CD8+ T cell populations expressing asialoGM1 (ASGM1). These cells were classified into central memory-type T cells (TCMT)...
Kosaka, Akemi, Wakita, Daiko, Matsubara, Naoki, Togashi, Yuji, Nishimura, Shin-Ichiro, Kitamura, Hidemitsu, ...
In unimmunized specific pathogen-free mice, there are unique memory-type CD8+ T cell populations expressing asialoGM1 (ASGM1). These cells were classified into central memory-type T cells (TCMT)...
Kosaka, Akemi, Wakita, Daiko, Matsubara, Naoki, Togashi, Yuji, Nishimura, Shin-Ichiro, Kitamura, Hidemitsu, ...
In unimmunized specific pathogen-free mice, there are unique memory-type CD8+ T cell populations expressing asialoGM1 (ASGM1). These cells were classified into central memory-type T cells (TCMT)...
Kato, Yasuhiro, Mawatari, Hideo, Nishimura, Shin-Ichiro, Sakura, Nobuo, Ueda, Kazuhiro
Several specific assays have been developed for the measurement of etoposide in biological fluids. As large samples are required for high sensitivity, these systems are not appropriate for a...
Takegawa, Yasuhiro, Deguchi, Kisaburo, Keira, Takuro, Ito, Hiroki, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro, ...
Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction...
Takegawa, Yasuhiro, Deguchi, Kisaburo, Keira, Takuro, Ito, Hiroki, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro
Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction...
Takegawa, Yasuhiro, Deguchi, Kisaburo, Keira, Takuro, Ito, Hiroki, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro
Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction...
Deguchi, Kisaburo, Takegawa, Yasuhiro, Ito, Hiroki, Miura, Nobuaki, Yoshioka, Shinji, Nagai, Shinji, ...
To investigate the possibility of structural assignment based on negative-ion MSn spectral matching, four isomers of 2-aminopyridine-derivatized (PA) monosialylated oligosaccharides (i.e., complex...
Deguchi, Kisaburo, Ito, Hiroki, Takegawa, Yasuhiro, Shinji, Nagai, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro, ...
Positive- and negative-ion MSn spectra of chicken egg yolk glycopeptides binding a neutral and a sialylated N-glycan were acquired by using electrospray ionization linear ion trap time-of-flight mass...
Deguchi, Kisaburo, Ito, Hiroki, Takegawa, Yasuhiro, Shinji, Nagai, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro
Positive- and negative-ion MSn spectra of chicken egg yolk glycopeptides binding a neutral and a sialylated N-glycan were acquired by using electrospray ionization linear ion trap time-of-flight mass...
Deguchi, Kisaburo, Takegawa, Yasuhiro, Ito, Hiroki, Miura, Nobuaki, Yoshioka, Shinji, Nagai, Shinji, ...
To investigate the possibility of structural assignment based on negative-ion MSn spectral matching, four isomers of 2-aminopyridine-derivatized (PA) monosialylated oligosaccharides (i.e., complex...
Ito, Hiroki, Takegawa, Yasuhiro, Deguchi, Kisaburo, Nagai, Shinji, Nakagawa, Hiroaki, Shinohara, Yasuro, ...
Mass spectrometric analyses of various N-glycans binding to proteins and peptides are highly desirable for elucidating their biological roles. An approach based on collision-induced dissociation...
Ito, Hiroki, Takegawa, Yasuhiro, Deguchi, Kisaburo, Nagai, Shinji, Nakagawa, Hiroaki, Shinohara, Yasuro, ...
Mass spectrometric analyses of various N-glycans binding to proteins and peptides are highly desirable for elucidating their biological roles. An approach based on collision-induced dissociation...
Deguchi, Kisaburo, Ito, Hiroki, Takegawa, Yasuhiro, Shinji, Nagai, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro
Positive- and negative-ion MSn spectra of chicken egg yolk glycopeptides binding a neutral and a sialylated N-glycan were acquired by using electrospray ionization linear ion trap time-of-flight mass...
Deguchi, Kisaburo, Takegawa, Yasuhiro, Ito, Hiroki, Miura, Nobuaki, Yoshioka, Shinji, Nagai, Shinji, ...
To investigate the possibility of structural assignment based on negative-ion MSn spectral matching, four isomers of 2-aminopyridine-derivatized (PA) monosialylated oligosaccharides (i.e., complex...
Ito, Hiroki, Takegawa, Yasuhiro, Deguchi, Kisaburo, Nagai, Shinji, Nakagawa, Hiroaki, Shinohara, Yasuro, ...
Mass spectrometric analyses of various N-glycans binding to proteins and peptides are highly desirable for elucidating their biological roles. An approach based on collision-induced dissociation...
Nakahara, Taku, Hindsgaul, Ole, Palcic, Monica M., Nishimura, Shin-Ichiro
Glycosyltransferases are an enormous and diverse class of enzyme encompassing 1% of all sequenced genomes. They catalyze the transfer of a monosaccharide from an activated donor such as a...
Uematsu, Rie, Furukawa, Jun-ichi, Nakagawa, Hiroaki, Shinohara, Yasuro, Deguchi, Kisaburo, Monde, Kenji, ...
Despite recent advances in our understanding of the significance of the protein glycosylation, the throughput of protein glycosylation analysis is still too low to be applied to the exhaustive...
Uematsu, Rie, Furukawa, Jun-ichi, Nakagawa, Hiroaki, Shinohara, Yasuro, Deguchi, Kisaburo, Monde, Kenji, ...
Despite recent advances in our understanding of the significance of the protein glycosylation, the throughput of protein glycosylation analysis is still too low to be applied to the exhaustive...
Uematsu, Rie, Furukawa, Jun-ichi, Nakagawa, Hiroaki, Shinohara, Yasuro, Deguchi, Kisaburo, Monde, Kenji, ...
Despite recent advances in our understanding of the significance of the protein glycosylation, the throughput of protein glycosylation analysis is still too low to be applied to the exhaustive...
Akama, Tomoya O., Nakagawa, Hiroaki, Wong, Nyet Kui, Sutton-Smith, Mark, Dell, Anne, Morris, Howard R., ...
Many proteins synthesized through the secretory pathway receive posttranslational modifications, including N-glycosylation. α-Mannosidase II (MII) is a key enzyme converting precursor...
Akama, Tomoya O., Nakagawa, Hiroaki, Wong, Nyet Kui, Sutton-Smith, Mark, Dell, Anne, Morris, Howard R., ...
Many proteins synthesized through the secretory pathway receive posttranslational modifications, including N-glycosylation. α-Mannosidase II (MII) is a key enzyme converting precursor...
N-glycan alterations are associated with drug resistance in human hepatocellular carcinoma
Kudo, Takeaki, Nakagawa, Hiroaki, Takahashi, Masato, Hamaguchi, Jun, Kamiyama, Naoya, Yokoo, Hideki, ...
Hamaguchi, Jun, Nakagawa, Hiroaki, Takahashi, Masato, Kudo, Takeaki, Kamiyama, Naoya, Sun, Bailong, ...
Nakahara, Taku, Hashimoto, Ryo, Nakagawa, Hiroaki, Monde, Kenji, Miura, Nobuaki, Nishimura, Shin-Ichiro
Glycobiology has been brought to public attention as a frontier in the post-genomic era. Structural information about glycans has been accumulating in the Protein Data Bank (PDB) for years. It has...
Kitamura, Hidemitsu, Iwakabe, Kenji, Yahata, Takashi, Nishimura, Shin-ichiro, Ohta, Akio, Ohmi, Yasushi, ...
The natural killer T (NKT) cell ligand α-galactosylceramide (α-GalCer) exhibits profound antitumor activities in vivo that resemble interleukin (IL)-12–mediated antitumor activities. Because of...
Averbeck, Nicole, Gao, Xiao-Dong, Nishimura, Shin-Ichiro, Dean, Neta
The second step of dolichol-linked oligosaccharide synthesis in the N-linked glycosylation pathway at the endoplasmic reticulum (ER) membrane is catalyzed by an unusual hetero-oligomeric...
Gao, Xiao-Dong, Moriyama, Satoru, Miura, Nobuaki, Dean, Neta, Nishimura, Shin-Ichiro
The second step of eukaryotic N-linked glycosylation in endoplasmic reticulum is catalyzed by an UDP-N-acetylglucosamine transferase that is comprised of two subunits, Alg13 and Alg14. The...