Shinji Hirotsune

NDEL1 Phosphorylation by Aurora-A Kinase Is Essential for Centrosomal Maturation, Separation, and TACC3 Recruitment (2007)

Mori, Daisuke, Yano, Yoshihisa, Toyo-oka, Kazuhito, Yoshida, Noriyuki, Yamada, Masami, Muramatsu, Masami, ...

NDEL1 is a binding partner of LIS1 that participates in the regulation of cytoplasmic dynein function and microtubule organization during mitotic cell division and neuronal migration. NDEL1...

Recruitment of Katanin P60 by Phosphorylated NDEL1, a LIS1 interacting protein, is Essential for Mitotic Cell Division and Neuronal Migration (2005)

Toyo-oka, Kazuhito, Sasaki, Shinji, Yano, Yoshihisa, Mori, Daisuke, Kobayashi, Takuya, Toyoshima, Yoko Y., ...

LIS1 is mutated in the human neuronal migration defect lissencephaly, and along with NDEL1 (formerly NUDEL), participates in the regulation of cytoplasmic dynein function during neuronal development....

Loss of the Max-interacting protein Mnt in mice results in decreased viability, defective embryonic growth and craniofacial defects: relevance to Miller-Dieker syndrome (2004)

Toyo-oka, Kazuhito, Hirotsune, Shinji, Gambello, Michael J., Zhou, Zi-Qiang, Olson, Lorin, Rosenfeld, Michael G., ...

The Mnt gene encodes a Mad-family bHLH transcription factor located on human 17p13.3. Mnt is one of twenty genes deleted in a heterozygous fashion in Miller-Dieker syndrome (MDS), a contiguous gene...

Loss of the Max-interacting protein Mnt in mice results in decreased viability, defective embryonic growth and craniofacial defects: relevance to Miller-Dieker syndrome (2004)

Toyo-oka, Kazuhito, Hirotsune, Shinji, Gambello, Michael J., Zhou, Zi-Qiang, Olson, Lorin, Rosenfeld, Michael G., ...

The Mnt gene encodes a Mad-family bHLH transcription factor located on human 17p13.3. Mnt is one of 20 genes deleted in a heterozygous fashion in Miller–Dieker syndrome (MDS), a contiguous gene...

Loss of the Max-interacting protein Mnt in mice results in decreased viability, defective embryonic growth and craniofacial defects: relevance to Miller-Dieker syndrome (2004)

Toyo-oka, Kazuhito, Hirotsune, Shinji, Gambello, Michael J., Zhou, Zi-Qiang, Olson, Lorin, Rosenfeld, Michael G., ...

The Mnt gene encodes a Mad-family bHLH transcription factor located on human 17p13.3. Mnt is one of twenty genes deleted in a heterozygous fashion in Miller-Dieker syndrome (MDS), a contiguous gene...

Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36 000 bovine ESTs (2001)

Takasuga, Akiko, Hirotsune, Shinji, Itoh, Reiko, Jitohzono, Ayako, Suzuki, Harumi, Aso, Hisashi, ...

We determined 36 310 bovine expressed sequence tag (EST) sequences using 10 different cDNA libraries. For massive EST sequencing, we devised a new system with two major features. First, we...

Null Mutation of PCLN-1/Claudin-16 Results in Bovine Chronic Interstitial Nephritis (2000)

Hirano, Takashi, Kobayashi, Naohiko, Itoh, Tomohito, Takasuga, Akiko, Nakamaru, Teruhiko, Hirotsune, Shinji, ...

Genomic Organization of the Murine Miller-Dieker/Lissencephaly Region: Conservation of Linkage with the Human Region (1997)

Hirotsune, Shinji, Pack, Svetlana D., Chong, Samuel S., Robbins, Christiane M., Pavan, William J., Ledbetter, David H., ...

Direct Determination of NotI Cleavage Sites in the Genomic DNA of Adult Mouse Kidney and Human Trophoblast Using Whole-Range Restriction Landmark Genomic Scanning (1994)

Imoto, Hirosumi, Hirotsune, Shinji, Muramatsu, Masami, Okuda, Kiyoji, Sugimoto, Osamu, Chapman, Verne M., ...

Restriction landmark genomic scanning (RLGS) is a method for visualizing restriction landmarks, employing direct labeling of restriction sites of genomic DNA and high-resolution two-dimensional...

Methylation profiles of genomic DNA of mouse developmental brain detected by restriction landmark genomic scanning (RLGS) method (1993)

Kawai, Jun, Hirotsune, Shinji, Hirose, Kenji, Fushiki, Shinji, Watanabe, Sachihiko, Hayashizaki, Yoshihide

Restriction landmark genomic scanning using methylation-sensitive endonucleases (RLGS-M) is a newly developed powerful method for systematic detection of DNA methylation. Using this method, we...

Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36 000 bovine ESTs

Takasuga, Akiko, Hirotsune, Shinji, Itoh, Reiko, Jitohzono, Ayako, Suzuki, Harumi, Aso, Hisashi, ...

We determined 36 310 bovine expressed sequence tag (EST) sequences using 10 different cDNA libraries. For massive EST sequencing, we devised a new system with two major features. First, we...

Deletion of Mnt leads to disrupted cell cycle control and tumorigenesis

Hurlin, Peter J., Zhou, Zi-Qiang, Toyo-oka, Kazuhito, Ota, Sara, Walker, William L., Hirotsune, Shinji, ...

Mnt is a Max-interacting transcriptional repressor that has been hypothesized to function as a Myc antagonist. To investigate Mnt function we deleted the Mnt gene in mice. Since mice lacking Mnt were...

Genomic Organization of the Murine Miller–Dieker/Lissencephaly Region: Conservation of Linkage with the Human Region

Hirotsune, Shinji, Pack, Svetlana D., Chong, Samuel S., Robbins, Christiane M., Pavan, William J., Ledbetter, David H., ...

Several human syndromes are associated with haploinsufficiency of chromosomal regions secondary to microdeletions. Isolated lissencephaly sequence (ILS), a human developmental disease characterized...

Null Mutation of PCLN-1/Claudin-16 Results in Bovine Chronic Interstitial Nephritis

Hirano, Takashi, Kobayashi, Naohiko, Itoh, Tomohito, Takasuga, Akiko, Nakamaru, Teruhiko, Hirotsune, Shinji, ...

Inherited chronic renal diseases are associated with failures in glomerular filtration and tubular resorption. Such failures invariably result from defects in selective filtration and absorption in...

Impaired Learning and Motor Behavior in Heterozygous Pafah1b1 (Lis1) Mutant Mice

Paylor, Richard, Hirotsune, Shinji, Gambello, Michael J., Yuva-Paylor, Lisa, Crawley, Jacqueline N., Wynshaw-Boris, Anthony

Heterozygous mutation or deletion of Pafah1b1 (LIS1) in humans is associated with syndromes with type 1 lissencephaly, a severe brain developmental disorder resulting from abnormal neuronal...

Complete Loss of Ndel1 Results in Neuronal Migration Defects and Early Embryonic Lethality

Sasaki, Shinji, Mori, Daisuke, Toyo-oka, Kazuhito, Chen, Amy, Garrett-Beal, Lisa, Muramatsu, Masami, ...

Regulation of cytoplasmic dynein and microtubule dynamics is crucial for both mitotic cell division and neuronal migration. NDEL1 was identified as a protein interacting with LIS1, the protein...

Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36 000 bovine ESTs

Takasuga, Akiko, Hirotsune, Shinji, Itoh, Reiko, Jitohzono, Ayako, Suzuki, Harumi, Aso, Hisashi, ...

We determined 36 310 bovine expressed sequence tag (EST) sequences using 10 different cDNA libraries. For massive EST sequencing, we devised a new system with two major features. First, we...

Deletion of Mnt leads to disrupted cell cycle control and tumorigenesis

Hurlin, Peter J., Zhou, Zi-Qiang, Toyo-oka, Kazuhito, Ota, Sara, Walker, William L., Hirotsune, Shinji, ...

Mnt is a Max-interacting transcriptional repressor that has been hypothesized to function as a Myc antagonist. To investigate Mnt function we deleted the Mnt gene in mice. Since mice lacking Mnt were...

Genomic Organization of the Murine Miller–Dieker/Lissencephaly Region: Conservation of Linkage with the Human Region

Hirotsune, Shinji, Pack, Svetlana D., Chong, Samuel S., Robbins, Christiane M., Pavan, William J., Ledbetter, David H., ...

Several human syndromes are associated with haploinsufficiency of chromosomal regions secondary to microdeletions. Isolated lissencephaly sequence (ILS), a human developmental disease characterized...

Null Mutation of PCLN-1/Claudin-16 Results in Bovine Chronic Interstitial Nephritis

Hirano, Takashi, Kobayashi, Naohiko, Itoh, Tomohito, Takasuga, Akiko, Nakamaru, Teruhiko, Hirotsune, Shinji, ...

Inherited chronic renal diseases are associated with failures in glomerular filtration and tubular resorption. Such failures invariably result from defects in selective filtration and absorption in...

Impaired Learning and Motor Behavior in Heterozygous Pafah1b1 (Lis1) Mutant Mice

Paylor, Richard, Hirotsune, Shinji, Gambello, Michael J., Yuva-Paylor, Lisa, Crawley, Jacqueline N., Wynshaw-Boris, Anthony

Heterozygous mutation or deletion of Pafah1b1 (LIS1) in humans is associated with syndromes with type 1 lissencephaly, a severe brain developmental disorder resulting from abnormal neuronal...

Refinement of a 400-kb Critical Region Allows Genotypic Differentiation between Isolated Lissencephaly, Miller-Dieker Syndrome, and Other Phenotypes Secondary to Deletions of 17p13.3

Cardoso, Carlos, Leventer, Richard J., Ward, Heather L., Toyo-oka, Kazuhito, Chung, June, Gross, Alyssa, ...

Deletions of 17p13.3, including the LIS1 gene, result in the brain malformation lissencephaly, which is characterized by reduced gyration and cortical thickening; however, the phenotype can vary from...

Complete Loss of Ndel1 Results in Neuronal Migration Defects and Early Embryonic Lethality

Sasaki, Shinji, Mori, Daisuke, Toyo-oka, Kazuhito, Chen, Amy, Garrett-Beal, Lisa, Muramatsu, Masami, ...

Regulation of cytoplasmic dynein and microtubule dynamics is crucial for both mitotic cell division and neuronal migration. NDEL1 was identified as a protein interacting with LIS1, the protein...

NDEL1 Phosphorylation by Aurora-A Kinase Is Essential for Centrosomal Maturation, Separation, and TACC3 Recruitment▿ †

Mori, Daisuke, Yano, Yoshihisa, Toyo-oka, Kazuhito, Yoshida, Noriyuki, Yamada, Masami, Muramatsu, Masami, ...

NDEL1 is a binding partner of LIS1 that participates in the regulation of cytoplasmic dynein function and microtubule organization during mitotic cell division and neuronal migration. NDEL1...

Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation

Toyo-oka, Kazuhito, Mori, Daisuke, Yano, Yoshihisa, Shiota, Masayuki, Iwao, Hiroshi, Goto, Hidemasa, ...

Protein phosphatase 4 catalytic subunit (PP4c) is a PP2A-related protein serine/threonine phosphatase with important functions in a variety of cellular processes, including microtubule (MT)...

LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein

Yamada, Masami, Toba, Shiori, Yoshida, Yuko, Haratani, Koji, Mori, Daisuke, Yano, Yoshihisa, ...

LIS1 was first identified as a gene mutated in human classical lissencephaly sequence. LIS1 is required for dynein activity, but the underlying mechanism is poorly understood. Here, we demonstrate...