Shinji Yamasaki

Molecular Typing of Vibrio cholerae   O1 Isolates from Thailand by Pulsed-field Gel Electrophoresis (2008)

Tapchaisri, Pramuan, Na-Ubol, Mathukorn, Tiyasuttipan, Watcharee, Chaiyaroj, Sansanee C., Yamasaki, Shinji, Wongsaroj, Thitima, ...

The aim of the present study was to genotypically characterize Vibrio cholerae   strains isolated from cholera patients in various provinces of Thailand. Two hundred and forty V. cholerae O1...

Cytokine and Nitric Oxide Production by Trypanosoma brucei Infection in Rats Fed Polyamine-Deficient Chow (2008)

Kazuhiko Nishimura, Michiko Yagi, Yoshihiro Ohnishi, Shinji Yamasaki

Feeding polyamine-deficient chow (PDC) to rats decreases blood polyamines, increases the activity of ornithine decarboxylase as an index of polyamine production, and increases resistance to...

Inhibition of Interleukin-12 Production by Trypanosoma brucei in Rat Macrophages (2008)

Kazuhiko Nishimura, Shinsuke Sakakibara, Kousuke Mitani, Jyoji Yamate, Yoshihiro Ohnishi, Shinji Yamasaki

The immune response of a host infected with Trypanosoma brucei is modulated by trypomastigotes. We examined the changes in cytokine production in T. brucei gambiense (Wellcome strain; WS) infected...

Effects of Heparin Administration on Trypanosoma brucei gambiense Infection in Rats (2005)

Kazuhiko Nishimura, Kensuke Shima, Masahiro Asakura, Yoshihiro Ohnishi, Shinji Yamasaki

We examined whether heparin administration influences in vivo trypanosome proliferation in infected rats. Administration of heparin every 8 hr via cardiac catheter inhibited growth of Trypanosoma...

DIFFERENTIAL EFFECTS OF INTERFERON-γ ON PRODUCTION OF TRYPANOSOME-DERIVED LYMPHOCYTE-TRIGGERING FACTOR BY TRYPANOSOMA BRUCEI GAMBIENSE AND TRYPANOSOMA BRUCEI BRUCEI (2004)

Kazuhiko Nishimura, Kanako Hamashita, Yasuyuki Okamoto, Fumiya Kawahara, Hideshi Ihara, Shunji Kozaki, ...

Trypanosome-derived lymphocyte-triggering factor (TLTF) produced by Trypanosoma brucei brucei stimulates production of interferon-γ (IFN-γ) by CD8+ T cells, and it is reported that, in turn, IFN-γ...

Clonal Diversity among Recently Emerged Strains of Vibrio parahaemolyticus O3:K6 Associated With Pandemic Spread

Bag, Prasanta K., Nandi, Suvobroto, Bhadra, Rupak K., Ramamurthy, T., Bhattacharya, S. K., Nishibuchi, M., ...

The genomes of the O3:K6 strains of Vibrio parahaemolyticus which abruptly emerged in Calcutta, India, in February 1996 and which demonstrated an unusual potential to spread and an enhanced...

Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India

Garg, Pallavi, Nandy, Ranjan K., Chaudhury, Papiya, Chowdhury, Nandini Roy, De, Keya, Ramamurthy, T., ...

The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular...

Virulence Genes in Environmental Strains of Vibrio cholerae

Chakraborty, Soumen, Mukhopadhyay, Asish K., Bhadra, Rupak Kumar, Ghosh, Amar Nath, Mitra, Rupak, Shimada, Toshio, ...

The virulence of a pathogen is dependent on a discrete set of genetic determinants and their well-regulated expression. The ctxAB and tcpA genes are known to play a cardinal role in maintaining...

Monoclonal Antibody to Shiga Toxin 2 Which Blocks Receptor Binding and Neutralizes Cytotoxicity

Nakao, Hiroshi, Kiyokawa, Nobutaka, Fujimoto, Junichiro, Yamasaki, Shinji, Takeda, Tae

A monoclonal antibody (MAb) was raised against Shiga toxin 2 (Stx2) of Escherichia coli O157:H7. MAb VTm1.1 belonged to the immunoglobulin G1 subclass and had a κ light chain, and it could...

Protective Effect of Lactobacillus casei Strain Shirota on Shiga Toxin-Producing Escherichia coli O157:H7 Infection in Infant Rabbits

Ogawa, Michinaga, Shimizu, Kensuke, Nomoto, Koji, Takahashi, Masatoshi, Watanuki, Masaaki, Tanaka, Ryuichiro, ...

We examined colonization patterns of Shiga toxin-producing Escherichia coli (STEC), concentrations of Shiga toxins (Stxs) and specific immunoglobulin A (lgA) against Stxs and STEC bacterial cell...

Identification and Characterization of a Newly Isolated Shiga Toxin 2-Converting Phage from Shiga Toxin-Producing Escherichia coli

Watarai, Masahisa, Sato, Toshio, Kobayashi, Midori, Shimizu, Takeshi, Yamasaki, Shinji, Tobe, Toru, ...

Shiga toxins 1 (Stx1) and 2 (Stx2) are encoded by toxin-converting bacteriophages of Stx-producing Escherichia coli (STEC), and so far two Stx1- and one Stx2-converting phages have been isolated from...

Isolation of Shigella dysenteriae Type 1 and S. flexneri Strains from Surface Waters in Bangladesh: Comparative Molecular Analysis of Environmental Shigella Isolates versus Clinical Strains

Faruque, Shah M., Khan, Rasel, Kamruzzaman, M., Yamasaki, Shinji, Ahmad, Q. Shafi, Azim, Tasnim, ...

Bacillary dysentery caused by Shigella species is a public health problem in developing countries including Bangladesh. Although, shigellae-contaminated food and drinks are often the source of the...

Cloning and Characterization of Genes Encoding Homologues of the B Subunit of Cholera Toxin and the Escherichia coli Heat-Labile Enterotoxin from Clinical Isolates of Citrobacter freundii and E. coli

Karasawa, Tadahiro, Ito, Hideaki, Tsukamoto, Teizo, Yamasaki, Shinji, Kurazono, Hisao, Faruque, Shah M., ...

We identified and characterized a gene encoding a homologue of the B subunits of cholera toxin (CTB) and heat-labile enterotoxin (LTB) of Escherichia coli from a clinical isolate of Citrobacter...

Genome Analysis of a Novel Shiga Toxin 1 (Stx1)-Converting Phage Which Is Closely Related to Stx2-Converting Phages but Not to Other Stx1-Converting Phages

Sato, Toshio, Shimizu, Takeshi, Watarai, Masahisa, Kobayashi, Midori, Kano, Shigeyuki, Hamabata, Takashi, ...

Two Stx-converting phages, designated Stx1φ and Stx2φ-II, were isolated from an Escherichia coli O157:H7 strain, Morioka V526, and their entire nucleotide sequences were determined. The genomes of...

Association of Cytolethal Distending Toxin Locus cdtB with Enteropathogenic Escherichia coli Isolated from Patients with Acute Diarrhea in Calcutta, India

Pandey, Mritunjay, Khan, Asis, Das, Suresh C., Sarkar, Bhaswati, Kahali, Soumen, Chakraborty, Subhra, ...

Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These...

Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh

Faruque, Shah M., Chowdhury, Nityananda, Khan, Rasel, Hasan, M. Rubayet, Nahar, Jebun, Islam, M. Johirul, ...

Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface...

Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India

Kahali, Soumen, Sarkar, Bhaswati, Rajendran, K., Khanam, Jasmina, Yamasaki, Shinji, Nandy, Ranjan K., ...

Enteroaggregative Escherichia coli (EAEC) is an important diarrheal enteropathogen defined by aggregative adherence to cultured epithelial cells. We have detected EAEC from 121 (6.6%) of 1,826...

Development of a PCR-Restriction Fragment Length Polymorphism Assay for the Epidemiological Analysis of Shiga Toxin-Producing Escherichia coli

Shima, Kensuke, Terajima, Jun, Sato, Toshio, Nishimura, Kazuhiko, Tamura, Kazumichi, Watanabe, Haruo, ...

Six characteristic regions (I to VI) were identified in Shiga toxin 2 (Stx2) phages (T. Sato, T. Shimizu, M. Watarai, M. Kobayashi, S. Kano, T. Hamabata, Y. Takeda, and S. Yamasaki, Gene 309:35-48,...

Genomic Sequence and Receptor for the Vibrio cholerae Phage KSF-1Φ: Evolutionary Divergence among Filamentous Vibriophages Mediating Lateral Gene Transfer

Faruque, Shah M., Bin Naser, Iftekhar, Fujihara, Kazutaka, Diraphat, Pornphan, Chowdhury, Nityananda, Kamruzzaman, M., ...

KSF-1Φ, a novel filamentous phage of Vibrio cholerae, supports morphogenesis of the RS1 satellite phage by heterologous DNA packaging and facilitates horizontal gene transfer. We analyzed the...

Clonal Diversity among Recently Emerged Strains of Vibrio parahaemolyticus O3:K6 Associated With Pandemic Spread

Bag, Prasanta K., Nandi, Suvobroto, Bhadra, Rupak K., Ramamurthy, T., Bhattacharya, S. K., Nishibuchi, M., ...

The genomes of the O3:K6 strains of Vibrio parahaemolyticus which abruptly emerged in Calcutta, India, in February 1996 and which demonstrated an unusual potential to spread and an enhanced...

Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India

Garg, Pallavi, Nandy, Ranjan K., Chaudhury, Papiya, Chowdhury, Nandini Roy, De, Keya, Ramamurthy, T., ...

The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular...

Virulence Genes in Environmental Strains of Vibrio cholerae

Chakraborty, Soumen, Mukhopadhyay, Asish K., Bhadra, Rupak Kumar, Ghosh, Amar Nath, Mitra, Rupak, Shimada, Toshio, ...

The virulence of a pathogen is dependent on a discrete set of genetic determinants and their well-regulated expression. The ctxAB and tcpA genes are known to play a cardinal role in maintaining...

Monoclonal Antibody to Shiga Toxin 2 Which Blocks Receptor Binding and Neutralizes Cytotoxicity

Nakao, Hiroshi, Kiyokawa, Nobutaka, Fujimoto, Junichiro, Yamasaki, Shinji, Takeda, Tae

A monoclonal antibody (MAb) was raised against Shiga toxin 2 (Stx2) of Escherichia coli O157:H7. MAb VTm1.1 belonged to the immunoglobulin G1 subclass and had a κ light chain, and it could...

Protective Effect of Lactobacillus casei Strain Shirota on Shiga Toxin-Producing Escherichia coli O157:H7 Infection in Infant Rabbits

Ogawa, Michinaga, Shimizu, Kensuke, Nomoto, Koji, Takahashi, Masatoshi, Watanuki, Masaaki, Tanaka, Ryuichiro, ...

We examined colonization patterns of Shiga toxin-producing Escherichia coli (STEC), concentrations of Shiga toxins (Stxs) and specific immunoglobulin A (lgA) against Stxs and STEC bacterial cell...

Identification and Characterization of a Newly Isolated Shiga Toxin 2-Converting Phage from Shiga Toxin-Producing Escherichia coli

Watarai, Masahisa, Sato, Toshio, Kobayashi, Midori, Shimizu, Takeshi, Yamasaki, Shinji, Tobe, Toru, ...

Shiga toxins 1 (Stx1) and 2 (Stx2) are encoded by toxin-converting bacteriophages of Stx-producing Escherichia coli (STEC), and so far two Stx1- and one Stx2-converting phages have been isolated from...

Isolation of Shigella dysenteriae Type 1 and S. flexneri Strains from Surface Waters in Bangladesh: Comparative Molecular Analysis of Environmental Shigella Isolates versus Clinical Strains

Faruque, Shah M., Khan, Rasel, Kamruzzaman, M., Yamasaki, Shinji, Ahmad, Q. Shafi, Azim, Tasnim, ...

Bacillary dysentery caused by Shigella species is a public health problem in developing countries including Bangladesh. Although, shigellae-contaminated food and drinks are often the source of the...

Cloning and Characterization of Genes Encoding Homologues of the B Subunit of Cholera Toxin and the Escherichia coli Heat-Labile Enterotoxin from Clinical Isolates of Citrobacter freundii and E. coli

Karasawa, Tadahiro, Ito, Hideaki, Tsukamoto, Teizo, Yamasaki, Shinji, Kurazono, Hisao, Faruque, Shah M., ...

We identified and characterized a gene encoding a homologue of the B subunits of cholera toxin (CTB) and heat-labile enterotoxin (LTB) of Escherichia coli from a clinical isolate of Citrobacter...

Genome Analysis of a Novel Shiga Toxin 1 (Stx1)-Converting Phage Which Is Closely Related to Stx2-Converting Phages but Not to Other Stx1-Converting Phages

Sato, Toshio, Shimizu, Takeshi, Watarai, Masahisa, Kobayashi, Midori, Kano, Shigeyuki, Hamabata, Takashi, ...

Two Stx-converting phages, designated Stx1φ and Stx2φ-II, were isolated from an Escherichia coli O157:H7 strain, Morioka V526, and their entire nucleotide sequences were determined. The genomes of...

Association of Cytolethal Distending Toxin Locus cdtB with Enteropathogenic Escherichia coli Isolated from Patients with Acute Diarrhea in Calcutta, India

Pandey, Mritunjay, Khan, Asis, Das, Suresh C., Sarkar, Bhaswati, Kahali, Soumen, Chakraborty, Subhra, ...

Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These...

Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh

Faruque, Shah M., Chowdhury, Nityananda, Khan, Rasel, Hasan, M. Rubayet, Nahar, Jebun, Islam, M. Johirul, ...

Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface...

Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India

Kahali, Soumen, Sarkar, Bhaswati, Rajendran, K., Khanam, Jasmina, Yamasaki, Shinji, Nandy, Ranjan K., ...

Enteroaggregative Escherichia coli (EAEC) is an important diarrheal enteropathogen defined by aggregative adherence to cultured epithelial cells. We have detected EAEC from 121 (6.6%) of 1,826...

Development of a PCR-Restriction Fragment Length Polymorphism Assay for the Epidemiological Analysis of Shiga Toxin-Producing Escherichia coli

Shima, Kensuke, Terajima, Jun, Sato, Toshio, Nishimura, Kazuhiko, Tamura, Kazumichi, Watanabe, Haruo, ...

Six characteristic regions (I to VI) were identified in Shiga toxin 2 (Stx2) phages (T. Sato, T. Shimizu, M. Watarai, M. Kobayashi, S. Kano, T. Hamabata, Y. Takeda, and S. Yamasaki, Gene 309:35-48,...

Genomic Sequence and Receptor for the Vibrio cholerae Phage KSF-1Φ: Evolutionary Divergence among Filamentous Vibriophages Mediating Lateral Gene Transfer

Faruque, Shah M., Bin Naser, Iftekhar, Fujihara, Kazutaka, Diraphat, Pornphan, Chowdhury, Nityananda, Kamruzzaman, M., ...

KSF-1Φ, a novel filamentous phage of Vibrio cholerae, supports morphogenesis of the RS1 satellite phage by heterologous DNA packaging and facilitates horizontal gene transfer. We analyzed the...

An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli

Asakura, Masahiro, Hinenoya, Atsushi, Alam, Mohammad S., Shima, Kensuke, Zahid, Shamim Hasan, Shi, Lei, ...

Cytolethal distending toxins (CDTs) are inhibitory cyclomodulins, which block eukaryotic cell proliferation and are produced by a diverse group of Gram-negative bacteria, including Escherichia coli...