Yonei, Taiji, Watarai, Shinobu, Okada, Yoshio, Yasuda, Tatsuji, Tsuji, Takao
Monoclonal antibodies were raised against urine proteins from diabetic patients. An antibody, YO-2, stained three protein bands with apparent molecular weights of 66, 49, and 36 kDa. These bands were...
Zhao, Dan-Dan, Watarai, Shinobu, Lee, Jin-tae, Kouchi, Shuuichi, Ohmori, Hitishi, Yasuda, Tatsuji
We compared the transfection efficiency of four types of positively charged liposomes composed of (i) N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine...
Availability of Liposomes as Drug Carriers to the Brain (2007)
Kobayashi, Kazuko, Han, Mei, Watarai, Shinobu, Yasuda, Tatsuji
Phospholipid vesicles, also known as liposomes, were examined for their ability to act as a drug carrier to the brain. 9-Amino-1,2,3,4-tetrahydroacridine (THA), a centrally acting...
Sensitive detection of ganglioside GD3 on the cell surface using liposome immune lysis assay. (2007)
Kobayashi, Kazuko, Watarai, Shinobu, Yasuda, Tatsuji
We developed a sensitive method for detection of glycosphingolipid (GSL) antigen(s) on the cell surface. As a model of GSL antigen, ganglioside GD3 was used. An IgM monoclonal antibody (DSG-1)...
Akiko Uemura, Shinobu Watarai, Yoshihiro Ohnishi, Hiroshi Kodama
Liposome-associated ganglioside antigens (ganglioside GM1 or bovine brain gangliosides) were prepared to facilitate the potential protective efficacy for Trypanosoma brucei. Mice were immunized with...
ISOLATION AND CHARACTERIZATION OF GANGLIOSIDES FROM TRYPANOSOMA BRUCEI (2004)
Akiko Uemura, Shinobu Watarai, Yasunori Kushi, Takeshi Kasama, Yoshihiro Ohnishi, Hiroshi Kodama
Gangliosides were isolated from Trypanosoma brucei and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4,...
Kaoru Nakata, Shinobu Watarai, Hiroshi Kodama, Tohru Gotanda, Akira Ito, Katsumi Kume
We have recently developed recombinant subunit vaccine consisting of second-generation schizont (2GS) membrane protein (rR7) of Leucocytozoon caulleryi. Chickens immunized with rR7 antigen acquired...
Watarai, Shinobu, Kushi, Yasunori, Shigeto, Rie, Misawa, Naoko, Eishi, Yoshinobu, Handa, Shizuo, ...
We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted...
Watarai, Shinobu, Kiura, Katsuyuki, Shigeto, Rie, Shibayama, Takuo, Kimura, Ikuro, Yasuda, Tatsuji
Three kinds of anti-GMl monoclonal antibodies, AGM-1, -2, and -3, of the IgM class were produced by the immunization of BALB/c mice with ganglioside GMl inserted into liposomes with Salmonella...
Production of Monoclonal Antibodies Specific for Ganglioside GD3 (1991)
Watarai, Shinobu, Onuma, Misao, Yasuda, Tatsuji
Four kinds of anti-GD3 monoclonal antibodies, DSG-1, -2, -3, and -4, of the IgM class were obtained by the immunization of BALB/c mice with enzootic bovine leukosis tumor tissue-derived ganglioside...
Suzuki, Yasuo, Nishi, Hiroshi, Hidari, Kazuya, Hirabayashi, Yoshio, Matsumoto, Makoto, Kobayashi, Toshiyuki, ...
A new monoclonal antibody (NS24) directed to the N-acetylneuraminylα2-3Galβ1-4GlcNAc residue in type II sugar chain of N-acetylneuraminyllactoneotetraosylceramide [sialylparagloboside,...
Watarai, Shinobu, Onuma, Misao, Yamamoto, Satoshi, Yasuda, Tatsuji
The effect of galactocerebroside 3′-sulfate (sulfatide) or cholesterol sulfate on syncytium formation induced by bovine immunodeficiency virus (BIV)-infected cells was investigated in vitro....
Glycosphingolipids of Normal Bovine and Enzootic Bovine Leukosis Lymph Node Cells (1986)
WATARAI, Shinobu, YASUDA, Tatsuji, ONUMA, Misao, HANDA, Shizuo
We analyzed glycosphingolipids from normal lymph node cells of seven cattle and lymph node cells of eight cattle with enzootic bovine leukosis. The neutral glycosphingolipids and gangliosides were...
Watarai, Shinobu, Yokota, Kenji, Kishimoto, Takumi, Kanadani, Tomohosa, Taketa, Kazuhisa, ...
The relationship between differential susceptibility to hemolytic-uremic syndrome (HUS) and levels of globotriaosylceramide (Gb3) in serum was studied in patients infected with verotoxin-producing...
Tajima, Shigeru, Takahashi, Masahiko, Takeshima, Shin-nosuke, Konnai, Satoru, Yin, Shan Ai, Watarai, Shinobu, ...
In a previous study, we identified an interesting mutant form of the Tax protein of bovine leukemia virus (BLV), designated D247G. This mutant protein strongly transactivated the long terminal repeat...
Mise, Koji, Akifusa, Sumio, Watarai, Shinobu, Ansai, Toshihiro, Nishihara, Tatsuji, Takehara, Tadamichi
Actinobacillus actinomycetemcomitans produces a toxin called cytolethal distending toxin (CDT), which causes host cell DNA damage leading to the induction of DNA damage checkpoint pathways. CDT...
Watarai, Shinobu, Yokota, Kenji, Kishimoto, Takumi, Kanadani, Tomohosa, Taketa, Kazuhisa, ...
The relationship between differential susceptibility to hemolytic-uremic syndrome (HUS) and levels of globotriaosylceramide (Gb3) in serum was studied in patients infected with verotoxin-producing...
Tajima, Shigeru, Takahashi, Masahiko, Takeshima, Shin-nosuke, Konnai, Satoru, Yin, Shan Ai, Watarai, Shinobu, ...
In a previous study, we identified an interesting mutant form of the Tax protein of bovine leukemia virus (BLV), designated D247G. This mutant protein strongly transactivated the long terminal repeat...
Mise, Koji, Akifusa, Sumio, Watarai, Shinobu, Ansai, Toshihiro, Nishihara, Tatsuji, Takehara, Tadamichi
Actinobacillus actinomycetemcomitans produces a toxin called cytolethal distending toxin (CDT), which causes host cell DNA damage leading to the induction of DNA damage checkpoint pathways. CDT...