Hiroaki Shirafuji, Xuenan Xuan, Isao Kimata, Yasuhiro Takashima, Shinya Fukumoto, Haruki Otsuka, ...
In this study, P23 of Cryptosporidium parvum sporozoites, an immunodominant surface protein, was stably expressed in Toxoplasma gondii (Tg/P23) and its protective effects were evaluated in a mouse...
Irungu Gaturaga, Bayin Chahan, Xuenan Xuan, Xiaohong Huang, Min Liao, Shinya Fukumoto, ...
The surface antigen 1–related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal...
Shinya Fukumoto, Yukiko Sekine, Xuenan Xuan, Ikuo Igarashi, Chihiro Sugimoto, Hideyuki Nagasawa, ...
The entire P50 gene encoding a surface protein of Babesia gibsoni was cloned into the bacteria expression vector pGEX-4T-3 and subsequently expressed in Escherichia coli as a glutathione...
Shinya Fukumoto, Xuenan Xuan, Ikuo Igarashi, Shoufa Zhang, Jennifer Mugisha, Takashi Ogata, ...
Canine red blood cell-substituted severe combined immune deficiency (Ca-RBC-SCID) mice were prepared for canine Babesia gibsoni infection. The Ca-RBC-SCID mice infected with B. gibsoni developed a...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....
Fukumoto, Shinya, Tamaki, Yoh, Shirafuji, Hiroaki, Harakawa, Shinji, Suzuki, Hiroshi, Xuan, Xuenan
This is a report of a vaccine trial directed against Babesia gibsoni infection in dogs with the use of the recombinant antigen P50. Dogs immunized with P50 showed partial protection manifested as a...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....
Fukumoto, Shinya, Tamaki, Yoh, Shirafuji, Hiroaki, Harakawa, Shinji, Suzuki, Hiroshi, Xuan, Xuenan
This is a report of a vaccine trial directed against Babesia gibsoni infection in dogs with the use of the recombinant antigen P50. Dogs immunized with P50 showed partial protection manifested as a...