Shoji Hatano

Freezing Tolerance of Transgenic Tobacco with Increased Content of Unsaturated Fatty Acid by Expressiong the CvFad3 Gene (2003)

Shimizu, Hideyuki, Furuya, Naoki, Suga, Koushirou, Honjoh, Ken-ichi, Miyamoto, Takahisa, Hatano, Shoji, ...

Two fatty acid desaturases were independently expressed in tobacco plants and freezing torelance of the transgenic plants was investigated: the two disaturases were CvFAD2, which desaturases...

Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27 (2003)

Honjoh, Ken-ichi, Suga, Koushirou, Shinohara, Fuminori, Maruyama, Isao, Miyamoto, Takahisa, Hatano, Shoji, ...

Protoplasts from Chlorella vulgaris K-73112 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanas Kl, Gluczyme, and Uskizyme. The formation of...

Three studies toward adaptive and evolutionary systems: Emergent Computation Model, Coevolutionary Model and Cooperating robots (1995)

Hisaaki Hatano, Yuji Sato, Yasusi Kanada, Shoji Hatano, Ryuichi Oka

M) [Kan 94b]. FAM is a method similar to simulated annealing but only uses local information. The performance is comparable to GSAT in large-scale graph coloring problems [Kan 95c]. Secondly,...

Molecular Cloning and Expression of Hardening-Induced Genes in Chlorella vulgaris C-27: the Most Abundant Clone Encodes a Late Embryogenesis Abundant Protein (1995)

Joh, Toshio, Honjoh, Ken-ichi, Yoshimoto, Makoto, Funabashi, Jun, Miyamoto, Takahisa, Hatano, Shoji

To investigate the effects of hardening on gene expression in Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27), a frost-hardy strain, 17 cDNA clones...

Isolation and Characterization of Hardening-Induced Proteins in Chlorella vulgaris C-27: Identification of Late Embryogenesis Abundant Proteins (1995)

Honjoh, Ken-ichi, Yoshimoto, Makoto, Joh, Toshio, Kajiwara, Taishin, Miyamoto, Takahisa, Hatano, Shoji

Hardening-induced soluble proteins of Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27) were isolated and purified by two-dimensional high-performance liquid...

Preparation of Protoplasts from Chiorella ellipsoidea C-27 (1992)

Hatano, Shoji, Joh, Toshio, Miyamoto, Takahisa, Yoshimoto, Makoto

Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell...

Effects of Low Temperature, Light and O2 on Chilling-sensitive and -resistant Strains in Chlorella ellipsoidea (1981)

Sadakane, Haruo, Ishibashi, Kazuko, Yoshimoto, Makoto, Hatano, Shoji

A low-temperature sensitive strain, Chlorella ellipsoidea Gerneck (IAM C-102), lost its chilling sensitivity during preservation. Cells of the original strain (low-temperature sensitive) and the...

Studies on frost hardiness in Chlorella ellipsoidea II. Effects of inhibitors of RNA and protein synthesis and surfactants on the process of hardening (1976)

Hatano, Shoji, Sadakane, Haruo, Tutumi, Masakazu, Watanabe, Tadao

Chlorella ellipsoidea cells at an intermediate stage in the ripening phase of the cell cycle were brought into contact with inhibitors of RNA and protein synthesis, and surfactants during or after...

Studies on frost hardiness in Chlorella ellipsoidea I. Development of frost hardiness of Chlorella ellipsoidea in synchronous culture (1976)

Hatano, Shoji, Sadakane, Haruo, Tutumi, Masakazu, Watanabe, Tadao

Cells of Chlorella ellipsoidea Gerneck (IAM C-27) were synchronously grown under a 28-hr light-14-hr dark regime at 25°C. The algal cells at different stages during the cell cycle were hardened at...

Transition of Lipid Synthesis from Chloroplasts to a Cytoplasmic System during Hardening in Chlorella ellipsoidea12

Hatano, Shoji, Kabata, Kiyotaka, Sadakane, Haruo

Chlorella ellipsoidea Gerneck (IAM C-27) was synchronously grown and cells at an intermediate stage in the ripening phase of the cell cycle were hardened at 3 C for 48 hours. At various times of...

Accumulation of Free Fatty Acids during Hardening of Chlorella ellipsoidea12

Hatano, Shoji, Kabata, Kiyotaka, Yoshimoto, Makoto, Sadakane, Haruo

Chlorella ellipsoidea Gerneck (IAM C-27) was synchronously grown, and cells at an intermediate stage in the ripening phase of the cell cycle were hardened at 3°C for 48 hours. A nonpolar lipid which...

Transition of Lipid Synthesis from Chloroplasts to a Cytoplasmic System during Hardening in Chlorella ellipsoidea12

Hatano, Shoji, Kabata, Kiyotaka, Sadakane, Haruo

Chlorella ellipsoidea Gerneck (IAM C-27) was synchronously grown and cells at an intermediate stage in the ripening phase of the cell cycle were hardened at 3 C for 48 hours. At various times of...

Accumulation of Free Fatty Acids during Hardening of Chlorella ellipsoidea12

Hatano, Shoji, Kabata, Kiyotaka, Yoshimoto, Makoto, Sadakane, Haruo

Chlorella ellipsoidea Gerneck (IAM C-27) was synchronously grown, and cells at an intermediate stage in the ripening phase of the cell cycle were hardened at 3°C for 48 hours. A nonpolar lipid which...