The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis
Bishop, Gerard J., Nomura, Takahito, Yokota, Takao, Harrison, Kate, Noguchi, Takahiro, Fujioka, Shozo, ...
Brassinosteroids (BRs) are steroidal plant hormones essential for normal plant growth and development. Mutants in the biosynthesis or perception of BRs are usually dwarf. The tomato Dwarf gene (D),...
BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis
Neff, Michael M., Nguyen, Serena M., Malancharuvil, Elizabeth J., Fujioka, Shozo, Noguchi, Takahiro, Seto, Hideharu, ...
The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in the photoreceptor phytochrome B (phyB). The adult phenotype of bas1-D phyB-4 double mutants mimics that...
Choe, Sunghwa, Dilkes, Brian P., Gregory, Brian D., Ross, Amanda S., Yuan, Heng, Noguchi, Takahiro, ...
Since the isolation and characterization of dwarf1-1 (dwf1-1) from a T-DNA insertion mutant population, phenotypically similar mutants, including deetiolated2 (det2), constitutive photomorphogenesis...
A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response1
Koka, Chala V., Cerny, R. Eric, Gardner, Randy G., Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...
The dumpy (dpy) mutant of tomato (Lycopersicon esculentum Mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. Application of brassinolide and castasterone rescued...
Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor1
Asami, Tadao, Min, Yong Ki, Nagata, Noriko, Yamagishi, Kazutoshi, Takatsuto, Suguru, Fujioka, Shozo, ...
Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by...
Biosynthetic Pathways of Brassinolide in Arabidopsis1
Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Tax, Frans E., Yoshida, Shigeo, ...
Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading...
Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Sakurai, Akira, Yoshida, Shigeo, Li, Jianming, ...
Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5α-reductase) acts early in the proposed...
Brassinosteroid-Insensitive Dwarf Mutants of Arabidopsis Accumulate Brassinosteroids1
Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Yoshida, Shigeo, Yuan, Heng, ...
Seven dwarf mutants resembling brassinosteroid (BR)-biosynthetic dwarfs were isolated that did not respond significantly to the application of exogenous BRs. Genetic and molecular analyses revealed...
Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements
Yamamoto, Ryo, Fujioka, Shozo, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, Fukuda, Hiroo
As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary...
Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...
Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...
Yamamuro, Chizuko, Ihara, Yoshihisa, Wu, Xiong, Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...
Brassinosteroids (BRs) are plant growth–promoting natural products required for plant growth and development. Physiological studies have demonstrated that exogenous BR, alone or in combination with...
The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning
Carland, Francine M., Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Nelson, Timothy
Vascular cell axialization refers to the uniform alignment of vascular strands. In the Arabidopsis cotyledon vascular pattern1 (cvp1) mutant, vascular cells are not arranged in parallel files and are...
An Early C-22 Oxidation Branch in the Brassinosteroid Biosynthetic Pathway
Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo
The natural occurrence of 22-hydroxylated steroids in cultured Catharanthus roseus cells and in Arabidopsis seedlings was investigated. Using full-scan gas chromatography-mass spectrometry analysis,...
Microarray Analysis of Brassinosteroid-Regulated Genes in Arabidopsis
Goda, Hideki, Shimada, Yukihisa, Asami, Tadao, Fujioka, Shozo, Yoshida, Shigeo
Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. Although insights into the functions of BRs have been provided by recent studies of biosynthesis and...
Choe, Sunghwa, Schmitz, Robert J., Fujioka, Shozo, Takatsuto, Suguru, Lee, Mi-Ok, Yoshida, Shigeo, ...
Mutants defective in the biosynthesis or signaling of brassinosteroids (BRs), plant steroid hormones, display dwarfism. Loss-of-function mutants for the gene encoding the plasma membrane-located BR...
Shimada, Yukihisa, Goda, Hideki, Nakamura, Ayako, Takatsuto, Suguru, Fujioka, Shozo, Yoshida, Shigeo
Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. There is only limited information on where BRs are synthesized and used. We studied the organ...
Sterols Regulate Development and Gene Expression in Arabidopsis1
He, Jun-Xian, Fujioka, Shozo, Li, Tsai-Chi, Kang, Shin Gene, Seto, Hideharu, Takatsuto, Suguru, ...
Sterols are important not only for structural components of eukaryotic cell membranes but also for biosynthetic precursors of steroid hormones. In plants, the diverse functions of sterol-derived...
The dominant non-gibberellin-responding dwarf mutant (D8) of maize accumulates native gibberellins
Fujioka, Shozo, Yamane, Hisakazu, Spray, Clive R., Katsumi, Masayuki, Phinney, Bernard O., Gaskin, Paul, ...
The endogenous gibberellins (GAs) were examined from young vegetative shoots of the dominant mutant, Dwarf-8, a GA-nonresponder, and normal maize; GA44, GA17, GA19, GA20, GA29, GA1, and GA8, members...
Hong, Zhi, Ueguchi-Tanaka, Miyako, Umemura, Kazuto, Uozu, Sakurako, Fujioka, Shozo, Takatsuto, Suguru, ...
We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of...
Turk, Edward M., Fujioka, Shozo, Seto, Hideharu, Shimada, Yukihisa, Takatsuto, Suguru, Yoshida, Shigeo, ...
Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase encoded by CYP72B1 has been implicated in...
Jang, Jyan-Chyun, Fujioka, Shozo, Tasaka, Masao, Seto, Hideharu, Takatsuto, Suguru, Ishii, Akira, ...
Here we report a novel Arabidopsis dwarf mutant, fackel-J79, whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons,...
Comprehensive Comparison of Auxin-Regulated and Brassinosteroid-Regulated Genes in Arabidopsis[w]
Goda, Hideki, Sawa, Shinichiro, Asami, Tadao, Fujioka, Shozo, Shimada, Yukihisa, Yoshida, Shigeo
Although numerous physiological studies have addressed the interactions between brassinosteroids and auxins, little is known about the underlying molecular mechanisms. Using an Affymetrix GeneChip...
Nomura, Takahito, Jager, Corinne E., Kitasaka, Yukiko, Takeuchi, Keiichi, Fukami, Motohiro, Yoneyama, Koichi, ...
The endogenous brassinosteroids in the dwarf mutant lk of pea (Pisum sativum) were quantified by gas chromatography-selected ion monitoring. The levels of castasterone, 6-deoxocastasterone, and...
Yamane, Hisakazu, Fujioka, Shozo, Spray, Clive R., Phinney, Bernard O., MacMillan, Jake, Gaskin, Paul, ...
Gibberellin A1 (GA1), 3-epi-GA1, GA4, GA9, 11α-hydroxyGA12, 12α-hydroxyGA12, GA15, GA17, GA19, GA20, GA25, GA37, GA40, GA58, GA69, GA70, and GA71 have been identified from Kovats retention indices...
Fujioka, Shozo, Yamane, Hisakazu, Spray, Clive R., Gaskin, Paul, Macmillan, Jake, Phinney, Bernard O., ...
Gibberellins A12 (GA12), GA53, GA44, GA19, GA17, GA20, GA29, GA1, and GA8 have been identified from extracts of vegetative shoots of normal (wild type) maize using full scan capillary gas...
Tanabe, Sumiyo, Ashikari, Motoyuki, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Yano, Masahiro, ...
We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, the D11 gene was isolated...
Gibberellin A3 Is Biosynthesized from Gibberellin A20 via Gibberellin A5 in Shoots of Zea mays L. 1
Fujioka, Shozo, Yamane, Hisakazu, Spray, Clive R., Phinney, Bernard O., Gaskin, Paul, MacMillan, Jake, ...
[17-13C,3H]-Labeled gibberellin A20 (GA20), GA5, and GA1 were fed to homozygous normal (+/+), heterozygous dominant dwarf (D8/+), and homozygous dominant dwarf (D8/D8) seedlings of Zea mays L....
Asami, Tadao, Mizutani, Masaharu, Shimada, Yukihisa, Goda, Hideki, Kitahata, Nobutaka, Sekimata, Katsuhiko, ...
Triadimefon (Bayleton), a widely used triazole-type fungicide, affects gibberellin (GA) biosynthesis and 14 alpha-demethylase in sterol biosynthesis. The present study revealed that the phenotype of...
The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids
Poppenberger, Brigitte, Fujioka, Shozo, Soeno, Kazuo, George, Gilu L., Vaistij, Fabián E., Hiranuma, Sayoko, ...
Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important...
Hong, Zhi, Ueguchi-Tanaka, Miyako, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Hasegawa, Yasuko, ...
We have identified a rice (Oryza sativa) brassinosteroid (BR)-deficient mutant, BR-deficient dwarf2 (brd2). The brd2 locus contains a single base deletion in the coding region of Dim/dwf1, a homolog...
TANMEI/EMB2757 Encodes a WD Repeat Protein Required for Embryo Development in Arabidopsis1
Yamagishi, Kazutoshi, Nagata, Noriko, Yee, Kelly Matsudaira, Braybrook, Siobhan A., Pelletier, Julie, Fujioka, Shozo, ...
We identified the Arabidopsis (Arabidopsis thaliana) tanmei/emb2757 (tan) mutation that causes defects in both embryo and seedling development. tan mutant embryos share many characteristics with the...
The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis
Bishop, Gerard J., Nomura, Takahito, Yokota, Takao, Harrison, Kate, Noguchi, Takahiro, Fujioka, Shozo, ...
Brassinosteroids (BRs) are steroidal plant hormones essential for normal plant growth and development. Mutants in the biosynthesis or perception of BRs are usually dwarf. The tomato Dwarf gene (D),...
BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis
Neff, Michael M., Nguyen, Serena M., Malancharuvil, Elizabeth J., Fujioka, Shozo, Noguchi, Takahiro, Seto, Hideharu, ...
The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in the photoreceptor phytochrome B (phyB). The adult phenotype of bas1-D phyB-4 double mutants mimics that...
Choe, Sunghwa, Dilkes, Brian P., Gregory, Brian D., Ross, Amanda S., Yuan, Heng, Noguchi, Takahiro, ...
Since the isolation and characterization of dwarf1-1 (dwf1-1) from a T-DNA insertion mutant population, phenotypically similar mutants, including deetiolated2 (det2), constitutive photomorphogenesis...
A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response1
Koka, Chala V., Cerny, R. Eric, Gardner, Randy G., Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...
The dumpy (dpy) mutant of tomato (Lycopersicon esculentum Mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. Application of brassinolide and castasterone rescued...
Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor1
Asami, Tadao, Min, Yong Ki, Nagata, Noriko, Yamagishi, Kazutoshi, Takatsuto, Suguru, Fujioka, Shozo, ...
Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by...
Biosynthetic Pathways of Brassinolide in Arabidopsis1
Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Tax, Frans E., Yoshida, Shigeo, ...
Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading...
Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Sakurai, Akira, Yoshida, Shigeo, Li, Jianming, ...
Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5α-reductase) acts early in the proposed...
Brassinosteroid-Insensitive Dwarf Mutants of Arabidopsis Accumulate Brassinosteroids1
Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Yoshida, Shigeo, Yuan, Heng, ...
Seven dwarf mutants resembling brassinosteroid (BR)-biosynthetic dwarfs were isolated that did not respond significantly to the application of exogenous BRs. Genetic and molecular analyses revealed...
Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements
Yamamoto, Ryo, Fujioka, Shozo, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, Fukuda, Hiroo
As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary...
Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...
Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...
Yamamuro, Chizuko, Ihara, Yoshihisa, Wu, Xiong, Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...
Brassinosteroids (BRs) are plant growth–promoting natural products required for plant growth and development. Physiological studies have demonstrated that exogenous BR, alone or in combination with...
The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning
Carland, Francine M., Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Nelson, Timothy
Vascular cell axialization refers to the uniform alignment of vascular strands. In the Arabidopsis cotyledon vascular pattern1 (cvp1) mutant, vascular cells are not arranged in parallel files and are...
An Early C-22 Oxidation Branch in the Brassinosteroid Biosynthetic Pathway
Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo
The natural occurrence of 22-hydroxylated steroids in cultured Catharanthus roseus cells and in Arabidopsis seedlings was investigated. Using full-scan gas chromatography-mass spectrometry analysis,...
Microarray Analysis of Brassinosteroid-Regulated Genes in Arabidopsis
Goda, Hideki, Shimada, Yukihisa, Asami, Tadao, Fujioka, Shozo, Yoshida, Shigeo
Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. Although insights into the functions of BRs have been provided by recent studies of biosynthesis and...
Choe, Sunghwa, Schmitz, Robert J., Fujioka, Shozo, Takatsuto, Suguru, Lee, Mi-Ok, Yoshida, Shigeo, ...
Mutants defective in the biosynthesis or signaling of brassinosteroids (BRs), plant steroid hormones, display dwarfism. Loss-of-function mutants for the gene encoding the plasma membrane-located BR...
Shimada, Yukihisa, Goda, Hideki, Nakamura, Ayako, Takatsuto, Suguru, Fujioka, Shozo, Yoshida, Shigeo
Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. There is only limited information on where BRs are synthesized and used. We studied the organ...
Sterols Regulate Development and Gene Expression in Arabidopsis1
He, Jun-Xian, Fujioka, Shozo, Li, Tsai-Chi, Kang, Shin Gene, Seto, Hideharu, Takatsuto, Suguru, ...
Sterols are important not only for structural components of eukaryotic cell membranes but also for biosynthetic precursors of steroid hormones. In plants, the diverse functions of sterol-derived...
The dominant non-gibberellin-responding dwarf mutant (D8) of maize accumulates native gibberellins
Fujioka, Shozo, Yamane, Hisakazu, Spray, Clive R., Katsumi, Masayuki, Phinney, Bernard O., Gaskin, Paul, ...
The endogenous gibberellins (GAs) were examined from young vegetative shoots of the dominant mutant, Dwarf-8, a GA-nonresponder, and normal maize; GA44, GA17, GA19, GA20, GA29, GA1, and GA8, members...
Hong, Zhi, Ueguchi-Tanaka, Miyako, Umemura, Kazuto, Uozu, Sakurako, Fujioka, Shozo, Takatsuto, Suguru, ...
We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of...
Turk, Edward M., Fujioka, Shozo, Seto, Hideharu, Shimada, Yukihisa, Takatsuto, Suguru, Yoshida, Shigeo, ...
Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase encoded by CYP72B1 has been implicated in...
Jang, Jyan-Chyun, Fujioka, Shozo, Tasaka, Masao, Seto, Hideharu, Takatsuto, Suguru, Ishii, Akira, ...
Here we report a novel Arabidopsis dwarf mutant, fackel-J79, whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons,...
Comprehensive Comparison of Auxin-Regulated and Brassinosteroid-Regulated Genes in Arabidopsis[w]
Goda, Hideki, Sawa, Shinichiro, Asami, Tadao, Fujioka, Shozo, Shimada, Yukihisa, Yoshida, Shigeo
Although numerous physiological studies have addressed the interactions between brassinosteroids and auxins, little is known about the underlying molecular mechanisms. Using an Affymetrix GeneChip...
Nomura, Takahito, Jager, Corinne E., Kitasaka, Yukiko, Takeuchi, Keiichi, Fukami, Motohiro, Yoneyama, Koichi, ...
The endogenous brassinosteroids in the dwarf mutant lk of pea (Pisum sativum) were quantified by gas chromatography-selected ion monitoring. The levels of castasterone, 6-deoxocastasterone, and...
Yamane, Hisakazu, Fujioka, Shozo, Spray, Clive R., Phinney, Bernard O., MacMillan, Jake, Gaskin, Paul, ...
Gibberellin A1 (GA1), 3-epi-GA1, GA4, GA9, 11α-hydroxyGA12, 12α-hydroxyGA12, GA15, GA17, GA19, GA20, GA25, GA37, GA40, GA58, GA69, GA70, and GA71 have been identified from Kovats retention indices...
Fujioka, Shozo, Yamane, Hisakazu, Spray, Clive R., Gaskin, Paul, Macmillan, Jake, Phinney, Bernard O., ...
Gibberellins A12 (GA12), GA53, GA44, GA19, GA17, GA20, GA29, GA1, and GA8 have been identified from extracts of vegetative shoots of normal (wild type) maize using full scan capillary gas...