Parallel gene synthesis in a microfluidic device (2007)
Kong, David S., Carr, Peter A., Chen, Lu, Zhang, Shuguang, Jacobson, Joseph M.
The ability to synthesize custom de novo DNA constructs rapidly, accurately and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous...
Building from the Bottom Up (2007)
Designed materials and new tools hold the key for future science and technologies. Building materials from the bottom up is complementary to traditional top-down materials processing, but requires a...
Nano hemostat solution: immediate hemostasis at the nanoscale (2006)
Ellis-Behnke, Rutledge G., Liang, Yu-Xiang, Tay, David K.C., Kau, Phillis W.F., Schneider, Gerald E., Zhang, Shuguang, ...
Hemostasis is a major problem in surgical procedures and after major trauma. There are few effective methods to stop bleeding without causing secondary damage. We used a self-assembling peptide that...
Design of Nanostructured Biological Materials Through Self-Assembly of Peptides and Proteins (2006)
Zhang, Shuguang, Marini, Davide M., Hwang, Wonmuk, Santoso, Steve
Introduction Self-assembly can be defined as the spontaneous organization of individual components into an ordered structure without human intervention [1""]. The key elements of molecular...
Patrick Kiley, Xiaojun Zhao, Michael Vaughn, Marc A. Baldo, Barry D. Bruce, Shuguang Zhang
A designer peptide detergent may facilitate the study of diverse types of membrane proteins.
Patrick Kiley, Xiaojun Zhao, Michael Vaughn, Marc A. Baldo, Barry D. Bruce, Shuguang Zhang
We used a class of designed peptide detergents to stabilize photosystem I (PS-I) upon extended drying under N2 on a gold-coated-Ni-NTA glass surface. PS-I is a chlorophyll-containing membrane protein...
Protein-mediated error correction for de novo DNA synthesis (2004)
Carr, Peter A., Park, Jason S., Lee, Yoon-Jae, Yu, Tiffany, Zhang, Shuguang, Jacobson, Joseph M.
The availability of inexpensive, on demand synthetic DNA has enabled numerous powerful applications in biotechnology, in turn driving considerable present interest in the de novo synthesis of...
A New Class of Self-Complementary Beta Sheet Oligopeptide- Based Biomaterials (1998)
Rich, Alexander, Zhang, Shuguang
Mechanisms and functions of a new class of biological materials discovered through self-assembly of ionic self-complementary oligopeptides were studied, and three types of peptides were classified....
Self-Assembling Ionic Oligopeptides (1998)
Rich, Alexander, Zhang, Shuguang
Understanding new materials at the molecular level has become increasingly critical for a new generation of nanomaterials for nanotechnology, namely, the design, synthesis and fabrication of...
Peptide Nanofilament Engineering (1998)
Rich, Alexander, Zhang, Shuguang
This laboratory studied four systems involving molecular self-assembly during this project period. Each system will open a new avenue of research in developing novel applications for use in...
Electron Transfer of Redox Enzymes to Inorganic Electrodes (1998)
This project was to develop a protein and peptide-based nanowire and nanoswitch with defined length and properties for a broad spectrum of application in nanobiotechnology. These nanowires were used...
Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds
Holmes, Todd C., De Lacalle, Sonsoles, Su, Xing, Liu, Guosong, Rich, Alexander, Zhang, Shuguang
A new type of self-assembling peptide (sapeptide) scaffolds that serve as substrates for neurite outgrowth and synapse formation is described. These peptide-based scaffolds are amenable to molecular...
Direct conversion of an oligopeptide from a β-sheet to an α-helix: A model for amyloid formation
Zhang, Shuguang, Rich, Alexander
A 16-amino acid oligopeptide forms a stable β-sheet structure in water. In physiological solutions it is able to self-assemble to form a macroscopic matrix that stains with Congo red. On raising the...
Molecular self-assembly of surfactant-like peptides to form nanotubes and nanovesicles
Vauthey, Sylvain, Santoso, Steve, Gong, Haiyan, Watson, Nicki, Zhang, Shuguang
Several surfactant-like peptides undergo self-assembly to form nanotubes and nanovesicles having an average diameter of 30–50 nm with a helical twist. The peptide monomer contains 7–8 residues...
Kinetic control of dimer structure formation in amyloid fibrillogenesis
Hwang, Wonmuk, Zhang, Shuguang, Kamm, Roger D., Karplus, Martin
Amyloid fibril formation involves nonfibrillar oligomeric intermediates, which are important as possible cytotoxic species in neurodegenerative diseases. However, their transient nature and...
Indole-Diterpene Gene Cluster from Aspergillus flavus
Zhang, Shuguang, Monahan, Brendon J., Tkacz, Jan S., Scott, Barry
Aflatrem is a potent tremorgenic mycotoxin produced by the soil fungus Aspergillus flavus and is a member of a large structurally diverse group of secondary metabolites known as indole-diterpenes. By...
Protein-mediated error correction for de novo DNA synthesis
Carr, Peter A., Park, Jason S., Lee, Yoon-Jae, Yu, Tiffany, Zhang, Shuguang, Jacobson, Joseph M.
The availability of inexpensive, on demand synthetic DNA has enabled numerous powerful applications in biotechnology, in turn driving considerable present interest in the de novo synthesis of...
Dynamic reassembly of peptide RADA16 nanofiber scaffold
Yokoi, Hidenori, Kinoshita, Takatoshi, Zhang, Shuguang
Nanofiber structures of some peptides and proteins as biological materials have been studied extensively, but their molecular mechanism of self-assembly and reassembly still remains unclear. We...
Kiley, Patrick, Zhao, Xiaojun, Vaughn, Michael, Baldo, Marc A, Bruce, Barry D, Zhang, Shuguang
We used a class of designed peptide detergents to stabilize photosystem I (PS-I) upon extended drying under N2 on a gold-coated-Ni-NTA glass surface. PS-I is a chlorophyll-containing membrane protein...
Ellis-Behnke, Rutledge G., Liang, Yu-Xiang, You, Si-Wei, Tay, David K. C., Zhang, Shuguang, So, Kwok-Fai, ...
Nanotechnology is often associated with materials fabrication, microelectronics, and microfluidics. Until now, the use of nanotechnology and molecular self assembly in biomedicine to repair injured...
Davis, Michael E., Hsieh, Patrick C. H., Takahashi, Tomosaburo, Song, Qing, Zhang, Shuguang, Kamm, Roger D., ...
Strategies for cardiac repair include injection of cells, but these approaches have been hampered by poor cell engraftment, survival, and differentiation. To address these shortcomings for the...
Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds
Holmes, Todd C., De Lacalle, Sonsoles, Su, Xing, Liu, Guosong, Rich, Alexander, Zhang, Shuguang
A new type of self-assembling peptide (sapeptide) scaffolds that serve as substrates for neurite outgrowth and synapse formation is described. These peptide-based scaffolds are amenable to molecular...
Direct conversion of an oligopeptide from a β-sheet to an α-helix: A model for amyloid formation
Zhang, Shuguang, Rich, Alexander
A 16-amino acid oligopeptide forms a stable β-sheet structure in water. In physiological solutions it is able to self-assemble to form a macroscopic matrix that stains with Congo red. On raising the...
Molecular self-assembly of surfactant-like peptides to form nanotubes and nanovesicles
Vauthey, Sylvain, Santoso, Steve, Gong, Haiyan, Watson, Nicki, Zhang, Shuguang
Several surfactant-like peptides undergo self-assembly to form nanotubes and nanovesicles having an average diameter of 30–50 nm with a helical twist. The peptide monomer contains 7–8 residues...
Kinetic control of dimer structure formation in amyloid fibrillogenesis
Hwang, Wonmuk, Zhang, Shuguang, Kamm, Roger D., Karplus, Martin
Amyloid fibril formation involves nonfibrillar oligomeric intermediates, which are important as possible cytotoxic species in neurodegenerative diseases. However, their transient nature and...
Indole-Diterpene Gene Cluster from Aspergillus flavus
Zhang, Shuguang, Monahan, Brendon J., Tkacz, Jan S., Scott, Barry
Aflatrem is a potent tremorgenic mycotoxin produced by the soil fungus Aspergillus flavus and is a member of a large structurally diverse group of secondary metabolites known as indole-diterpenes. By...
Protein-mediated error correction for de novo DNA synthesis
Carr, Peter A., Park, Jason S., Lee, Yoon-Jae, Yu, Tiffany, Zhang, Shuguang, Jacobson, Joseph M.
The availability of inexpensive, on demand synthetic DNA has enabled numerous powerful applications in biotechnology, in turn driving considerable present interest in the de novo synthesis of...
Dynamic reassembly of peptide RADA16 nanofiber scaffold
Yokoi, Hidenori, Kinoshita, Takatoshi, Zhang, Shuguang
Nanofiber structures of some peptides and proteins as biological materials have been studied extensively, but their molecular mechanism of self-assembly and reassembly still remains unclear. We...
Kiley, Patrick, Zhao, Xiaojun, Vaughn, Michael, Baldo, Marc A, Bruce, Barry D, Zhang, Shuguang
We used a class of designed peptide detergents to stabilize photosystem I (PS-I) upon extended drying under N2 on a gold-coated-Ni-NTA glass surface. PS-I is a chlorophyll-containing membrane protein...
Ellis-Behnke, Rutledge G., Liang, Yu-Xiang, You, Si-Wei, Tay, David K. C., Zhang, Shuguang, So, Kwok-Fai, ...
Nanotechnology is often associated with materials fabrication, microelectronics, and microfluidics. Until now, the use of nanotechnology and molecular self assembly in biomedicine to repair injured...
Davis, Michael E., Hsieh, Patrick C. H., Takahashi, Tomosaburo, Song, Qing, Zhang, Shuguang, Kamm, Roger D., ...
Strategies for cardiac repair include injection of cells, but these approaches have been hampered by poor cell engraftment, survival, and differentiation. To address these shortcomings for the...
Gelain, Fabrizio, Bottai, Daniele, Vescovi, Angleo, Zhang, Shuguang
Biomedical researchers have become increasingly aware of the limitations of conventional 2-dimensional tissue cell culture systems, including coated Petri dishes, multi-well plates and slides, to...
Horii, Akihiro, Wang, Xiumei, Gelain, Fabrizio, Zhang, Shuguang
A class of self-assembling peptide nanofiber scaffolds has been shown to be an excellent biological material for 3-dimension cell culture and stimulating cell migration into the scaffold, as well as...
Designer short peptide surfactants stabilize G protein-coupled receptor bovine rhodopsin
Zhao, Xiaojun, Nagai, Yusuke, Reeves, Philip J., Kiley, Patrick, Khorana, H. Gobind, Zhang, Shuguang
Membrane proteins play vital roles in every aspect of cellular activities. To study diverse membrane proteins, it is crucial to select the right surfactants to stabilize them for analysis. Despite...
Tuning Curvature and Stability of Monoolein Bilayers by Designer Lipid-Like Peptide Surfactants
Yaghmur, Anan, Laggner, Peter, Zhang, Shuguang, Rappolt, Michael
This study reports the effect of loading four different charged designer lipid-like short anionic and cationic peptide surfactants on the fully hydrated monoolein (MO)-based Pn3m phase (Q224). The...
Parallel gene synthesis in a microfluidic device
Kong, David S., Carr, Peter A., Chen, Lu, Zhang, Shuguang, Jacobson, Joseph M.
The ability to synthesize custom de novo DNA constructs rapidly, accurately and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous...
Ultra-small-sample molecular structure detection using microslot waveguide nuclear spin resonance
Maguire, Yael, Chuang, Isaac L., Zhang, Shuguang, Gershenfeld, Neil
We here report on the design of a planar microslot waveguide NMR probe with an induction element that can be fabricated at scales from centimeters to nanometers to allow analysis of biomolecules at...
Structural Dynamic of a Self-Assembling Peptide d-EAK16 Made of Only D-Amino Acids
Luo, Zhongli, Zhao, Xiaojun, Zhang, Shuguang
We here report systematic study of structural dynamics of a 16-residue self-assembling peptide d-EAK16 made of only D-amino acids. We compare these results with its chiral counterpart L-form,...
Takayama, Hidehito, Chelikani, Prashen, Reeves, Philip J., Zhang, Shuguang, Khorana, H. Gobind
The study of membrane protein structure and function requires their high-level expression and purification in fully functional form. We previously used a tetracycline-inducible stable mammalian cell...
Cook, Brian L., Ernberg, Karin E., Chung, Hyeyoun, Zhang, Shuguang
In order to begin to study the structural and functional mechanisms of olfactory receptors, methods for milligram-scale purification are required. Here we demonstrate the production and expression of...
Love, Christopher J., Zhang, Shuguang, Mershin, Andreas
It has long been known that there is a sustained electrical potential (voltage) difference between the xylem of many plants and their surrounding soil, but the mechanism behind this voltage has...
Ren, Hui, Yu, Daoyong, Ge, Baosheng, Cook, Brian, Xu, Zhinan, Zhang, Shuguang
Chemokine receptors belong to a class of integral membrane G-protein coupled receptors (GPCRs) and are responsible for transmitting signals from the extracellular environment. However, the structural...
Kaiser, Liselotte, Graveland-Bikker, Johanna, Steuerwald, Dirk, Vanberghem, Mélanie, Herlihy, Kara, Zhang, Shuguang
High-level production of membrane proteins, particularly of G protein-coupled receptors (GPCRs) in heterologous cell systems encounters a number of difficulties from their inherent hydrophobicity in...
Koutsopoulos, Sotirios, Unsworth, Larry D., Nagai, Yusuke, Zhang, Shuguang
The release kinetics for a variety of proteins of a wide range of molecular mass, hydrodynamic radii, and isoelectric points through a nanofiber hydrogel scaffold consisting of designer...