Siamon Gordon

The Mannose Receptor Mediates Dengue Virus Infection of Macrophages (2008)

Joanna L. Miller, Luisa Martinez-Pomares, Catherine M. Radcliffe, Raymond A. Dwek, Pauline M. Rudd, ...

Macrophages (MØ) and mononuclear phagocytes are major targets of infection by dengue virus (DV), a mosquito-borne flavivirus that can cause haemorrhagic fever in humans. To our knowledge, we show...

RNA Interference Mutant Induction In Vivo Demonstrates the Essential Nature of Trypanosome Flagellar Function during Mammalian Infection. (2007)

Griffiths, Samantha, Portman, Neil, Taylor, Philip R., Gordon, Siamon, Ginger, Michael L., Gull , Keith

We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell...

Expression of the class A macrophage scavenger receptor on specific subpopulations of murine dendritic cells limits their endotoxin response (2006)

Becker, Mike, Cotena, Alessia, Gordon, Siamon, Platt, Nick

Dendritic cells (DC) function at the interface of innate and acquired immunity and are uniquely sensitive to specific stimuli. Pattern recognition receptors (PRR) on these cells are critically...

Expression of the class A macrophage scavenger receptor on specific subpopulations of murine dendritic cells limits their endotoxin response (2006)

Becker, Mike, Cotena, Alessia, Gordon, Siamon, Platt, Nick

Dendritic cells (DC) function at the interface of innate and acquired immunity and are uniquely sensitive to specific stimuli. Pattern recognition receptors (PRR) on these cells are critically...

The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies of Aspergillus fumigatus (2005)

Chad Steele, Rekha R. Rapaka, Allison Metz, Shannon M. Pop, David L. Williams, Siamon Gordon, ...

Alveolar macrophages represent a first-line innate host defense mechanism for clearing inhaled Aspergillus fumigatus from the lungs, yet contradictory data exist as to which alveolar macrophage...

The class A macrophage scavenger receptor attenuates CXC chemokine production and the early infiltration of neutrophils in sterile peritonitis (2004)

Cotena, Alessia, Gordon, Siamon, Platt, Nick

The macrophage scavenger receptor (SR-A) is a multifunctional receptor that is associated with several important pathological conditions, including atherosclerosis. In this study, we show, using a...

Macrophage Class A Scavenger Receptor-Mediated Phagocytosis of Escherichia coli: Role of Cell Heterogeneity, Microbial Strain, and Culture Conditions In Vitro

Peiser, Leanne, Gough, Peter J., Kodama, Tatsuhiko, Gordon, Siamon

Macrophage class A scavenger receptors (SR-AI and SR-AII) contribute to host defense by binding polyanionic ligands such as lipopolysaccharide and lipoteichoic acid. SR-A knockout (SR-A−/−) mice...

Characteristics of Invasive Candidiasis in Gamma Interferon- and Interleukin-4-Deficient Mice: Role of Macrophages in Host Defense against Candida albicans

Káposzta, Rita, Tree, Peter, Maródi, László, Gordon, Siamon

Murine models of invasive candidiasis were used to study the in vivo importance of gamma interferon (IFN-γ) and interleukin-4 (IL-4) in host defense against Candida albicans and to characterize the...

The Class A Macrophage Scavenger Receptor Is a Major Pattern Recognition Receptor for Neisseria meningitidis Which Is Independent of Lipopolysaccharide and Not Required for Secretory Responses

Peiser, Leanne, Makepeace, Katherine, Hollinshead, Michael, Coull, Philip, Plested, Joyce, ...

Macrophages (Mφ) play a key role in the pathogenesis of invasive meningococcal infections. The roles of two pattern recognition molecules, the Mφ scavenger receptor (SR-A) and Toll-like receptor 4...

Inactivation of the F4/80 Glycoprotein in the Mouse Germ Line

Schaller, Evelyne, Macfarlane, Alison J., Rupec, Rudolf A., Gordon, Siamon, McKnight, Andrew J., Pfeffer, Klaus

Macrophages play a crucial role in the defense against pathogens. Distinct macrophage populations can be defined by the expression of restricted cell surface proteins. Resident tissue macrophages,...

Neutralization of Infectivity of Diverse R5 Clinical Isolates of Human Immunodeficiency Virus Type 1 by gp120-Binding 2′F-RNA Aptamers

Khati, Makobetsa, Schüman, Michael, Ibrahim, Jamal, Sattentau, Quentin, Gordon, Siamon, James, William

Human immunodeficiency virus type 1 (HIV-1) has evolved a number of strategies to resist current antiretroviral drugs and the selection pressures of humoral and cellular adaptive immunity. For...

Development of a specific system for targeting protein to metallophilic macrophages

Taylor, Philip R., Zamze, Susanne, Stillion, Richard J., Wong, Simon Y. C., Gordon, Siamon, Martinez-Pomares, Luisa

The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL+ cells). In naïve...

Degradation of basic protein in myelin by neutral proteases secreted by stimulated macrophages: A possible mechanism of inflammatory demyelination

Cammer, Wendy, Bloom, Barry R., Norton, William T., Gordon, Siamon

In inflammatory demyelinating diseases such as multiple sclerosis and experimental allergic encephalomyelitis, myelin destruction occurs in the vicinity of infiltrating mononuclear cells. The...

Cytokine Responses in Severe Acute Respiratory Syndrome Coronavirus-Infected Macrophages In Vitro: Possible Relevance to Pathogenesis

Cheung, Chung Y., Poon, Leo L. M., Ng, Iris H. Y., Luk, Winsie, Sia, Sin-Fun, Wu, Mavis H. S., ...

The pathogenesis of severe acute respiratory syndrome (SARS) remains unclear. Macrophages are key sentinel cells in the respiratory system, and it is therefore relevant to compare the responses of...

The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies of Aspergillus fumigatus

Steele, Chad, Rapaka, Rekha R, Metz, Allison, Pop, Shannon M, Williams, David L, Gordon, Siamon, ...

Alveolar macrophages represent a first-line innate host defense mechanism for clearing inhaled Aspergillus fumigatus from the lungs, yet contradictory data exist as to which alveolar macrophage...

Macrophage Class A Scavenger Receptor-Mediated Phagocytosis of Escherichia coli: Role of Cell Heterogeneity, Microbial Strain, and Culture Conditions In Vitro

Peiser, Leanne, Gough, Peter J., Kodama, Tatsuhiko, Gordon, Siamon

Macrophage class A scavenger receptors (SR-AI and SR-AII) contribute to host defense by binding polyanionic ligands such as lipopolysaccharide and lipoteichoic acid. SR-A knockout (SR-A−/−) mice...

Characteristics of Invasive Candidiasis in Gamma Interferon- and Interleukin-4-Deficient Mice: Role of Macrophages in Host Defense against Candida albicans

Káposzta, Rita, Tree, Peter, Maródi, László, Gordon, Siamon

Murine models of invasive candidiasis were used to study the in vivo importance of gamma interferon (IFN-γ) and interleukin-4 (IL-4) in host defense against Candida albicans and to characterize the...

The Class A Macrophage Scavenger Receptor Is a Major Pattern Recognition Receptor for Neisseria meningitidis Which Is Independent of Lipopolysaccharide and Not Required for Secretory Responses

Peiser, Leanne, Makepeace, Katherine, Hollinshead, Michael, Coull, Philip, Plested, Joyce, ...

Macrophages (Mφ) play a key role in the pathogenesis of invasive meningococcal infections. The roles of two pattern recognition molecules, the Mφ scavenger receptor (SR-A) and Toll-like receptor 4...

Inactivation of the F4/80 Glycoprotein in the Mouse Germ Line

Schaller, Evelyne, Macfarlane, Alison J., Rupec, Rudolf A., Gordon, Siamon, McKnight, Andrew J., Pfeffer, Klaus

Macrophages play a crucial role in the defense against pathogens. Distinct macrophage populations can be defined by the expression of restricted cell surface proteins. Resident tissue macrophages,...

Neutralization of Infectivity of Diverse R5 Clinical Isolates of Human Immunodeficiency Virus Type 1 by gp120-Binding 2′F-RNA Aptamers

Khati, Makobetsa, Schüman, Michael, Ibrahim, Jamal, Sattentau, Quentin, Gordon, Siamon, James, William

Human immunodeficiency virus type 1 (HIV-1) has evolved a number of strategies to resist current antiretroviral drugs and the selection pressures of humoral and cellular adaptive immunity. For...

Development of a specific system for targeting protein to metallophilic macrophages

Taylor, Philip R., Zamze, Susanne, Stillion, Richard J., Wong, Simon Y. C., Gordon, Siamon, Martinez-Pomares, Luisa

The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL+ cells). In naïve...

Degradation of basic protein in myelin by neutral proteases secreted by stimulated macrophages: A possible mechanism of inflammatory demyelination

Cammer, Wendy, Bloom, Barry R., Norton, William T., Gordon, Siamon

In inflammatory demyelinating diseases such as multiple sclerosis and experimental allergic encephalomyelitis, myelin destruction occurs in the vicinity of infiltrating mononuclear cells. The...

Cytokine Responses in Severe Acute Respiratory Syndrome Coronavirus-Infected Macrophages In Vitro: Possible Relevance to Pathogenesis

Cheung, Chung Y., Poon, Leo L. M., Ng, Iris H. Y., Luk, Winsie, Sia, Sin-Fun, Wu, Mavis H. S., ...

The pathogenesis of severe acute respiratory syndrome (SARS) remains unclear. Macrophages are key sentinel cells in the respiratory system, and it is therefore relevant to compare the responses of...

The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies of Aspergillus fumigatus

Steele, Chad, Rapaka, Rekha R, Metz, Allison, Pop, Shannon M, Williams, David L, Gordon, Siamon, ...

Alveolar macrophages represent a first-line innate host defense mechanism for clearing inhaled Aspergillus fumigatus from the lungs, yet contradictory data exist as to which alveolar macrophage...

Identification of Neisseria meningitidis Nonlipopolysaccharide Ligands for Class A Macrophage Scavenger Receptor by Using a Novel Assay‡

Peiser, Leanne, Makepeace, Katherine, Plüddemann, Annette, Savino, Silvana, Wright, J. Claire, Pizza, Mariagrazia, ...

Macrophages (Mφ) may play an important role in the pathogenesis of invasive meningococcal infection. Previously, we have shown that the class A Mφ scavenger receptor (SR-A) is a major nonopsonic...

Macrophage heterogeneity and tissue lipids

Gordon, Siamon

Macrophages are present as resident cells in adipose tissue, and blood monocytes are recruited in increased numbers to sites of lipid accumulation in atherosclerosis, a modified form of inflammation...

The potential for Toll-like receptors to collaborate with other innate immune receptors

Mukhopadhyay, Subhankar, Herre, Jurgen, Brown, Gordon D, Gordon, Siamon

Cells of the innate immune system express a large repertoire of germ-line encoded cell-surface glycoprotein receptors including Toll-like receptors (TLRs). TLRs recognize conserved motifs on microbes...

The use of human CD68 transcriptional regulatory sequences to direct high-level expression of class A scavenger receptor in macrophages in vitro and in vivo

Gough, Peter J, Gordon, Siamon, Greaves, David R

Macrophages (Mφ) play a key role in innate and acquired immunity. The study of Mφ biology has been hampered by the absence of suitable gene regulatory sequences for the overexpression of...

The Transmembrane Form of the CX3CL1 Chemokine Fractalkine Is Expressed Predominantly by Epithelial Cells in Vivo

Lucas, Andrew D., Chadwick, Nicholas, Warren, Bryan F., Jewell, Derek P., Gordon, Siamon, Powrie, Fiona, ...

Fractalkine (CX3CL1) is synthesized as a type I transmembrane protein. Its unique CX3C chemokine domain is attached to a 241-amino acid mucin stalk, a 19-amino acid transmembrane domain, and a...

RNA Interference Mutant Induction In Vivo Demonstrates the Essential Nature of Trypanosome Flagellar Function during Mammalian Infection▿

Griffiths, Samantha, Portman, Neil, Taylor, Philip R., Gordon, Siamon, Ginger, Michael L., Gull, Keith

We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell...

Impaired phagocytic mechanism in annexin 1 null macrophages

Yona, Simon, Heinsbroek, Sigrid E M, Peiser, Leanne, Gordon, Siamon, Perretti, Mauro, Flower, Roderick J

The role of the anti-inflammatory protein annexin-A1 (Anx-A1) in the phagocytic process has been investigated using a murine bone marrow culture-derived macrophage model from Anx-A1+/+ and...

Arylamine N-Acetyltransferase Is Required for Synthesis of Mycolic Acids and Complex Lipids in Mycobacterium bovis BCG and Represents a Novel Drug Target

Bhakta, Sanjib, Besra, Gurdyal S., Upton, Anna M., Parish, Tanya, Gibson, Kevin J.C., ...

Mycolic acids represent a major component of the unique cell wall of mycobacteria. Mycolic acid biosynthesis is inhibited by isoniazid, a key frontline antitubercular drug that is inactivated by...

The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance

Lin, Hsi-Hsien, Faunce, Douglas E., Stacey, Martin, Terajewicz, Ania, Nakamura, Takahiko, Zhang-Hoover, Jie, ...

We show that the mouse macrophage-restricted F4/80 protein is not required for the development and distribution of tissue macrophages but is involved in the generation of antigen-specific efferent...

A role for fungal β-glucans and their receptor Dectin-1 in the induction of autoimmune arthritis in genetically susceptible mice

Yoshitomi, Hiroyuki, Sakaguchi, Noriko, Kobayashi, Katsuya, Brown, Gordon D., Tagami, Tomoyuki, Sakihama, Toshiko, ...

A combination of genetic and environmental factors can cause autoimmune disease in animals. SKG mice, which are genetically prone to develop autoimmune arthritis, fail to develop the disease under a...

Developmental Regulation of Sialoadhesin (Sheep Erythrocyte Receptor), a Macrophage-Cell Interaction Molecule Expressed in Lymphohemopoietic Tissues

Morris, Lynn, Crocker, Paul R., Hill, Maxine, Gordon, Siamon

Stromal macrophages in lymphohemopoietic tissues express novel macrophagerestricted plasma membrane receptors involved in nonphagocytic interactions with other hemopoietic cells. One such receptor...

Dectin-1 Is A Major β-Glucan Receptor On Macrophages

Brown, Gordon D., Taylor, Philip R., Reid, Delyth M., Willment, Janet A., Williams, David L., Martinez-Pomares, Luisa, ...

Zymosan is a β-glucan– and mannan-rich particle that is widely used as a cellular activator for examining the numerous responses effected by phagocytes. The macrophage mannose receptor (MR) and...

Dectin-1 Mediates the Biological Effects of β-Glucans

Brown, Gordon D., Herre, Jurgen, Williams, David L., Willment, Janet A., Marshall, Andrew S. J., Gordon, Siamon

The ability of fungal-derived β-glucan particles to induce leukocyte activation and the production of inflammatory mediators, such as tumor necrosis factor (TNF)-α, is a well characterized...

Alveolar Macrophage–mediated Killing of Pneumocystis carinii f. sp. muris Involves Molecular Recognition by the Dectin-1 β-Glucan Receptor

Steele, Chad, Marrero, Luis, Swain, Steve, Harmsen, Allen G., Zheng, Mingquan, Brown, Gordon D., ...

Innate immune mechanisms against Pneumocystis carinii, a frequent cause of pneumonia in immunocompromised individuals, are not well understood. Using both real time polymerase chain reaction as a...

A Member of the Dendritic Cell Family That Enters B Cell Follicles and Stimulates Primary Antibody Responses Identified by a Mannose Receptor Fusion Protein

Berney, Claude, Herren, Suzanne, Power, Christine A., Gordon, Siamon, Martinez-Pomares, Luisa, Kosco-Vilbois, Marie H.

Dendritic cells (DCs) are known to activate naive T cells to become effective helper cells. In addition, recent evidence suggests that DCs may influence naive B cells during the initial priming of...

CCR6, a CC Chemokine Receptor that Interacts with Macrophage Inflammatory Protein 3α and Is Highly Expressed in Human Dendritic Cells

Greaves, David R., Wang, Wei, Dairaghi, Daniel J., Dieu, Marie Caroline, Saint-Vis, Blandine De, Franz-Bacon, Karin, ...

Dendritic cells initiate immune responses by ferrying antigen from the tissues to the lymphoid organs for presentation to lymphocytes. Little is known about the molecular mechanisms underlying this...

The Macrophage Scavenger Receptor Type A Is Expressed by Activated Macrophages and Protects the Host Against Lethal Endotoxic Shock

Haworth, Richard, Platt, Nick, Keshav, Satish, Hughes, Derralynn, Darley, Elisabeth, Suzuki, Hiroshi, ...

During gram-negative bacterial infections, lipopolysaccharide (LPS) stimulates primed macrophages (Mφ) to release inflammatory mediators such as tumor necrosis factor (TNF)-α, which can cause...

The Mannose Receptor Mediates Dengue Virus Infection of Macrophages

Miller, Joanna L, DeWet, Barend J. M, Martinez-Pomares, Luisa, Radcliffe, Catherine M, Dwek, Raymond A, Rudd, Pauline M, ...

Macrophages (MØ) and mononuclear phagocytes are major targets of infection by dengue virus (DV), a mosquito-borne flavivirus that can cause haemorrhagic fever in humans. To our knowledge, we show...

Generation of ligand-receptor alliances by "SEA" module-mediated cleavage of membrane-associated mucin proteins

Wreschner, Daniel H., McGuckin, Michael A., Williams, Stefanie J., Baruch, Amos, Yoeli, Merav, Ziv, Ravit, ...

A mechanism is described whereby one and the same gene can encode both a receptor protein as well as its specific ligand. Generation of this receptor–ligand partnership is effected by proteolytic...

Structure of the fungal β-glucan-binding immune receptor dectin-1: Implications for function

Brown, James, O'Callaghan, Chris A., Marshall, Andrew S.J., Gilbert, Robert J.C., Siebold, Christian, Gordon, Siamon, ...

The murine molecule dectin-1 (known as the β-glucan receptor in humans) is an immune cell surface receptor implicated in the immunological defense against fungal pathogens. Sequence analysis has...

Stage-Specific Sampling by Pattern Recognition Receptors during Candida albicans Phagocytosis

Heinsbroek, Sigrid E. M., Taylor, Philip R., Martinez, Fernando O., Martinez-Pomares, Luisa, Brown, Gordon D., Gordon, Siamon

Candida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be...