Typescript.
Thesis (B.S.)--Massachusetts Institute of Technology, Dept. of Physics, 1960.
Thesis (B.S.)--Massachusetts Institute of Technology, Dept. of Physics, 1960.
Thesis (B.S.)--Massachusetts Institute of Technology, Dept. of Physics, 1960.
A subunit of human nuclear RNase P has ATPase activity
Human nuclear RNase P purified from HeLa cells has ATPase activity. This activity is associated with one of the protein subunits of the enzyme, Rpp20. Thus, human nuclear RNase P, which contains...
Protein–protein interactions with subunits of human nuclear RNase P
Jiang, Taijiao, Altman, Sidney
A yeast two-hybrid system was used to analyze interactions among the protein subunits of human nuclear RNase P themselves and with other interacting partners encoded in a HeLa cell cDNA library....
Krummel, Daniel A. Pomeranz, Altman, Sidney
M1 RNA, the catalytic subunit of Escherichia coli RNase P, forms a secondary structure that includes five sequence variants of the tetraloop motif. Site-directed mutagenesis of the five tetraloops of...
Characterization of two scleroderma autoimmune antigens that copurify with human ribonuclease P
Eder, Paul S., Kekuda, Ramesh, Stolc, Viktor, Altman, Sidney
Human RNase P has been purified more than 2000-fold from HeLa cells. In addition to the RNA component, H1 RNA, polypeptides of molecular masses 14, 20, 25, 30, 38, and 40 kDa copurify with the enzyme...
Plehn-Dujowich, Debora, Altman, Sidney
The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide...
Stolc, Viktor, Katz, Alexander, Altman, Sidney
RPP2, an essential gene that encodes a 15.8-kDa protein subunit of nuclear RNase P, has been identified in the genome of Saccharomyces cerevisiae. Rpp2 was detected by sequence similarity with a...
Phenotypic conversion of drug-resistant bacteria to drug sensitivity
Guerrier-Takada, Cecilia, Salavati, Reza, Altman, Sidney
Plasmids that contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EGSs) have been introduced into strains of Escherichia coli harboring antibiotic...
Characterization of RNase P from Thermotoga maritima
Paul, Ralf, Lazarev, Denis, Altman, Sidney
The protein subunit of RNase P from a thermophilic bacterium, Thermotoga maritima, was overexpressed in and purified from Escherichia coli. The cloned protein was reconstituted with the RNA subunit...
McKinney, Jeffrey, Guerrier-Takada, Cecilia, Wesolowski, Donna, Altman, Sidney
Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In...
Jiang, Taijiao, Altman, Sidney
The processing of precursor tRNAs at their 5′ and 3′ termini is a fundamental event in the biosynthesis of tRNA. RNase P is generally responsible for endonucleolytic removal of a leader sequence...
Tightly Regulated Gene Expression System in Salmonella enterica Serovar Typhimurium
McKinney, Jeffrey, Guerrier-Takada, Cecilia, Galán, Jorge, Altman, Sidney
A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA...
An RNA affinity tag was incorporated into the RNA subunit of human nuclear RNase P. The tagged RNA assembled with the protein components of RNase P inside HeLa cells to generate an active enzyme....
Jovanovic, Milan, Sanchez, Ruth, Altman, Sidney, Gopalan, Venkat
RNase P is a ribonucleoprotein involved in tRNA biosynthesis in all living organisms. Bacterial RNase P is comprised of a catalytic RNA subunit and a lone protein cofactor which plays a supporting,...
Coordinate inhibition of expression of several genes for protein subunits of human nuclear RNase P
Kovrigina, Elizaveta, Wesolowski, Donna, Altman, Sidney
The deliberate inhibition of expression of one of the protein subunits (Rpp38) of human nuclear RNase P is achievable by using external guide sequence (EGS) technology. Both the protein product and...
A specific endoribonuclease, RNase P, affects gene expression of polycistronic operon mRNAs
The rnpA mutation, A49, in Escherichia coli reduces the level of RNase P at 43°C because of a temperature-sensitive mutation in C5 protein, the protein subunit of the enzyme. Microarray analysis...
A T4-Induced Endonuclease Which Attacks T4 DNA*
Altman, Sidney, Meselson, Matthew
A DNA endonucleolytic activity that introduces single-stranded breaks into native T4 DNA is found in T4-infected E. coli. This endonuclease attacks other double-stranded, but not single-stranded,...
The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human...
The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human...
McKinney, Jeffrey S., Zhang, Haifeng, Kubori, Tomoko, Galán, Jorge E., Altman, Sidney
The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs)...
RNase P cleaves transient structures in some riboswitches
Altman, Sidney, Wesolowski, Donna, Guerrier-Takada, Cecilia, Li, Yong
RNase P from Escherichia coli cleaves the coenzyme B12 riboswitch from E. coli and a similar one from Bacillus subtilis. The cleavage sites do not occur in any recognizable structure, as judged from...
Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P.
Guerrier-Takada, Cecilia, Eder, Paul S, Gopalan, Venkat, Altman, Sidney
In HeLa cells, ribonuclease P (RNase P), the tRNA processing enzyme consists of an RNA subunit (H1 RNA) associated with at least nine protein subunits, Rpp14, Rpp20, Rpp21, Rpp29 (hPop4), Rpp30,...
The effect of a single, temperature-sensitive mutation on global gene expression in Escherichia coli
LI, YONG, COLE, KYLE, ALTMAN, SIDNEY
High-density DNA microarrays have been used to explore the genomic profiling of gene expression of a defective Escherichia coli strain with a temperature-sensitive mutation in the protein component...
In search of RNase P RNA from microbial genomes
A simple procedure has been developed to quickly retrieve and validate the DNA sequence encoding the RNA subunit of ribonuclease P (RNase P RNA) from microbial genomes. RNase P RNA sequences were...
KOVRIGINA, ELIZAVETA, YANG, LI, PFUND, EMMANUEL, ALTMAN, SIDNEY
A regulatable promoter has been stably integrated into a human embryonic kidney cell line. The promoter is a pol III mouse promoter and is under the control of ponasterone A, an ecdysone inducer. The...
Context Effects on Nonsense Codon Suppression in ESCHERICHIA COLI
Feinstein, Sheldon I., Altman, Sidney
The influence of mRNA context on nonsense codon suppression has been studied by suppression measurements at one site in the Escherichia coli trpE gene and at two sites in the trpA gene. The ratio of...
A subunit of human nuclear RNase P has ATPase activity
Human nuclear RNase P purified from HeLa cells has ATPase activity. This activity is associated with one of the protein subunits of the enzyme, Rpp20. Thus, human nuclear RNase P, which contains...
Protein–protein interactions with subunits of human nuclear RNase P
Jiang, Taijiao, Altman, Sidney
A yeast two-hybrid system was used to analyze interactions among the protein subunits of human nuclear RNase P themselves and with other interacting partners encoded in a HeLa cell cDNA library....
Krummel, Daniel A. Pomeranz, Altman, Sidney
M1 RNA, the catalytic subunit of Escherichia coli RNase P, forms a secondary structure that includes five sequence variants of the tetraloop motif. Site-directed mutagenesis of the five tetraloops of...
Characterization of two scleroderma autoimmune antigens that copurify with human ribonuclease P
Eder, Paul S., Kekuda, Ramesh, Stolc, Viktor, Altman, Sidney
Human RNase P has been purified more than 2000-fold from HeLa cells. In addition to the RNA component, H1 RNA, polypeptides of molecular masses 14, 20, 25, 30, 38, and 40 kDa copurify with the enzyme...
Plehn-Dujowich, Debora, Altman, Sidney
The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide...
Stolc, Viktor, Katz, Alexander, Altman, Sidney
RPP2, an essential gene that encodes a 15.8-kDa protein subunit of nuclear RNase P, has been identified in the genome of Saccharomyces cerevisiae. Rpp2 was detected by sequence similarity with a...
Phenotypic conversion of drug-resistant bacteria to drug sensitivity
Guerrier-Takada, Cecilia, Salavati, Reza, Altman, Sidney
Plasmids that contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EGSs) have been introduced into strains of Escherichia coli harboring antibiotic...
Characterization of RNase P from Thermotoga maritima
Paul, Ralf, Lazarev, Denis, Altman, Sidney
The protein subunit of RNase P from a thermophilic bacterium, Thermotoga maritima, was overexpressed in and purified from Escherichia coli. The cloned protein was reconstituted with the RNA subunit...
McKinney, Jeffrey, Guerrier-Takada, Cecilia, Wesolowski, Donna, Altman, Sidney
Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In...
Jiang, Taijiao, Altman, Sidney
The processing of precursor tRNAs at their 5′ and 3′ termini is a fundamental event in the biosynthesis of tRNA. RNase P is generally responsible for endonucleolytic removal of a leader sequence...
Tightly Regulated Gene Expression System in Salmonella enterica Serovar Typhimurium
McKinney, Jeffrey, Guerrier-Takada, Cecilia, Galán, Jorge, Altman, Sidney
A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA...
An RNA affinity tag was incorporated into the RNA subunit of human nuclear RNase P. The tagged RNA assembled with the protein components of RNase P inside HeLa cells to generate an active enzyme....
Jovanovic, Milan, Sanchez, Ruth, Altman, Sidney, Gopalan, Venkat
RNase P is a ribonucleoprotein involved in tRNA biosynthesis in all living organisms. Bacterial RNase P is comprised of a catalytic RNA subunit and a lone protein cofactor which plays a supporting,...
Coordinate inhibition of expression of several genes for protein subunits of human nuclear RNase P
Kovrigina, Elizaveta, Wesolowski, Donna, Altman, Sidney
The deliberate inhibition of expression of one of the protein subunits (Rpp38) of human nuclear RNase P is achievable by using external guide sequence (EGS) technology. Both the protein product and...
A specific endoribonuclease, RNase P, affects gene expression of polycistronic operon mRNAs
The rnpA mutation, A49, in Escherichia coli reduces the level of RNase P at 43°C because of a temperature-sensitive mutation in C5 protein, the protein subunit of the enzyme. Microarray analysis...
A T4-Induced Endonuclease Which Attacks T4 DNA*
Altman, Sidney, Meselson, Matthew
A DNA endonucleolytic activity that introduces single-stranded breaks into native T4 DNA is found in T4-infected E. coli. This endonuclease attacks other double-stranded, but not single-stranded,...
The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human...
The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human...
McKinney, Jeffrey S., Zhang, Haifeng, Kubori, Tomoko, Galán, Jorge E., Altman, Sidney
The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs)...
RNase P cleaves transient structures in some riboswitches
Altman, Sidney, Wesolowski, Donna, Guerrier-Takada, Cecilia, Li, Yong
RNase P from Escherichia coli cleaves the coenzyme B12 riboswitch from E. coli and a similar one from Bacillus subtilis. The cleavage sites do not occur in any recognizable structure, as judged from...
Context Effects on Nonsense Codon Suppression in ESCHERICHIA COLI
Feinstein, Sheldon I., Altman, Sidney
The influence of mRNA context on nonsense codon suppression has been studied by suppression measurements at one site in the Escherichia coli trpE gene and at two sites in the trpA gene. The ratio of...
Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P.
Guerrier-Takada, Cecilia, Eder, Paul S, Gopalan, Venkat, Altman, Sidney
In HeLa cells, ribonuclease P (RNase P), the tRNA processing enzyme consists of an RNA subunit (H1 RNA) associated with at least nine protein subunits, Rpp14, Rpp20, Rpp21, Rpp29 (hPop4), Rpp30,...
The effect of a single, temperature-sensitive mutation on global gene expression in Escherichia coli
LI, YONG, COLE, KYLE, ALTMAN, SIDNEY
High-density DNA microarrays have been used to explore the genomic profiling of gene expression of a defective Escherichia coli strain with a temperature-sensitive mutation in the protein component...
In search of RNase P RNA from microbial genomes
A simple procedure has been developed to quickly retrieve and validate the DNA sequence encoding the RNA subunit of ribonuclease P (RNase P RNA) from microbial genomes. RNase P RNA sequences were...
KOVRIGINA, ELIZAVETA, YANG, LI, PFUND, EMMANUEL, ALTMAN, SIDNEY
A regulatable promoter has been stably integrated into a human embryonic kidney cell line. The promoter is a pol III mouse promoter and is under the control of ponasterone A, an ecdysone inducer. The...
A noncoding RNA in Saccharomyces cerevisiae is an RNase P substrate
Ribonuclease P (RNase P) is involved in regulation of noncoding RNA (ncRNA) expression in Saccharomyces cerevisiae. A hidden-in-reading-frame antisense-1 (HRA1) RNA in S. cerevisiae, which belongs to...
Ko, Jae-hyeong, Altman, Sidney
OLE (ornate, large, and extremophilic) RNA is a noncoding RNA that is found in several extremophilic bacteria, including Bacillus halodurans. The function of OLE RNA has not been clarified. In this...
Jarrous, Nayef, Wolenski, Joseph S., Wesolowski, Donna, Lee, Christopher, Altman, Sidney
The precise location of the tRNA processing ribonucleoprotein ribonuclease P (RNase P) and the mechanism of its intranuclear distribution have not been completely delineated. We show that three...
Lundblad, Eirik W., Xiao, Gaoping, Ko, Jae-hyeong, Altman, Sidney
A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are...
Xiao, Gaoping, Lundblad, Eirik W., Izadjoo, Mina, Altman, Sidney
External guide sequences (EGSs) have successfully been used to inhibit expression of target genes at the post-transcriptional level in both prokaryotes and eukaryotes. We previously reported that EGS...