Discovery of common marburgvirus protective epitopes in a BALB/c mouse model (2009)
Kalina, Warren V, Warfield, Kelly L, Olinger, Gene G, Bavari, Sina
Abstract Background Marburg virus (MARV) causes acute hemorrhagic fever that is often lethal, and no licensed vaccines are available for preventing this deadly viral infection. The immune mechanisms...
Development of a model for marburgvirus based on severe-combined immunodeficiency mice (2007)
Warfield, Kelly L, Alves, Derron A, Bradfute, Steven B, Reed, Daniel K, VanTongeren, Sean, Kalina, Warren V, ...
Abstract The filoviruses, Ebola (EBOV) and Marburg (MARV), cause a lethal hemorrhagic fever. Human isolates of MARV are not lethal to immmunocompetent adult mice and, to date, there are no reports of...
Burnett, James C., Opsenica, Dejan, Sriraghavan, Kamaraj, Panchal, Rekha G., Ruthel, Gordon, Hermone, Ann R., ...
We previously identified structurally diverse small molecule (non-peptidic) inhibitors (SMNPIs) of the botulinum neurotoxin serotype A (BoNT/A) light chain (LC). Of these, several (including...
Kelly L. Warfield, Dana L. Swenson, Gene G. Olinger, Donald K. Nichols, William D. Pratt, Robert Blouch, ...
The filoviruses Marburg virus and Ebola virus (EBOV) quickly outpace host immune responses and cause hemorrhagic fever, resulting in case fatality rates as high as 90% in humans and nearly 100% in...
Ribot, Wilson J., Panchal, Rekha G., Brittingham, Katherine C., Ruthel, Gordon, Kenny, Tara A., Lane, Douglas, ...
Alveolar macrophages (AM) are very important for pulmonary innate immune responses against invading inhaled pathogens because they directly kill the organisms and initiate a cascade of innate and...
Burnett, James C., Schmidt, James J., McGrath, Connor F., Nguyen, Tam L., Hermone, Ann R., Panchal, Rekha G., ...
Botulinum neurotoxins (BoNTs) are the most potent of the known biological toxins, and consequently are listed as category A biowarfare agents. Currently, the only treatments against BoNTs include...
Novel Small Molecule Inhibitors of Botulinum Neurotoxin a Metalloprotease Activity (2003)
Burnett, James C., Schmidt, James J., Stafford, Robert G., Panchal, Rekha G., Nguyen, Tam L., Hermone, Ann R., ...
Botulinum neurotoxins (BoNTs) are among the most lethal biological substances to have been weaponized and are listed as biodefense category A agents. Currently, no small molecule (non-peptidic)...
Time-Lapse Confocal Imaging of Development of Bacillus anthracis in Macrophages (1998)
Ruthel, Gordon, Ribot, Wilson J., Bavari, Sina, Hoover, Timothy A.
Macrophages attempt to battle infection with Bacillus anthracis spores by phagocytosis of the spores. However, it is believed that B. anthracis spores may survive phagocytosis and may actually use...
Carra, John H., Welcher, Brent C., Schokman, Rowene D., David, Chella S., Bavari, Sina
The influence of mutationally induced changes in protein folding on development of effective neutralizing antibodies during vaccination remains largely unexplored. In this study, we probed how...
Association of Ebola Virus Matrix Protein VP40 with Microtubules (1998)
Ruthel, Gordon, Demmin, Gretchen L., Kallstrom, George, Javid, Melodi P., Badie, Shirin S., Will, Amy B., ...
Viruses exploit a variety of cellular components to complete their life cycles, and it has become increasingly clear that use of host cell microtubules is a vital part of the infection process for...
Roy, Chad J., Warfield, Kelly L., Welcher, Brent C., Gonzales, Raoul F., Larsen, Tom, Hanson, Julie, ...
Staphylococcal enterotoxins (SEs) belong to a large group of bacterial exotoxins that cause severe immunopathologies, especially when delivered as an aerosol. SEs elicit the release of lethal amounts...
Swenson, Dana L., Warfield, Kelly L., Negley, Diane L., Schmaljohn, Alan, Aman, M. J., Bavari, Sina
A safe and effective pan-filovirus vaccine is highly desirable since the filoviruses Ebola virus (EBOV) and Marburg virus (MARV) cause highly lethal disease typified by unimpeded viral replication...
Analysis of Ebola Virus and VLP Release Using an Immunocapture Assay (1998)
Kallstrom, George, Warfield, Kelly L., Swenson, Dana L., Mort, Shannon, Panchal, Rekha G., Ruthel, Gordon, ...
Ebola virus (EBOV), an emerging pathogen, is the causative agent of a rapidly progressive hemorrhagic fever with high mortality rates. There are currently no approved vaccines or treatments available...
Burnett, James C., Schmidt, James J., McGrath, Connor F., Nguyen, Tam L., Hermone, Ann R., Panchal, Rekha G., ...
Botulinum neurotoxins (BoNTs) are the most potent of the known biological toxins, and consequently are listed as category A biowarfare agents. Currently, the only treatments against BoNTs include...
Nguyen, Tam L., Schoehn, Guy, Weissenhorn, Winfried, Hermone, Ann R., Burnett, James C., Panchal, Rekha G., ...
The matrix protein VP40 is an indispensable component of viral assembly and budding by the Ebola virus. VP40 is a monomer in solution, but can fold into hexameric and octameric states, two oligomeric...
The Evolving Field of Biodefence: Therapeutic Developments and Diagnostics (1998)
Burnett, James C., Henchal, Erik A., Schmaljohn, Alan L., Bavari, Sina
The threat of bioterrorism and the potential use of biological weapons against both military and civilian populations have become major concerns for governments around the world. For perspective, in...
Filovirus-Like Particles as Vaccines and Discovery Tools (1998)
Warfield, Kelly L., Swenson, Dana L., Demmin, Gretchen L., Bavari, Sina
Ebola and Marburg viruses are members of the family Filoviridae, which cause severe hemorrhagic fevers in humans. Filovirus outbreaks have been sporadic, with mortality rates currently ranging from...
Pless, Dorothy D., Ruthel, Gordon, Reinke, Emily K., Ulrich, Robert G., Bavari, Sina
Bacterial superantigen intoxication causes massive overactivation of T cells, which can result in potentially lethal toxic shock. Superantigens fall into two groups: superantigens such as...
Anthrax Biosensor, Protective Antigen Ion Channel Asymmetric Blockade (1998)
Halverson, Kelly M., Panchal, Rekha G., Nguyen, Tam L., Gussio, Rick, Little, Stephen F., Misakian, Martin, ...
Bacillus anthracis secretes three proteins (protective antigen, PA; lethal factor, LF; and edema factor, EF) that are the basis for anthrax infection. We present electrophysiological measurements...
Warfield, Kelly L., Swenson, Dana L., Olinger, Gene G., Nicholas, Donald K., Pratt, William D., Blouch, Robert, ...
The filoviruses Marburg virus and Ebola virus (EBOV) quickly outpace host immune responses and cause hemorrhagic fever, resulting in case fatality rates as high as 90% in humans and nearly 100% in...
Enterlein, Sven, Warfield, Kelly L., Swenson, Dana L., Stein, David A., Smith, Jeffrey L., Gamble, C. S., ...
Phosphorodiamidate morpholino oligomers (PMO) are a class of uncharged single-stranded DNA analogs modified such that each subunit includes a phosphorodiamidate linkage and morpholine ring. PMO...
Lactobacilli Activate Human Dendritic Cells that Skew T Cells Toward T Helper 1 Polarization (1998)
Mohamadzadeh, Mansour, Olson, Scott, Kalina, Warren V., Ruthel, Gordon, Demmin, Gretchen L., Warfield, Kelly L., ...
Professional antigen-presenting dendritic cells (DCs) are critical in regulating T cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human...
Kuhn, Jens H., Radoshitzky, Sheli R., Guth, Alexander C., Warfield, Kelly L., Li, Wenhui, Vincent, Martin J., ...
The GP(sub 1,2) envelope glycoproteins of filoviruses (marburg- and ebolaviruses) mediate cell-surface attachment, membrane fusion, and entry into permissive cells. Here we show that a 151-amino acid...
Fuller, Claudette L., Brittingham, Katherine C., Hepburn, Matthew J., Martin, James W., Petitt, Patricia L., Pittman, Phillip R., ...
Francisella tularensis is the etiologic agent of the zoonotic disease, tularemia. An inoculum as small as 10 bacteria can cause a flulike disease with substantial morbidity and mortality among...
An ELISA for Studying Ebola Virus / VLP Release (1998)
Kallstrom, George, Aman, M. J., Bavari, Sina
Partial contents: Filovirus Background, ELISA setup and optimization, Effects of Ebola proteins on VLP release, Effects of VP40 mutants on VLP release, Therapeutic compound screening.
I̲n̲ v̲i̲t̲r̲o̲ immune modulation by anti-cholinesterase insecticides /--by Sina Bavari. (1991)
Typescript (photocopy).
BAVARI, SINA, CASALE, GEORGE P., GOLD, ROGER E., VITZTHUM, EDWARD F.
Modulation of Interieukin-2-Driven Proliferation of Human Large Granular Lymphocytes by Carbaryl, an Anticholinesterase Insecticide. Bavari, S., Casale, G. P., Gold, R. E., and Vitzthum, E. F....
CASALE, GEORGE P, BAVARI, SINA, CONNOLLY, JOHN J
Inhibition of Human Serum Complement Activity by Diisopropylfluorophosphate and Selected Anticholinesterase Insecticides. CASALE, G. P., BAVARI, S., AND CONNOLLY, J. J. (1989). Fundam. Appl. Toxicol....
LeClaire, Ross D., Bavari, Sina
Bacterial superantigens (BSAgs) cause massive stimulation of the immune system and are associated with various pathologies and diseases. To address the role of antibodies in protection against BSAgs,...
High-Affinity, Protective Antibodies to the Binding Domain of Botulinum Neurotoxin Type A
Pless, Dorothy D., Torres, Edna R., Reinke, Emily K., Bavari, Sina
Monoclonal antibodies (MAbs) were prepared against the putative binding domain of botulinum neurotoxin A (BoNT/A), a nontoxic 50-kDa fragment. Initially, all fusion products were screened against the...
Erwin, James L., DaSilva, Luis M., Bavari, Sina, Little, Stephen F., Friedlander, Arthur M., Chanh, Tran C.
We present evidence that Bacillus anthracis lethal toxin (LT) suppresses rather than induces proinflammatory cytokine production in macrophages. Suppression is observed with extremely low levels of...
Protection against Bacterial Superantigen Staphylococcal Enterotoxin B by Passive Vaccination
LeClaire, Ross D., Hunt, Robert E., Bavari, Sina
We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken...
Ebola virus-like particles protect from lethal Ebola virus infection
Warfield, Kelly L., Bosio, Catharine M., Welcher, Brent C., Deal, Emily M., Mohamadzadeh, Mansour, Schmaljohn, Alan, ...
The filovirus Ebola causes hemorrhagic fever with 70–80% human mortality. High case-fatality rates, as well as known aerosol infectivity, make Ebola virus a potential global health threat and...
In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding
Panchal, Rekha G., Ruthel, Gordon, Kenny, Tara A., Kallstrom, George H., Lane, Douglas, Badie, Shirin S., ...
The matrix protein VP40 plays a critical role in Ebola virus assembly and budding, a process that utilizes specialized membrane domains known as lipid rafts. Previous studies with purified protein...
Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization
Mohamadzadeh, Mansour, Olson, Scott, Kalina, Warren V., Ruthel, Gordon, Demmin, Gretchen L., Warfield, Kelly L., ...
Professional antigen-presenting dendritic cells (DCs) are critical in regulating T cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human...
Association of Ebola Virus Matrix Protein VP40 with Microtubules
Ruthel, Gordon, Demmin, Gretchen L., Kallstrom, George, Javid, Melodi P., Badie, Shirin S., Will, Amy B., ...
Viruses exploit a variety of cellular components to complete their life cycles, and it has become increasingly clear that use of host cell microtubules is a vital part of the infection process for...
Roy, Chad J., Warfield, Kelly L., Welcher, Brent C., Gonzales, Raoul F., Larsen, Tom, Hanson, Julie, ...
Staphylococcal enterotoxins (SEs) belong to a large group of bacterial exotoxins that cause severe immunopathologies, especially when delivered as an aerosol. SEs elicit the release of lethal amounts...
Pless, Dorothy D., Ruthel, Gordon, Reinke, Emily K., Ulrich, Robert G., Bavari, Sina
Bacterial superantigen intoxication causes massive overactivation of T cells, which can result in potentially lethal toxic shock. Superantigens fall into two groups: superantigens such as...
Warfield, Kelly L, Swenson, Dana L, Olinger, Gene G, Nichols, Donald K, Pratt, William D, Blouch, Robert, ...
The filoviruses Marburg virus and Ebola virus (EBOV) quickly outpace host immune responses and cause hemorrhagic fever, resulting in case fatality rates as high as 90% in humans and nearly 100% in...
VP35 Knockdown Inhibits Ebola Virus Amplification and Protects against Lethal Infection in Mice
Enterlein, Sven, Warfield, Kelly L., Swenson, Dana L., Stein, David A., Smith, Jeffery L., Gamble, C. Scott, ...
Phosphorodiamidate morpholino oligomers (PMO) are a class of uncharged single-stranded DNA analogs modified such that each subunit includes a phosphorodiamidate linkage and morpholine ring. PMO...
LeClaire, Ross D., Bavari, Sina
Bacterial superantigens (BSAgs) cause massive stimulation of the immune system and are associated with various pathologies and diseases. To address the role of antibodies in protection against BSAgs,...
High-Affinity, Protective Antibodies to the Binding Domain of Botulinum Neurotoxin Type A
Pless, Dorothy D., Torres, Edna R., Reinke, Emily K., Bavari, Sina
Monoclonal antibodies (MAbs) were prepared against the putative binding domain of botulinum neurotoxin A (BoNT/A), a nontoxic 50-kDa fragment. Initially, all fusion products were screened against the...
Erwin, James L., DaSilva, Luis M., Bavari, Sina, Little, Stephen F., Friedlander, Arthur M., Chanh, Tran C.
We present evidence that Bacillus anthracis lethal toxin (LT) suppresses rather than induces proinflammatory cytokine production in macrophages. Suppression is observed with extremely low levels of...
Protection against Bacterial Superantigen Staphylococcal Enterotoxin B by Passive Vaccination
LeClaire, Ross D., Hunt, Robert E., Bavari, Sina
We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken...
Ebola virus-like particles protect from lethal Ebola virus infection
Warfield, Kelly L., Bosio, Catharine M., Welcher, Brent C., Deal, Emily M., Mohamadzadeh, Mansour, Schmaljohn, Alan, ...
The filovirus Ebola causes hemorrhagic fever with 70–80% human mortality. High case-fatality rates, as well as known aerosol infectivity, make Ebola virus a potential global health threat and...
In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding
Panchal, Rekha G., Ruthel, Gordon, Kenny, Tara A., Kallstrom, George H., Lane, Douglas, Badie, Shirin S., ...
The matrix protein VP40 plays a critical role in Ebola virus assembly and budding, a process that utilizes specialized membrane domains known as lipid rafts. Previous studies with purified protein...
Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization
Mohamadzadeh, Mansour, Olson, Scott, Kalina, Warren V., Ruthel, Gordon, Demmin, Gretchen L., Warfield, Kelly L., ...
Professional antigen-presenting dendritic cells (DCs) are critical in regulating T cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human...
Association of Ebola Virus Matrix Protein VP40 with Microtubules
Ruthel, Gordon, Demmin, Gretchen L., Kallstrom, George, Javid, Melodi P., Badie, Shirin S., Will, Amy B., ...
Viruses exploit a variety of cellular components to complete their life cycles, and it has become increasingly clear that use of host cell microtubules is a vital part of the infection process for...
Roy, Chad J., Warfield, Kelly L., Welcher, Brent C., Gonzales, Raoul F., Larsen, Tom, Hanson, Julie, ...
Staphylococcal enterotoxins (SEs) belong to a large group of bacterial exotoxins that cause severe immunopathologies, especially when delivered as an aerosol. SEs elicit the release of lethal amounts...
Pless, Dorothy D., Ruthel, Gordon, Reinke, Emily K., Ulrich, Robert G., Bavari, Sina
Bacterial superantigen intoxication causes massive overactivation of T cells, which can result in potentially lethal toxic shock. Superantigens fall into two groups: superantigens such as...
Warfield, Kelly L, Swenson, Dana L, Olinger, Gene G, Nichols, Donald K, Pratt, William D, Blouch, Robert, ...
The filoviruses Marburg virus and Ebola virus (EBOV) quickly outpace host immune responses and cause hemorrhagic fever, resulting in case fatality rates as high as 90% in humans and nearly 100% in...
VP35 Knockdown Inhibits Ebola Virus Amplification and Protects against Lethal Infection in Mice
Enterlein, Sven, Warfield, Kelly L., Swenson, Dana L., Stein, David A., Smith, Jeffery L., Gamble, C. Scott, ...
Phosphorodiamidate morpholino oligomers (PMO) are a class of uncharged single-stranded DNA analogs modified such that each subunit includes a phosphorodiamidate linkage and morpholine ring. PMO...
Ribot, Wilson J., Panchal, Rekha G., Brittingham, Katherine C., Ruthel, Gordon, Kenny, Tara A., Lane, Douglas, ...
Alveolar macrophages (AM) are very important for pulmonary innate immune responses against invading inhaled pathogens because they directly kill the organisms and initiate a cascade of innate and...
Development of a model for marburgvirus based on severe-combined immunodeficiency mice
Warfield, Kelly L, Alves, Derron A, Bradfute, Steven B, Reed, Daniel K, VanTongeren, Sean, Kalina, Warren V, ...
The filoviruses, Ebola (EBOV) and Marburg (MARV), cause a lethal hemorrhagic fever. Human isolates of MARV are not lethal to immmunocompetent adult mice and, to date, there are no reports of a...
Role of Natural Killer Cells in Innate Protection against Lethal Ebola Virus Infection
Warfield, Kelly L., Perkins, Jeremy G., Swenson, Dana L., Deal, Emily M., Bosio, Catharine M., Aman, M. Javad, ...
Ebola virus is a highly lethal human pathogen and is rapidly driving many wild primate populations toward extinction. Several lines of evidence suggest that innate, nonspecific host factors are...
Lipid Raft Microdomains: A Gateway for Compartmentalized Trafficking of Ebola and Marburg Viruses〉
Bavari, Sina, Bosio, Catharine M., Wiegand, Elizabeth, Ruthel, Gordon, Will, Amy B., Geisbert, Thomas W., ...
Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the...
Sizing the Bacillus anthracis PA63 Channel with Nonelectrolyte Poly(Ethylene Glycols)
Nablo, Brian J., Halverson, Kelly M., Robertson, Joseph W. F., Nguyen, Tam L., Panchal, Rekha G., Gussio, Rick, ...
Nonelectrolyte polymers of poly(ethylene glycol) (PEG) were used to estimate the diameter of the ion channel formed by the Bacillus anthracis protective antigen 63 (PA63). Based on the ability of...
Discovery of common marburgvirus protective epitopes in a BALB/c mouse model
Kalina, Warren V, Warfield, Kelly L, Olinger, Gene G, Bavari, Sina
Swenson, Dana L., Warfield, Kelly L., Warren, Travis K., Lovejoy, Candace, Hassinger, Jed N., Ruthel, Gordon, ...
Phosphorodiamidate morpholino oligomers (PMOs) are uncharged nucleic acid-like molecules designed to inactivate the expression of specific genes via the antisense-based steric hindrance of mRNA...