Das, Mili, Kobayashi, Masanori, Yamada, Yusuke, Sreeramulu, Sridhar, Ramakrishnan, C, Wakatsuki, Soichi, ...
Disulfide bonds play an important role in protein stability and function. Here, we describe a general procedure for generating disulfide-linked dimers and multimers of proteins of known crystal...
Das, Mili, Kobayashi, Masanori, Yamada, Yusuke, Sreeramulu, Sridhar, Ramakrishnan, C, Wakatsuki, Soichi, ...
Disulfide bonds play an important role in protein stability and function. Here, we describe a general procedure for generating disulfide-linked dimers and multimers of proteins of known crystal...
Submitted to the Department of Chemistry.
Crystal structure of troponin C in complex with troponin I fragment at 2.3-Å resolution
Vassylyev, Dmitry G., Takeda, Soichi, Wakatsuki, Soichi, Maeda, Kayo, Maéda, Yuichiro
Troponin (Tn), the complex of three subunits (TnC, TnI, and TnT), plays a key role in Ca2+-dependent regulation of muscle contraction. To elucidate the interactions between the Tn subunits and the...
Chang, Chung-I, Ihara, Kentaro, Chelliah, Yogarany, Mengin-Lecreulx, Dominique, Wakatsuki, Soichi, Deisenhofer, Johann
The peptidoglycan-recognition protein LCa (PGRP-LCa) is a transmembrane receptor required for activation of the Drosophila immune deficiency pathway by monomeric Gram-negative peptidoglycan. We have...
Structural Basis of Transcription Inhibition by Antibiotic Streptolydigin
Temiakov, Dmitry, Zenkin, Nikolay, Vassylyeva, Marina N., Perederina, Anna, Tahirov, Tahir H., Kashkina, Ekaterina, ...
Streptolydigin (Stl) is a potent inhibitor of bacterial RNA polymerases (RNAPs). The 2.4 Å resolution structure of the Thermus thermophilus RNAP-Stl complex showed that, in full agreement with the...
Artsimovitch, Irina, Vassylyeva, Marina N., Svetlov, Dmitri, Svetlov, Vladimir, Perederina, Anna, Igarashi, Noriyuki, ...
Rifamycins, the clinically important antibiotics, target bacterial RNA polymerase (RNAP). A proposed mechanism in which rifamycins sterically block the extension of nascent RNA beyond three...
Crystal structure of troponin C in complex with troponin I fragment at 2.3-Å resolution
Vassylyev, Dmitry G., Takeda, Soichi, Wakatsuki, Soichi, Maeda, Kayo, Maéda, Yuichiro
Troponin (Tn), the complex of three subunits (TnC, TnI, and TnT), plays a key role in Ca2+-dependent regulation of muscle contraction. To elucidate the interactions between the Tn subunits and the...
Chang, Chung-I, Ihara, Kentaro, Chelliah, Yogarany, Mengin-Lecreulx, Dominique, Wakatsuki, Soichi, Deisenhofer, Johann
The peptidoglycan-recognition protein LCa (PGRP-LCa) is a transmembrane receptor required for activation of the Drosophila immune deficiency pathway by monomeric Gram-negative peptidoglycan. We have...
Shiba, Tomoo, Koga, Hiroshi, Shin, Hye-Won, Kawasaki, Masato, Kato, Ryuichi, Nakayama, Kazuhisa, ...
Family of Rab11-interacting protein (FIP)3/Arfophlin-1 and FIP4/Arfophilin-2 are dual effectors for Rab11 and ADP ribosylation factor (ARF)5/ARF6, which are involved in membrane delivery from...
Kudo, Norio, Kumagai, Keigo, Tomishige, Nario, Yamaji, Toshiyuki, Wakatsuki, Soichi, Nishijima, Masahiro, ...
In mammalian cells, ceramide is synthesized in the endoplasmic reticulum and transferred to the Golgi apparatus for conversion to sphingomyelin. Ceramide transport occurs in a nonvesicular manner and...
X-ray beam stabilization at BL-17A, the protein microcrystallography beamline of the Photon Factory
Igarashi, Noriyuki, Ikuta, Kazuyuki, Miyoshi, Toshinobu, Matsugaki, Naohiro, Yamada, Yusuke, Yousef, Mohammad S., ...
BL-17A is a new structural biology beamline at the Photon Factory, dedicated to protein crystallography of microcrystals. Here the X-ray beam stabilization techniques used at BL-17A are described.
Hiraki, Masahiko, Watanabe, Shokei, PHonda, Nobuo, Yamada, Yusuke, Matsugaki, Naohiro, Igarashi, Noriyuki, ...
Sample-exchange robots with a double-tongs system that could exchange samples within 10 s have been developed.
Yamada, Yusuke, PHonda, Nobuo, Matsugaki, Naohiro, Igarashi, Noriyuki, Hiraki, Masahiko, Wakatsuki, Soichi
At the Photon Factory macromolecular crystallography beamlines, two new functions, remote monitoring and diffraction image evaluation, have been developed and installed on the beamline controlling...
Development of an X-ray HARP–FEA detector system for high-throughput protein crystallography
Miyoshi, Toshinobu, Igarashi, Noriyuki, Matsugaki, Naohiro, Yamada, Yusuke, Hirano, Keiichi, Hyodo, Kazuyuki, ...
A new detector system for protein crystallography based on an X-ray HARP–FEA is presented.
Murakami, Kenji, Stewart, Murray, Nozawa, Kayo, Tomii, Kumiko, Kudou, Norio, Igarashi, Noriyuki, ...
Head-to-tail polymerization of tropomyosin is crucial for its actin binding, function in actin filament assembly, and the regulation of actin-myosin contraction. Here, we describe the 2.1 Å...