A simplified bioprocess for human alpha-fetoprotein production from inclusion bodies (2007)
A simple and effective Escherichia coli (E. coli) bioprocess is demonstrated for the preparation of recombinant human alpha-fetoprotein (rhAFP), a pharmaceutically promising protein that has...
Controlled oxidative protein refolding using an ion-exchange column
Langenhof, M, Leong, SSJ, Pattenden, LK, Middelberg, APJ
Column-based refolding of complex and highly disulfide-bonded proteins simplifies protein renaturation at both preparative and process scale by integrating and automating a number of operations...
Alpha-fetoprotein (AFP) is a commercially important polypeptide with important diagnostic. physiological and immunomodulatory functions. Previous studies into the refolding of this macromolecule are...
The refolding of different alpha-fetoprotein variants
The effect of glycosylation on AFP foldability was investigated by parallel quantitative and qualitative analyses of the refolding of glycosylated and nonglycosylated AFP variants. Both variants were...