Joyce, Elizabeth A, Popper, Stephen J, Falkow, Stanley
Abstract Background We employed DNA microarray technology to investigate the host response to Streptococcus pneumoniae in a mouse model of asymptomatic carriage. Over a period of six weeks, we...
Anne Mueller, Pauline Chu, A Chu, Michael F. Dixon, Donna M. Bouley, Adrian Lee, ...
Gastric B-cell lymphoma of mucosa-associated lymphoid tissue type is closely linked to chronic Helicobacter pylori infection. Most clinical and histopathological features of the tumor can be...
Charles C Kim, Charles C Kim, Stanley Falkow, Stanley Falkow
Background: Genes that are determined to be significantly differentially regulated in microarray analyses often appear to have functional commonalities, such as being components of the same...
BEHAVIOR OF COLIPHAGE LAMBDA IN HYBRIDS BETWEEN ESCHERICHIA COLI AND SALMONELLA (2005)
Baron, L. S., Penido, Elisa, Ryman, I. R., Falkow, Stanley
Salmonella typhosa hybrids able to adsorb lambda were obtained by mating S. typhosa recipients with Escherichia coli K-12 donors. After adsorption of wild-type lambda to these S. typhosa hybrids, no...
Significance analysis of lexical bias in microarray data (2003)
Kim, Charles C, Falkow, Stanley
Abstract Background Genes that are determined to be significantly differentially regulated in microarray analyses often appear to have functional commonalities, such as being components of the same...
Infectious Multiple Drug Resistance in the Enterobacteriaceae (2003)
The applicability and limitations of examining clinical specimins with DNA hybridization technique for gnes encoding enterotoxins was examined using 24 heat-labile and heat-stable (LT+ST+), 17...
Infectious Multiple Drug Resistance in the Enterobacteriaceae (2003)
A new method for the detection of plasmid DNA in clinical isolates has been developed. The method requires only small culture volumes and can detect plasmid genomes ranging from 1 x 10 to the 6th...
Infectious Multiple Drug Resistance in the Enterobacteriaceae (2003)
Current methods for the detection and isolation of enterotoxigenic e. coli (ETEC) are unsuitable for assaying large numbers of isolates. An alternative method is described which is based on detection...
Molecular Basis of Pathogenicity in Enteric Bacteria (2003)
We have focused upon the genetic and molecular basis by which pathogenic microorganisms penetrate eucaryotic cells. Our studies show that the pathogenic Versinia share at least two distinct...
Molecular Basis of Pathogenicity in Enteric Bacteria (2003)
A new quantitative assay for the determination of the E. coli heat- stable enterotoxin has been developed which is about 10-fold more sensitive than existing methods. A strategy for the development...
Infectious Multiple Drug Resistance in the Enterobacteriaceae (2003)
A new quantitative assay for the determination of E. coli heat-stable enterotoxin has been developed which is about 10-fold more sensitive than existing methods. A strategy for the development of...
Infectious Multiple Drug Resistance in the Enterobacteriaceae (2003)
The antibiotic resistance gene of R plasmids which encodes for ampicillin resistance resides upon a 3.7 x 3700000 dalton sequence of DNA, TnA, which can migrate (translocate) from plasmid to plasmid....
Infectious Multiple Drug Resistance in the Enterobacteriaceae (2003)
Colicin V is a protein elaborated by a variety of plasmids called collectively, ColV. It has been shown that ColV plasmids are far more likely to be found in E. coli strains isolated from...
Improved analytical methods for microarray-based genome-composition analysis (2002)
Kim, Charles C, Joyce, Elizabeth A, Chan, Kaman, Falkow, Stanley
Abstract Background Whereas genome sequencing has given us high-resolution pictures of many different species of bacteria, microarrays provide a means of obtaining information on genome composition...
Typhimurium Dna Microarray, Kaman Chan, Stephen Baker, Charles C. Kim, Corrella S. Detweiler, Gordon Dougan, ...
The genus Salmonella consists of over 2,200 serovars that differ in their host range and ability to cause disease despite their close genetic relatedness. The genetic factors that influence each...
Infectious Multiple Drug Resistance in the Enterobacteriaceae. (1998)
A gene encoding a heat-stable enterotoxin (ST) from an Escherichia coli strain isolated from a human with diarrhea has been cloned and characterized by nucleotide sequence analysis. The gene was...
Infectious Multiple Drug Resistance in the Enterobacteriaceae. (1998)
The structural gene for plasmid-mediated ampicillin resistance resides upon a 3.2 x 10 to the 6th power dalton transposable sequence, TnA, flanked by short inverted repeated sequences which accompany...
Infectious Multiple Drug Resistance in the Enterobacteriaceae. (1998)
Tobrymycin-resistant burn wound isolates from five different bacterial genera contained a common 68 x 10 to the 6th power dalton R plasmid that could be transmitted by conjugation to Escherichia coli...
Infectious Multiple Drug Resistance in the Enterobacteriaceae. (1998)
Genetic and Molecular studies of R-plasmids have shown that their genome is clearly divisible into three distinct regions. The tra region concerned with conjugation functions is conserved to a high...
Moseley, Steve L., Hardy, Jonathan W., Hug, M. Imdadul, Echeverria, Peter, Falkow, Stanley
A gene encoding a heat-stable enterotoxin (ST) from an Escherichia coli strain isolated from a human with diarrhea was cloned and characterized by nucleotide sequence analysis. The gene was found to...
A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains
Salama, Nina, Guillemin, Karen, McDaniel, Timothy K., Sherlock, Gavin, Tompkins, Lucy, Falkow, Stanley
Helicobacter pylori colonizes the stomach of half of the world's population, causing a wide spectrum of disease ranging from asymptomatic gastritis to ulcers to gastric cancer. Although the basis for...
Sequence anomalies in the Cag7 gene of the Helicobacter pylori pathogenicity island
Liu, Guoying, McDaniel, Timothy K., Falkow, Stanley, Karlin, Samuel
The severity of Helicobacter pylori-related disease is correlated with a pathogenicity island (the Cag region of about 26 genes) whose presence is associated with the up-regulation of an IL-8...
Edelstein, Paul H., Edelstein, Martha A. C., Higa, Futoshi, Falkow, Stanley
Legionella pneumophila is the cause of Legionnaires’ disease, which is a form of potentially fatal pneumonia. To identify genes required for virulence of the bacterium, a library of 1,386 L....
Yersinia signals macrophages to undergo apoptosis and YopJ is necessary for this cell death
Monack, Denise M., Mecsas, Joan, Ghori, Nafisa, Falkow, Stanley
Pathogenic Yersinia spp. carry a large common plasmid that encodes a number of essential virulence determinants. Included in these factors are the Yersinia-secreted proteins called Yops. We analyzed...
The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1
Hersh, David, Monack, Denise M., Smith, Mark R., Ghori, Nafisa, Falkow, Stanley, Zychlinsky, Arturo
Recently, Salmonella spp. were shown to induce apoptosis in infected macrophages. The mechanism responsible for this process is unknown. In this report, we establish that the Inv-Spa type III...
Detweiler, Corrella S., Cunanan, Dolores B., Falkow, Stanley
Bacterial pathogens manipulate host cells to promote pathogen survival and dissemination. We used a 22,571 human cDNA microarray to identify host pathways that are affected by the Salmonella enterica...
Helicobacter pylori genetic diversity within the gastric niche of a single human host
Israel, Dawn A., Salama, Nina, Krishna, Uma, Rieger, Ulrich M., Atherton, John C., Falkow, Stanley, ...
Isolates of the gastric pathogen Helicobacter pylori harvested from different individuals are highly polymorphic. Strain variation also has been observed within a single host. To more fully ascertain...
OmpR Regulates the Two-Component System SsrA-SsrB in Salmonella Pathogenicity Island 2
Lee, Anthea K., Detweiler, Corrella S., Falkow, Stanley
Salmonella pathogenicity island 2 (SPI-2) encodes a putative, two-component regulatory system, SsrA-SsrB, which regulates a type III secretion system needed for replication inside macrophages and...
Cirillo, Jeffrey D., Cirillo, Suat L. G., Yan, Ling, Bermudez, Luiz E., Falkow, Stanley, Tompkins, Lucy S.
Since Legionella pneumophila is an intracellular pathogen, entry into and replication within host cells are thought to be critical to its ability to cause disease. L. pneumophila grown in one of its...
Valdivia, Raphael H., Cirillo, Daniela M., Lee, Anthea K., Bouley, Donna M., Falkow, Stanley
We have characterized a host-induced virulence gene, mig-14, that is required for fatal infection in the mouse model of enteric fever. mig-14 is present in all Salmonella enterica subspecies I...
Salama, Nina R., Otto, Glen, Tompkins, Lucy, Falkow, Stanley
Helicobacter pylori, the causative agent of gastritis and ulcer disease in humans, secretes a toxin called VacA (vacuolating cytotoxin) into culture supernatants. VacA was initially characterized and...
Polesky, Andrea H., Ross, Julianna T. D., Falkow, Stanley, Tompkins, Lucy S.
Legionella pneumophila is a facultative intracellular gram-negative rod that causes pneumonia in humans. Free-living amoebas are thought to serve as a reservoir for Legionella infections....
Mecsas, Joan, Bilis, Inna, Falkow, Stanley
Yersinia pseudotuberculosis localizes to the distal ileum, cecum, and proximal colon of the gastrointestinal tract after oral infection. Using signature-tagged mutagenesis, we isolated 13 Y....
Mimicry of a G Protein Mutation by Pertussis Toxin Expression in Transgenic Caenorhabditis elegans
Pathogens produce virulence factors that interact directly with host molecules, but in many cases the host targets are unknown. The genetic and molecular identification of these orphan targets is...
Dynamic Nature of Host-Pathogen Interactions in Mycobacterium marinum Granulomas
Bouley, Donna M., Ghori, Nafisa, Mercer, K. Lynne, Falkow, Stanley, Ramakrishnan, Lalita
Mycobacterium marinum causes long-term subclinical granulomatous infection in immunocompetent leopard frogs (Rana pipiens). These granulomas, organized collections of activated macrophages, share...
Constitutive and Inducible Green Fluorescent Protein Expression in Bartonella henselae
Lee, Anthea K., Falkow, Stanley
The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing...
Identification of Streptococcus bovis Biotype I Strains among S. bovis Clinical Isolates by PCR
Songy, Wanda B., Ruoff, Kathryn L., Facklam, Richard R., Ferraro, Mary J., Falkow, Stanley
Streptococcus bovis causes 24% of all streptococcal infective endocarditis cases. There are many reports linking both S. bovis bacteremia and endocarditis with various forms of gastrointestinal...
Complex pattern of Mycobacterium marinum gene expression during long-term granulomatous infection
Chan, Kaman, Knaak, Timothy, Satkamp, Laura, Humbert, Olivier, Falkow, Stanley, Ramakrishnan, Lalita
During latent infection of humans with Mycobacterium tuberculosis, bacteria persist in the asymptomatic host within granulomas, organized collections of differentiated macrophages, and other immune...
Improved analytical methods for microarray-based genome-composition analysis
Kim, Charles C, Joyce, Elizabeth A, Chan, Kaman, Falkow, Stanley
Genome-composition analysis using microarrays can be used to categorize genes into 'present' and 'divergent' categories. This involves selecting a signal value that is used as a cutoff to...
mig-14 Is a Salmonella Gene That Plays a Role in Bacterial Resistance to Antimicrobial Peptides
Brodsky, Igor E., Ernst, Robert K., Miller, Samuel I., Falkow, Stanley
It was previously demonstrated that the mig-14 gene of Salmonella enterica serovar Typhimurium is necessary for bacterial proliferation in the liver and spleen of mice following intragastric...
Guillemin, Karen, Salama, Nina R., Tompkins, Lucy S., Falkow, Stanley
Helicobacter pylori infects over half the world's population and causes a wide range of diseases, including gastritis, peptic ulcer, and two forms of gastric cancer. H. pylori infection elicits a...
Chan, Kaman, Baker, Stephen, Kim, Charles C., Detweiler, Corrella S., Dougan, Gordon, Falkow, Stanley
The genus Salmonella consists of over 2,200 serovars that differ in their host range and ability to cause disease despite their close genetic relatedness. The genetic factors that influence each...
Thompson, Lucinda J., Merrell, D. Scott, Neilan, Brett A., Mitchell, Hazel, Lee, Adrian, Falkow, Stanley
The global pattern of growth-phase-dependent gene expression of Helicobacter pylori during in vitro culture was analyzed by using a high-density DNA microarray. To detect consistent coordinated gene...
Kim, Charles C., Monack, Denise, Falkow, Stanley
Two acidified nitrite-inducible genes of Salmonella enterica serovar Typhimurium were identified with a green fluorescent protein-based promoter-trap screen. The nitrite-inducible promoters were...
pH-Regulated Gene Expression of the Gastric Pathogen Helicobacter pylori
Merrell, D. Scott, Goodrich, Maria L., Otto, Glen, Tompkins, Lucy S., Falkow, Stanley
Colonization by the gastric pathogen Helicobacter pylori has been shown to be intricately linked to the development of gastritis, ulcers, and gastric malignancy. Little is known about mechanisms...
Israel, Dawn A., Salama, Nina, Arnold, Carrie N., Moss, Steven F., Ando, Takafumi, Wirth, Hans-Peter, ...
Helicobacter pylori enhances the risk for ulcer disease and gastric cancer, yet only a minority of H. pylori–colonized individuals develop disease. We examined the ability of two H. pylori isolates...
Protective immunity against Helicobacter is characterized by a unique transcriptional signature
Mueller, Anne, O'Rourke, Jani, Chu, Pauline, Kim, Charles C., Sutton, Philip, Lee, Adrian, ...
Immunization with a whole-cell sonicate vaccine of Helicobacter felis in conjunction with cholera toxin as a mucosal adjuvant induces long-term protective immunity in a majority of laboratory mice....
Growth Phase-Dependent Response of Helicobacter pylori to Iron Starvation
Merrell, D. Scott, Thompson, Lucinda J., Kim, Charles C., Mitchell, Hazel, Tompkins, Lucy S., Lee, Adrian, ...
Iron is an essential nutrient that is often found in extremely limited available quantities within eukaryotic hosts. Because of this, many pathogenic bacteria have developed regulated networks of...
R62, a Naturally Occurring Hybrid R Plasmid
Guerry, Patricia, Falkow, Stanley, Datta, Naomi
R62, a naturally occurring R factor, was shown to be a single deoxyribonucleic acid molecule composed of polynucleotide sequences typical of I group plasmids and also sequences typical of the N...
Crosa, Jorge H., Brenner, Don J., Falkow, Stanley
Bacterial and plasmid homo- and heteroduplexes have been analyzed with a single-strand specific endonuclease, S1, of Aspergillus oryzae. Under appropriate assay conditions, there was a high degree of...
Hershfield, Vickers, LeBlanc, Donald J., Falkow, Stanley
Superinfection of an Escherichia coli R+ strain with a closely related R-factor was associated with the accumulation of replicative intermediates of the superinfecting deoxyribonucleic acid. This...
Polynucleotide Sequence Relationships Among Some Bacterial Plasmids
Guerry, Patricia, Falkow, Stanley
The deoxyribonucleic acid of F-like plasmids appear to share a high degree of nucleotide similarity with each other but are not highly related to I-like plasmids.
Polynucleotide Sequence Divergence Among Strains of Escherichia coli and Closely Related Organisms
Brenner, Don J., Fanning, G. R., Skerman, F. J., Falkow, Stanley
Polynucleotide sequence similarity tests were carried out to determine the extent of divergence present in a number of Escherichia coli strains, obtained from diverse human, animal, and laboratory...
Silver, Richard P., Falkow, Stanley
The molecular nature of R-factor deoxyribonucleic acid (DNA) was examined in Escherichia coli by using a method for the specific labeling of the derepressed R factor, R1, in a female cell after...
Studies on Resistance Transfer Factor Deoxyribonucleic Acid in Escherichia coli
Silver, Richard P., Falkow, Stanley
A variant of the derepressed R factor, R1, which does not contain any of the drug resistance markers, and represents, in large part, the resistance transfer factor (RTF) was studied in Escherichia...
Physical Studies of the Drug-Resistance Transfer Factor in Proteus
Haapala, Daniel K., Falkow, Stanley
The transfer unit, RTF, derived from an R factor has been transferred to Proteus mirabilis and characterized physiochemically.
Intramolecular Heterogeneity of the Deoxyribonucleic Acid of Temperate Bacteriophages
Falkow, Stanley, Cowie, Dean B.
Spectral changes accompanying the thermal denaturation of phage deoxyribonucleic acid suggested that λ is not unique in possessing large-scale intramolecular heterogeneity and nucleotide clustering;...
RECIPIENT ABILITY OF SALMONELLA TYPHOSA IN GENETIC CROSSES WITH ESCHERICHIA COLI1
Johnson, E. M., Falkow, Stanley, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Stanley Falkow, and L. S. Baron. Recipient ability of Salmonella typhosa in genetic crosses with Escherichia coli. J....
TRANSFER OF EPISOMIC ELEMENTS TO PROTEUS I. : Transfer of F-Linked Chromosomal Determinants
Falkow, Stanley, Wohlhieter, J. A., Citarella, R. V., Baron, L. S.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), J. A. Wohlhieter, R. V. Citarella, and L. S. Baron. Transfer of episomic elements to Proteus. I. Transfer of F-linked...
CHROMOSOME TRANSFER KINETICS OF SALMONELLAHfr STRAINS
Johnson, E. M., Falkow, Stanley, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Stanley Falkow, and L. S. Baron. Chromosome transfer kinetics of Salmonella Hfr strains. J. Bacteriol. 88:395–400....
CHARACTERIZATION OF AN HFR STRAIN OF SHIGELLA FLEXNERI1
Schneider, Herman, Falkow, Stanley
Schneider, Herman (Walter Reed Army Institute of Research, Washington, D.C.), and Stanley Falkow. Characterization of an Hfr strain of Shigella flexneri. J. Bacteriol. 88:682–689. 1964.—A Hfr...
Falkow, Stanley, Wohlhieter, J. A., Citarella, R. V., Baron, L. S.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), J. A. Wohlhieter, R. V. Citarella, and L. S. Baron. Transfer of episomic elements to Proteus. II. Nature of lac+Proteus...
Restoration of Virulence to a Strain of Shigella flexneri by Mating with Escherichia coli
Formal, Samuel B., LaBrec, E. H., Schneider, H., Falkow, Stanley
Formal, Samuel B. (Walter Reed Army Institute of Research, Washington, D.C.), E. H. LaBrec, H. Schneider, and Stanley Falkow. Restoration of virulence to a strain of Shigella flexneri by mating with...
EPISOMIC ELEMENT IN A STRAIN OF SALMONELLA TYPHOSA
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.) and L. S. Baron. Episomic element in a strain of Salmonella typhosa. J. Bacteriol. 84:581–589. 1962.—An episomic...
GENETIC HOMOLOGY BETWEEN ESCHERICHIA COLI K-12 AND SALMONELLA
Falkow, Stanley, Rownd, R., Baron, L. S.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), Robert Rownd, and L. S. Baron. Genetic homology between Escherichia coli K-12 and Salmonella. J. Bacteriol. 84:1303–1312....
DEOXYRIBONUCLEIC ACID BASE COMPOSITION OF PROTEUS AND PROVIDENCE ORGANISMS
Falkow, Stanley, Ryman, I. R., Washington, O.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington D.C.), I. R. Ryman, and O. Washington. Deoxyribonucleic acid base composition of Proteus and Providence organisms. J. Bacteriol....
VIRULENCE OF ESCHERICHIA-SHIGELLA GENETIC HYBRIDS FOR THE GUINEA PIG
Falkow, Stanley, Schneider, H., Baron, L. S., Formal, Samuel B.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), H. Schneider, L. S. Baron, and S. B. Formal. Virulence of Escherichia-Shigella hybrids for the guinea pig. J. Bacteriol....
Polynucleotide Sequence Relationships among Members of Enterobacteriaceae
Brenner, Don J., Fanning, George R., Johnson, Karl E., Citarella, R. V., Falkow, Stanley
Polynucleotide relationships were examined among many representatives of the Enterobacteriaceae by means of agar, membrane filter, and hydroxyapatite procedures. The amount of deoxyribonucleic acid...
Behavior of Coliphage Lambda in Hybrids Between Escherichia coli and Salmonella
Baron, L. S., Penido, Elisa, Ryman, I. R., Falkow, Stanley
Salmonella typhosa hybrids able to adsorb lambda were obtained by mating S. typhosa recipients with Escherichia coli K-12 donors. After adsorption of wild-type λ to these S. typhosa hybrids, no...
Defective phage λdg, when present in certain Salmonella typhosa hybrids, could be eliminated with acridine orange or ethidium bromide treatment. The λdg deoxyribonucleic acid could be separated...
General Method for the Isolation of Plasmid Deoxyribonucleic Acid
Guerry, Patricia, LeBlanc, D. J., Falkow, Stanley
Plasmid deoxyribonucleic acid (DNA) ranging from 5 × 106 to 65 × 106 daltons may be isolated from chromosomal DNA by the preferential precipitation of the higher-molecular-weight chromosomal DNA in...
Molecular Nature of Two Nonconjugative Plasmids Carrying Drug Resistance Genes
Guerry, Patricia, Van Embden, Jan, Falkow, Stanley
Two nonconjugative R-plasmids, N-SuSm and N-Tc, have been characterized. Both were of relatively small size (5 × 106 to 6 × 106 daltons) and present in multiple copies within their respective...
Mueller, Anne, O'Rourke, Jani, Grimm, Jan, Guillemin, Karen, Dixon, Michael F., Lee, Adrian, ...
Long-term colonization of humans with Helicobacter pylori can cause the development of gastric B cell mucosa-associated lymphoid tissue lymphoma, yet little is known about the sequence of molecular...
Gaynor, Erin C., Cawthraw, Shaun, Manning, Georgina, MacKichan, Joanna K., Falkow, Stanley, Newell, Diane G.
The genome sequence of the enteric bacterial pathogen Campylobacter jejuni NCTC 11168 (11168-GS) was published in 2000, providing a valuable resource for the identification of C. jejuni-specific...
Restriction in Genetic Crosses Between Escherichia coli and Shigella flexneri
Falkow, Stanley, Formal, S. B.
Shigella flexneri restricts Escherichia coli deoxyribonucleic acid (DNA) and can modify phage DNA so that it is restricted in E. coli.
Protection of Monkeys Against Experimental Shigellosis with Attenuated Vaccines
Formal, Samuel B., LaBrec, E. H., Palmer, Amos, Falkow, Stanley
Formal, Samuel B. (Walter Reed Army Institute of Research, Washington, D.C.), E. H. LaBrec, Amos Palmer, and Stanley Falkow. Protection of monkeys against experimental shigellosis with attenuated...
Nucleic Acid Homologies of Selected Bacteria, L Forms, and Mycoplasma Species1
Rogul, M., McGee, Z. A., Wittler, R. G., Falkow, Stanley
Rogul, M. (Walter Reed Army Institute of Research, Washington, D.C.), Z. A. McGee, R. G. Wittler, and Stanley Falkow. Nucleic acid homologies of selected bacteria, L forms, and Mycoplasma species. J....
Leonard, Edward E., Tompkins, Lucy S., Falkow, Stanley, Nachamkin, Irving
We asked whether Campylobacter jejuni isolated from patients with Guillain-Barré syndrome (GBS) differ from isolates isolated from patients with uncomplicated gastrointestinal infection using DNA...
Plasmid-Mediated Beta-Lactamase Production in Neisseria gonorrhoeae
Elwell, Lynn P., Roberts, Marilyn, Mayer, Leonard W., Falkow, Stanley
Several β-lactamase-producing, penicillin-resistant strains of Neisseria gonorrhoeae were examined for R plasmids. Penicillin-resistant strains isolated from men returning from the Far East and...
Joyce, Elizabeth A., Kawale, Amita, Censini, Stefano, Kim, Charles C., Covacci, Antonello, Falkow, Stanley
Streptococcus pneumoniae causes several diseases, including otitis media, pneumonia, and meningitis. Although little is known about the regulation of or how individual pneumococcal factors contribute...
Relationship Between Colicin V Activity and Virulence in Escherichia coli
Quackenbush, Robert L., Falkow, Stanley
Colicin V activity is not essential to ColV plasmid-mediated virulence enhancement in Escherichia coli.
In Vivo Conjugal Transfer of R Plasmids in Neisseria gonorrhoeae
Roberts, Marilyn, Falkow, Stanley
In vivo R plasmid transfer between two Neisseria gonorrhoeae strains was detected in the absence of antibiotic pressure.
Plasmid-Associated Enterotoxin Production in a Strain of Escherichia coli Isolated from Humans
Skerman, F. J., Formal, S. B., Falkow, Stanley
A strain of Escherichia coli isolated from an epidemic of “nonspecific” diarrhea has been shown to harbor a plasmid which specifies enterotoxin production.
Characterization of Plasmid Deoxyribonucleic Acid from Neisseria gonorrhoeae
Mayer, Leonard W., Holmes, King K., Falkow, Stanley
Five of six independent gonococcal isolates have been found to harbor a plasmid pool of covalently closed-circular deoxyribonucleic acid about 2.4 × 106 daltons in size. The plasmid species were...
Kim, Charles C., Falkow, Stanley
Survival and replication in the intracellular environment are critical components of the ability of Salmonella enterica serovar Typhimurium to establish systemic infection in the murine host....
Thompson, Lucinda J., Danon, Stephen J., Wilson, John E., O'Rourke, Jani L., Salama, Nina R., Falkow, Stanley, ...
The mouse model of Helicobacter pylori-induced disease using Sydney strain 1 (SS1) has been used extensively in Helicobacter research. Herein we describe the isolation and characterization of a new...
Global Transposon Mutagenesis and Essential Gene Analysis of Helicobacter pylori†
Salama, Nina R., Shepherd, Benjamin, Falkow, Stanley
We have constructed a genome-saturating mutant library of the human gastric pathogen Helicobacter pylori. Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363...
Gaynor, Erin C., Cawthraw, Shaun, Manning, Georgina, MacKichan, Joanna K., Falkow, Stanley, Newell, Diane G.
Chan, Kaman, Kim, Charles C., Falkow, Stanley
DNA microarrays provide an opportunity to combine the principles of signature-tagged mutagenesis (STM) with microarray technology to identify potentially important bacterial virulence genes. The...
Episomic Transfer between Salmonella Typhosa and Serratia Marcescens
Falkow, Stanley, Marmur, J., Carey, W. F., Spilman, W. M., Baron, L. S.
A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains
Salama, Nina, Guillemin, Karen, McDaniel, Timothy K., Sherlock, Gavin, Tompkins, Lucy, Falkow, Stanley
Helicobacter pylori colonizes the stomach of half of the world's population, causing a wide spectrum of disease ranging from asymptomatic gastritis to ulcers to gastric cancer. Although the basis for...
Sequence anomalies in the Cag7 gene of the Helicobacter pylori pathogenicity island
Liu, Guoying, McDaniel, Timothy K., Falkow, Stanley, Karlin, Samuel
The severity of Helicobacter pylori-related disease is correlated with a pathogenicity island (the Cag region of about 26 genes) whose presence is associated with the up-regulation of an IL-8...
Edelstein, Paul H., Edelstein, Martha A. C., Higa, Futoshi, Falkow, Stanley
Legionella pneumophila is the cause of Legionnaires’ disease, which is a form of potentially fatal pneumonia. To identify genes required for virulence of the bacterium, a library of 1,386 L....
Yersinia signals macrophages to undergo apoptosis and YopJ is necessary for this cell death
Monack, Denise M., Mecsas, Joan, Ghori, Nafisa, Falkow, Stanley
Pathogenic Yersinia spp. carry a large common plasmid that encodes a number of essential virulence determinants. Included in these factors are the Yersinia-secreted proteins called Yops. We analyzed...
The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1
Hersh, David, Monack, Denise M., Smith, Mark R., Ghori, Nafisa, Falkow, Stanley, Zychlinsky, Arturo
Recently, Salmonella spp. were shown to induce apoptosis in infected macrophages. The mechanism responsible for this process is unknown. In this report, we establish that the Inv-Spa type III...
Detweiler, Corrella S., Cunanan, Dolores B., Falkow, Stanley
Bacterial pathogens manipulate host cells to promote pathogen survival and dissemination. We used a 22,571 human cDNA microarray to identify host pathways that are affected by the Salmonella enterica...
Helicobacter pylori genetic diversity within the gastric niche of a single human host
Israel, Dawn A., Salama, Nina, Krishna, Uma, Rieger, Ulrich M., Atherton, John C., Falkow, Stanley, ...
Isolates of the gastric pathogen Helicobacter pylori harvested from different individuals are highly polymorphic. Strain variation also has been observed within a single host. To more fully ascertain...
OmpR Regulates the Two-Component System SsrA-SsrB in Salmonella Pathogenicity Island 2
Lee, Anthea K., Detweiler, Corrella S., Falkow, Stanley
Salmonella pathogenicity island 2 (SPI-2) encodes a putative, two-component regulatory system, SsrA-SsrB, which regulates a type III secretion system needed for replication inside macrophages and...
Cirillo, Jeffrey D., Cirillo, Suat L. G., Yan, Ling, Bermudez, Luiz E., Falkow, Stanley, Tompkins, Lucy S.
Since Legionella pneumophila is an intracellular pathogen, entry into and replication within host cells are thought to be critical to its ability to cause disease. L. pneumophila grown in one of its...
Valdivia, Raphael H., Cirillo, Daniela M., Lee, Anthea K., Bouley, Donna M., Falkow, Stanley
We have characterized a host-induced virulence gene, mig-14, that is required for fatal infection in the mouse model of enteric fever. mig-14 is present in all Salmonella enterica subspecies I...
Salama, Nina R., Otto, Glen, Tompkins, Lucy, Falkow, Stanley
Helicobacter pylori, the causative agent of gastritis and ulcer disease in humans, secretes a toxin called VacA (vacuolating cytotoxin) into culture supernatants. VacA was initially characterized and...
Polesky, Andrea H., Ross, Julianna T. D., Falkow, Stanley, Tompkins, Lucy S.
Legionella pneumophila is a facultative intracellular gram-negative rod that causes pneumonia in humans. Free-living amoebas are thought to serve as a reservoir for Legionella infections....
Mecsas, Joan, Bilis, Inna, Falkow, Stanley
Yersinia pseudotuberculosis localizes to the distal ileum, cecum, and proximal colon of the gastrointestinal tract after oral infection. Using signature-tagged mutagenesis, we isolated 13 Y....
Mimicry of a G Protein Mutation by Pertussis Toxin Expression in Transgenic Caenorhabditis elegans
Pathogens produce virulence factors that interact directly with host molecules, but in many cases the host targets are unknown. The genetic and molecular identification of these orphan targets is...
Dynamic Nature of Host-Pathogen Interactions in Mycobacterium marinum Granulomas
Bouley, Donna M., Ghori, Nafisa, Mercer, K. Lynne, Falkow, Stanley, Ramakrishnan, Lalita
Mycobacterium marinum causes long-term subclinical granulomatous infection in immunocompetent leopard frogs (Rana pipiens). These granulomas, organized collections of activated macrophages, share...
Constitutive and Inducible Green Fluorescent Protein Expression in Bartonella henselae
Lee, Anthea K., Falkow, Stanley
The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing...
Identification of Streptococcus bovis Biotype I Strains among S. bovis Clinical Isolates by PCR
Songy, Wanda B., Ruoff, Kathryn L., Facklam, Richard R., Ferraro, Mary J., Falkow, Stanley
Streptococcus bovis causes 24% of all streptococcal infective endocarditis cases. There are many reports linking both S. bovis bacteremia and endocarditis with various forms of gastrointestinal...
Complex pattern of Mycobacterium marinum gene expression during long-term granulomatous infection
Chan, Kaman, Knaak, Timothy, Satkamp, Laura, Humbert, Olivier, Falkow, Stanley, Ramakrishnan, Lalita
During latent infection of humans with Mycobacterium tuberculosis, bacteria persist in the asymptomatic host within granulomas, organized collections of differentiated macrophages, and other immune...
Improved analytical methods for microarray-based genome-composition analysis
Kim, Charles C, Joyce, Elizabeth A, Chan, Kaman, Falkow, Stanley
Genome-composition analysis using microarrays can be used to categorize genes into 'present' and 'divergent' categories. This involves selecting a signal value that is used as a cutoff to...
mig-14 Is a Salmonella Gene That Plays a Role in Bacterial Resistance to Antimicrobial Peptides
Brodsky, Igor E., Ernst, Robert K., Miller, Samuel I., Falkow, Stanley
It was previously demonstrated that the mig-14 gene of Salmonella enterica serovar Typhimurium is necessary for bacterial proliferation in the liver and spleen of mice following intragastric...
Guillemin, Karen, Salama, Nina R., Tompkins, Lucy S., Falkow, Stanley
Helicobacter pylori infects over half the world's population and causes a wide range of diseases, including gastritis, peptic ulcer, and two forms of gastric cancer. H. pylori infection elicits a...
Chan, Kaman, Baker, Stephen, Kim, Charles C., Detweiler, Corrella S., Dougan, Gordon, Falkow, Stanley
The genus Salmonella consists of over 2,200 serovars that differ in their host range and ability to cause disease despite their close genetic relatedness. The genetic factors that influence each...
Thompson, Lucinda J., Merrell, D. Scott, Neilan, Brett A., Mitchell, Hazel, Lee, Adrian, Falkow, Stanley
The global pattern of growth-phase-dependent gene expression of Helicobacter pylori during in vitro culture was analyzed by using a high-density DNA microarray. To detect consistent coordinated gene...
Kim, Charles C., Monack, Denise, Falkow, Stanley
Two acidified nitrite-inducible genes of Salmonella enterica serovar Typhimurium were identified with a green fluorescent protein-based promoter-trap screen. The nitrite-inducible promoters were...
pH-Regulated Gene Expression of the Gastric Pathogen Helicobacter pylori
Merrell, D. Scott, Goodrich, Maria L., Otto, Glen, Tompkins, Lucy S., Falkow, Stanley
Colonization by the gastric pathogen Helicobacter pylori has been shown to be intricately linked to the development of gastritis, ulcers, and gastric malignancy. Little is known about mechanisms...
Israel, Dawn A., Salama, Nina, Arnold, Carrie N., Moss, Steven F., Ando, Takafumi, Wirth, Hans-Peter, ...
Helicobacter pylori enhances the risk for ulcer disease and gastric cancer, yet only a minority of H. pylori–colonized individuals develop disease. We examined the ability of two H. pylori isolates...
Protective immunity against Helicobacter is characterized by a unique transcriptional signature
Mueller, Anne, O'Rourke, Jani, Chu, Pauline, Kim, Charles C., Sutton, Philip, Lee, Adrian, ...
Immunization with a whole-cell sonicate vaccine of Helicobacter felis in conjunction with cholera toxin as a mucosal adjuvant induces long-term protective immunity in a majority of laboratory mice....
Growth Phase-Dependent Response of Helicobacter pylori to Iron Starvation
Merrell, D. Scott, Thompson, Lucinda J., Kim, Charles C., Mitchell, Hazel, Tompkins, Lucy S., Lee, Adrian, ...
Iron is an essential nutrient that is often found in extremely limited available quantities within eukaryotic hosts. Because of this, many pathogenic bacteria have developed regulated networks of...
R62, a Naturally Occurring Hybrid R Plasmid
Guerry, Patricia, Falkow, Stanley, Datta, Naomi
R62, a naturally occurring R factor, was shown to be a single deoxyribonucleic acid molecule composed of polynucleotide sequences typical of I group plasmids and also sequences typical of the N...
Crosa, Jorge H., Brenner, Don J., Falkow, Stanley
Bacterial and plasmid homo- and heteroduplexes have been analyzed with a single-strand specific endonuclease, S1, of Aspergillus oryzae. Under appropriate assay conditions, there was a high degree of...
Hershfield, Vickers, LeBlanc, Donald J., Falkow, Stanley
Superinfection of an Escherichia coli R+ strain with a closely related R-factor was associated with the accumulation of replicative intermediates of the superinfecting deoxyribonucleic acid. This...
Polynucleotide Sequence Relationships Among Some Bacterial Plasmids
Guerry, Patricia, Falkow, Stanley
The deoxyribonucleic acid of F-like plasmids appear to share a high degree of nucleotide similarity with each other but are not highly related to I-like plasmids.
Polynucleotide Sequence Divergence Among Strains of Escherichia coli and Closely Related Organisms
Brenner, Don J., Fanning, G. R., Skerman, F. J., Falkow, Stanley
Polynucleotide sequence similarity tests were carried out to determine the extent of divergence present in a number of Escherichia coli strains, obtained from diverse human, animal, and laboratory...
Silver, Richard P., Falkow, Stanley
The molecular nature of R-factor deoxyribonucleic acid (DNA) was examined in Escherichia coli by using a method for the specific labeling of the derepressed R factor, R1, in a female cell after...
Studies on Resistance Transfer Factor Deoxyribonucleic Acid in Escherichia coli
Silver, Richard P., Falkow, Stanley
A variant of the derepressed R factor, R1, which does not contain any of the drug resistance markers, and represents, in large part, the resistance transfer factor (RTF) was studied in Escherichia...
Physical Studies of the Drug-Resistance Transfer Factor in Proteus
Haapala, Daniel K., Falkow, Stanley
The transfer unit, RTF, derived from an R factor has been transferred to Proteus mirabilis and characterized physiochemically.
Intramolecular Heterogeneity of the Deoxyribonucleic Acid of Temperate Bacteriophages
Falkow, Stanley, Cowie, Dean B.
Spectral changes accompanying the thermal denaturation of phage deoxyribonucleic acid suggested that λ is not unique in possessing large-scale intramolecular heterogeneity and nucleotide clustering;...
RECIPIENT ABILITY OF SALMONELLA TYPHOSA IN GENETIC CROSSES WITH ESCHERICHIA COLI1
Johnson, E. M., Falkow, Stanley, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Stanley Falkow, and L. S. Baron. Recipient ability of Salmonella typhosa in genetic crosses with Escherichia coli. J....
TRANSFER OF EPISOMIC ELEMENTS TO PROTEUS I. : Transfer of F-Linked Chromosomal Determinants
Falkow, Stanley, Wohlhieter, J. A., Citarella, R. V., Baron, L. S.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), J. A. Wohlhieter, R. V. Citarella, and L. S. Baron. Transfer of episomic elements to Proteus. I. Transfer of F-linked...
CHROMOSOME TRANSFER KINETICS OF SALMONELLAHfr STRAINS
Johnson, E. M., Falkow, Stanley, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Stanley Falkow, and L. S. Baron. Chromosome transfer kinetics of Salmonella Hfr strains. J. Bacteriol. 88:395–400....
CHARACTERIZATION OF AN HFR STRAIN OF SHIGELLA FLEXNERI1
Schneider, Herman, Falkow, Stanley
Schneider, Herman (Walter Reed Army Institute of Research, Washington, D.C.), and Stanley Falkow. Characterization of an Hfr strain of Shigella flexneri. J. Bacteriol. 88:682–689. 1964.—A Hfr...
Falkow, Stanley, Wohlhieter, J. A., Citarella, R. V., Baron, L. S.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), J. A. Wohlhieter, R. V. Citarella, and L. S. Baron. Transfer of episomic elements to Proteus. II. Nature of lac+Proteus...
Restoration of Virulence to a Strain of Shigella flexneri by Mating with Escherichia coli
Formal, Samuel B., LaBrec, E. H., Schneider, H., Falkow, Stanley
Formal, Samuel B. (Walter Reed Army Institute of Research, Washington, D.C.), E. H. LaBrec, H. Schneider, and Stanley Falkow. Restoration of virulence to a strain of Shigella flexneri by mating with...
EPISOMIC ELEMENT IN A STRAIN OF SALMONELLA TYPHOSA
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.) and L. S. Baron. Episomic element in a strain of Salmonella typhosa. J. Bacteriol. 84:581–589. 1962.—An episomic...
GENETIC HOMOLOGY BETWEEN ESCHERICHIA COLI K-12 AND SALMONELLA
Falkow, Stanley, Rownd, R., Baron, L. S.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), Robert Rownd, and L. S. Baron. Genetic homology between Escherichia coli K-12 and Salmonella. J. Bacteriol. 84:1303–1312....
DEOXYRIBONUCLEIC ACID BASE COMPOSITION OF PROTEUS AND PROVIDENCE ORGANISMS
Falkow, Stanley, Ryman, I. R., Washington, O.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington D.C.), I. R. Ryman, and O. Washington. Deoxyribonucleic acid base composition of Proteus and Providence organisms. J. Bacteriol....
VIRULENCE OF ESCHERICHIA-SHIGELLA GENETIC HYBRIDS FOR THE GUINEA PIG
Falkow, Stanley, Schneider, H., Baron, L. S., Formal, Samuel B.
Falkow, Stanley (Walter Reed Army Institute of Research, Washington, D.C.), H. Schneider, L. S. Baron, and S. B. Formal. Virulence of Escherichia-Shigella hybrids for the guinea pig. J. Bacteriol....
Polynucleotide Sequence Relationships among Members of Enterobacteriaceae
Brenner, Don J., Fanning, George R., Johnson, Karl E., Citarella, R. V., Falkow, Stanley
Polynucleotide relationships were examined among many representatives of the Enterobacteriaceae by means of agar, membrane filter, and hydroxyapatite procedures. The amount of deoxyribonucleic acid...
Behavior of Coliphage Lambda in Hybrids Between Escherichia coli and Salmonella
Baron, L. S., Penido, Elisa, Ryman, I. R., Falkow, Stanley
Salmonella typhosa hybrids able to adsorb lambda were obtained by mating S. typhosa recipients with Escherichia coli K-12 donors. After adsorption of wild-type λ to these S. typhosa hybrids, no...
Defective phage λdg, when present in certain Salmonella typhosa hybrids, could be eliminated with acridine orange or ethidium bromide treatment. The λdg deoxyribonucleic acid could be separated...
General Method for the Isolation of Plasmid Deoxyribonucleic Acid
Guerry, Patricia, LeBlanc, D. J., Falkow, Stanley
Plasmid deoxyribonucleic acid (DNA) ranging from 5 × 106 to 65 × 106 daltons may be isolated from chromosomal DNA by the preferential precipitation of the higher-molecular-weight chromosomal DNA in...
Molecular Nature of Two Nonconjugative Plasmids Carrying Drug Resistance Genes
Guerry, Patricia, Van Embden, Jan, Falkow, Stanley
Two nonconjugative R-plasmids, N-SuSm and N-Tc, have been characterized. Both were of relatively small size (5 × 106 to 6 × 106 daltons) and present in multiple copies within their respective...
Mueller, Anne, O'Rourke, Jani, Grimm, Jan, Guillemin, Karen, Dixon, Michael F., Lee, Adrian, ...
Long-term colonization of humans with Helicobacter pylori can cause the development of gastric B cell mucosa-associated lymphoid tissue lymphoma, yet little is known about the sequence of molecular...
Gaynor, Erin C., Cawthraw, Shaun, Manning, Georgina, MacKichan, Joanna K., Falkow, Stanley, Newell, Diane G.
The genome sequence of the enteric bacterial pathogen Campylobacter jejuni NCTC 11168 (11168-GS) was published in 2000, providing a valuable resource for the identification of C. jejuni-specific...
Restriction in Genetic Crosses Between Escherichia coli and Shigella flexneri
Falkow, Stanley, Formal, S. B.
Shigella flexneri restricts Escherichia coli deoxyribonucleic acid (DNA) and can modify phage DNA so that it is restricted in E. coli.
Protection of Monkeys Against Experimental Shigellosis with Attenuated Vaccines
Formal, Samuel B., LaBrec, E. H., Palmer, Amos, Falkow, Stanley
Formal, Samuel B. (Walter Reed Army Institute of Research, Washington, D.C.), E. H. LaBrec, Amos Palmer, and Stanley Falkow. Protection of monkeys against experimental shigellosis with attenuated...
Nucleic Acid Homologies of Selected Bacteria, L Forms, and Mycoplasma Species1
Rogul, M., McGee, Z. A., Wittler, R. G., Falkow, Stanley
Rogul, M. (Walter Reed Army Institute of Research, Washington, D.C.), Z. A. McGee, R. G. Wittler, and Stanley Falkow. Nucleic acid homologies of selected bacteria, L forms, and Mycoplasma species. J....
Leonard, Edward E., Tompkins, Lucy S., Falkow, Stanley, Nachamkin, Irving
We asked whether Campylobacter jejuni isolated from patients with Guillain-Barré syndrome (GBS) differ from isolates isolated from patients with uncomplicated gastrointestinal infection using DNA...
Plasmid-Mediated Beta-Lactamase Production in Neisseria gonorrhoeae
Elwell, Lynn P., Roberts, Marilyn, Mayer, Leonard W., Falkow, Stanley
Several β-lactamase-producing, penicillin-resistant strains of Neisseria gonorrhoeae were examined for R plasmids. Penicillin-resistant strains isolated from men returning from the Far East and...
Joyce, Elizabeth A., Kawale, Amita, Censini, Stefano, Kim, Charles C., Covacci, Antonello, Falkow, Stanley
Streptococcus pneumoniae causes several diseases, including otitis media, pneumonia, and meningitis. Although little is known about the regulation of or how individual pneumococcal factors contribute...
Relationship Between Colicin V Activity and Virulence in Escherichia coli
Quackenbush, Robert L., Falkow, Stanley
Colicin V activity is not essential to ColV plasmid-mediated virulence enhancement in Escherichia coli.
In Vivo Conjugal Transfer of R Plasmids in Neisseria gonorrhoeae
Roberts, Marilyn, Falkow, Stanley
In vivo R plasmid transfer between two Neisseria gonorrhoeae strains was detected in the absence of antibiotic pressure.
Plasmid-Associated Enterotoxin Production in a Strain of Escherichia coli Isolated from Humans
Skerman, F. J., Formal, S. B., Falkow, Stanley
A strain of Escherichia coli isolated from an epidemic of “nonspecific” diarrhea has been shown to harbor a plasmid which specifies enterotoxin production.
Characterization of Plasmid Deoxyribonucleic Acid from Neisseria gonorrhoeae
Mayer, Leonard W., Holmes, King K., Falkow, Stanley
Five of six independent gonococcal isolates have been found to harbor a plasmid pool of covalently closed-circular deoxyribonucleic acid about 2.4 × 106 daltons in size. The plasmid species were...
Kim, Charles C., Falkow, Stanley
Survival and replication in the intracellular environment are critical components of the ability of Salmonella enterica serovar Typhimurium to establish systemic infection in the murine host....
Thompson, Lucinda J., Danon, Stephen J., Wilson, John E., O'Rourke, Jani L., Salama, Nina R., Falkow, Stanley, ...
The mouse model of Helicobacter pylori-induced disease using Sydney strain 1 (SS1) has been used extensively in Helicobacter research. Herein we describe the isolation and characterization of a new...
Global Transposon Mutagenesis and Essential Gene Analysis of Helicobacter pylori†
Salama, Nina R., Shepherd, Benjamin, Falkow, Stanley
We have constructed a genome-saturating mutant library of the human gastric pathogen Helicobacter pylori. Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363...
Gaynor, Erin C., Cawthraw, Shaun, Manning, Georgina, MacKichan, Joanna K., Falkow, Stanley, Newell, Diane G.
Episomic Transfer between Salmonella Typhosa and Serratia Marcescens
Falkow, Stanley, Marmur, J., Carey, W. F., Spilman, W. M., Baron, L. S.
Chan, Kaman, Kim, Charles C., Falkow, Stanley
DNA microarrays provide an opportunity to combine the principles of signature-tagged mutagenesis (STM) with microarray technology to identify potentially important bacterial virulence genes. The...
Mueller, Anne, O’Rourke, Jani, Chu, Pauline, Chu, Amanda, Dixon, Michael F., Bouley, Donna M., ...
Gastric B-cell lymphoma of mucosa-associated lymphoid tissue type is closely linked to chronic Helicobacter pylori infection. Most clinical and histopathological features of the tumor can be...
Monack, Denise M., Bouley, Donna M., Falkow, Stanley
Host-adapted strains of Salmonella are capable of establishing a persistent infection in their host often in the absence of clinical disease. The mouse model of Salmonella infection has primarily...
Gerke, Christiane, Falkow, Stanley, Chien, Yueh-hsiu
T cell responses are critical to the survival of Yersinia-infected animals. Yersinia have the ability to directly suppress T lymphocyte activation through the virulence factor YopH, a tyrosine...
Salmonella Exploits Caspase-1 to Colonize Peyer's Patches in a Murine Typhoid Model
Monack, Denise M., Hersh, David, Ghori, Nafisa, Bouley, Donna, Zychlinsky, Arturo, Falkow, Stanley
Salmonella typhimurium invades host macrophages and induces apoptosis and the release of mature proinflammatory cytokines. SipB, a protein translocated by Salmonella into the cytoplasm of...
Suppression of T and B Lymphocyte Activation by a Yersinia pseudotuberculosis Virulence Factor, Yoph
Yao, Tony, Mecsas, Joan, Healy, James I., Falkow, Stanley, Chien, Yueh-hsiu
The acquired immune responses are crucial to the survival of Yersinia-infected animals. Mice lacking T cells are sensitive to Yersinia infection, and a humoral response to Yersinia can be protective....
Yersinia-induced Apoptosis In Vivo Aids in the Establishment of a Systemic Infection of Mice
Monack, Denise M., Mecsas, Joan, Bouley, Donna, Falkow, Stanley
Pathogenic Yersinia cause a systemic infection in mice that is dependent on the presence of a large plasmid encoding a number of secreted virulence proteins called Yops. We previously demonstrated...