Zheng, Ye, He, Xiao-Wei, Ying, Ying-Hui, Lu, Jiang-Feng, Gelvin, Stanton B., Shou, Hui-Xia
We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were...
Lee, Lan-Ying, Fang, Mei-Jane, Kuang, Lin-Yun, Gelvin, Stanton B
Abstract Background The investigation of protein-protein interactions is important for characterizing protein function. Bimolecular fluorescence complementation (BiFC) has recently gained interest as...
Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.
The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four...
An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration
Mysore, Kirankumar S., Nam, Jaesung, Gelvin, Stanton B.
Agrobacterium tumefaciens genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, the T-DNA, to the plant and integrating the T-DNA into the plant genome. Little is...
pSa Causes Oncogenic Suppression of Agrobacterium by Inhibiting VirE2 Protein Export
Lee, Lan-Ying, Gelvin, Stanton B., Kado, Clarence I.
When coresident with the Ti (tumor-inducing) plasmid, the 21-kDa product of the osa gene of the plasmid pSa can suppress crown gall tumorigenesis incited by Agrobacterium tumefaciens. Neither T-DNA...
Agrobacterium VirE2 Proteins Can Form a Complex with T Strands in the Plant Cytoplasm
Wild-type VirE2 and VirD2 proteins from Agrobacterium tumefaciens contain nuclear targeting sequences (NLS) that are likely involved in directing transferred T strands to the plant nucleus. An A....
Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of...
Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.
The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four ATP-dependent...
Identification of Arabidopsis rat Mutants
Zhu, Yanmin, Nam, Jaesung, Humara, Jaime M., Mysore, Kirankumar S., Lee, Lan-Ying, Cao, Hongbin, ...
Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to...
Zhu, Yanmin, Nam, Jaesung, Carpita, Nicholas C., Matthysse, Ann G., Gelvin, Stanton B.
Agrobacterium-mediated plant genetic transformation involves a complex interaction between the bacterium and the host plant. Relatively little is known about the role plant genes and proteins play in...
Tao, Yumin, Rao, Praveen K., Bhattacharjee, Saikat, Gelvin, Stanton B.
Agrobacterium tumefaciens transfers DNA to plant cells as a single-stranded DNA molecule (the T-strand) covalently linked to VirD2 protein. VirD2 contains nuclear localization signal sequences that...
Gaspar, Yolanda Maria, Nam, Jaesung, Schultz, Carolyn Jane, Lee, Lan-Ying, Gilson, Paul R., Gelvin, Stanton B., ...
Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens...
Lee, Lan-Ying, Gelvin, Stanton B.
The osa (oncogenic suppressive activity) gene of the IncW group plasmid pSa is sufficient to suppress tumorigenesis by Agrobacterium tumefaciens. osa confers oncogenic suppression by inhibiting VirE2...
Hwang, Hau-Hsuan, Gelvin, Stanton B.
Agrobacterium tumefaciens uses a type IV secretion system (T4SS) to transfer T-DNA and virulence proteins to plants. The T4SS is composed of two major structural components: the T-pilus and a...
The Synthesis of a 19 Kilodalton Zein Protein in Transgenic Petunia Plants 1
Williamson, John D., Galili, Gad, Larkins, Brian A., Gelvin, Stanton B.
Transcriptional fusions composed of a 19 kilodalton zein cDNA, the 5′ flanking region from a β-phaseolin gene, and 3′ flanking regions from either the phaseolin or a 15-kilodalton zein gene were...
Differential Accumulation of a Transcript Driven by the CaMV 35S Promoter in Transgenic Tobacco 1
Williamson, John D., Hirsch-Wyncott, Marlaya E., Larkins, Brian A., Gelvin, Stanton B.
A transcriptional fusion composed of the CaMV 35S promoter, a 19 kD zein cDNA, and the 3′ flanking regions from the 0′ to 1′ TR T-DNA genes was introduced into tobacco (Nicotiana tabacum) by...
Crown Gall Disease and Hairy Root Disease 1: A Sledgehammer and a Tackhammer
The neoplastic diseases crown gall and hairy root are incited by the phytopathogenic bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes, respectively. Although the molecular mechanism of...
Tian, Lu, Wang, Jianlin, Fong, M Paulus, Chen, Meng, Cao, Hongbin, Gelvin, Stanton B, ...
Little is known about the role of genetic and epigenetic control in the spatial and temporal regulation of plant development. Overexpressing antisense Arabidopsis thaliana HD1 (AtHD1) encoding a...
An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration
Mysore, Kirankumar S., Nam, Jaesung, Gelvin, Stanton B.
Agrobacterium tumefaciens genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, the T-DNA, to the plant and integrating the T-DNA into the plant genome. Little is...
pSa Causes Oncogenic Suppression of Agrobacterium by Inhibiting VirE2 Protein Export
Lee, Lan-Ying, Gelvin, Stanton B., Kado, Clarence I.
When coresident with the Ti (tumor-inducing) plasmid, the 21-kDa product of the osa gene of the plasmid pSa can suppress crown gall tumorigenesis incited by Agrobacterium tumefaciens. Neither T-DNA...
Agrobacterium VirE2 Proteins Can Form a Complex with T Strands in the Plant Cytoplasm
Wild-type VirE2 and VirD2 proteins from Agrobacterium tumefaciens contain nuclear targeting sequences (NLS) that are likely involved in directing transferred T strands to the plant nucleus. An A....
Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of...
Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.
The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four ATP-dependent...
Identification of Arabidopsis rat Mutants
Zhu, Yanmin, Nam, Jaesung, Humara, Jaime M., Mysore, Kirankumar S., Lee, Lan-Ying, Cao, Hongbin, ...
Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to...
Zhu, Yanmin, Nam, Jaesung, Carpita, Nicholas C., Matthysse, Ann G., Gelvin, Stanton B.
Agrobacterium-mediated plant genetic transformation involves a complex interaction between the bacterium and the host plant. Relatively little is known about the role plant genes and proteins play in...
Tao, Yumin, Rao, Praveen K., Bhattacharjee, Saikat, Gelvin, Stanton B.
Agrobacterium tumefaciens transfers DNA to plant cells as a single-stranded DNA molecule (the T-strand) covalently linked to VirD2 protein. VirD2 contains nuclear localization signal sequences that...
Gaspar, Yolanda Maria, Nam, Jaesung, Schultz, Carolyn Jane, Lee, Lan-Ying, Gilson, Paul R., Gelvin, Stanton B., ...
Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens...
Lee, Lan-Ying, Gelvin, Stanton B.
The osa (oncogenic suppressive activity) gene of the IncW group plasmid pSa is sufficient to suppress tumorigenesis by Agrobacterium tumefaciens. osa confers oncogenic suppression by inhibiting VirE2...
Hwang, Hau-Hsuan, Gelvin, Stanton B.
Agrobacterium tumefaciens uses a type IV secretion system (T4SS) to transfer T-DNA and virulence proteins to plants. The T4SS is composed of two major structural components: the T-pilus and a...
The Synthesis of a 19 Kilodalton Zein Protein in Transgenic Petunia Plants 1
Williamson, John D., Galili, Gad, Larkins, Brian A., Gelvin, Stanton B.
Transcriptional fusions composed of a 19 kilodalton zein cDNA, the 5′ flanking region from a β-phaseolin gene, and 3′ flanking regions from either the phaseolin or a 15-kilodalton zein gene were...
Differential Accumulation of a Transcript Driven by the CaMV 35S Promoter in Transgenic Tobacco 1
Williamson, John D., Hirsch-Wyncott, Marlaya E., Larkins, Brian A., Gelvin, Stanton B.
A transcriptional fusion composed of the CaMV 35S promoter, a 19 kD zein cDNA, and the 3′ flanking regions from the 0′ to 1′ TR T-DNA genes was introduced into tobacco (Nicotiana tabacum) by...
Crown Gall Disease and Hairy Root Disease 1: A Sledgehammer and a Tackhammer
The neoplastic diseases crown gall and hairy root are incited by the phytopathogenic bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes, respectively. Although the molecular mechanism of...
Tian, Lu, Wang, Jianlin, Fong, M Paulus, Chen, Meng, Cao, Hongbin, Gelvin, Stanton B, ...
Little is known about the role of genetic and epigenetic control in the spatial and temporal regulation of plant development. Overexpressing antisense Arabidopsis thaliana HD1 (AtHD1) encoding a...
Yi, HoChul, Sardesai, Nagesh, Fujinuma, Toshinori, Chan, Chien-Wei, Gelvin, Stanton B.
The Arabidopsis thaliana histone H2A gene HTA1 is essential for efficient transformation of Arabidopsis roots by Agrobacterium tumefaciens. Disruption of this gene in the rat5 mutant results in...
Crane, Yan Ma, Gelvin, Stanton B.
We investigated the effect of RNAi-mediated gene silencing of 109 Arabidopsis thaliana chromatin-related genes (termed “chromatin genes” hereafter) on Agrobacterium-mediated root transformation....
Novel Plant Transformation Vectors Containing the Superpromoter1[OA]
Lee, Lan-Ying, Kononov, Maria E., Bassuner, Burgund, Frame, Bronwyn R., Wang, Kan, Gelvin, Stanton B.
We developed novel plasmids and T-DNA binary vectors that incorporate a modified and more useful form of the superpromoter. The superpromoter consists of a trimer of the octopine synthase...
Hodges, Larry D., Lee, Lan-Ying, McNett, Henry, Gelvin, Stanton B., Ream, Walt
Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the...
Bhattacharjee, Saikat, Lee, Lan-Ying, Oltmanns, Heiko, Cao, Hongbin, Cuperus, Joshua, ...
Successful transformation of plants by Agrobacterium tumefaciens requires that the bacterial T-complex actively escorts T-DNA into the host's nucleus. VirD2 and VirE2 are virulence proteins on the...