Stefan Clerens

Direct profiling and identification of peptide expression differences in the pancreas of control and ob/ob mice by imaging mass spectrometry (2008)

Clerens, Stefan

Imaging mass spectrometry (IMS) technology utilizes MALDI MS to map molecules of interest in thin tissue sections. In this study, we have evaluated the potential of MALDI IMS to study peptide...

Anti-alpha-enolase antibodies in patients with inflammatory bowel disease (2008)

Clerens, Stefan, Van Den Bergh, Karolien, ...

BACKGROUND: Patients with inflammatory bowel disease (IBD) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies (pANCA). a-Enolase has been proposed as a target antigen in...

Peptides from the Bursa of Fabricius Associated with Suppression of Mitogen Stimulated DNA - Synthesis in Bursa of Fabricius Cells Belong to Intracellular Proteins (2008)

Stefan Clerens, Lut Arckens, Gisela F. Erf, G. Tellez, Billy M. Hargis

Previous reports from our laboratory have demonstrated that an reverse phase HPLC (rpHPLC) fraction obtained from extracts of the chicken bursa of Fabricius posses both in vitro anti-steroidogenic...

Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry (2007)

Ceuppens, Ruben, DUMONT, Debora, Van Brussel, Leen, Van De Plas, Babs, NOBEN, Jean-Paul, VERHAERT, Peter, ...

One of the newly developed imaging mass spectrometry (IMS) technologies utilizes matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to map proteins in thin tissue sections. In this...

Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry (2007)

DUMONT, Debora, Ceuppens, Ruben, Van Brussel, Leen, DANIELS, Ruth, NOBEN, Jean-Paul, Van De Plas, Babs, ...

One of the newly developed imaging mass spectrometry (IMS) technologies utilizes matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to map proteins in thin tissue sections. In this...

N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleft (2007)

Clerens, Stefan

* Recently, the three-dimensional structure of chicory (Cichorium intybus) fructan 1-exohydrolase (1-FEH IIa) in complex with its preferential substrate, 1-kestose, was determined. Unfortunately, no...

Cloning and functional analysis of a high DP fructan : fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes (2006)

Clerens, Stefan, Boogaerts, D, ...

Inulin-type fructans are the simplest and most studied fructans and have become increasingly popular as prebiotic health-improving compounds. A natural variation in the degree of polymerization (DP)...

Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.) (2006)

Nagaraj, V, Clerens, Stefan, Wiemken, A

Fructans, beta 2-1 and/or beta 2-6 linked polymers of fructose, are produced by fructosyltransferases (FTs) from sucrose. They are important storage carbohydrates in many plants. Fructan reserves,...

Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes (2006)

Van Den Ende, Wim, Clerens, Stefan, Vergauwen, Rudy, Boogaerts, David, Le Roy, Katrien, Arckens, Lutgarde, ...

Inulin-type fructans are the simplest and most studied fructans and have become increasingly popular as prebiotic health-improving compounds. A natural variation in the degree of polymerization (DP)...

Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.) (2006)

Van Riet, Liesbet, Nagaraj, Vinay, Van Den Ende, Wim, Clerens, Stefan, Wiemken, Andres, Van Laere, André

Fructans, β2-1 and/or β2-6 linked polymers of fructose, are produced by fructosyltransferases (FTs) from sucrose. They are important storage carbohydrates in many plants. Fructan reserves, widely...

Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes (2006)

Van Den Ende, Wim, Clerens, Stefan, Vergauwen, Rudy, Boogaerts, David, Le Roy, Katrien, Arckens, Lutgarde, ...

Inulin-type fructans are the simplest and most studied fructans and have become increasingly popular as prebiotic health-improving compounds. A natural variation in the degree of polymerization (DP)...

Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes (2006)

Van Den Ende, Wim, Clerens, Stefan, Vergauwen, Rudy, Boogaerts, David, Le Roy, Katrien, Arckens, Lutgarde, ...

Inulin-type fructans are the simplest and most studied fructans and have become increasingly popular as prebiotic health-improving compounds. A natural variation in the degree of polymerization (DP)...

Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities (2005)

Francis, I., Clerens, Stefan, Halliday, A.M., ...

The genome of Arabidopsis thaliana contains six putative cell-wall type invertase genes (AtcwINV1-6). Heterologous expression of AtcwINV1, 3 and 6 cDNAs in Pichia pastoris revealed that the enzymes...

Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.) (2005)

Van Riet, Liesbet, Nagaraj, Vinay, Van Den Ende, Wim, Clerens, Stefan, Wiemken, Andres, Van Laere, André

Fructans, β2-1 and/or β2-6 linked polymers of fructose, are produced by fructosyltransferases (FTs) from sucrose. They are important storage carbohydrates in many plants. Fructan reserves, widely...

Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.) (2005)

Van Riet, Liesbet, Nagaraj, Vinay, Van Den Ende, Wim, Clerens, Stefan, Wiemken, Andres, Van Laere, André

Fructans, β2-1 and/or β2-6 linked polymers of fructose, are produced by fructosyltransferases (FTs) from sucrose. They are important storage carbohydrates in many plants. Fructan reserves, widely...

Sweet Substitute: A software tool for in silico fragmentation of peptide-linked N-glycans (2004)

Clerens, Stefan, Van Den Ende, Wim; U0006486, Verhaert, P, Geenen, L

A software tool, Sweet Substitute, is described, which assists tandem mass spectrometry (MS/MS)-based glycosylation characterization from within a tryptic digest. The algorithm creates a virtual...

Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.) (2003)

Clerens, Stefan

About 15% of flowering plant species synthesize fructans. Fructans serve mainly as reserve carbohydrates and are subject to breakdown by plant fructan exohydrolases (FEHs), among which 1-FEHs...

Fructan 1-Exohydrolases. β-(2,1)-Trimmers during Graminan Biosynthesis in Stems of Wheat? Purification, Characterization, Mass Mapping, and Cloning of Two Fructan 1-Exohydrolase Isoforms1[w]

Van Den Ende, Wim, Clerens, Stefan, Vergauwen, Rudy, Van Riet, Liesbet, Van Laere, André, Yoshida, Midori, ...

Graminan-type fructans are temporarily stored in wheat (Triticum aestivum) stems. Two phases can be distinguished: a phase of fructan biosynthesis (green stems) followed by a breakdown phase (stems...

Identification of 26RFa, a hypothalamic neuropeptide of the RFamide peptide family with orexigenic activity

Chartrel, Nicolas, Dujardin, Cynthia, Anouar, Youssef, Leprince, Jérôme, Decker, Annick, Clerens, Stefan, ...

A neuropeptide was isolated from a frog brain extract by HPLC purification and characterized by mass spectrometry. This 26-aa neuropeptide, which belongs to the RFamide peptide family, was designated...

Fructan 1-Exohydrolases. β-(2,1)-Trimmers during Graminan Biosynthesis in Stems of Wheat? Purification, Characterization, Mass Mapping, and Cloning of Two Fructan 1-Exohydrolase Isoforms1[w]

Van Den Ende, Wim, Clerens, Stefan, Vergauwen, Rudy, Van Riet, Liesbet, Van Laere, André, Yoshida, Midori, ...

Graminan-type fructans are temporarily stored in wheat (Triticum aestivum) stems. Two phases can be distinguished: a phase of fructan biosynthesis (green stems) followed by a breakdown phase (stems...

Identification of 26RFa, a hypothalamic neuropeptide of the RFamide peptide family with orexigenic activity

Chartrel, Nicolas, Dujardin, Cynthia, Anouar, Youssef, Leprince, Jérôme, Decker, Annick, Clerens, Stefan, ...

A neuropeptide was isolated from a frog brain extract by HPLC purification and characterized by mass spectrometry. This 26-aa neuropeptide, which belongs to the RFamide peptide family, was designated...