Stefan Zeuzem

lHCV-related burden of disease in Europe: a systematic assessment of incidence, prevalence, morbidity, and mortality (2009)

Mühlberger, Nikolai, Schwarzer, Ruth, Lettmeier, Beate, Sroczynski, Gaby, Zeuzem, Stefan, Siebert, Uwe

Abstract Background Hepatitis C virus (HCV) is a leading cause of chronic liver disease, end-stage cirrhosis, and liver cancer, but little is known about the burden of disease caused by the virus. We...

Telaprevir and peginterferon with or without ribavirin for chronic HCV infection. (2009)

Hézode, Christophe, Forestier, Nicole, Dusheiko, Geoffrey, Ferenci, Peter, Pol, Stanislas, Goeser, Tobias, ...

BACKGROUND: In patients with chronic infection with hepatitis C virus (HCV) genotype 1, treatment with peginterferon alfa and ribavirin for 48 weeks results in rates of sustained virologic response...

Telaprevir and peginterferon with or without ribavirin for chronic HCV infection. (2009)

Hézode, Christophe, Forestier, Nicole, Dusheiko, Geoffrey, Ferenci, Peter, Pol, Stanislas, Goeser, Tobias, ...

BACKGROUND: In patients with chronic infection with hepatitis C virus (HCV) genotype 1, treatment with peginterferon alfa and ribavirin for 48 weeks results in rates of sustained virologic response...

Telaprevir and peginterferon with or without ribavirin for chronic HCV infection. (2009)

Hézode, Christophe, Forestier, Nicole, Dusheiko, Geoffrey, Ferenci, Peter, Pol, Stanislas, Goeser, Tobias, ...

BACKGROUND: In patients with chronic infection with hepatitis C virus (HCV) genotype 1, treatment with peginterferon alfa and ribavirin for 48 weeks results in rates of sustained virologic response...

Long-term effectiveness and cost-effectiveness of screening for Hepatitis C virus infection (2009)

Sroczynski, Gaby, Esteban, Eva, Conrads-Frank, Annette, Schwarzer, Ruth, Mühlberger, Nikolai, Wright, Davene, ...

Background: Hepatitis C virus (HCV) infection is an emerging problem in public health. In most countries, the majority of HCV infected people are yet undiagnosed. Early detection and treatment may...

Molecular basis of telaprevir resistance due to V36 and T54 mutations in the NS3-4A protease of the hepatitis C virus (2008)

Welsch, Christoph, Domingues, Francisco S, Susser, Simone, Antes, Iris, Hartmann, Christoph, Mayr, Gabriele, ...

Abstract Background The inhibitor telaprevir (VX-950) of the hepatitis C virus (HCV) protease NS3-4A has been tested in a recent phase 1b clinical trial in patients infected with HCV genotype 1. This...

Identification and in silico characterization of a novel compound heterozygosty associated with hereditary aceruloplasminemia (2007)

Hofmann, Wolf Peter, Welsch, Christoph, Takahashi, Yoshitomo, Miyajima, Hiroaki, Mihm, Ulrike, Krick, Christoph, ...

Background. Hereditary aceruloplasminemia is an adult-onset autosomal recessive disease characterized by increased iron overload in the liver, pancreas, retina, and central nervous system. So far, 45...

Biochemical and histological effects of 26 weeks of glycyrrhizin treatment in chronic hepatitis C: a randomized phase II trial (2006)

Hansen, Bettina E, Willems, Marc, Brouwer, Johannes T, Huber, Roman, Kullak-Ublick, Gerd A, ...

BACKGROUND/AIMS: Phase I/II studies of 4 weeks duration have confirmed the ALT lowering effect of glycyrrhizin in Western chronic hepatitis C patients. Our aim was to determine the dose frequency of...

Early monotherapy with pegylated interferon alpha-2b for acute hepatitis C infection: the HEP-NET acute-HCV-II study (2006)

Wiegand, Johannes, Buggisch, Peter, Boecher, Wulf, Zeuzem, Stefan, Gelbmann, Cornelia M., Berg, Thomas, ...

Early treatment of acute hepatitis C with interferon alpha-2b for 24 weeks prevents chronic infection in almost all patients. Because pegylated interferons have replaced conventional interferon in...

Mutations in the MutSalpha interaction interface of {MLH1} can abolish {DNA} mismatch repair (2006)

Plotz, Guido, Welsch, Christoph, Giron-Monzon, Luis, Friedhoff, Peter, Albrecht, Mario, Piiper, Albrecht, ...

MutL, a heterodimer of MLH1 and PMS2, plays a central role in human DNA mismatch repair. It interacts ATP-dependently with the mismatch detector MutS and assembles and controls further repair...

Peginterferon-α2a and ribavirin combination therapy in chronic hepatitis C. A randomized study of treatment duration and ribavirin dose (2004)

Hadziyannis, Stephanos J., Sette, Hoel, JrMorgan, Timothy R., Balan, Vijayan, Diago, Moises, Marcellin, Patrick, ...

BACKGROUND: Treatment with pegylated interferon (peginterferon) and ribavirin for 48 weeks is more effective than conventional interferon and ribavirin in patients with chronic hepatitis C....

N-terminus of hMLH1 confers interaction of hMutL{alpha} and hMutL{beta} with hMutS{alpha} (2003)

Plotz, Guido, Raedle, Jochen, Brieger, Angela, Trojan, Jörg, Zeuzem, Stefan

Mismatch repair is a highly conserved system that ensures replication fidelity by repairing mispairs after DNA synthesis. In humans, the two protein heterodimers hMutSα (hMSH2‐hMSH6) and...

hMutS{alpha} forms an ATP-dependent complex with hMutL{alpha} and hMutL{beta} on DNA (2002)

Plotz, Guido, Raedle, Jochen, Brieger, Angela, Trojan, Jörg, Zeuzem, Stefan

The DNA binding properties of hMutSα and hMutLα and complex formation of hMutSα with hMutLα and hMutL&bgr; were investigated using binding experiments on magnetic bead-coupled DNA substrates with...

Assessment, by Transcription-Mediated Amplification, of Virologic Response in Patients with Chronic Hepatitis C Virus Treated with Peginterferon α-2a

Sarrazin, Christoph, Hendricks, David A., Sedarati, Farhad, Zeuzem, Stefan

Transcription-mediated amplification (TMA) is an isothermal, autocatalytic target amplification method which has the potential to detect less than 50 hepatitis C virus (HCV) RNA copies/ml (10 IU/ml)....

hMutSα forms an ATP-dependent complex with hMutLα and hMutLβ on DNA

Plotz, Guido, Raedle, Jochen, Brieger, Angela, Trojan, Jörg, Zeuzem, Stefan

The DNA binding properties of hMutSα and hMutLα and complex formation of hMutSα with hMutLα and hMutLβ were investigated using binding experiments on magnetic bead-coupled DNA substrates with...

Hepatitis C Virus Nonstructural 5A Protein and Interferon Resistance: a New Model for Testing the Reliability of Mutational Analyses

Sarrazin, Christoph, Herrmann, Eva, Bruch, Katharina, Zeuzem, Stefan

Presumably due to the capability of the hepatitis C virus (HCV) to evade the antiviral effects of alpha interferon, treatment is ineffective in more than half of chronically genotype HCV type 1...

N-terminus of hMLH1 confers interaction of hMutLα and hMutLβ with hMutSα

Plotz, Guido, Raedle, Jochen, Brieger, Angela, Trojan, Jörg, Zeuzem, Stefan

Mismatch repair is a highly conserved system that ensures replication fidelity by repairing mispairs after DNA synthesis. In humans, the two protein heterodimers hMutSα (hMSH2-hMSH6) and hMutLα...

Epac Activation Converts cAMP from a Proliferative into a Differentiation Signal in PC12 CellsD⃞

Kiermayer, Simone, Biondi, Ricardo M., Imig, Jochen, Plotz, Guido, Haupenthal, Jörg, Zeuzem, Stefan, ...

Elevation of the intracellular cAMP concentration ([cAMP]i) regulates metabolism, cell proliferation, and differentiation and plays roles in memory formation and neoplastic growth. cAMP mediates its...

Comparison of Conventional PCR with Real-Time PCR and Branched DNA-Based Assays for Hepatitis C Virus RNA Quantification and Clinical Significance for Genotypes 1 to 5

Sarrazin, Christoph, Gärtner, Barbara C., Sizmann, Dorothea, Babiel, Rainer, Mihm, Ulrike, Hofmann, Wolf Peter, ...

The key parameter for diagnosis and management of hepatitis C virus (HCV) infection is HCV RNA. Standardization of HCV RNA assays to IU is mainly based on genotype 1 panels. Little is known about the...

Assessment, by Transcription-Mediated Amplification, of Virologic Response in Patients with Chronic Hepatitis C Virus Treated with Peginterferon α-2a

Sarrazin, Christoph, Hendricks, David A., Sedarati, Farhad, Zeuzem, Stefan

Transcription-mediated amplification (TMA) is an isothermal, autocatalytic target amplification method which has the potential to detect less than 50 hepatitis C virus (HCV) RNA copies/ml (10 IU/ml)....

hMutSα forms an ATP-dependent complex with hMutLα and hMutLβ on DNA

Plotz, Guido, Raedle, Jochen, Brieger, Angela, Trojan, Jörg, Zeuzem, Stefan

The DNA binding properties of hMutSα and hMutLα and complex formation of hMutSα with hMutLα and hMutLβ were investigated using binding experiments on magnetic bead-coupled DNA substrates with...

Hepatitis C Virus Nonstructural 5A Protein and Interferon Resistance: a New Model for Testing the Reliability of Mutational Analyses

Sarrazin, Christoph, Herrmann, Eva, Bruch, Katharina, Zeuzem, Stefan

Presumably due to the capability of the hepatitis C virus (HCV) to evade the antiviral effects of alpha interferon, treatment is ineffective in more than half of chronically genotype HCV type 1...

N-terminus of hMLH1 confers interaction of hMutLα and hMutLβ with hMutSα

Plotz, Guido, Raedle, Jochen, Brieger, Angela, Trojan, Jörg, Zeuzem, Stefan

Mismatch repair is a highly conserved system that ensures replication fidelity by repairing mispairs after DNA synthesis. In humans, the two protein heterodimers hMutSα (hMSH2-hMSH6) and hMutLα...

Epac Activation Converts cAMP from a Proliferative into a Differentiation Signal in PC12 CellsD⃞

Kiermayer, Simone, Biondi, Ricardo M., Imig, Jochen, Plotz, Guido, Haupenthal, Jörg, Zeuzem, Stefan, ...

Elevation of the intracellular cAMP concentration ([cAMP]i) regulates metabolism, cell proliferation, and differentiation and plays roles in memory formation and neoplastic growth. cAMP mediates its...

Comparison of Conventional PCR with Real-Time PCR and Branched DNA-Based Assays for Hepatitis C Virus RNA Quantification and Clinical Significance for Genotypes 1 to 5

Sarrazin, Christoph, Gärtner, Barbara C., Sizmann, Dorothea, Babiel, Rainer, Mihm, Ulrike, Hofmann, Wolf Peter, ...

The key parameter for diagnosis and management of hepatitis C virus (HCV) infection is HCV RNA. Standardization of HCV RNA assays to IU is mainly based on genotype 1 panels. Little is known about the...

Mutations in the MutSα interaction interface of MLH1 can abolish DNA mismatch repair

Plotz, Guido, Welsch, Christoph, Giron-Monzon, Luis, Friedhoff, Peter, Albrecht, Mario, Piiper, Albrecht, ...

MutLα, a heterodimer of MLH1 and PMS2, plays a central role in human DNA mismatch repair. It interacts ATP-dependently with the mismatch detector MutSα and assembles and controls further repair...

Allosteric activation of the protein kinase PDK1 with low molecular weight compounds

Engel, Matthias, Hindie, Valerie, Lopez-Garcia, Laura A, Stroba, Adriana, Schaeffer, Francis, Adrian, Iris, ...

Organisms rely heavily on protein phosphorylation to transduce intracellular signals. The phosphorylation of a protein often induces conformational changes, which are responsible for triggering...

Peginterferon alpha-2a (40kD) [Pegasys(R)] Improves HR-QOL Outcomes Compared with Unmodified Interferon alpha-2a [Roferon(R)-A]: In Patients with Chronic Hepatitis C

Jens Rasenack, Stefan Zeuzem, S. Victor Feinman, E. Jenny Heathcote, Michael Manns, Eric M. Yoshida, ...

Background: Use of unmodified interferon alpha-2a in chronic hepatitis C is associated with impaired health-related quality of life during therapy. Treatment with peginterferon alpha-2a (40kD)...

Molecular basis of telaprevir resistance due to V36 and T54 mutations in the NS3-4A protease of the hepatitis C virus

Welsch, Christoph, Domingues, Francisco S, Susser, Simone, Antes, Iris, Hartmann, Christoph, Mayr, Gabriele, ...

Structural analysis of the inhibitor Telaprevir (VX-950) of the hepatitis C virus (HCV) protease NS3-4A shows that mutations at V36 and/or T54 result in impaired interaction with VX-950, explaining...