Non-pathogenic trypanosomatid protozoa as a platform for protein research and production (2002)
Breitling,Reinhard, Klingner,Susanne, Callewaert,Nico, Pietrucha,Regina, Geyer,Anett, Ehrlich,Gunter, ...
All currently existing eukaryotic protein expression systems are based on autonomous life forms. To exploit the potential practical benefits associated with parasitic organisms we have developed a...
Non-pathogenic trypanosomatid protozoa as a platform for protein research and production (2002)
Breitling, Reinhard, Klingner, Susanne, Callewaert, Nico, Pietrucha, Regina, Geyer, Anett, Ehrlich, Gunter, ...
All currently existing eukaryotic protein expression systems are based on autonomous life forms. To exploit the potential practical benefits associated with parasitic organisms we have developed a...
Gourley, David G., Schuttelkopf, Alexander W., Leonard, Gordon A., Luba, James, Hardy, Larry W., Beverley, Stephen M., ...
Pteridine reductase (PTR1) is a short-chain reductase (SDR) responsible for the salvage of pterins in parasitic trypanosomatids. PTR1 catalyzes the NADPH-dependent two-step reduction of oxidized...
Crystallization of recombinant Leishmania major pteridine reductase 1 (PTR1) (1999)
Gourley, David G., Luba, James, Hardy, Larry W., Beverley, Stephen M., Hunter, William N.
The enzyme pteridine reductase (PTR1) has recently been discovered in the protozoan parasite Leishmania and validated as a target for therapeutic intervention. PTR1 is responsible for the salvage of...
Luba, James, Nare, Bakela, Liang, Po-Huang, Anderson, Karen S., Beverley, Stephen M., Hardy, Larry W.
Pteridine reductase 1 (PTR1) is a novel broad spectrum enzyme of pterin and folate metabolism in the protozoan parasite Leishmania. Overexpression of PTR1 confers methotrexate resistance to these...
Nare, Bakela, Luba, James, Hardy, Larry W., Beverley, Stephen M.
Leishmania and other trypanosomatid protozoa require reduced pteridines (pterins and folates) for growth, suggesting that inhibition of these pathways could be targeted for effective chemotherapy....
Späth, Gerald F., Epstein, Linda, Leader, Ben, Singer, Steven M., Avila, Herbert A., Turco, Salvatore J., ...
Protozoan parasites of the genus Leishmania undergo a complex life cycle involving transmission by biting sand flies and replication within mammalian macrophage phagolysosomes. A major component of...
Gehring, Amy M., Nodwell, Justin R., Beverley, Stephen M., Losick, Richard
The filamentous soil bacterium Streptomyces coelicolor undergoes a complex cycle of morphological differentiation involving the formation of an aerial mycelium and the production of pigmented...
Mamoun, Choukri Ben, Gluzman, Ilya Y., Goyard, Sophie, Beverley, Stephen M., Goldberg, Daniel E.
Genomic information is rapidly accumulating for the human malaria pathogen, Plasmodium falciparum. Our ability to perform genetic manipulations to understand Plasmodium gene function is limited....
Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant
Vasudevan, Gayatri, Carter, Nicola S., Drew, Mark E., Beverley, Stephen M., Sanchez, Marco A., Seyfang, Andreas, ...
All parasitic protozoa studied to date are incapable of purine biosynthesis and must therefore salvage purine nucleobases or nucleosides from their hosts. This salvage process is initiated by purine...
The role of phosphoglycans in Leishmania–sand fly interactions
Sacks, David L., Modi, Govind, Rowton, Edgar, Späth, Gerald, Epstein, Linda, Turco, Salvatore J., ...
Leishmania promastigotes synthesize an abundance of phosphoglycans, either attached to the cell surface through phosphatidylinositol anchors (lipophosphoglycan, LPG) or secreted as protein-containing...
Chakkalath, Hrishekesh R., Siddiqui, Afzal A., Shankar, Anuraj H., Dobson, Deborah E., Beverley, Stephen M., Titus, Richard G.
To determine whether an ongoing response to Leishmania major would affect the response to a non-cross-reacting, non-leishmanial antigen, susceptible BALB/c mice and resistant C3H mice were infected...
Heterologous Expression of Trypanosoma cruzi trans-Sialidase in Leishmania major Enhances Virulence
Belen Carrillo, M., Gao, Wenda, Herrera, Macario, Alroy, Joseph, Moore, Jeffrey B., Beverley, Stephen M., ...
Earlier studies showed that mice primed for a few hours with the trans-sialidase (TS) of Trypanosoma cruzi, the agent of Chagas' disease, become highly susceptible to trypanosomal infection. These...
Tosi, Luiz R. O., Beverley, Stephen M.
Mos1 and other mariner/Tc1 transposons move horizontally during evolution, and when transplanted into heterologous species can transpose in organisms ranging from prokaryotes to protozoans and...
Descoteaux, Albert, Avila, Herbert A., Zhang, Kai, Turco, Salvatore J., Beverley, Stephen M.
Leishmania promastigotes express an abundant cell surface glycoconjugate, lipophosphoglycan (LPG). LPG contains a polymer of the disaccharide-phosphate repeat unit Galβ1,4Manα1-PO4, shared by other...
Gao, Lian-Yong, Groger, Richard, Cox, Jeffery S., Beverley, Stephen M., Lawson, Elise H., Brown, Eric J.
Pathogenic mycobacteria survive and replicate within host macrophages, but the molecular mechanisms involved in this necessary step in the pathogenesis of infection are not completely understood....
Späth, Gerald F., Garraway, L. A., Turco, Salvatore J., Beverley, Stephen M.
The abundant cell surface glycolipid lipophosphoglycan (LPG) was implicated in many steps of the Leishmania infectious cycle by biochemical tests. The presence of other abundant surface or secreted...
Sphingolipids are essential for differentiation but not growth in Leishmania
Zhang, Kai, Showalter, Melissa, Revollo, Javier, Hsu, Fong-Fu, Turk, John, Beverley, Stephen M.
Sphingolipids (SLs) play critical roles in eukaryotic cells in the formation of lipid rafts, membrane trafficking, and signal transduction. Here we created a SL null mutant in the protozoan parasite...
Späth, Gerald F., Lye, Lon-Fye, Segawa, Hiroaki, Turco, Salvatore J., Beverley, Stephen M.
Different Leishmania species rely to different extents on abundant glycoconjugates, such as lipophosphoglycan (LPG) and related molecules, in mammalian infections. Previously, we showed that...
Boulanger, Nathalie, Lowenberger, Carl, Volf, Petr, Ursic, Raul, Sigutova, Lucie, Sabatier, Laurence, ...
Antimicrobial peptides are major components of the innate immune response of epithelial cells. In insect vectors, these peptides may play a role in the control of gut pathogens. We have analyzed...
Eukaryotic UDP-Galactopyranose Mutase (GLF Gene) in Microbial and Metazoal Pathogens†
Beverley, Stephen M., Owens, Katherine L., Showalter, Melissa, Griffith, Cara L., Doering, Tamara L., Jones, Victoria C., ...
Galactofuranose (Galf) is a novel sugar absent in mammals but present in a variety of pathogenic microbes, often within glycoconjugates that play critical roles in cell surface formation and the...
Kébaïer, Chahnaz, Uzonna, Jude E., Beverley, Stephen M., Scott, Phillip
Leishmania major parasites lacking the GDP-mannose transporter, termed Δlpg2 parasites, fail to induce disease in mice but persist long-term. We previously found that Δlpg2 organisms protect BALB/c...
The Genome of the Kinetoplastid Parasite, Leishmania major
Ivens, Alasdair C., Peacock, Christopher S., Worthey, Elizabeth A., Murphy, Lee, Aggarwal, Gautam, Berriman, Matthew, ...
Späth, Gerald F., Epstein, Linda, Leader, Ben, Singer, Steven M., Avila, Herbert A., Turco, Salvatore J., ...
Protozoan parasites of the genus Leishmania undergo a complex life cycle involving transmission by biting sand flies and replication within mammalian macrophage phagolysosomes. A major component of...
Gehring, Amy M., Nodwell, Justin R., Beverley, Stephen M., Losick, Richard
The filamentous soil bacterium Streptomyces coelicolor undergoes a complex cycle of morphological differentiation involving the formation of an aerial mycelium and the production of pigmented...
Mamoun, Choukri Ben, Gluzman, Ilya Y., Goyard, Sophie, Beverley, Stephen M., Goldberg, Daniel E.
Genomic information is rapidly accumulating for the human malaria pathogen, Plasmodium falciparum. Our ability to perform genetic manipulations to understand Plasmodium gene function is limited....
Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant
Vasudevan, Gayatri, Carter, Nicola S., Drew, Mark E., Beverley, Stephen M., Sanchez, Marco A., Seyfang, Andreas, ...
All parasitic protozoa studied to date are incapable of purine biosynthesis and must therefore salvage purine nucleobases or nucleosides from their hosts. This salvage process is initiated by purine...
The role of phosphoglycans in Leishmania–sand fly interactions
Sacks, David L., Modi, Govind, Rowton, Edgar, Späth, Gerald, Epstein, Linda, Turco, Salvatore J., ...
Leishmania promastigotes synthesize an abundance of phosphoglycans, either attached to the cell surface through phosphatidylinositol anchors (lipophosphoglycan, LPG) or secreted as protein-containing...
Chakkalath, Hrishekesh R., Siddiqui, Afzal A., Shankar, Anuraj H., Dobson, Deborah E., Beverley, Stephen M., Titus, Richard G.
To determine whether an ongoing response to Leishmania major would affect the response to a non-cross-reacting, non-leishmanial antigen, susceptible BALB/c mice and resistant C3H mice were infected...
Heterologous Expression of Trypanosoma cruzi trans-Sialidase in Leishmania major Enhances Virulence
Belen Carrillo, M., Gao, Wenda, Herrera, Macario, Alroy, Joseph, Moore, Jeffrey B., Beverley, Stephen M., ...
Earlier studies showed that mice primed for a few hours with the trans-sialidase (TS) of Trypanosoma cruzi, the agent of Chagas' disease, become highly susceptible to trypanosomal infection. These...
Tosi, Luiz R. O., Beverley, Stephen M.
Mos1 and other mariner/Tc1 transposons move horizontally during evolution, and when transplanted into heterologous species can transpose in organisms ranging from prokaryotes to protozoans and...
Descoteaux, Albert, Avila, Herbert A., Zhang, Kai, Turco, Salvatore J., Beverley, Stephen M.
Leishmania promastigotes express an abundant cell surface glycoconjugate, lipophosphoglycan (LPG). LPG contains a polymer of the disaccharide-phosphate repeat unit Galβ1,4Manα1-PO4, shared by other...
Gao, Lian-Yong, Groger, Richard, Cox, Jeffery S., Beverley, Stephen M., Lawson, Elise H., Brown, Eric J.
Pathogenic mycobacteria survive and replicate within host macrophages, but the molecular mechanisms involved in this necessary step in the pathogenesis of infection are not completely understood....
Späth, Gerald F., Garraway, L. A., Turco, Salvatore J., Beverley, Stephen M.
The abundant cell surface glycolipid lipophosphoglycan (LPG) was implicated in many steps of the Leishmania infectious cycle by biochemical tests. The presence of other abundant surface or secreted...
Sphingolipids are essential for differentiation but not growth in Leishmania
Zhang, Kai, Showalter, Melissa, Revollo, Javier, Hsu, Fong-Fu, Turk, John, Beverley, Stephen M.
Sphingolipids (SLs) play critical roles in eukaryotic cells in the formation of lipid rafts, membrane trafficking, and signal transduction. Here we created a SL null mutant in the protozoan parasite...
Späth, Gerald F., Lye, Lon-Fye, Segawa, Hiroaki, Turco, Salvatore J., Beverley, Stephen M.
Different Leishmania species rely to different extents on abundant glycoconjugates, such as lipophosphoglycan (LPG) and related molecules, in mammalian infections. Previously, we showed that...
Boulanger, Nathalie, Lowenberger, Carl, Volf, Petr, Ursic, Raul, Sigutova, Lucie, Sabatier, Laurence, ...
Antimicrobial peptides are major components of the innate immune response of epithelial cells. In insect vectors, these peptides may play a role in the control of gut pathogens. We have analyzed...
Eukaryotic UDP-Galactopyranose Mutase (GLF Gene) in Microbial and Metazoal Pathogens†
Beverley, Stephen M., Owens, Katherine L., Showalter, Melissa, Griffith, Cara L., Doering, Tamara L., Jones, Victoria C., ...
Galactofuranose (Galf) is a novel sugar absent in mammals but present in a variety of pathogenic microbes, often within glycoconjugates that play critical roles in cell surface formation and the...
Kébaïer, Chahnaz, Uzonna, Jude E., Beverley, Stephen M., Scott, Phillip
Leishmania major parasites lacking the GDP-mannose transporter, termed Δlpg2 parasites, fail to induce disease in mice but persist long-term. We previously found that Δlpg2 organisms protect BALB/c...
Beverley, Stephen M, Akopyants, Natalia S, Goyard, Sophie, Matlib, Robin S, Gordon, Jennifer L, Brownstein, Bernard H, ...
Leishmania are important protozoan pathogens of humans in temperate and tropical regions. The study of gene expression during the infectious cycle, in mutants or after environmental or chemical...
Capul, Althea A., Hickerson, Suzanne, Barron, Tamara, Turco, Salvatore J., Beverley, Stephen M.
Abundant surface Leishmania phosphoglycans (PGs) containing [Gal(β1,4)Man(α1-PO4)]-derived repeating units are important at several points in the infectious cycle of this protozoan parasite. PG...
Redirection of sphingolipid metabolism toward de novo synthesis of ethanolamine in Leishmania
Zhang, Kai, Pompey, Justine M, Hsu, Fong-Fu, Key, Phillip, Bandhuvula, Padmavathi, Saba, Julie D, ...
In most eukaryotes, sphingolipids (SLs) are critical membrane components and signaling molecules. However, mutants of the trypanosomatid protozoan Leishmania lacking serine palmitoyltransferase...
Migratory Dermal Dendritic Cells Act as Rapid Sensors of Protozoan Parasites
Ng, Lai Guan, Hsu, Alice, Mandell, Michael A., Roediger, Ben, Hoeller, Christoph, Mrass, Paulus, ...
Dendritic cells (DC), including those of the skin, act as sentinels for intruding microorganisms. In the epidermis, DC (termed Langerhans cells, LC) are sessile and screen their microenvironment...