Wychowski, Czeslaw, Emerson, Suzanne U., Silver, Jonathan, Feinstone, Stephen M.
In order to study the importance of VP4 in picornavirus replication and translation, we replaced the hepatitis A virus (HAV) VP4 with the poliovirus (PV1) VP4. Using a modification of oligonucleotide...
TASSOPOULOS, NICOLAOS C., PAPAEVANGELOU, GEORGE J., ROUMELIOTOU-KARAYANNIS, ANASTASIA, TICEHURST, JOHN R., FEINSTONE, STEPHEN M., PURCELL, ROBERT H.
Tassopoulos, N. C, G. J. Papaevangelou (National Center for Viral Hepatitis, Athens, Greece), A. Roumeliotou-Karayannis, J. R. Ticehurst, S. M. Feinstone, and R. H. Purcell. Detection of hepatitis B...
Molecular cloning and characterization of hepatitis A virus cDNA (1983)
Ticehurst, John R., Racaniello, Vincent R., Baroudy, Bahige M., Baltimore, David, Purcell, Robert H., Feinstone, Stephen M.
Double-stranded cDNA was synthesized from hepatitis A virus (HAV) RNA and inserted into the Pst I site of pBR322. Restriction endonuclease digestion and cross-hybridization of fragments yielded a map...
Arichi, Tatsumi, Saito, Takafumi, Major, Marian E., Belyakov, Igor M., Shirai, Mutsunori, Engelhard, Victor H., ...
DNA vaccines express antigens intracellularly and effectively induce cellular immune responses. Because only chimpanzees can be used to model human hepatitis C virus (HCV) infections, we developed a...
Arichi, Tatsumi, Saito, Takafumi, Major, Marian E., Belyakov, Igor M., Shirai, Mutsunori, Engelhard, Victor H., ...
Long-Term Follow-Up of Chimpanzees Inoculated with the First Infectious Clone for Hepatitis C Virus
Major, Marian E., Mihalik, Kathleen, Fernandez, Javier, Seidman, Jessica, Kleiner, David, Kolykhalov, Alexander A., ...
Two chimpanzees (Ch1535 and Ch1536) became infected with hepatitis C virus (HCV) following intrahepatic inoculation with RNA transcribed from a full-length cDNA clone of the virus. Both animals were...
Kolykhalov, Alexander A., Mihalik, Kathy, Feinstone, Stephen M., Rice, Charles M.
Hepatitis C virus (HCV) infection is a widespread major human health concern. Significant obstacles in the study of this virus include the absence of a reliable tissue culture system and a...
Mizukoshi, Eishiro, Nascimbeni, Michelina, Blaustein, Joshua B., Mihalik, Kathleen, Rice, Charles M., Liang, T. Jake, ...
The chimpanzee is a critical animal model for studying cellular immune responses to infectious pathogens such as hepatitis B and C viruses, human immunodeficiency virus, and malaria. Several...
Major, Marian E., Mihalik, Kathleen, Puig, Montserrat, Rehermann, Barbara, Nascimbeni, Michelina, Rice, Charles M., ...
Responses in three chimpanzees were compared following challenge with a clonal hepatitis C virus (HCV) contained in plasma from an animal that had received infectious RNA transcripts. Two of the...
Thomson, Michael, Nascimbeni, Michelina, Havert, Michael B., Major, Marian, Gonzales, Sophia, Alter, Harvey, ...
Clearance of hepatitis C virus (HCV) infection in humans and chimpanzees is thought to be associated with the induction of strong T-cell responses. We studied four chimpanzees infected with HCV...
Pavio, Nicole, Romano, Patrick R., Graczyk, Thomas M., Feinstone, Stephen M., Taylor, Deborah R.
The hepatitis C virus envelope protein, E2, is an endoplasmic reticulum (ER)-bound protein that contains a region of sequence homology with the double-stranded RNA-activated protein kinase PKR and...
Nascimbeni, Michelina, Mizukoshi, Eishiro, Bosmann, Markus, Major, Marian E., Mihalik, Kathleen, Rice, Charles M., ...
The immunological correlates of hepatitis C virus (HCV)-specific immunity are not well understood. Antibodies to HCV structural proteins do not appear to play a key role in clearance of the virus and...
Mathiesen, Lars R., Feinstone, Stephen M., Wong, Doris C., Skinhoej, Peter, Purcell, Robert H.
Previously described techniques for detection of hepatitis A antigen (HA Ag) and antibody (anti-HA) have required purified HA Ag and expensive equipment. Herein is described an enzyme-linked...
Yu, Mei-ying W., Bartosch, Birke, Zhang, Pei, Guo, Zheng-ping, Renzi, Paula M., Shen, Li-ming, ...
The role of humoral immunity in hepatitis C virus (HCV) infections is uncertain. Nevertheless, there is increasing evidence for neutralizing antibodies to HCV in the serum or plasma of chronically...
Fernandez, Javier, Taylor, Deborah, Morhardt, Duncan R., Mihalik, Kathleen, Puig, Montserrat, Rice, Charles M., ...
Two chimpanzees, 1535 and 1536, became persistently infected following inoculation with RNA transcripts from cDNA clones of hepatitis C virus (HCV). Analysis of the HCV genomes from both animals...
New Antiviral Pathway That Mediates Hepatitis C Virus Replicon Interferon Sensitivity through ADAR1
Taylor, Deborah R., Puig, Montserrat, Darnell, Miriam E. R., Mihalik, Kathleen, Feinstone, Stephen M.
While many clinical hepatitis C virus (HCV) infections are resistant to alpha interferon (IFN-α) therapy, subgenomic in vitro self-replicating HCV RNAs (HCV replicons) are characterized by marked...
Liver-Directed Gamma Interferon Gene Delivery in Chronic Hepatitis C
Shin, Eui-Cheol, Protzer, Ulrike, Untergasser, Andreas, Feinstone, Stephen M., Rice, Charles M., Hasselschwert, Dana, ...
Gamma interferon (IFN-γ) has been shown to inhibit replication of subgenomic and genomic hepatitis C virus (HCV) RNAs in vitro and to noncytolytically suppress hepatitis B virus (HBV) replication in...
Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro
Lindenbach, Brett D., Meuleman, Philip, Ploss, Alexander, Vanwolleghem, Thomas, Syder, Andrew J., McKeating, Jane A., ...
Hepatitis C virus (HCV) is a major cause of chronic liver disease, frequently progressing to cirrhosis and increased risk of hepatocellular carcinoma. Current therapies are inadequate and progress in...
Virus-induced type I IFN stimulates generation of immunoproteasomes at the site of infection
Shin, Eui-Cheol, Seifert, Ulrike, Kato, Takanobu, Rice, Charles M., Feinstone, Stephen M., Kloetzel, Peter-M., ...
IFN-γ is known as the initial and primary inducer of immunoproteasomes during viral infections. We now report that type I IFN induced the transcription and translation of immunoproteasome subunits,...
Arichi, Tatsumi, Saito, Takafumi, Major, Marian E., Belyakov, Igor M., Shirai, Mutsunori, Engelhard, Victor H., ...
DNA vaccines express antigens intracellularly and effectively induce cellular immune responses. Because only chimpanzees can be used to model human hepatitis C virus (HCV) infections, we developed a...
Arichi, Tatsumi, Saito, Takafumi, Major, Marian E., Belyakov, Igor M., Shirai, Mutsunori, Engelhard, Victor H., ...
Long-Term Follow-Up of Chimpanzees Inoculated with the First Infectious Clone for Hepatitis C Virus
Major, Marian E., Mihalik, Kathleen, Fernandez, Javier, Seidman, Jessica, Kleiner, David, Kolykhalov, Alexander A., ...
Two chimpanzees (Ch1535 and Ch1536) became infected with hepatitis C virus (HCV) following intrahepatic inoculation with RNA transcribed from a full-length cDNA clone of the virus. Both animals were...
Kolykhalov, Alexander A., Mihalik, Kathy, Feinstone, Stephen M., Rice, Charles M.
Hepatitis C virus (HCV) infection is a widespread major human health concern. Significant obstacles in the study of this virus include the absence of a reliable tissue culture system and a...
Mizukoshi, Eishiro, Nascimbeni, Michelina, Blaustein, Joshua B., Mihalik, Kathleen, Rice, Charles M., Liang, T. Jake, ...
The chimpanzee is a critical animal model for studying cellular immune responses to infectious pathogens such as hepatitis B and C viruses, human immunodeficiency virus, and malaria. Several...
Major, Marian E., Mihalik, Kathleen, Puig, Montserrat, Rehermann, Barbara, Nascimbeni, Michelina, Rice, Charles M., ...
Responses in three chimpanzees were compared following challenge with a clonal hepatitis C virus (HCV) contained in plasma from an animal that had received infectious RNA transcripts. Two of the...
Thomson, Michael, Nascimbeni, Michelina, Havert, Michael B., Major, Marian, Gonzales, Sophia, Alter, Harvey, ...
Clearance of hepatitis C virus (HCV) infection in humans and chimpanzees is thought to be associated with the induction of strong T-cell responses. We studied four chimpanzees infected with HCV...
Pavio, Nicole, Romano, Patrick R., Graczyk, Thomas M., Feinstone, Stephen M., Taylor, Deborah R.
The hepatitis C virus envelope protein, E2, is an endoplasmic reticulum (ER)-bound protein that contains a region of sequence homology with the double-stranded RNA-activated protein kinase PKR and...
Nascimbeni, Michelina, Mizukoshi, Eishiro, Bosmann, Markus, Major, Marian E., Mihalik, Kathleen, Rice, Charles M., ...
The immunological correlates of hepatitis C virus (HCV)-specific immunity are not well understood. Antibodies to HCV structural proteins do not appear to play a key role in clearance of the virus and...
Mathiesen, Lars R., Feinstone, Stephen M., Wong, Doris C., Skinhoej, Peter, Purcell, Robert H.
Previously described techniques for detection of hepatitis A antigen (HA Ag) and antibody (anti-HA) have required purified HA Ag and expensive equipment. Herein is described an enzyme-linked...
Yu, Mei-ying W., Bartosch, Birke, Zhang, Pei, Guo, Zheng-ping, Renzi, Paula M., Shen, Li-ming, ...
The role of humoral immunity in hepatitis C virus (HCV) infections is uncertain. Nevertheless, there is increasing evidence for neutralizing antibodies to HCV in the serum or plasma of chronically...
Fernandez, Javier, Taylor, Deborah, Morhardt, Duncan R., Mihalik, Kathleen, Puig, Montserrat, Rice, Charles M., ...
Two chimpanzees, 1535 and 1536, became persistently infected following inoculation with RNA transcripts from cDNA clones of hepatitis C virus (HCV). Analysis of the HCV genomes from both animals...
New Antiviral Pathway That Mediates Hepatitis C Virus Replicon Interferon Sensitivity through ADAR1
Taylor, Deborah R., Puig, Montserrat, Darnell, Miriam E. R., Mihalik, Kathleen, Feinstone, Stephen M.
While many clinical hepatitis C virus (HCV) infections are resistant to alpha interferon (IFN-α) therapy, subgenomic in vitro self-replicating HCV RNAs (HCV replicons) are characterized by marked...
Liver-Directed Gamma Interferon Gene Delivery in Chronic Hepatitis C
Shin, Eui-Cheol, Protzer, Ulrike, Untergasser, Andreas, Feinstone, Stephen M., Rice, Charles M., Hasselschwert, Dana, ...
Gamma interferon (IFN-γ) has been shown to inhibit replication of subgenomic and genomic hepatitis C virus (HCV) RNAs in vitro and to noncytolytically suppress hepatitis B virus (HBV) replication in...
Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro
Lindenbach, Brett D., Meuleman, Philip, Ploss, Alexander, Vanwolleghem, Thomas, Syder, Andrew J., McKeating, Jane A., ...
Hepatitis C virus (HCV) is a major cause of chronic liver disease, frequently progressing to cirrhosis and increased risk of hepatocellular carcinoma. Current therapies are inadequate and progress in...
Virus-induced type I IFN stimulates generation of immunoproteasomes at the site of infection
Shin, Eui-Cheol, Seifert, Ulrike, Kato, Takanobu, Rice, Charles M., Feinstone, Stephen M., Kloetzel, Peter-M., ...
IFN-γ is known as the initial and primary inducer of immunoproteasomes during viral infections. We now report that type I IFN induced the transcription and translation of immunoproteasome subunits,...
Evidence for a functional RNA element in the hepatitis C virus core gene
McMullan, Laura K., Grakoui, Arash, Evans, Matthew J., Mihalik, Kathleen, Puig, Montserrat, Branch, Andrea D., ...
In the core protein-coding region of hepatitis C virus (HCV), evidence exists for both phylogenetically conserved RNA structures and a +1 alternative reading frame (ARF). To investigate its role in...
Hepatitis C virus epitope-specific neutralizing antibodies in Igs prepared from human plasma
Zhang, Pei, Wu, Charles G., Mihalik, Kathleen, Virata-Theimer, Maria Luisa, Yu, Mei-ying W., Alter, Harvey J., ...
Neutralizing antibodies directed against hepatitis C virus (HCV) are present in Igs made from anti-HCV-positive plasma. However, these HCV-specific Igs are largely ineffective in vivo. The mechanism...